To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig （WZSP） population, we examined the partial exon 1, completed exon 2, and partial exon 3 of SLA-3 loci using the reverse transcription-polym...To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig （WZSP） population, we examined the partial exon 1, completed exon 2, and partial exon 3 of SLA-3 loci using the reverse transcription-polymerase chain reaction （RT- PCR） and the sequencing-based method in 32 WZSPs. According to pedigree and amplification results, PCR products of 8 WZSPs were selected to clone and sequence. Nine different nucleotide sequences were obtained. After comparing the DNA and protein sequences of the WZSPs SLA-3 alleles with the published GenBank SLA sequences, it was found that the SLA-3 alleles in WZSPs were all novel, but there were very few variations among them. Comparision of SLA-3 and HLA-A protein sequences indicated that there was more sequence homology. Meanwhile, the construction of a phylogenetic tree using the nucleotide sequences of 23 SLA-3 alleles and 1 HLA-A allele represented that the WZSP population owns its unique genetics resource. In this study, the alleles of SLA-3 on WZSP group were successfully detected and analyzed, which provided the firm basis on the genotype of SLA-3 for breeding specific haplotypes WZSPs.展开更多
The study deals with the effect of native tongue in SLA(second language acquisition). As is well-known, learning a second language is a complicated process, which is affected by a series of interrelated factors. Among...The study deals with the effect of native tongue in SLA(second language acquisition). As is well-known, learning a second language is a complicated process, which is affected by a series of interrelated factors. Among these factors, the native or first language of a second language learner also plays an important role. Owning to this, the study of the role of L1(the first language) in SLA is inevitable. Many studies have been carried out in this field and the results are inconclusive. The study aims at making an overview of the various conclusions of the preceded studies and each previous assertion or conclusion is supported by its corresponding empirical studies. Equally speaking, the paper is going to solve the problem that what is the exact role of native language in SLA and the personal opinion of the researcher is provided as a conclusion of this study.展开更多
文摘To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig （WZSP） population, we examined the partial exon 1, completed exon 2, and partial exon 3 of SLA-3 loci using the reverse transcription-polymerase chain reaction （RT- PCR） and the sequencing-based method in 32 WZSPs. According to pedigree and amplification results, PCR products of 8 WZSPs were selected to clone and sequence. Nine different nucleotide sequences were obtained. After comparing the DNA and protein sequences of the WZSPs SLA-3 alleles with the published GenBank SLA sequences, it was found that the SLA-3 alleles in WZSPs were all novel, but there were very few variations among them. Comparision of SLA-3 and HLA-A protein sequences indicated that there was more sequence homology. Meanwhile, the construction of a phylogenetic tree using the nucleotide sequences of 23 SLA-3 alleles and 1 HLA-A allele represented that the WZSP population owns its unique genetics resource. In this study, the alleles of SLA-3 on WZSP group were successfully detected and analyzed, which provided the firm basis on the genotype of SLA-3 for breeding specific haplotypes WZSPs.
文摘The study deals with the effect of native tongue in SLA(second language acquisition). As is well-known, learning a second language is a complicated process, which is affected by a series of interrelated factors. Among these factors, the native or first language of a second language learner also plays an important role. Owning to this, the study of the role of L1(the first language) in SLA is inevitable. Many studies have been carried out in this field and the results are inconclusive. The study aims at making an overview of the various conclusions of the preceded studies and each previous assertion or conclusion is supported by its corresponding empirical studies. Equally speaking, the paper is going to solve the problem that what is the exact role of native language in SLA and the personal opinion of the researcher is provided as a conclusion of this study.
