Resistance to salt stresses by aerobic granular sludge:sludge property and microbial community

Saline wastewater is regarded as a challenge for wastewater treatment plants because high-salinity conditions negatively affect on traditional biological technologies.Aerobic granular sludge(AGS)has gained attention as a promising technology for saline wastewater treatment because of its compact structure and the ability to withstand toxic loadings.Therefore,this study investigated the saltresistance performance,sludge properties and microbial community of AGS under low-salinity and high-salinity conditions,with the saline concentrations ranging from 0 to 50 g/L.The results showed that AGS could withstand long-term saline stresses,and the maximum salinity reached 50 g/L within 113 d.Under salinities of 10,30,and 50 g/L,the chemical oxygen demand(COD)removal efficiencies were 90.3%,88.0%and 78.0%,respectively.AGS also its maintained strength and aggregation at salinities of 10 and 30 g/L.Overproduction of extracellular polymeric substances(EPS)by non-halophilic bacteria that enhanced sludge aggregation.The compact structure that ensured the microorganisms bioactivity helped to remove organic matters under salinities of 10 and 30 g/L.At a salinity of 50 g/L,moderately halophilic bacteria,including Salinicola,Thioclava,Idiomarina and Albirhodobacter,prevailed in the reactor.The dominant microbial communities shifted to moderately halophilic bacteria,which could maintain aerobic granular stabilization and remove organic matters under 50 g/L salinity.These results in this study provide a further explanation for the long-term operation of AGS for treating saline wastewater at different salinities.It is hoped that this work could bring some clues for the mystery of salt-resistance mechanisms.

Sludge Bulking Property of Membrane Bio-reactor in Albumen Wqastewater Treatment

Albumen wastewater was treated by Membrane Bio-reactor. Sludge bulking property of Membrane Bio-Reactor was investigated in this study through contrast research. When the sludge bulking appeared, the removal efficiency of COD in Membrane Bio-reactor increased slightly under the function of filamentous bacteria. However, the negative effects of the higher net water-head differential pressures,the higher block rate of membrane pore and the great quantity of filamentous bacteria at the externalsurface pres...

Activated Sludge Ozonation to Reduce Sludge Production in MBR

The total experimental period was divided into two stages. At the first stage, a series of batch studies were carried out to get an understanding of the effect of ozouation on sludge properties. At the following stages, three MBRs with different amounts of activated sludge to be ozonated were run in parallel for a long period to evaluate the influence of sludge ozonation on sludge yield and permeate quality. Through batch study, it was found that ozone could disrupt the cell walls and caused the release of plasm from the cells, then the amounts of soluble organics in the solution increased with ozouation time. With the rise of soluble organics, the amount of soluble organics to be mineralized increased as well, which would reduce the soluble organics content. For the counteraction between these two aspects, a pseudo-balanee could be achieved, and soluble organics would vary in a limited range. Sludge ozonation also increased the contents of nitrogen and phosphorus in the solution. In addition, ozouation was effective in improving sludge settling property. On the basis of batch study, a suitable ozone dosage of 0.16 kgO3/kgMLSS was determined. Three systems were run in parallel for a total period of 39 days, it was demonstrated that a part of activated sludge ozonation could reduce sludge production significantly, and biological perfonnanee of mineralization and nitrification would not be inhibited due to sludge ozouation. Experimental results proved that the combination of ozonation unit with MBR unit could achieve an excellent quality of permeate as well as a small quantity of sludge production, and economic analysis indicated that an additional ozonation operating cost for treatment of both wastewater and sludge was only 0.096Yuan （US$ 0.011,5 ）/m^3 wastewater.

Negative Effects of Sludge Bulking in Membrane Bio-Reactor

Sludge bulking property of membrane bio-reactor was investigated in this study through contrast research. When the sludge bulking appeared, the removal efficiency of COD in membrane bio-reactor increased slightly through the function of filamentous bacteria. However, the negative effects of the higher net water-head differential pressures, the high block rate of membrane pore and the great quantity of filamentous bacteria at the external surface presented at the same time. Thus, plenty of methods should be performed to control sludge bulking once it happened in membrane bio-reactor.

Evaluation of activated sludge properties’changes in industrial-wastewater pre-treatment:role of residual aluminum hydrolyzed species with different polymerization degree

With the widespread introduction of pre-coagulation prior to the biological unit in various industrial wastewater treatments,it is noteworthy that long-term accumulation of residual coagulants has certains effect on both micro and macro characteristics of activated sludge(AS).In this study,the morphology distributions of residual aluminum salts(RAS)and their effects on the removal efficiency of AS were investigated under different PAC concentrations.The results showed that the dominance of medium polymeric RAS,formed under an appropriate PAC dose of 20 mg/L enhanced the hydrophobicity,flocculation,and sedimentation performances of AS,as well as the enzymatic activity in cells in the sludge system,improving the main pollutants removal efficiency of the treatment system.Comparatively the species composition with monomer and dimer/high polymer RAS as the overwhelming parts under an over-dosed PAC concentration of 55 mg/L resulted in excessive secretion of EPS with loose flocs structure and conspicuous inhibition of cellular activity,leading to the deterioration of physico-chemical and biological properties of AS.Based on these findings,this study can shed light on the role of the RAS hydrolyzed species distributions,closely relevant to Al dosage,in affecting the comprehensive properties of AS and provide a theoretical reference for coagulants dosage precise control in the pretreatment of industrial wastewater.

Effects of Al^3+ on the microstructure and bioflocculation of anoxic sludge

The aluminum ions generated from mining aluminum,electrolytic aluminum and the industrial production of aluminum-based coagulants(such as AlCl3 and Al2(SO4)3)enter sewage treatment plants and interact with activated sludges.An anaerobic/anoxic/oxic(A2^O)process was used to reveal the effects of Al^3+on the pollutant removal efficiencies,bioflocculation and the micro structure of sludge.The results showed that a low concentration of Al^3+improved the pollutant removal efficiencies and increased the sludge particle size.However,a high concentration of Al^3+hindered microbial flocculation and reduced the pollutant removal efficiencies.With a 10 mg/L Al^3+addition,the chemical oxygen demand(COD),total nitrogen(TN)and NH4+-N increased by 3%,16%and 27%,and reached as high as 68%,60%and 87%,respectively.At the same time,the dehydrogenase activity,flocculation ability(FA)and contact angle of the sludge reached their maximum levels at 41.3 mg/L/hr,45%and 79.63°,respectively.The specific surface area of the sludge decreased to 7.084 m2/g and the sludge pore size distribution shifted to concentrate in the me soporous range.Most of Al^3+was adsorbed on the surface of sludge,changing the physicochemical properties and physical structure of the sludge.

Influence of aeration intensity on the performance of A/0-type sequencing batch MBR system treating azo dye wastewater

Among the numerous parameters affecting the membrane bioreactor(MBR)performance,the aeration intensity is one of the most important factors.In the present investigation,an anoxic/aerobic-type(A/O-type)sequencing batch MBR system,added anoxic process as a pretreatment to improve the biodegradability of azo dye wastewater,was investigated under different aeration intensities and the impact of the aeration intensity on effluent quantity,sludge properties,extracellular polymeric substances(EPS)amount generated as well as the change of permeation flux were examined.Neither lower nor higher aeration intensities could improve A/O-type sequencing batch MBR performances.The results showed 0.15 m^(3)·h^(-1)aeration intensity was promising for treatment of azo dye wastewater under the conditions examined.Under this aeration intensity,chemical oxygen demand(COD),ammonium nitrogen and color removal as well as membrane flux amounted to 97.8%,96.5%,98.7%and 6.21 L·m^(-2)·h^(-1),respectively.The effluent quality,with 25.0 mg·L^(-1)COD,0.84 mg·L^(-1)ammonium nitrogen and 8 chroma,could directly meet the reuse standard in China.In the meantime,the sludge relative hydrophobicity,the bound EPS,soluble EPS and EPS amounts contained in the membrane fouling layer were 70.3%,52.0 mg·g^(-1)VSS,38.8 mg·g^(-1)VSS and 90.8 mg·g^(-1)VSS,respectively,which showed close relationships to both pollutant removals and membrane flux.