[Objective] The aim was to study the effects of over-expressed sm-Ngt1,a glycosyltransferase gene induced by both methyl jasmonate and salicylic acid from tobacco,on the plant height of rice.[Method] The binary expres...[Objective] The aim was to study the effects of over-expressed sm-Ngt1,a glycosyltransferase gene induced by both methyl jasmonate and salicylic acid from tobacco,on the plant height of rice.[Method] The binary expression vector of the sm-Ngt1 gene was constructed and transferred to matured embryo of indica rice YTB with the method of Agrobacterium infection.The height of the positive transgenic plants was measured.[Result] 117 positive transgenic plants with sm-Ngt1 were obtained.The results showed that the rice plants dwarfed with different degrees after transferring the sm-Ngt1 gene,the height of 37% of transgenic plants is 71.4±9.8 cm,27% is 65.1±4.6 cm,and the average height of YTB（CK） is 130.0±4.3 cm.[Conclusion] These results aid a foundafion for further study on function of sm-Ngt1.展开更多
基金Supported by National Science Foundation of China(30871318)Key Research Project of Ministry of Education,PRC(210265)~~
文摘[Objective] The aim was to study the effects of over-expressed sm-Ngt1,a glycosyltransferase gene induced by both methyl jasmonate and salicylic acid from tobacco,on the plant height of rice.[Method] The binary expression vector of the sm-Ngt1 gene was constructed and transferred to matured embryo of indica rice YTB with the method of Agrobacterium infection.The height of the positive transgenic plants was measured.[Result] 117 positive transgenic plants with sm-Ngt1 were obtained.The results showed that the rice plants dwarfed with different degrees after transferring the sm-Ngt1 gene,the height of 37% of transgenic plants is 71.4±9.8 cm,27% is 65.1±4.6 cm,and the average height of YTB（CK） is 130.0±4.3 cm.[Conclusion] These results aid a foundafion for further study on function of sm-Ngt1.
