Salt stress adversely impacts crop production.Several spliceosome components have been implicated in regulating salt stress responses in plants,however,the underlying molecular basis is still unclear.Here we report th...Salt stress adversely impacts crop production.Several spliceosome components have been implicated in regulating salt stress responses in plants,however,the underlying molecular basis is still unclear.Here we report that the spliceosomal core protein SmEb is essential to salt tolerance in Arabidopsis.Transcriptome analysis showed that SmEb modulates alternative splicing of hundreds of pre-mRNAs in plant response to salt stress.Further study revealed that SmEb is crucial in maintaining proper ratio of two RCD1 splicing variants(RCD1.1/RCD1.2)important for salt stress response.In addition,RCD1.1 but not RCD1.2 is able to interact with the stress regulators and attenuates saltsensitivity by decreasing salt-induced cell death in smeb-1 mutant.Together,our findings uncovered the essential role of SmEb in the regulation of alternative pre-mRNA splicing in salt stress response.展开更多
Abscisic acid(ABA)signaling is critical for seed germination and abiotic stress responses in terrestrial plants.PremRNA splicing is known to regulate ABA signaling.However,the involvement of canonical spliceosomal com...Abscisic acid(ABA)signaling is critical for seed germination and abiotic stress responses in terrestrial plants.PremRNA splicing is known to regulate ABA signaling.However,the involvement of canonical spliceosomal components in regulating ABA signaling is poorly understood.Here,we show that the spliceosome component Sm core protein SmEb plays an important role in ABA signaling.SmEb expression is up-regulated by ABA treatment,and analysis of Arabidopsis smeb mutant plants suggest that SmEb modulates the alternative splicing of the ABA signaling component HAB1 by enhancing the HAB1.1 splicing variant while repressing HAB1.2.Overexpression of HAB1.1 but not HAB1.2 rescues the ABA-hypersensitive phenotype of smeb mutants.Mutations in the transcription factor ABI3,4,or 5 also reduce the ABA hypersensitivity of smeb mutants during seed germination.Our results show that the spliceosomal component SmEb plays an important role in ABA regulation of seed germination and early seedling development.展开更多
基金supported by the National Natural Science Foundation of China(32000206 to Z.W.and 32200234 to Y.H.)Anhui Provincial Natural Science Foundation(2208085Y08 to Z.W.)Shenzhen Science and Technology Program(RCBS20210706092213009 to Y.H.)。
文摘Salt stress adversely impacts crop production.Several spliceosome components have been implicated in regulating salt stress responses in plants,however,the underlying molecular basis is still unclear.Here we report that the spliceosomal core protein SmEb is essential to salt tolerance in Arabidopsis.Transcriptome analysis showed that SmEb modulates alternative splicing of hundreds of pre-mRNAs in plant response to salt stress.Further study revealed that SmEb is crucial in maintaining proper ratio of two RCD1 splicing variants(RCD1.1/RCD1.2)important for salt stress response.In addition,RCD1.1 but not RCD1.2 is able to interact with the stress regulators and attenuates saltsensitivity by decreasing salt-induced cell death in smeb-1 mutant.Together,our findings uncovered the essential role of SmEb in the regulation of alternative pre-mRNA splicing in salt stress response.
基金supported by the National Natural Science Foundation of China(grant 32000206 to Z.W.)the Youth Innovation Promotion Association(2020273 to Z.W.)of the Chinese Academy of Sciences.
文摘Abscisic acid(ABA)signaling is critical for seed germination and abiotic stress responses in terrestrial plants.PremRNA splicing is known to regulate ABA signaling.However,the involvement of canonical spliceosomal components in regulating ABA signaling is poorly understood.Here,we show that the spliceosome component Sm core protein SmEb plays an important role in ABA signaling.SmEb expression is up-regulated by ABA treatment,and analysis of Arabidopsis smeb mutant plants suggest that SmEb modulates the alternative splicing of the ABA signaling component HAB1 by enhancing the HAB1.1 splicing variant while repressing HAB1.2.Overexpression of HAB1.1 but not HAB1.2 rescues the ABA-hypersensitive phenotype of smeb mutants.Mutations in the transcription factor ABI3,4,or 5 also reduce the ABA hypersensitivity of smeb mutants during seed germination.Our results show that the spliceosomal component SmEb plays an important role in ABA regulation of seed germination and early seedling development.
