The purposes of this research is to investigate Smad2, phosphorylated Smad2 (p-Smad2) and type Ⅰ collagen expressions in the cultured human Tenon′s capsular fibroblasts (HTFs) transfected with Smad7 vector and to el...The purposes of this research is to investigate Smad2, phosphorylated Smad2 (p-Smad2) and type Ⅰ collagen expressions in the cultured human Tenon′s capsular fibroblasts (HTFs) transfected with Smad7 vector and to elucidate the mechanism of Smad7 in blocking tissue fibrosis after filtration surgery. NucleofectorTM was used to transfect Smad7 vector into HTFs. Reverse transcription real-time quantitive polymerase chain reaction (real-time RT-PCR) was then used to detect Smad7 mRNA expression levels. The expressions of HA-probe fusion protein, Smad2, p-Smad2, α2-type Ⅰ procollagen (COL1A2) mRNA and carboxyterminal propeptide of type Ⅰ procollagen (PICP) were determined by real-time RT-PCR, Western blot, cellular immunofluorescence assay and radioimmunoassay. The items mentioned above were also detected after HTFs being stimulated by TGF-β2 (10ng/ml). HTFs and vector-HTFs were used as control groups. Clones with Smad7 overexpression were successfully established. Results of real-time RT-PCR proved that there was no difference of Smad2 mRNA between Smad7 overexpression clone and the control groups whether stimulated by TGF-β2 or not. The increase of p-Smad2 expression stimulated by TGF-β2 was inhibited in Smad7 overexpression clone by Western blot assay. It only took 56.01% and 53.48% of control groups. In cellular immunofluorescence assay, the p-Smad2 positive cells in Smad7 overexpression clone only took 31.02% and 29.41% of control groups. Smad7-HTFs could also abolish the increase of COL1A2 mRNA expression and PICP concentration stimulated by TGF-β2. It is possible that Smad7 can block the signal transduction of TGF-β by downregulating the expression of p-Smad2 in HTFs. However, it doesn’t affect the expression of Smad2.展开更多
文摘The purposes of this research is to investigate Smad2, phosphorylated Smad2 (p-Smad2) and type Ⅰ collagen expressions in the cultured human Tenon′s capsular fibroblasts (HTFs) transfected with Smad7 vector and to elucidate the mechanism of Smad7 in blocking tissue fibrosis after filtration surgery. NucleofectorTM was used to transfect Smad7 vector into HTFs. Reverse transcription real-time quantitive polymerase chain reaction (real-time RT-PCR) was then used to detect Smad7 mRNA expression levels. The expressions of HA-probe fusion protein, Smad2, p-Smad2, α2-type Ⅰ procollagen (COL1A2) mRNA and carboxyterminal propeptide of type Ⅰ procollagen (PICP) were determined by real-time RT-PCR, Western blot, cellular immunofluorescence assay and radioimmunoassay. The items mentioned above were also detected after HTFs being stimulated by TGF-β2 (10ng/ml). HTFs and vector-HTFs were used as control groups. Clones with Smad7 overexpression were successfully established. Results of real-time RT-PCR proved that there was no difference of Smad2 mRNA between Smad7 overexpression clone and the control groups whether stimulated by TGF-β2 or not. The increase of p-Smad2 expression stimulated by TGF-β2 was inhibited in Smad7 overexpression clone by Western blot assay. It only took 56.01% and 53.48% of control groups. In cellular immunofluorescence assay, the p-Smad2 positive cells in Smad7 overexpression clone only took 31.02% and 29.41% of control groups. Smad7-HTFs could also abolish the increase of COL1A2 mRNA expression and PICP concentration stimulated by TGF-β2. It is possible that Smad7 can block the signal transduction of TGF-β by downregulating the expression of p-Smad2 in HTFs. However, it doesn’t affect the expression of Smad2.
