The research analyzed the expression and difference of MSTN/Smad signal path genes(MSTN, Smad2, Smad3,Smad4, TGFBR1, TGFBR2) in Turpan black sheep muscle tissue, as well as the correlation with some growth indices. Be...The research analyzed the expression and difference of MSTN/Smad signal path genes(MSTN, Smad2, Smad3,Smad4, TGFBR1, TGFBR2) in Turpan black sheep muscle tissue, as well as the correlation with some growth indices. Besides,the research used the fluorescence quantitative PCR technique to detect the expression level of MSTN, Smad2, Smad3, Smad4,TGFBR1 and TGFBR2 genes in m RNA of Turpan black sheep muscle. The research also used conventional and histological methods to determine the muscle growth and meat producibility indices, discussed the relationship between MSTN/Smad signal path and muscle growth and meat production. The result shows that these genes are expressed in Turpan black sheep muscle tissues, and have some relationship with body weight, height, length, chest circumference, hoof circumference, carcass and dressing percentage. The MSTN gene is negatively correlated with body weight(r = 0.993, P < 0.05), and hoof circumference(r= 0.957, p < 0.01), but has no significant correlation with body height, length, carcass weight, dressing percentages(P > 0.05);Smad2 gene shows significantly negative correlation the with dressing percentage(r = 0.918, p < 0.05), but no significant correlation with body weight, height, length, chest circumference, hoof circumference and carcass(P > 0.05); Smad3 gene shows no significant correlation with any of them(P > 0.05); Smad4, TGFBR1, TGFBR2 did not show any significant relationship with body height, length, chest circumference, weight and dressing percentage(P > 0.05).展开更多
目的：细胞移植技术的探索，为急性心肌梗死患者坏死区的心肌细胞重建及衰竭心脏的功能恢复，可能是-种极具前途的临床治疗手段。我们前期的研究初步证实巴戟天寡糖（Morinda officinalis How oligosaccha rides，MOO）具有明显促进成...目的：细胞移植技术的探索，为急性心肌梗死患者坏死区的心肌细胞重建及衰竭心脏的功能恢复，可能是-种极具前途的临床治疗手段。我们前期的研究初步证实巴戟天寡糖（Morinda officinalis How oligosaccha rides，MOO）具有明显促进成肌细胞的增殖和分化的作用保护心脏作用的研究。方法：采用离体纯化培养乳鼠双侧后肢骨骼肌成肌细胞的方法，以5-氮杂2’-脱氧胞苷（5-Aza—dc）为阳性药对照组、并设立MOO中剂量＋5-Aza—dc联合用药组，应用免疫组化、蛋白免疫痕迹技术进-步观察了MOO促成肌细胞向心肌样细胞分化时对TGF-β2受体及信号传导的影响。结果：（1）与空白对照组相比，各组TGF-β2表达水平均上调，差异显著（P〈0．05）；与阳性对照组相比，MOO各剂量组TGF—β2表达水平随剂量增加而增强，联合用药组TGF-β2表达水平显著高于阳性对照组（P〈0．05）。（2）与空白对照组相比，各用药组Smad4表达水平均显著上调（P〈0．05），各用药组间Smad4表达水平无明显差异。（3）与空白对照组相比，MOO小剂量组及联合用药组Smad2／3表达轻微上调、MOO中、大剂量组及阳性组均下调Smad2／3的表达。（4）与空白对照组相比，各组Smad7的表达差异显著（P〈0．05），其中MOO小剂量组、阳性组和联合组均抑制Smad7表达，MOO中、大剂量组逐渐增强Smad7的表达。结论：（1）MOO可促进TGF-β2的表达，并随着剂量的增加表达增强。展开更多
文摘The research analyzed the expression and difference of MSTN/Smad signal path genes(MSTN, Smad2, Smad3,Smad4, TGFBR1, TGFBR2) in Turpan black sheep muscle tissue, as well as the correlation with some growth indices. Besides,the research used the fluorescence quantitative PCR technique to detect the expression level of MSTN, Smad2, Smad3, Smad4,TGFBR1 and TGFBR2 genes in m RNA of Turpan black sheep muscle. The research also used conventional and histological methods to determine the muscle growth and meat producibility indices, discussed the relationship between MSTN/Smad signal path and muscle growth and meat production. The result shows that these genes are expressed in Turpan black sheep muscle tissues, and have some relationship with body weight, height, length, chest circumference, hoof circumference, carcass and dressing percentage. The MSTN gene is negatively correlated with body weight(r = 0.993, P < 0.05), and hoof circumference(r= 0.957, p < 0.01), but has no significant correlation with body height, length, carcass weight, dressing percentages(P > 0.05);Smad2 gene shows significantly negative correlation the with dressing percentage(r = 0.918, p < 0.05), but no significant correlation with body weight, height, length, chest circumference, hoof circumference and carcass(P > 0.05); Smad3 gene shows no significant correlation with any of them(P > 0.05); Smad4, TGFBR1, TGFBR2 did not show any significant relationship with body height, length, chest circumference, weight and dressing percentage(P > 0.05).
文摘目的：细胞移植技术的探索，为急性心肌梗死患者坏死区的心肌细胞重建及衰竭心脏的功能恢复，可能是-种极具前途的临床治疗手段。我们前期的研究初步证实巴戟天寡糖（Morinda officinalis How oligosaccha rides，MOO）具有明显促进成肌细胞的增殖和分化的作用保护心脏作用的研究。方法：采用离体纯化培养乳鼠双侧后肢骨骼肌成肌细胞的方法，以5-氮杂2’-脱氧胞苷（5-Aza—dc）为阳性药对照组、并设立MOO中剂量＋5-Aza—dc联合用药组，应用免疫组化、蛋白免疫痕迹技术进-步观察了MOO促成肌细胞向心肌样细胞分化时对TGF-β2受体及信号传导的影响。结果：（1）与空白对照组相比，各组TGF-β2表达水平均上调，差异显著（P〈0．05）；与阳性对照组相比，MOO各剂量组TGF—β2表达水平随剂量增加而增强，联合用药组TGF-β2表达水平显著高于阳性对照组（P〈0．05）。（2）与空白对照组相比，各用药组Smad4表达水平均显著上调（P〈0．05），各用药组间Smad4表达水平无明显差异。（3）与空白对照组相比，MOO小剂量组及联合用药组Smad2／3表达轻微上调、MOO中、大剂量组及阳性组均下调Smad2／3的表达。（4）与空白对照组相比，各组Smad7的表达差异显著（P〈0．05），其中MOO小剂量组、阳性组和联合组均抑制Smad7表达，MOO中、大剂量组逐渐增强Smad7的表达。结论：（1）MOO可促进TGF-β2的表达，并随着剂量的增加表达增强。
