AIM： TO investigate the effects of the somatostatin analogue, octreotide, on maltose and sucrase activities and expression of glucose transporter type 2 （GLUT2） in obese rat intestinal mucosa. METHODS： We divided ...AIM： TO investigate the effects of the somatostatin analogue, octreotide, on maltose and sucrase activities and expression of glucose transporter type 2 （GLUT2） in obese rat intestinal mucosa. METHODS： We divided 49 Sprague-Dawley rats into a group of 31 high fat diet-induced obese rats and a group of 18 normal controls. The obese rats were separated into an octreotide treated group 9f 16 rats and an obese group of 15. The intervention （：jroup was injected with octreotide at 40 ±g/kg body weight every 12 h for 8 d. Rat body weight was measured weekly to calculate Lee＇s index. After euthanization, maltase and sucrase activities in the small intestine were measured by activity assays, and the fasting plasma glucose level was measured. The expression of GLUT2 in small intestinal mucosa was analyzed by immunohistochemistry, reverse transcriptase polymerase chain reaction and Western blotting assays. RESULTS： Body weight, Lee＇s index, fasting plasma glucose level, maltase activity in small intestinal mucosa, mucosa and apical GLUT2, GLUT2 mRNA and protein expression levels were all significantly higher in the obese group than in the normal control group （605.61 ± 141.00 vs 378.54 ±111.75, 337.61 ± 10.82 vs 318.73 ± 20.10, 8.60± 1.38 vs 7.33 ± 0.70, 156.01 ± 58.81 vs 50.43 ± 30.49, 390 744.2± 62 469.21 vs 170 546.50 ± 50 646.14, 26 740.18 ±3809.60 vs 354.98± 57.19, 0.26± 0.11 vs 0.07± 0.02, and 2.08 ± 0.59 vs 1.27 ± 0.38, respectively, all P 〈 0.01）. Sucrase activity did not differ between the two groups. Octreotide intervention significantly decreased the body weight and fasting plasma glucose level of obese rats （508.27 ± 94.39 vs 605.61 ± 141.00, 7.58 ± 1.51 vs 8.60±1.38, respectively, all P 〈 0.05）. The intestinal mucosa and apical GLUT2, expression of GLUT2 mRNA and protein were also significantly lower in the octreotide intervention group than in the obese group （269 975.2 ± 53 730.94 vs 390 744.2 ± 62 469.21, 3758.06 ± 364.51 vs 26 740.18 ± 3809.60, 0.08 ± 0.02 vs 0.26 ±0.11, and 1.31 ± 0.27 vs 2.08 ±0.59, respectively, all P 〈 0.01）. CONCLUSION： High fat dietinduced obesity is associated with elevated intestinal maltase activity, GLUT2 expression, and permanent apical GLUT2 in the small intestinal mucosa of rats. Octreotide can inhibit these effects.展开更多
目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除...目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除空白对照组外,其他组大鼠均给予高脂饲料建立肥胖模型。苦瓜桑叶复合颗粒低、高剂量组每天分别灌胃0.5和1 g/kg的苦瓜桑叶复合颗粒,其他组以相同方法灌胃等体积生理盐水,连续给药8周。测定大鼠体重及血糖血脂水平,包括葡萄糖(glucose,GLU)、糖化血清蛋白(glycated serum proteins,GSP)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid-cholesterol,LDL-C)等。通过苏木精-伊红染色观察大鼠肝脏和结肠组织形态变化,并检测结肠紧密连接蛋白Occludin的表达。结果:苦瓜桑叶复合颗粒可有效调控高脂饮食诱导肥胖大鼠的体重和血糖血脂水平,使其恢复正常。病理切片显示苦瓜桑叶复合颗粒可降低肥胖大鼠肝脏脂滴的形成并改善结肠粘膜腺体异常形态。同时,苦瓜桑叶复合颗粒可有效提升肥胖大鼠结肠Occludin蛋白的表达(P<0.05),改善肠粘膜紧密连接状态。结论:苦瓜桑叶复合颗粒对高脂饮食诱导的肥胖大鼠脂糖代谢异常具有明显改善作用,并可一定程度修复肥胖引起的肠道粘膜损伤,此研究对苦瓜桑叶复合颗粒的开发及相关机制的阐明具有重要意义。展开更多
基金Supported by Grants from the National Natural Sciences Foundation of China,No.30870919Sichuan Provincial Department of Science and Technology,No.2010SZ0176
文摘AIM： TO investigate the effects of the somatostatin analogue, octreotide, on maltose and sucrase activities and expression of glucose transporter type 2 （GLUT2） in obese rat intestinal mucosa. METHODS： We divided 49 Sprague-Dawley rats into a group of 31 high fat diet-induced obese rats and a group of 18 normal controls. The obese rats were separated into an octreotide treated group 9f 16 rats and an obese group of 15. The intervention （：jroup was injected with octreotide at 40 ±g/kg body weight every 12 h for 8 d. Rat body weight was measured weekly to calculate Lee＇s index. After euthanization, maltase and sucrase activities in the small intestine were measured by activity assays, and the fasting plasma glucose level was measured. The expression of GLUT2 in small intestinal mucosa was analyzed by immunohistochemistry, reverse transcriptase polymerase chain reaction and Western blotting assays. RESULTS： Body weight, Lee＇s index, fasting plasma glucose level, maltase activity in small intestinal mucosa, mucosa and apical GLUT2, GLUT2 mRNA and protein expression levels were all significantly higher in the obese group than in the normal control group （605.61 ± 141.00 vs 378.54 ±111.75, 337.61 ± 10.82 vs 318.73 ± 20.10, 8.60± 1.38 vs 7.33 ± 0.70, 156.01 ± 58.81 vs 50.43 ± 30.49, 390 744.2± 62 469.21 vs 170 546.50 ± 50 646.14, 26 740.18 ±3809.60 vs 354.98± 57.19, 0.26± 0.11 vs 0.07± 0.02, and 2.08 ± 0.59 vs 1.27 ± 0.38, respectively, all P 〈 0.01）. Sucrase activity did not differ between the two groups. Octreotide intervention significantly decreased the body weight and fasting plasma glucose level of obese rats （508.27 ± 94.39 vs 605.61 ± 141.00, 7.58 ± 1.51 vs 8.60±1.38, respectively, all P 〈 0.05）. The intestinal mucosa and apical GLUT2, expression of GLUT2 mRNA and protein were also significantly lower in the octreotide intervention group than in the obese group （269 975.2 ± 53 730.94 vs 390 744.2 ± 62 469.21, 3758.06 ± 364.51 vs 26 740.18 ± 3809.60, 0.08 ± 0.02 vs 0.26 ±0.11, and 1.31 ± 0.27 vs 2.08 ±0.59, respectively, all P 〈 0.01）. CONCLUSION： High fat dietinduced obesity is associated with elevated intestinal maltase activity, GLUT2 expression, and permanent apical GLUT2 in the small intestinal mucosa of rats. Octreotide can inhibit these effects.
文摘目的:研究苦瓜桑叶复合颗粒对高脂饮食诱导肥胖大鼠脂糖代谢及肠粘膜屏障异常的改善作用,为苦瓜桑叶复合颗粒的进一步开发应用提供数据支持。方法:SD大鼠随机分为空白对照组、高脂肥胖模型组、苦瓜桑叶复合颗粒低剂量组和高剂量组。除空白对照组外,其他组大鼠均给予高脂饲料建立肥胖模型。苦瓜桑叶复合颗粒低、高剂量组每天分别灌胃0.5和1 g/kg的苦瓜桑叶复合颗粒,其他组以相同方法灌胃等体积生理盐水,连续给药8周。测定大鼠体重及血糖血脂水平,包括葡萄糖(glucose,GLU)、糖化血清蛋白(glycated serum proteins,GSP)、总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipid-cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipid-cholesterol,LDL-C)等。通过苏木精-伊红染色观察大鼠肝脏和结肠组织形态变化,并检测结肠紧密连接蛋白Occludin的表达。结果:苦瓜桑叶复合颗粒可有效调控高脂饮食诱导肥胖大鼠的体重和血糖血脂水平,使其恢复正常。病理切片显示苦瓜桑叶复合颗粒可降低肥胖大鼠肝脏脂滴的形成并改善结肠粘膜腺体异常形态。同时,苦瓜桑叶复合颗粒可有效提升肥胖大鼠结肠Occludin蛋白的表达(P<0.05),改善肠粘膜紧密连接状态。结论:苦瓜桑叶复合颗粒对高脂饮食诱导的肥胖大鼠脂糖代谢异常具有明显改善作用,并可一定程度修复肥胖引起的肠道粘膜损伤,此研究对苦瓜桑叶复合颗粒的开发及相关机制的阐明具有重要意义。
