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Network pharmacology and molecular docking techniques to explore the mechanism of Smilax china L.in the treatment of myocardial infarction
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作者 Qing Lan Peng-Yu Wang +4 位作者 Shuo Zhang Qiao-Yun Zhang Tong Wang Guan-Di Ma You-Zhi Zhang 《Precision Medicine Research》 2023年第1期15-24,共10页
Objective:To predict the relevant targets and signaling pathways of Smilax china L.(SC)for treating myocardial infarction on the basis of network pharmacology and molecular docking.Consequently,the basis for additiona... Objective:To predict the relevant targets and signaling pathways of Smilax china L.(SC)for treating myocardial infarction on the basis of network pharmacology and molecular docking.Consequently,the basis for additional in-depth investigation is obtained.Methods:First,the targets of SC and the targets for treating myocardial infarction were screened from different databases,Then the intersection genes of SC for treating myocardial infarction were performed in Venny 2.1.0.Second,to obtain the protein interaction network,the Metascape database,String database,were used to analyze the important modules related to the signaling pathway using MCODE algorithm.Furthermore,the DAVID database was used for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.And we constructed the“interaction targets-pathways”network by Cytoscape software,and using Network Analyzer was used to screen the core compound,core targets and core pathways.Finally,molecular docking was used to verify whether the core compounds and core targets had better docking binding.Results:11 active ingredients and 98 targets of SC,1846 targets to treat myocardial infarction and 58 targets related to treat myocardial infarction in SC were obtained;MCODE analysis of the protein-protein interaction network yielded 4 important modules related to signaling pathways;Gene Ontology enrichment analysis yielded 848 entries and Kyoto Encyclopedia of Genes and Genomes enrichment analysis yielded 144 signaling pathways;the core compounds were beta-sitosterol,diosgenin,kaempferol,core targets were AKT1,CASP9,BCL2,core pathways were pathways in cancer,pathways of neurodegeneration-multiple diseases,Kaposi’s sarcoma-associated herpesvirus infection,lipid and atherosclerosis and human cytomegalovirus infection.Finally,molecular docking between core components and core targets was verified.Conclusion:The preliminary prediction of the mechanism of the SC in the treatment of myocardial infarction is that it acts through a multi-compounds,multi-targets and multi-pathways.This study provided a theoretical basis and research direction for the mechanism of action of SC in the treatment of myocardial infarction,and lays the foundation for further research on SC in the treatment of myocardial infarction. 展开更多
关键词 smilax china l. myocardial infarction network pharmacology molecular docking
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Screening of flavonoid "quercetin" from the rhizome of Smilax china Linn.for anti-psoriatic activity 被引量:7
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作者 Vijayalakshmi A Ravichandiran V +2 位作者 Malarkodi Velraj Nirmala S Jayakumari S 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2012年第4期269-275,共7页
Objective:To assess anti-psoriatic activity of the methanol extract and the isolated flavonoid quercetin from the rhizome of Smilax china(S.china) Linn.Methods:Mouse tail test was used for the evaluation of anti-psori... Objective:To assess anti-psoriatic activity of the methanol extract and the isolated flavonoid quercetin from the rhizome of Smilax china(S.china) Linn.Methods:Mouse tail test was used for the evaluation of anti-psoriatic activity.Methanol extract(100 and 200 mg/kg b.w.) and isolated flavonoid quercetin(25 and 50 mg/kg b.w.) were tested in Swiss albino mice.Parameters studied in the mouse tail test were changes in epidermal thickness and percentage orthokeratotic values.The anti-inflammatory role of the methanol extract and isolated flavonoid quercetin was evaluated using carrageenan-induced pleurisy in rats.In vitro antiproliferant assay on HaCaT cell lines was also carried out.Results:The isolated flavonoid quercetin from the rhizome of S.china produced significant orthokeratosis(P<0.01) in the mouse tail test.In epidermal thickness,a significant reduction with respect to control was observed in groups treated with retinoic acid and isolated flavonoid quercetin.The methanol extract(200 mg/kg) and isolated flavonoid quercetin(50 mg/kg) showed anti-inflammatory effect in terms of significant inhibition(P<0.001) in leukocyte migration.Maximum antiproliferant activity was shown by isolated flavonoid quercetin(IC_(50),62.42± 10.20 μg/mL).Conclusions:From the above data,the flavonoid quercetin shows significant orthokeratosis,anti-inflammatory and maximum antiproliferant activities.To our knowledge,this is the first report on the anti-psoriatic effect of the flavonoid quercetin which is promising for further investigations to prove its anti-psoriatic activity. 展开更多
关键词 smilax china Anti-psoriatic QUERCETIN HACAT cells ANTI-INFlAMMATION Flavonoid Orthokeratosis Antiproliferant ACTIVITY RHIZOME
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口蹄疫病毒株China/99L区段核苷酸序列测定及比较分析
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作者 张显升 赵启祖 +7 位作者 刘在新 刘相涛 常惠芸 江鹏斐 陈应理 李冬 魏怀录 谢庆阁 《中国病毒学》 CSCD 2002年第2期158-162,共5页
以口蹄疫病毒株China/ 99RNA为模板 ,反转录并扩增目的cDNA ,然后与 pGEM TEasy载体连接并转化JM10 9菌株 ,提取的重组质粒用电泳、PCRampos和EcoR1酶切法鉴定。该毒株与A10、O1K、O1Campos和TW 45毒株的核苷酸序列差异率分别为 15 .2 7... 以口蹄疫病毒株China/ 99RNA为模板 ,反转录并扩增目的cDNA ,然后与 pGEM TEasy载体连接并转化JM10 9菌株 ,提取的重组质粒用电泳、PCRampos和EcoR1酶切法鉴定。该毒株与A10、O1K、O1Campos和TW 45毒株的核苷酸序列差异率分别为 15 .2 7%、15 .5 6 %、15 .5 6 %和 15 .49% ;氨基酸序列差异率分别为 8.2 9%、8.76 %、9.2 2 %和 10 .14%。五个毒株的L/P1连接处均为苷氨酸 (Gly) /异亮氨酸 (Ile)。序列比较表明 ,T C、A G和A C转换率较高 ,是点突变的热点核苷酸 ,是影响氨基酸稳定的因素之一。第 43 5 3、95 10 5、10 8 111、146 15 3、16 1 173、183 188和 182 187区域极有可能是L蛋白酶的活性中心 ,第 48位的H、5 1的C、6 5位的E、95位的H、10 9位的H、138位的H、148位的H和 16 5位的E可能是L蛋白酶的活性位点 。 展开更多
关键词 口蹄疫病毒株 china/99l区段 核苷酸序列测定 比较分析
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Response of grain yield to plant density and nitrogen rate in spring maize hybrids released from 1970 to 2010 in Northeast China 被引量:19
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作者 Chunrong Qian Yang Yu +7 位作者 Xiujie Gong Yubo Jiang Yang Zhao Zhongliang Yang Yubo Hao Liang Li Zhenwei Song Weijian Zhang 《The Crop Journal》 SCIE CAS CSCD 2016年第6期459-467,共9页
The objective of this study was to identify the response of grain yield to plant density and nitrogen rate in spring maize hybrids released from 1970 to 2010 and grown extensively in Northeast China.Twenty-one hybrids... The objective of this study was to identify the response of grain yield to plant density and nitrogen rate in spring maize hybrids released from 1970 to 2010 and grown extensively in Northeast China.Twenty-one hybrids were grown for 2 years in Northeast China at densities of 30,000,52,500,75,000,and 97,500 plants ha^(-1)and N application levels of 0,150,300,and 450 kg N ha^(-1).Irrespective of density or nitrogen application rate,grain yields both per plant and per unit area were significantly higher for newer than older hybrids.As plant density increased from 30,000 to 97,500 plant ha^(-1),yield per plant of 1970 s,1980 s,1990 s,and 2000 s hybrids decreased by 50%,45%,46%,and 52%,respectively.The response of grain yield per unit area to plant density was curvilinear.The estimated optimum plant densities were about 58,000,49,000,65,000,and 65,000 plants ha^(-1)for hybrids released in the 1970 s,1980s,1990 s,and 2000 s,respectively.The theoretical optimum densities for the hybrids released from the 1970 s to the 2000 s increased by 1750 plants ha^(-1)decade^(-1).Nitrogen fertilization significantly increased grain yields per plant and per unit area for all hybrids.The theoretical optimum N application rates for high yield for hybrids released in the 1970 s and 1980 s were about 280 and 360 kg ha^(-1),and the hybrids from the 1990 s and 2000 s showed highest yield at 330 kg ha^(-1)N.No significant difference in the grain yields of 2000 s hybrids between the N levels of 150 to 450 kg ha^(-1)was found.Significant yield gains per plant and per unit area were found,with average increases of 17.9 g plant^(-1)decade^(-1)and936 kg ha^(-1)decade^(-1)over the period 1970–2010,respectively.Yield gains were attributed mainly to increased yield per plant,contributed by increases in kernel number per ear and1000-kernel weight.The rates of lodging and barren plants of newer hybrids were significantly lower than those of older ones,especially at high plant density. 展开更多
关键词 Zea mays l. Grain yield Plant density Nitrogen application rate Northeast china
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SPEI_(PM)-based research on drought impact on maize yield in North China Plain 被引量:16
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作者 MING Bo GUO Yin-qiao +3 位作者 TAO Hong-bin LIU Guang-zhou LI Shao-kun WANG Pu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第4期660-669,共10页
The calculation method of potential evapotranspiration(PET) was improved by adopting a more reliable PET estimate based on the Penman-Monteith equation into the standardized precipitation evapotranspiration index(S... The calculation method of potential evapotranspiration(PET) was improved by adopting a more reliable PET estimate based on the Penman-Monteith equation into the standardized precipitation evapotranspiration index(SPEI) in this study(SPEI PM). This improvement increased the applicability of SPEI in North China Plain(NCP). The historic meteorological data during 1962–2011 were used to calculate SPEI PM. The detrended yields of maize from Hebei, Henan, Shandong, Beijing, and Tianjin provinces/cities of NCP were obtained by linear sliding average method. Then regression analysis was made to study the relationships between detrended yields and SPEI values. Different time scales were applied, and thus SPEI PM was mentioned as SPEI PMk-j(k=time scale, 1, 2, 3, 4,…, 24 mon; j=month, 1, 2, 3,..., 12), among which SPEI PM3-8 reflected the water condition from June to August, a period of heavy precipitation and vigorous growth of maize in NCP. SPEI PM3-8 was highly correlated with detrended yield in this region, which can effectively evaluate the effect of drought on maize yield. Additionally, this relationship becomes more significant in recent 20 yr. The regression model based on the SPEI series explained 64.8% of the variability of the annual detrended yield in Beijing, 45.2% in Henan, 58.6% in Shandong, and 54.6% in Hebei. Moreover, when SPEI PM3-8 is in the range of –0.6 to 1.1, –0.9 to 0.8 and –0.8 to 2.3, the detrended yield increases in Shandong, Henan and Beijing. The yield increasing range was during normal water condition in Shandong and Henan, where precipitation was abundant. It indicated that the field management matched well with local water condition and thus allowed stable and high yield. Maize yield increase in these two provinces in the future can be realized by further improving water use efficiency and enhancing the stress resistance as well as yield stability. In Hebei and Beijing, the precipitation is less and thus the normal water condition cannot meet the high yield target. Increasing of water input and improving water use efficiency are both strategies for future yield increase. As global climate change became stronger and yield demands increased, the relationship between drought and maize yield became much closer in NCP too. The research of drought monitoring method and strategies for yield increase should be enhanced in the future, so as to provide strong supports for food security and agricultural sustainable development in China. 展开更多
关键词 North china Plain maize(Zea mays l.) DROUGHT climate-determined yield standardized precipitation evapotranspiration index
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2022年10月30日渤海M_(L)4.1地震预测与思考
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作者 冀东普 陈时军 李树鹏 《地震地磁观测与研究》 2023年第2期11-17,共7页
依据近年来我国南北地震带部分地区强震、大震与华北地区中等地震活动的某些相关性特征,结合华北地区地震活动演化特点,以及对异常信度较高的区域异常项目对比与分析,山东地震台测震学与综合预报学科对研究区近期发生的一次中等地震,即2... 依据近年来我国南北地震带部分地区强震、大震与华北地区中等地震活动的某些相关性特征,结合华北地区地震活动演化特点,以及对异常信度较高的区域异常项目对比与分析,山东地震台测震学与综合预报学科对研究区近期发生的一次中等地震,即2022年10月30日渤海海域M_(L)4.1地震作出预测,并指出:近年来南北地震带中南段中部大震发生前后,华北地区中等以上地震多出现长时间平静现象,且平静打破后,其后续ML 4地震分布也有一定规律可循;莱州湾及附近海域存在小震调制比高值异常;渤海海域及附近部分地区M_(L)3地震较活跃,同时,M_(L)3地震能量释放呈逐渐增强态势。希望通过对2022年渤海海域M_(L)4.1地震预测过程的分析与总结,为今后地震研究相关工作提供借鉴。 展开更多
关键词 华北地区 中等地震 地震活动演化 渤海海域M_(l)4.1地震 地震预测
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Effects of Different Simple Cultivation Facilities on Yield and Quality of Over-summer Brassica campestris L. in South China 被引量:2
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作者 梁普兴 杨瑞怡 +3 位作者 李强 高祖江 张晶 梁彪 《Agricultural Science & Technology》 CAS 2017年第12期2354-2356,2377,共4页
The effects of different simple cultivation facilities on yield and quality of Brassica campestris L. were compared to explore the optimal facility type for pro- duction of B. campestris, thereby providing a technical... The effects of different simple cultivation facilities on yield and quality of Brassica campestris L. were compared to explore the optimal facility type for pro- duction of B. campestris, thereby providing a technical reference for efficient cultiva- tion of B. campestris during hot season. A total of four treatments were designed, open field cultivation (CK), ordinary anti-insect net shed cultivation, ordinary plastic film greenhouse cultivation and Japanese translucent anti-insect net shed cultivation. The results showed that compared with those in open field cultivation, the plant height, leaf area, yield, Vc content, soluble sugar content and soluble protein con- tent of B. campestris in Japanese translucent anti-insect net shed cultivation were increased by 22%, 63%, 49%, 14%, 13% and 10%, respectively, and Japanese translucent anti-insect net shed was the optimal facility type for cultivation of B. campestris in hot season. 展开更多
关键词 South china Brassica campestris l. Over-summer cultivation Simplefacility
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Molecular Epidemiology and Sequencing of the G-L Intergenic Region of Rabies Viruses Isolated in China 被引量:8
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作者 Sheng-Li MENG Ge-Lin XU +8 位作者 Jia-Xin YAN Ping-Gang MING Jie WU Xiao-MingYANG He-Tian MING Feng-Cai ZHU Dun-Jin ZHOU QI-You XIAO Guan-Mu DONG 《中国病毒学》 CSCD 2007年第1期26-33,共8页
一组 25 个狂犬病病毒(RABV ) ,从 24 条狗和一个人的盒子恢复了,在在 2004 和 2006 之间的中国从各种各样的区域被收集。G-L intergenic 区域的基因、种系发生的分析在 25 街 RABV 被执行孤立, CTN 疫苗 7 拉紧代。学习基于 519 bp... 一组 25 个狂犬病病毒(RABV ) ,从 24 条狗和一个人的盒子恢复了,在在 2004 和 2006 之间的中国从各种各样的区域被收集。G-L intergenic 区域的基因、种系发生的分析在 25 街 RABV 被执行孤立, CTN 疫苗 7 拉紧代。学习基于 519 bp 核苷酸顺序的比较,包含 G-L intergenic 区域。中国街紧张的核苷酸顺序相同从 95.5% ~ 100% 。种系发生的分析证明中国的所有孤立清楚地在 Lyssavirus 遗传型 1 支持了所有中国病毒的放置,他们根据他们的地理起源是分布式的。所有仔细中国紧张被联系,但是他们能仍然被划分成二个组:一些街紧张和一些 CTN 紧张。这研究基于 G-L Intergenic 区域的序列关于狂犬病病毒的分子的传染病学介绍细节。关键词狂犬病病毒 - 分子的传染病学 - G-L intergenic 区域 - 中国 CLC 数字 R373.33 基础条款:第 10 国家 five-year-plan (2004BA718 b03 ) 的关键技术 R&D 展开更多
关键词 Rabies virus Molecular Epidemiology G-l intergenic region china
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Multi-fractal Behaviors of Relative Humidity over China 被引量:2
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作者 GAO Li-Hao FU Zun-Tao 《Atmospheric and Oceanic Science Letters》 CSCD 2013年第2期74-78,共5页
The multi-fractal ity over China are studied behaviors of relative humid using the multi-fractal de trended fluctuation analysis (DFA) method. Three multi fractal parameters (the spectrum width Aa, the asymmetry Aa... The multi-fractal ity over China are studied behaviors of relative humid using the multi-fractal de trended fluctuation analysis (DFA) method. Three multi fractal parameters (the spectrum width Aa, the asymmetry Aaas, and the long-range correlation exponent a0) of the singularity spectrum are introduced to quantify the multi-fractal behaviors. The results show that multi-frac tality exists in daily humidity records over most stations in China and is mainly due to the broad distribution of the probability density of the sequence values. Strong multi fractal behaviors over some stations in the Yunnan, Guangdong, and Inner Mongolia provinces are obvious. These behaviors are mainly caused by different long range correlations between large and small fluctuations. The asymmetry of the singularity of relative humidity records is weak, except for a small number of stations in the far east and west of China, where the singularity spec trum is left-skewed. Finally, the long-range correlations in North China are stronger than those in South China, which indicates better predictability in North China. By studying the parameters of the multi-fractal spectrum, various data of long-range power law correlations of the relative humidity records are obtained, which may pro vide theoretical support for climate prediction. 展开更多
关键词 long-range correlation scaling exponent MUlTI-FRACTAl multi-fractal detrended fluctuation analysisCitation: Gao l.-H. and Z.-T. Fu 2013: Multi-fractalbehaviors of relative humidity over china Atmos. Oce-anic Sci. lett. 6 74-78.
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Conodont Biostratigraphy and Age Determination of the Lower-Middle Triassic Boundary in South Guizhou Province,China 被引量:1
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作者 YAO Jianxin JI Zhansheng +6 位作者 WANG Liting WANG Yanbin WU Zhenjie LIU Dunyi WU Guichun ZHANG Jianwei LI Suping 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2011年第2期408-420,共13页
The demarcation of the Lower-Middle Triassic boundary is a disputed problem in global stratigraphic research. Lower-Middle Triassic strata of different types, from platform to basin facies,are well developed in Southw... The demarcation of the Lower-Middle Triassic boundary is a disputed problem in global stratigraphic research. Lower-Middle Triassic strata of different types, from platform to basin facies,are well developed in Southwest China. This is favorable for the study of the Olenekian-Anisian boundary and establishing a stratotype for the Qingyan Stage. Based on research at the Ganheqiao section in Wangmo county and the Qingyan section in Guiyang city, Guizhou province, six conodont zones have been recognized, which can be correlated with those in other regions, in ascending order as follows: 1, Neospathodus cristagalli Interval-Zone; 2, Neospathodus pakistanensis Interval-Zone; 3,Neospathodus waageni Interval-Zone; 4, Neospathodus homeri-N, triangularis Assemblage-Zone; 5,Chiosella timorensis Interval-Zone; and 6, Neogongdolella regalis Range-Zone. An evolutionary series of the Early-Middle Triassic conodont genera Neospathodus-Chiosella-Neogongdolella discovered in the Ganheqiao and Qingyan sections has an intermediate type named Neospathodus qingyanensis that appears between Neospathodus homeri and Chiosella timorensis in the upper part of the Neospathodus homeri-N, triangularis Zone, showing an excellent evolutionary relationship of conodonts near the Lower-Middle Triassic boundary. The Lower-Middle Triassic boundary is located at 1.5 m below the top of the Ziyun Formation, where Chiosella timorensis Zone first appears in the Qingyan section,whereas this boundary is located 0.5 m below the top of the Ziyun Formation, where Chiosella timorensis Zone first appears in the Ganheqiao section. There exists one nearly 6-m thick vitric tuff bed at the bottom of the Xinyuan Formation in the Ganheqiao section, which is usually regarded as a lithologic symbol of the Lower-Middle Triassic boundary in South China. Based on the analysis of high -precision and high-sensitivity Secondary Ion Mass Spectrum data ,the zircon age of this tuff has a weighted mean 206Pb/238U age of 239.0±2.9 Ma (2σ), which is a directly measured zircon U-Pb age of the Lower-Middle Triassic boundary. The Ganheqiao section in Wangmo county can therefore provide an excellent section through the Lower-Middle Triassic because it is continuous, the evolution of the conodonts is distinctive and the regionally stable distributed vitric tuff near the Lower-Middle Triassic boundary can be regarded as a regional key isochronal layer. This section can be regarded not only as a standard section for the establishment of the Qingyan Stage in China, but also as a reference section for the GSSP of the Lower-Middle Triassic boundary. 展开更多
关键词 l-M Triassic Qingyan Stage CONODONT BIOSTRATIGRAPHY isotope chronology South china
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Virescentia guangxiensis(Batrachospermales,Rhodophyta):a new freshwater red algal species from South China
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作者 Kunpeng FANG Fangru NAN +4 位作者 Jia FENG Junping LÜ Qi LIU Xudong LIU Shulian XIE 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2021年第4期1538-1546,共9页
Virescentia guangxiensis,a new species of Virescentia from Guangxi,South China,is described and illustrated based on morphological observations and phylogenetic analysis.This species was distinguished morphologically ... Virescentia guangxiensis,a new species of Virescentia from Guangxi,South China,is described and illustrated based on morphological observations and phylogenetic analysis.This species was distinguished morphologically from other species by the presence of special expansion cells with a variable shape,obovoid,spherical,pear-shaped,located in the penultimate cells of primary or secondary fascicles,rarely terminal on primary fascicles,as well as by small whorls(250-350-μm wide)and short primary fascicles(5-7 cell stories).Phylogenetic analysis of molecular data from the rbc L and COI-5P loci supported the separation of the proposed new species from other species in the genus Virescentia.This is the first species of the order Batrachospermales reported in Guangxi and the second species of the genus Virescentia reported in China.This study expands the known species diversity and geographical distribution of freshwater Rhodophyta in China. 展开更多
关键词 Virescentia new species rbc l COI-5P china
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China vs. EU: Comparison of Int'l Environment
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作者 Qiu Yuanlun Qiu Yuanlun is a Research Professor and former Director at the Institute of European Studies, the Chinese Academy of Social Sciences. 《Contemporary International Relations》 2003年第1期16-19,共4页
When we study the international environment that faces a nation or a region in a specific period, we have to observe its strategic objectives first. China’s strategic objectives in the first two decades of the 21st c... When we study the international environment that faces a nation or a region in a specific period, we have to observe its strategic objectives first. China’s strategic objectives in the first two decades of the 21st century are: To build a well-off society in an all-round way, to achieve complete reunification of the motherland, and to 展开更多
关键词 of HAVE it EU china vs Comparison of Int’l Environment
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China starts a genome-sequencing project of codling moth,Cydia pomonella L.
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《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第1期124-124,共1页
The codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards... The codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), is a destructive insect pest in the fruit production. The emergences of this notorious pest frequently cause the huge economic loss of apple orchards. It can also attacks pears, walnuts and other tree fruits. The codling moth is an important invasive species in China, which first appeared in Kuerle, Xijiang in 1953 and rapidly expanded in the whole area, in 1987 the codling moth spread to Dunhuang, Gansu. Recently, researchers from the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Nanjing Agricultural University and so on initiated a genome-sequencing project of the codling moth. The genome size of the codling moth is 650 Mb, as estimated by flow cytometry and survey sequencing. Since the heterozygousity is a potential obstacle in sequencing the insect genome, the research consortium carefully measured the heterozygousity of the codling moth by SNP analysis and 17-mer estimation, showing that the heterozygousity of the codling moth is around 0.3-0.6%. The whole genome shotgun strategy is adopted and a sequencing plan has been made and officially started. The availability of the codling moth genome should be of great value to uncover the molecular invasion mechanism, high adaptive ability to the stress and to develop efficient control strategies of this pest worldwide and in China. 展开更多
关键词 china starts a genome-sequencing project of codling moth Cydia pomonella l
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Pauciramus yunnanensis,gen.et sp.nov.,a novel freshwater red alga from China with proposal of the Ottiales ord.nov.(Nemaliophycidae,Rhodophyta)
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作者 Kunpeng FANG Fangru NAN +4 位作者 Jia FENG Junping LÜ Qi LIU Xudong LIU Shulian XIE 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第3期1245-1256,共12页
Species of the red algal order Acrochaetiales mostly inhabit marine environments,with only two freshwater taxa Audouinella and Ottia.A new genus and species are described for freshwater red alga Pauciramus yunnanensis... Species of the red algal order Acrochaetiales mostly inhabit marine environments,with only two freshwater taxa Audouinella and Ottia.A new genus and species are described for freshwater red alga Pauciramus yunnanensis from Ailao Mountain,Yunnan,China.It is closely related to Ottia and a new order Ottiales was proposed for these genera.Pauciramus has unique combination of morphological characteristics including the following:plants caespitose and densely pulvinate,slender uniseriate filaments with well-developed rhizoids,rarely branched,cylindrical vegetative cell with a single,ribbon-shaped and parietal chloroplast,reproduction by tetrasporangia,and dense sporangial branchlet only at the upper portion of filaments.Phylogenetic analysis of sequence data from the plastid ribulose-1,5-bisphosphate carboxylase-oxygenase large-subunit(rbc L),small subunit gene of the ribosomal cistron(SSU)and barcode region near the 5′end of the mitochondrial cytochrome oxidase subunit I(COI-5P)indicated that:the new taxon,P.yunnanensis,was in a well-supported clade with Ottia meiospora,and this clade was sister to order Palmariales and Acrochaetiales.To adhere to the principle of monophyly,a new freshwater order Ottiales including Ottia and Pauciramus is proposed.Despite the high sequence interspecific divergences and obvious morphological differences between genera Ottia and Pauciramus,seems impractical to establish a new family for a monospecific genus.Therefore,we temporarily classified Pauciramus into the family Ottiaceae,and made necessary revisions to the description to accommodate this genus. 展开更多
关键词 china 5′end of the mitochondrial cytochrome oxidase subunit I(COI-5P) Ottiales Pauciramus ribulose-1 5-bisphosphate carboxylase-oxygenase large-subunit(rbc l) small subunit gene of the ribosomal cistron(SSU)
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Alibaba’s Tmall and L’Oreal China Partner Up to Launch New Campaign for Men’s Grooming Products
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《China Detergent & Cosmetics》 2018年第4期6-6,共1页
Alibaba Group’s Tmall and L’Oréal China has team up to leverage data-driven consumer analytics and a new value chain that better connects consumers, products and channels. Based on the findings of the latest wh... Alibaba Group’s Tmall and L’Oréal China has team up to leverage data-driven consumer analytics and a new value chain that better connects consumers, products and channels. Based on the findings of the latest white paper codeveloped by Tmall Innovation Center (TMIC) and L’Oréal China Consumer Intelligence Team, the partnership’s first initiative will focus on China’s male-grooming industry. 展开更多
关键词 AlIBABA Group’s Tmall l’Oréal china
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Oriental Contest Ground for Acrobats from the World──Sidelight on 7th China Wuqiao Int'l Art Festival
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《China & The World Cultural Exchange》 2000年第1期6-7,共2页
关键词 Oriental Contest Ground for Acrobats from the World Sidelight on 7th china Wuqiao Int’l Art Festival
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RP-HPLC同时测定菝葜中山柰酚和槲皮素的含量 被引量:5
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作者 舒孝顺 高中洪 杨祥良 《中国药学杂志》 CAS CSCD 北大核心 2006年第6期454-456,共3页
目的 建立一种RP-HPLC测定菝葜山柰酚和槲皮素含量的方法,并测定其不同产地的含量。方法 采用LiehrospherC18柱,水-乙腈-磷酸-三乙胺(65:35:0.27:0.45)作流动相,流速0.7mL·min^-1,二级管阵列检测器(DVD),检测波长36... 目的 建立一种RP-HPLC测定菝葜山柰酚和槲皮素含量的方法,并测定其不同产地的含量。方法 采用LiehrospherC18柱,水-乙腈-磷酸-三乙胺(65:35:0.27:0.45)作流动相,流速0.7mL·min^-1,二级管阵列检测器(DVD),检测波长365nm。结果 槲皮素,山柰酚与相邻杂质能很好分离,其分离度分别为1.96和5.32,理论塔板数以槲皮素峰和山柰酚峰计算,分别为5121和6673;在5~100mg·L^-1内峰面积与其浓度均呈良好的线性关系(r=0.9994,0.9996),样品回收率分别为97.58%和105.67%;两成分含量以湖南张家界的最高而湖北赤壁最低。结论 该方法精密度、重现性和分离效果好,分析快速准确,适合于该药材及提取物中山柰酚和槲皮素含量的测定,不同产地两成分均存在一定的差别。 展开更多
关键词 反相高效液相色谱法 菝葜 槲皮素 山柰酚
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RP-HPLC同时测定菝葜药材中总槲皮素和山柰酚含量 被引量:6
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作者 颜彦 李文霞 叶晓川 《药物分析杂志》 CAS CSCD 北大核心 2008年第2期288-290,共3页
目的:建立同时测定菝葜药材中槲皮素和山柰酚含量的反相高效液相色谱法。方法:色谱柱:Lichrospher C_(18)柱(250 mm×4.6 mm,5 μm),流动相:甲醇-0.5%磷酸水溶液(55:45),流速:1.0 mL·min^(-1),检测波长:365 nm。结果:槲皮素在2... 目的:建立同时测定菝葜药材中槲皮素和山柰酚含量的反相高效液相色谱法。方法:色谱柱:Lichrospher C_(18)柱(250 mm×4.6 mm,5 μm),流动相:甲醇-0.5%磷酸水溶液(55:45),流速:1.0 mL·min^(-1),检测波长:365 nm。结果:槲皮素在2.97~29.7μg·mL^(-1)浓度范围内线性关系良好(r=0.9999),山柰酚在3.01—30.1μg·mL^(-1)浓度范围内线性关系良好(r=0.9999),方法回收率分别为98.9%和99.7%。结论:本方法简便、准确,灵敏度高,重复性好,可为评价菝葜药材质量提供依据。 展开更多
关键词 菝葜 槲皮素 山柰酚 RP—HPlC
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银屑冲剂中原儿茶酸的HPLC法测定 被引量:1
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作者 徐长根 杨海燕 傅强 《药物分析杂志》 CAS CSCD 北大核心 2006年第3期381-383,共3页
目的:建立银屑冲剂中原儿茶酸的含量测定方法。方法:采用反相高效液相色谱法,色谱柱:C_(18)(250 mm×4.6 mm,5 μm);流动相:甲醇-水-冰醋酸(15:85:0.5);流速:1.0 mL.min^(-1);检测波长:260 nm。结果:原儿茶酸在0.1032~0.516μg范... 目的:建立银屑冲剂中原儿茶酸的含量测定方法。方法:采用反相高效液相色谱法,色谱柱:C_(18)(250 mm×4.6 mm,5 μm);流动相:甲醇-水-冰醋酸(15:85:0.5);流速:1.0 mL.min^(-1);检测波长:260 nm。结果:原儿茶酸在0.1032~0.516μg范围内呈良好线性关系,r=0.99996,平均回收率97.27%,RSD为1.4%。结论:建立的方法简便可行,重现性好,提高了银屑冲剂质量控制方法。 展开更多
关键词 银屑冲剂 高效液相色谱法 土茯苓 拔葜 原儿茶酸
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RP-HPLC同时测定菝葜中落新妇苷和黄杞苷的含量 被引量:5
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作者 周艳林 钟小清 +1 位作者 吕高荣 邹节明 《中国药学杂志》 CAS CSCD 北大核心 2008年第14期1103-1105,共3页
目的建立同时测定菝葜药材中落新妇苷和黄杞苷含量的HPLC方法。方法采用Agilent Extend-C_(18)(4.6 mm×250 mm,5μm)柱,以甲醇-水-磷酸溶液(35:65:0.1)为流动相,流速1.0 mL·min^(-1),柱温35℃,检测波长为290nm。结果落新妇苷... 目的建立同时测定菝葜药材中落新妇苷和黄杞苷含量的HPLC方法。方法采用Agilent Extend-C_(18)(4.6 mm×250 mm,5μm)柱,以甲醇-水-磷酸溶液(35:65:0.1)为流动相,流速1.0 mL·min^(-1),柱温35℃,检测波长为290nm。结果落新妇苷和黄杞苷分别在2.16×10^(-3)~1.08,3.176×10^(-3)~1.588μg内呈良好的线性关系;精密度试验RSD分别为0.75%,0.24%;重复性试验RSD分别为0.79%,0.89%;平均加样回收率分别为100.90%,98.71%。结论建立的方法可用于菝葜药材的质量控制。 展开更多
关键词 菝葜 落新妇苷 黄杞苷 高效液相色谱法
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