To investigate the role of signaling pathway in the effect of endoplasmic reticulum stress（ER stress） in endothelial cells stimulated with cigarette smoke extract（CSE）. Human umbilical vein endothelial cells（HUV...To investigate the role of signaling pathway in the effect of endoplasmic reticulum stress（ER stress） in endothelial cells stimulated with cigarette smoke extract（CSE）. Human umbilical vein endothelial cells（HUVECs） were cultured and divided into 3 groups： CSE-stimulated group, CSE-stimulated with 4-PBA group, and negative control group. HUVECs were cultured and stimulated with CSE at concentrations of 5%, 10% and 20%, respectively, mR NA of CXCL-8 and GRP78 was detected by real-time PCR. ELISA was performed to test the expression of CXCL-8 protein, and neutrophils migration was detected by Transwell board test. The NF-κB, ERK, p38 MAPK and transforming growth factor beta（TGF-β） were detected by flow cytometry. The mRNA of CXCL-8 and GRP78 increased in CSE-stimulated HUVECs（P〈0.05）. Furthermore, it was concentration-dependent. 4-PBA significantly reduced the expression of CXCL-8 protein（P〈0.05） and neutrophil migration（P〈0.05）. The TGF-β, rather than the NF-κB, ERK and P38 MAPK pathway might be involved in ER stress stimulated by CSE. CSE induced neutrophils migration by increasing the expression of CXCL-8 in endothelial cells. ER stress might play a role in the effect of neutrophils migration stimulated with CSE, and TGF-β pathway may contribute to the ER stress in HUVECs.展开更多
为保证对船舶动力舱极早期火灾探测的及时性、准确性和可靠性,需要对烟雾浓度检测原理进行改进和烟雾浓度算法的创新。基于电容式检测元胞结构对烟雾浓度检测原理进行全新设计,并采用多尺度烟雾粒子浓度检测算法对检测信号进行处理,以...为保证对船舶动力舱极早期火灾探测的及时性、准确性和可靠性,需要对烟雾浓度检测原理进行改进和烟雾浓度算法的创新。基于电容式检测元胞结构对烟雾浓度检测原理进行全新设计,并采用多尺度烟雾粒子浓度检测算法对检测信号进行处理,以计算出烟雾粒子浓度。试验结果表明:新设计的探测器可实现对0~10%obs/m浓度烟雾粒子的有效检测,检测精度高于百万分比浓度(Parts Per Million,PPM)级;探测器的灵敏度可以达到PPM级;在存在一定浓度的油气和不同粒径灰尘的使用环境中,仍能实现高于PPM级精度的烟雾粒子浓度检测。展开更多
Lung cancer is the leading cause of cancer death in men and women worldwide,with over a million deaths annually.Tobacco smoke is the major etiologic risk factor for lung cancer in current or previous smokers and has b...Lung cancer is the leading cause of cancer death in men and women worldwide,with over a million deaths annually.Tobacco smoke is the major etiologic risk factor for lung cancer in current or previous smokers and has been strongly related to certain types of lung cancer,such as small cell lung carcinoma and squamous cell lung carcinoma.In recent years,there has been an increased incidence of lung adenocarcinoma.This change is strongly associated with changes in smoking behavior and cigarette design.Carcinogens present in tobacco products and their intermediate metabolites can activate multiple signaling pathways that contribute to lung cancer carcinogenesis.In this review,we summarize the smoking-activated signaling pathways involved in lung cancer.展开更多
基金supported by the Natural Science Foundation of Hubei Province,China(No.2017CFB765)
文摘To investigate the role of signaling pathway in the effect of endoplasmic reticulum stress（ER stress） in endothelial cells stimulated with cigarette smoke extract（CSE）. Human umbilical vein endothelial cells（HUVECs） were cultured and divided into 3 groups： CSE-stimulated group, CSE-stimulated with 4-PBA group, and negative control group. HUVECs were cultured and stimulated with CSE at concentrations of 5%, 10% and 20%, respectively, mR NA of CXCL-8 and GRP78 was detected by real-time PCR. ELISA was performed to test the expression of CXCL-8 protein, and neutrophils migration was detected by Transwell board test. The NF-κB, ERK, p38 MAPK and transforming growth factor beta（TGF-β） were detected by flow cytometry. The mRNA of CXCL-8 and GRP78 increased in CSE-stimulated HUVECs（P〈0.05）. Furthermore, it was concentration-dependent. 4-PBA significantly reduced the expression of CXCL-8 protein（P〈0.05） and neutrophil migration（P〈0.05）. The TGF-β, rather than the NF-κB, ERK and P38 MAPK pathway might be involved in ER stress stimulated by CSE. CSE induced neutrophils migration by increasing the expression of CXCL-8 in endothelial cells. ER stress might play a role in the effect of neutrophils migration stimulated with CSE, and TGF-β pathway may contribute to the ER stress in HUVECs.
文摘为保证对船舶动力舱极早期火灾探测的及时性、准确性和可靠性,需要对烟雾浓度检测原理进行改进和烟雾浓度算法的创新。基于电容式检测元胞结构对烟雾浓度检测原理进行全新设计,并采用多尺度烟雾粒子浓度检测算法对检测信号进行处理,以计算出烟雾粒子浓度。试验结果表明:新设计的探测器可实现对0~10%obs/m浓度烟雾粒子的有效检测,检测精度高于百万分比浓度(Parts Per Million,PPM)级;探测器的灵敏度可以达到PPM级;在存在一定浓度的油气和不同粒径灰尘的使用环境中,仍能实现高于PPM级精度的烟雾粒子浓度检测。
基金supported by a fellowship from China Education Council
文摘Lung cancer is the leading cause of cancer death in men and women worldwide,with over a million deaths annually.Tobacco smoke is the major etiologic risk factor for lung cancer in current or previous smokers and has been strongly related to certain types of lung cancer,such as small cell lung carcinoma and squamous cell lung carcinoma.In recent years,there has been an increased incidence of lung adenocarcinoma.This change is strongly associated with changes in smoking behavior and cigarette design.Carcinogens present in tobacco products and their intermediate metabolites can activate multiple signaling pathways that contribute to lung cancer carcinogenesis.In this review,we summarize the smoking-activated signaling pathways involved in lung cancer.
