In this paper,we reviewed the classification,genetic diversity and planting and application of snap bean(Phaseolus vulgaris)germplasm resources in China at the present stage were,analyzed the bottleneck of snap bean r...In this paper,we reviewed the classification,genetic diversity and planting and application of snap bean(Phaseolus vulgaris)germplasm resources in China at the present stage were,analyzed the bottleneck of snap bean research in China,and prospected the development trend of snap bean germplasm research in the future.In the opportunity of the completion of the whole genome sequencing of kidney bean and the construction of high-density genetic map,it is necessary to strengthen the application of ISSR,SNP and other molecular methods and high-throughput proteomics to the study on the specific breeding of snap bean.展开更多
With Fusarium oxysporum f. sp. phaseoli as the target bacterium, the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test....With Fusarium oxysporum f. sp. phaseoli as the target bacterium, the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. This strain was identified according to morphological observation,physiological and biochemical characteristics and sequence analysis of 16 S r RNA gene and gyr B gene. The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against F. oxysporum in snap bean was 71.42% in pot test.展开更多
The effects of arbuscular mycorrhizal fungi(AMF) Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean(Phaseolus vulgaris L.cv Guangzhouyuan) were investigated.Exposure was conducted in g...The effects of arbuscular mycorrhizal fungi(AMF) Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean(Phaseolus vulgaris L.cv Guangzhouyuan) were investigated.Exposure was conducted in growth chambers by using three O3 concentrations(20(CF),80(CFO1) and 120 nL/L(CFO2);8 hr/day for 75 days).Results showed that elevated O3 slightly impacted overall mycorrhizal colonization,but significantly decreased the proportional frequency of hypha and increased the proportional frequency of spores and vesicles,suggesting that O3 had significant effects on mycorrhizal structure.Elevated O3 significantly decreased yield,dry mass and nutrient contents(N,P,K,Ca and Mg) in both non-mycorrhizal and mycorrhizal plants.However,significant interactive effects were found in most variables due to that the reduction by O3 in the mycorrhizal plants was less than that in the non-mycorrhizal plants.Additionally,AMF increased the concentrations of N,P,Ca,and Mg in shoot and root.It can be concluded that AMF alleviated detrimental effects of increasing O3 on host plant through improving plant nutrition and growth.展开更多
目的研究生长期内不同品种油豆角黄酮含量的变化,对金冠豆角种子黄酮种类进行鉴定,并探究发酵处理对金冠种粒中黄酮含量的影响。方法采用乙醇回流法提取7种东北油豆角总黄酮,通过芦丁比色法检测油豆角种粒及荚皮中的黄酮含量,利用超高...目的研究生长期内不同品种油豆角黄酮含量的变化,对金冠豆角种子黄酮种类进行鉴定,并探究发酵处理对金冠种粒中黄酮含量的影响。方法采用乙醇回流法提取7种东北油豆角总黄酮,通过芦丁比色法检测油豆角种粒及荚皮中的黄酮含量,利用超高效液相色谱-四极杆-飞行时间串联质谱法(ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry,UPLC-Q-TOF-MS)检测豆角中黄酮的种类,最后利用唾液链球菌嗜热亚种和植物乳植杆菌L3对金冠豆粒进行发酵处理,探究发酵处理对金冠豆粒黄酮含量的影响。结果油豆角中黄酮含量随着生长时期的增加呈现出先上升后下降的变化过程,金冠油豆角粗黄酮中存在白杨素、黄芩黄素、二氢木蝴蝶素A等11种黄酮类物质,发酵处理均会导致金冠种粒的黄酮含量下降。结论不同品种的油豆角在生长期内黄酮含量的整体变化趋势相似,该研究为油豆角品种选育及产品深加工提供了理论指导。展开更多
基金Supported by Jilin Provincial Science and Technology Development Program:International Science and Technology Cooperation(20200801075GH)Jilin Provincial Agricultural Science and Technology Innovation Project(CXGC2021ZY101)。
文摘In this paper,we reviewed the classification,genetic diversity and planting and application of snap bean(Phaseolus vulgaris)germplasm resources in China at the present stage were,analyzed the bottleneck of snap bean research in China,and prospected the development trend of snap bean germplasm research in the future.In the opportunity of the completion of the whole genome sequencing of kidney bean and the construction of high-density genetic map,it is necessary to strengthen the application of ISSR,SNP and other molecular methods and high-throughput proteomics to the study on the specific breeding of snap bean.
文摘With Fusarium oxysporum f. sp. phaseoli as the target bacterium, the bacterial strain with strong antagonistic activity was screened from crop rhizosphere soil using plate confrontation method and pot efficiency test. This strain was identified according to morphological observation,physiological and biochemical characteristics and sequence analysis of 16 S r RNA gene and gyr B gene. The results showed that LZF-5-3 strain was identified as Bacillus methylotrophicus and the control effect of LZF-5-3 against F. oxysporum in snap bean was 71.42% in pot test.
基金supported by the National Natural Science Foundation of China(No.40701180,30670387)the Chinese Universities Scientific Fund(No.ZZ1106, ZD0904)the Project of Beijing Key Discipline and State Key Laboratory of Urban and Regional Ecology
文摘The effects of arbuscular mycorrhizal fungi(AMF) Glomus mosseae on the responses to elevated O3 in growth and nutrition of snap bean(Phaseolus vulgaris L.cv Guangzhouyuan) were investigated.Exposure was conducted in growth chambers by using three O3 concentrations(20(CF),80(CFO1) and 120 nL/L(CFO2);8 hr/day for 75 days).Results showed that elevated O3 slightly impacted overall mycorrhizal colonization,but significantly decreased the proportional frequency of hypha and increased the proportional frequency of spores and vesicles,suggesting that O3 had significant effects on mycorrhizal structure.Elevated O3 significantly decreased yield,dry mass and nutrient contents(N,P,K,Ca and Mg) in both non-mycorrhizal and mycorrhizal plants.However,significant interactive effects were found in most variables due to that the reduction by O3 in the mycorrhizal plants was less than that in the non-mycorrhizal plants.Additionally,AMF increased the concentrations of N,P,Ca,and Mg in shoot and root.It can be concluded that AMF alleviated detrimental effects of increasing O3 on host plant through improving plant nutrition and growth.
文摘目的研究生长期内不同品种油豆角黄酮含量的变化,对金冠豆角种子黄酮种类进行鉴定,并探究发酵处理对金冠种粒中黄酮含量的影响。方法采用乙醇回流法提取7种东北油豆角总黄酮,通过芦丁比色法检测油豆角种粒及荚皮中的黄酮含量,利用超高效液相色谱-四极杆-飞行时间串联质谱法(ultra performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry,UPLC-Q-TOF-MS)检测豆角中黄酮的种类,最后利用唾液链球菌嗜热亚种和植物乳植杆菌L3对金冠豆粒进行发酵处理,探究发酵处理对金冠豆粒黄酮含量的影响。结果油豆角中黄酮含量随着生长时期的增加呈现出先上升后下降的变化过程,金冠油豆角粗黄酮中存在白杨素、黄芩黄素、二氢木蝴蝶素A等11种黄酮类物质,发酵处理均会导致金冠种粒的黄酮含量下降。结论不同品种的油豆角在生长期内黄酮含量的整体变化趋势相似,该研究为油豆角品种选育及产品深加工提供了理论指导。
