Integrated ribosome and proteome analyses reveal insights into sevoflurane-induced long-term social behavior and cognitive dysfunctions through ADNP inhibition in neonatal mice

A growing number of studies have demonstrated that repeated exposure to sevoflurane during development results in persistent social abnormalities and cognitive impairment.Davunetide,an active fragment of the activity-dependent neuroprotective protein(ADNP),has been implicated in social and cognitive protection.However,the potential of davunetide to attenuate social deficits following sevoflurane exposure and the underlying developmental mechanisms remain poorly understood.In this study,ribosome and proteome profiles were analyzed to investigate the molecular basis of sevoflurane-induced social deficits in neonatal mice.The neuropathological basis was also explored using Golgi staining,morphological analysis,western blotting,electrophysiological analysis,and behavioral analysis.Results indicated that ADNP was significantly down-regulated following developmental exposure to sevoflurane.In adulthood,anterior cingulate cortex(ACC)neurons exposed to sevoflurane exhibited a decrease in dendrite number,total dendrite length,and spine density.Furthermore,the expression levels of Homer,PSD95,synaptophysin,and vglut2 were significantly reduced in the sevoflurane group.Patch-clamp recordings indicated reductions in both the frequency and amplitude of miniature excitatory postsynaptic currents(mEPSCs).Notably,davunetide significantly ameliorated the synaptic defects,social behavior deficits,and cognitive impairments induced by sevoflurane.Mechanistic analysis revealed that loss of ADNP led to dysregulation of Ca^(2+)activity via the Wnt/β-catenin signaling,resulting in decreased expression of synaptic proteins.Suppression of Wnt signaling was restored in the davunetide-treated group.Thus,ADNP was identified as a promising therapeutic target for the prevention and treatment of neurodevelopmental toxicity caused by general anesthetics.This study provides important insights into the mechanisms underlying social and cognitive disturbances caused by sevoflurane exposure in neonatal mice and elucidates the regulatory pathways involved.

A Study on the“Social Behavior”of Zen Poetry Culture:A Case Study of the Sui and Tang Dynasties

During the Sui and Tang dynasties,Buddhism and Zen poetry underwent great development.Zen poetry is diverse in form,strong in personality,and high in achievement.Among the types of Zen poetry are poems,lyrics,and prose,some of which have social attributes closely related to the development of Chinese Buddhism.An analysis reveals that the viewpoints and information conveyed in these poems,lyrics,and prose are positively correlated with the attitudes of the emperors towards Buddhism.When the attitude of the emperor towards Buddhism reached its peak of positivity during the Tang dynasty,there was a change towards a negative correlation with the viewpoints expressed in poetry.

Sex differences in morphine-induced behavioral sensitization and social behaviors in ICR mice

Gender and genetic strain are two prominent variants that influence drug abuse. Although certain sexrelated behavioral responses have been previously characterized in ICR mice, little is known about the effects of sex on morphine-induced behavioral responses in this outbred strain. Therefore, in this study, we investigated the sex differences of morphine-induced locomotion, anxiety-like and social behaviors in ICR mice. After morphine or saline exposure for four consecutive days（twice daily）, increased locomotion, more time spent in the central area, as well as attenuated rearing and self-grooming behaviors were found in morphine-treated females in an open field; no differences were found in locomotion and the time spent in the central area between male and female controls. When interacting with the samesex individuals, female controls were engaged in more social investigation, following, body contacting and self-grooming behaviors than controls; morphine exposure reduced contacting and self-grooming behaviors in females; in contrast, these effects were not found in males. These results indicate that female ICR mice are more prosocial and are more susceptible to morphine exposure than males.

Differences in cocaine-induced place preference persistence, locomotion and social behaviors between C57BL/6J and BALB/cJ mice

C57BL/6J and BALB/cJ mice display significant differences in sociability and response to drugs, but the phenotypic variability of their susceptibility to cocaine is still not well known. In this study, the differences between these two mice strains in the persistence of cocaine-induced conditioned place preference （CPP）, as well as the locomotion and social behaviors after the 24-hour withdrawal from a four-day cocaine （20 mg/kg/day） administration were investigated. The results showed that the cocaine-induced CPP persisted over two weeks in C57BL/6J mice, while it diminished within one week among BALB/cJ mice. After 24-hours of cocaine withdrawal, high levels of locomotion as well as low levels of social interaction and aggressive behavior were found in C57BL/6J mice, but no significant changes were found in BALB/cJ mice, indicating that cocaine-induced CPP persistence, locomotion and social behavior are not consistent between these two strains, and that overall C57BL/6J mice are more susceptible to cocaine than BALB/cJ mice at the tested doses.

Social avoidance behavior in male tree shrews and prosocial behavior in male mice toward unfamiliar conspecifics in the laboratory

Adult male tree shrews vigorously defend against intruding male conspecifics. However, the characteristics of social behavior have not been entirely explored in these males. In this study, male wild-type tree shrews(Tupaia belangeri chinensis)and C57 BL/6 J mice were first allowed to familiarize themselves with an open-field apparatus. The tree shrews exhibited a short duration of movement(moving) in the novel environment, whereas the mice exhibited a long duration of movement. In the 30 min social preference-avoidance test, target animals significantly decreased the time spent by the experimental tree shrews in the social interaction(SI)zone, whereas experimental male mice exhibited the opposite. In addition, experimental tree shrews displayed a significantly longer latency to enter the SI zone in the second 15 min session(targetpresent) than in the first 15 min session(targetabsent), which was different from that found in mice.Distinct behavioral patterns in response to a conspecific male were also observed in male tree shrews and mice in the first, second, and third 5 min periods. Thus, social behaviors in tree shrews and mice appeared to be time dependent. In summary,our study provides results of a modified social preference-avoidance test designed for the assessment of social behavior in tree shrews. Our findings demonstrate the existence of social avoidance behavior in male tree shrews and prosocial behavior in male mice toward unfamiliar conspecifics. The tree shrew may be a new animal model, which differs from mice, for the study of social avoidance and prosocial behaviors.

Stress-relevant social behaviors of middle-class male cynomolgus monkeys （Macaca fascicularis）

Stress from dominance ranks in human societies, or that of other social animals, especially nonhuman primates, can have negative influences on health. Individuals holding different social status may be burdened with various stress levels. The middle class experiences a special stress situation within the dominance hierarchy due to its position between the higher and lower classes. Behaviorally, questions about where middle-class stress comes from and how individuals adapt to middle-class stress remain poorly understood in nonhuman primates. In the present study, social interactions, including aggression, avoidance, grooming and mounting behaviors, between beta males, as well as among group members holding higher or lower social status, were analyzed in captive male-only cynomolgus monkey groups. We found that aggressive tension from the higher hierarchy members was the main origin of stress for middle- class individuals. However, behaviors such as attacking lower hierarchy members immediately after being the recipient of aggression, as well as increased avoidance, grooming and mounting toward both higher and lower hierarchy members helped alleviate middle-class stress and were particular adaptations to middle-class social status.

Spring home range and social behavior of Hazel grouse at Changbai Mountain

春天家范围和榛的社会行为埋怨，(Bonasa bonasia ) 在 1993 鈥 ? 用生物遥测学在 Changbai 山被学习 4。开始在的成群溶解和对形成中间 -- 前进。配对的男性在春天保持了领土，区域从 3.2 hm2 到 17.3 hm2 (n=2 ) 。榛野鸡被证明由生物遥测学工作单配。在配对的 No.10 和 No.8 之间的家范围的重叠是 82.4% 。在一起是的鸟的时间在 prelaying 时期(n=53 ) 期间是 83.0% 时间，证明对契约是稳定的。奇男性显示出不同行为，保持了领土或漫步，并且他们在早 5 月改变了行为。学习建议奇男性放弃了他们的繁殖目标，并且为幸存的获得在早 5 月变得更重要，当女性开始了孵化时。

School Boarding and Students'Prosocial Behaviors:Evidence from Rural China

This study investigated the efects of boarding at school on students'prosocial behaviors in rural China using data from the National Children's Study of China.The instrumental variable(IV)approach was used to control for potential endogeneity,and the IVs were the proportion of boarding students in higher grades and the school area per student.The ordinary least squares and IV estimates showed that boarding students exhibited more prosocial behaviors,including compliance with rules,positive traits,and altruistic attitudes.These results were robust.Heterogeneity analyses suggested that students from low-income families,children who were not"left behind,"high-grade students,and female students were more likely to benefit from boarding.We found that these effects were primarily due to boarding students developing stronger feelings of trust and support from their peers and teachers and participating in more school-organized events and team activities.

Fertility control of Rattus nitidus using quinestrol:effects on reproductive organs and social behavior

Fertility control has been identified by studies in the laboratory and the field as a more appropriate and long-term control strategy for rodent pests than lethal control.In this study,we investigated the effects of quinestrol on mass of reproductive organs and on social behaviors in female and male Himalayan field rats(Rattus nitidus).A total of 16 adult females and 16 adult males were randomly assigned to 4 groups.One male and one female group were fed rice with 0.005%quinestrol by weight for 7 days,and another 2 groups were fed rice only.After 7 days,rats were assigned to 10 min dyadic encounters between groups,and investigation,aggression,defense and attack latency were quantified.All animals were killed on day 10,and reproductive organs were dissected and weighed.Dyadic encounter data showed that there were obvious changes in social behaviors of quinestrol-treated rats.Quinestrol significantly inhibited the investigative behavior of quinestrol-treated males toward control females in Rattus nitidus,but seldom affected investigation between control males and quinestrol-treated females.Aggression of control females toward quinestrol-treated males was higher than that of quinestrol-treated females,and defense of quinestrol-treated males toward control females was more remarkable than that of control males.Quinestrol remarkably decreased wet masses of epididymis and spermotophore in males and ovaries in females,but had no effect on wet masses of testes and uteri after quinestrol treatment.These results indicate that the anti-fertility effects of quinestrol on R.nitidus are attributed to not only suppressing reproductive organs but also impacting social behaviors associated with territory defense and mate choice.

Design,challenges,and the potential of transcriptomics to understand social behavior

Rapid advances in Ribonucleic Acid sequencing(or RNA-seq)technology for analyzing entire transcriptomes of desired tissue samples,or even of single cells at scale,have revolutionized biology in the past decade.Increasing accessibility and falling costs are making it possible to address many problems in biology that were once considered intractable,including the study of various social behaviors.RNA-seq is opening new avenues to understand long-standing questions on the molecular basis of behavioral plasticity and individual variation in the expression of a behavior.As whole transcriptomes are examined,it has become possible to make unbiased discoveries of underlying mechanisms with little or no necessity to predict genes involved in advance.However,researchers need to be aware of technical limitations and have to make specific decisions when applying RNA-seq to study social behavior.Here,we provide a perspective on the applications of RNA-seq and experimental design considerations for behavioral scientists who are unfamiliar with the technology but are considering using it in their research.

The Influence of Hedonistic Culture to Minangkabau Juveniles’Social Behaviors in the Twenty-first Century

This study is based on the emergence of teenagers1 behaviors which is deviated from the tradition of Minangkabau society.It aims to determine how hedonism affects Minangkabau's local pearls of wisdom.A qualitative approach with phenomenology research design was used where 25 Minangkabau teenagers,religious and traditional leaders who live in Lima Puluh Kota,Tanah Datar,and Agam involved in the study.The data taken from observation,interview,and documentation.The results reveal that the paradigm shift of young Minangkabau's social behavior is caused by hedonism that can be seen in both positive and negative aspects.In positive ways,they live their lives mainly to fulfill their needs,add experiences,create a sense of secure for the community,and be open minded.Meanwhile,in negative ways,traditions based on Islamic laws and the Holly Quran were regarded only as symbols.Such hedonism tends to trigger crime,individualism,laziness,free-sex,insecurity,and consumptive.Thus,this kind of behavior was caused by the absence of religious and custom education they had in the family and social lives.

Ant Pupae from Myanmar Amber Reveal the Evolutionary Process of Cocoon-Spinning Behavior in Formicidae

Many extant species spin cocoons before their pupal stage,which can protect them from predators,pathogens,and fluctuant environmental factors in nests.However,owing to the scarcity of ant cocoon fossils,little is known about the origin and evolution of this behavior in Formicidae.Here,we describe two ant pupae specimens from Myanmar amber.The presence of cocoons in these Mesozoic fossils suggests the ancestral origin of spinning cocoon behavior;based on our phylogenetic analysis and ancestral state reconstruction,this behavior was secondarily lost at least six times in Formicidae,which could be ascribed to the transition of nesting and feeding habits,as well as the development of eusociality.Moreover,the first specimen preserved worker ants with empty cocoons simultaneously,indicating garbage removal or brood care behavior,which improves our understanding of their eusociality in the Cretaceous.

Influence of Information Technology on Social Spatial Behaviors of Urban Residents——Case of Nanjing City in China

Taking Nanjing as a case, the paper explains the spatial behavior differences existing in the information technology use among different groups of residents and households, by virtue of analyzing the survey data of urban households in the 11 districts of Nanjing, from the social, spatial, life and other non-technical angles. Also it makes various analyses and evaluation quantitatively and qualitatively on the social and spatial effect of information technology. The results show that the new technology is changing the social spatial behaviors of urban residents. New behavioral spaces of urban family such as telecommuting, email and QQ have begun to emerge. With the help of Internet, the communication scope of families has expanded greatly, and more new forms of publicizing community information have begun to emerge. Telecommunication contact forms have been developing swiftly, and their frequencies of contact have been increasing dramatically.

The Research on E-mail Users' Behavior of Participating in Subjects Based on Social Network Analysis

The e-mail network is a type of social network. This study analyzes user behavior in e-mail subject participation in organizations by using social network analysis. First, the Enron dataset and the position-related information of an employee are introduced, and methods for deletion of false data are presented. Next, the three-layer model(User, Subject, Keyword) is proposed for analysis of user behavior. Then, the proposed keyword selection algorithm based on a greedy approach, and the influence and propagation of an e-mail subject are defined. Finally, the e-mail user behavior is analyzed for the Enron organization. This study has considerable significance in subject recommendation and character recognition.

Social Network Information Propagation Model Based on Individual Behavior

In this paper, we discuss building an information dissemination model based on individual behavior. We analyze the individual behavior related to information dissemination and the factors that affect the sharing behavior of individuals, and we define and quantify these factors. We consider these factors as characteristic attributes and use a Bayesian classifier to classify individuals. Considering the forwarding delay characteristics of information dissemination, we present a random time generation method that simulates the delay of information dissemination. Given time and other constraints, a user might not look at all the information that his/her friends published. Therefore, this paper proposes an algorithm to predict information visibility, i.e., it estimates the probability that an individual will see the information. Based on the classification of individual behavior and combined with our random time generation and information visibility prediction method, we propose an information dissemination model based on individual behavior. The model can be used to predict the scale and speed of information propagation. We use data sets from Sina Weibo to validate and analyze the prediction methods of the individual behavior and information dissemination model based on individual behavior. A previously proposedinformation dissemination model provides the foundation for a subsequent study on the evolution of the network and social network analysis. Predicting the scale and speed of information dissemination can also be used for public opinion monitoring.

Social correlates of sedentary behavior in young people:The UP&DOWN study

Purpose:The aim of the present study was to analyze the associations of youths' sedentary behavior(SB) with parents' and siblings' SB and physical activity(PA),as well as the associations of youths' coparticipation with parents,siblings,and friends in PA and SB with youths' SB.Methods:The sample consisted of 1543 youths(12.02±2.51 years;788 boys) enrolled in the baseline cohort of the UP&DOWN study.SB was assessed by accelerometry and questionnaire.Participants reported the time spent by their parents and siblings watching television,playing videogames,surfing the Internet,sitting/resting,and doing PA.Further,participants reported coparticipation with parents,siblings,and friends in these activities.Linear mixed models,including school and city as random effects,were performed.Results:Parents' television time was positively associated with youths' screen-based SB.Coparticipation with friends in playing videogames(in boys) and in surfing the Internet(in girls) showed a positive association with screen-based SB and a negative association with educational-based SB.Moreover,coparticipation with siblings and friends in PA was inversely associated with accelerometer-based SB in boys and girls.Conclusion:Our results emphasize the important role of social modeling in the development of sedentary lifestyles in youths.Interventions aimed at reducing health risk behaviors in youths could be more effective if they are oriented from a social perspective that involves their families and networks of their closest friends.

Impact of social isolation and resident intruder stress on aggressive behavior in the male rat

Stress studies frequently utilize physical stressors to establish animal models of stress. In the majority of cases, these models are not consistent with human circumstances. The present study simulated a social isolation plus resident intruder stress model in the rat. The rats were subjected to daily social isolation and resident intruder stress for 2 weeks. Behaviors were then tested. Rats subjected to social stress exhibited different aggressive behavior styles; some rats had greater scores on composite aggression behaviors, as well as locomotor and exploratory activity, but lower scores on latency than others. The high-aggressive group exhibited predominantly anger-out, while the low-aggressive group exhibited anger-in. Results suggest that social isolation plus resident intruder may serve as an appropriate model for anger-in and anger-out emotion modeling in the rat.

The Association between Awareness of Sexual Behavior and Cognitive Social Capital among High School Students in Vietnam

Background: Globally, adolescent sexual behavior is a major health concern and the focus of many researches. However, negative attitude towards premarital sex and sense of community belonging have not been examined in relation to adolescent sexual behavior. The purpose of this study was to examine whether these two factors are associated with adolescent sexual behavior. Methods: We conducted a cross-sectional survey of 1672 eleventh grade students from three public high schools in Hanoi, Vietnam. Participants’ sense of community belonging and negative attitude towards premarital sex were assessed through a self-administered questionnaire. We performed multiple logistic regression analyses to examine associations between the cognitive social capital concepts, such as a sense of community belonging. Results: 92.3% of students agreed with a sense of community belonging, but only 14.1% of students accepted premarital sex. A high sense of community belonging was significantly associated with negative attitude towards premarital sex OR (95% CI), male 3.16 (2.3-4.34), female 8.77 (5.38-14.29). After adjusting for a sense of community belonging, the association disappeared between acceptance of premarital sex and sexual experience of peers, proper time to wear a condom, and Rosenberg Self-Esteem Scale score. Conclusion: Cognitive social capital, such as sense of community belonging, was associated with negative attitude towards premarital sex. Therefore, cognitive social capital concepts should be included in sexual behavior and health promotions, especially with adolescents.

Social bookmarking behaviors of college students:A survey of Wuhan University Library users

Purpose:To have a better understanding of library users' social bookmarking behaviors,we conducted a survey of college students to examine how much attention they have paid to social bookmarking results,their social bookmarking motivations and behaviors on book sharing sites and in their library's OPAC,and their expectations to their library's social bookmarking service.Design/methodology/approach:A questionnaire survey was conducted to investigate social bookmarking behaviors and motivations of users of Wuhan University Library(WUL) on book sharing sites and in WUL's OPAC.A total of 700 questionnaires were distributed and357 valid questionnaires were retrieved.SPSS was used for data analysis.Findings:The results revealed that there were differences between users' social bookmarking behaviors on book sharing sites and in the Library's OPAC.Users preferred to use tags than add tags on book sharing sites.The main tagging motivation on book sharing sites was for meeting users' specific needs such as collection management and book recommendations.As for the Library,the lack of publicity and promotion of the social bookmarking service led to insufficient use of the Library's service.Due to a lack of knowledge of the social bookmarking service,users did not care about the lectures or publicity campaigns about the Library's social bookmarking service.Research limitations:Because social bookmarking was not common among WUL users,and the questionnaires could be handed out to a limited number of people,it was hard to describe users' social bookmarking behaviors in the Library's OPAC by investigating a sample of 357Library users,and therefore we only investigated users' social bookmarking behaviors on the book sharing sites.Practical implications:The survey results provide insights into library users' social bookmarking behaviors and motivations.The study will help academic libraries improve their social bookmarking service.Originality/value:In spite of growing popularity of social bookmarking sites,little has been known about the social bookmarking behaviors of library users.This study investigated college students' social bookmarking behaviors and motivations,providing suggestions for academic libraries to improve their social bookmarking services.