To investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia reperfusion in rats Methods Male Sprague Dawley (SD) rats were scheduled ...To investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia reperfusion in rats Methods Male Sprague Dawley (SD) rats were scheduled to undergo 3 hour middle cerebral artery occlusion by intraluminal suture and 24 hour reperfusion Neurologic outcomes were score d on a 0-5 grading scale Infarct volume was shown with triphenyltetrazolium c hloride staining and measured by an image analysis system Concentrations of va rious amino acids (aspartate, glutamate, glycine, taurine, and gama aminobutyri c acid) were measured after 3 hours of reperfusion using high performance liquid chromatography Propofol, midazolam and thiopental sodium were given intraperi toneally at the beginning of reperfusion Results Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes Propofol showed better neurological protection than midazol am while thiopental sodium did not exhibit any protective effect Both propofol and midazolam decreased excitatory amino acids accumulation, while propofol inc reased gama aminobutyric acid accumulation in ischemic areas in reperfusion Conclusion Propofol and midazolam, but not thiopental sodium, may provide protective effect s against reperfusion induced injury in rats subjected to focal cerebral ischemi a This neurological protection may be due to the acceleration of excitatory am ino acids elimination in reperfusion展开更多
文摘To investigate the effects of propofol, midazolam and thiopental sodium on outco mes and amino acid accumulation in focal cerebral ischemia reperfusion in rats Methods Male Sprague Dawley (SD) rats were scheduled to undergo 3 hour middle cerebral artery occlusion by intraluminal suture and 24 hour reperfusion Neurologic outcomes were score d on a 0-5 grading scale Infarct volume was shown with triphenyltetrazolium c hloride staining and measured by an image analysis system Concentrations of va rious amino acids (aspartate, glutamate, glycine, taurine, and gama aminobutyri c acid) were measured after 3 hours of reperfusion using high performance liquid chromatography Propofol, midazolam and thiopental sodium were given intraperi toneally at the beginning of reperfusion Results Both propofol and midazolam attenuated neurological deficits and reduced infarct and edema volumes Propofol showed better neurological protection than midazol am while thiopental sodium did not exhibit any protective effect Both propofol and midazolam decreased excitatory amino acids accumulation, while propofol inc reased gama aminobutyric acid accumulation in ischemic areas in reperfusion Conclusion Propofol and midazolam, but not thiopental sodium, may provide protective effect s against reperfusion induced injury in rats subjected to focal cerebral ischemi a This neurological protection may be due to the acceleration of excitatory am ino acids elimination in reperfusion
