圆裂短足软珊瑚Cladiella krempfi中的甾醇苷

Soft corals yield a wide variety of terepenes, steroids and glycosides. The soft coral Cladiella krempfi has been studied by Christopher, and Bheemasankara and five cembanoid diterpenes have been obtained. In our continuing search to discover the bioactive substances from Chinese soft coral, a species of the soft coral Cladiella krempfi collected from Weizhou Island, Guangxi Province were investigated. Two steroidal glycosides 1 and 2 were first isolated from the ethanol extract. On the basis of the spectroscopic methods their structures were established as 3-O-[-D-arabinopyranosyl]-pregn-5,20-dien-3β-ol(1) and \{4-O-[β-D-arabinopyranosyl]-pregn-20-en-3β,4α-diol(2)\}. Steroidal glycosides were first found from this species of soft coral. Compound 1 was a new compound. Compound 2 was obtained from the South China Sea soft coral, Lobophyton sp. Bioassay showed that compound 2 displayed cytotoxic against human Hep-G2 and Cne2 cell lines in vitro with IC 50 values 27.9, 14.3 μg/mL respectively.

中国南海圆裂短足软珊瑚的甾体成分研究

从南海圆裂短足软珊瑚（Cladiella krempfi）中分离出12个甾体化合物，分别鉴定为：1，2-[1’，3’，4’]oxodithiin-pregna-20-en-3-one（1）；19-nor-4-methylpregna-1，3，5（10），20-tetraen-1-ol（2）；pregna-1-en-3-one-20-01（3）；pregna-1，7-dien-20-ol-3-one（4）；pregna-5，20-dien-3-O-a—fucopyranoside（5）；pregna-20-en-3-O-a—fucopyranoiside（6）；孕甾-1，20-二烯-3-酮（7）；孕甾-1，4，20-三烯-3-酮（8）；24-甲基-5d-胆甾-7，22-二烯-3β，5，6β三醇（9）；26-甲基-24-亚甲基胆甾-5烯-3β-醇（10）；胆甾-5-烯-3β醇（11）；β-谷甾醇（12）。化合物1～6系首次从Cladiella属软珊瑚中获得的孕甾类化合物。对所获得化合物的生源途径进行了初步讨论。