A new ceramide, humesamide, was isolated from the soft coral Cladiella humesi Verseveldt, which was collected from Linshui County of Hainan Province. Its structure was established by spectroscopic analysis and chemica...A new ceramide, humesamide, was isolated from the soft coral Cladiella humesi Verseveldt, which was collected from Linshui County of Hainan Province. Its structure was established by spectroscopic analysis and chemical degradation methods.展开更多
One new furanocembranoid diterpene,11-hydroxy-Δ^(12(13))-pukalide(1),along with six known secondary metabolites,11-acetoxy-Δ^(12(13))-pukalide(2),13α-acetoxypukalide(3),pukalide(4),3α-methoxyfuranocembranoid(5),Δ...One new furanocembranoid diterpene,11-hydroxy-Δ^(12(13))-pukalide(1),along with six known secondary metabolites,11-acetoxy-Δ^(12(13))-pukalide(2),13α-acetoxypukalide(3),pukalide(4),3α-methoxyfuranocembranoid(5),Δ9(15)-africanene(6),and methyl(5′E)-5-(2′,6′-dimethylocta-5′,7′-dienyl)furan-3-carboxylate(7)were isolated from the Okinawan soft coral Sinularia sp.Their chemical structures were elucidated based on spectroscopic analysis(FTIR,NMR,and HRESIMS),and the relative stereochemistry of 1 was determined by NOESY experiments and acetylation,which yielded derivative 2.In addition,compounds 1 and 7 exhibited toxicity in the brine shrimp lethality test.展开更多
Two new steroidal glycosides and one polyhydroxylated sterol were isolated from the soft coral Cladiella sp. by HPLC fractionation. The animal material was collected from Sanya bay, Hainan province, China. On the basi...Two new steroidal glycosides and one polyhydroxylated sterol were isolated from the soft coral Cladiella sp. by HPLC fractionation. The animal material was collected from Sanya bay, Hainan province, China. On the basis of spectroscopic analysis and chemical conversion, their chemical structures were elucidated as pregn-5,20-dien-3β-ol-3-O-α-L-fucopyranoside(1), pregn-5,20-dien-3β-ol-3-O-β-D-xylopyranoside(2) and (22E,24R)-ergost-7,22-dien-3β,5α,6β-triol(3). Compounds 1 and 2 are new compounds.展开更多
Soft corals yield a wide variety of terepenes, steroids and glycosides. The soft coral Cladiella krempfi has been studied by Christopher, and Bheemasankara and five cembanoid diterpenes have been obtained. In our cont...Soft corals yield a wide variety of terepenes, steroids and glycosides. The soft coral Cladiella krempfi has been studied by Christopher, and Bheemasankara and five cembanoid diterpenes have been obtained. In our continuing search to discover the bioactive substances from Chinese soft coral, a species of the soft coral Cladiella krempfi collected from Weizhou Island, Guangxi Province were investigated. Two steroidal glycosides 1 and 2 were first isolated from the ethanol extract. On the basis of the spectroscopic methods their structures were established as 3-O-[-D-arabinopyranosyl]-pregn-5,20-dien-3β-ol(1) and \{4-O-[β-D-arabinopyranosyl]-pregn-20-en-3β,4α-diol(2)\}. Steroidal glycosides were first found from this species of soft coral. Compound 1 was a new compound. Compound 2 was obtained from the South China Sea soft coral, Lobophyton sp. Bioassay showed that compound 2 displayed cytotoxic against human Hep-G2 and Cne2 cell lines in vitro with IC 50 values 27.9, 14.3 μg/mL respectively.展开更多
Penicildiones A-D(1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy(5), stachybotrylactone B(6) and stachybo...Penicildiones A-D(1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy(5), stachybotrylactone B(6) and stachybotrin(7) were isolated from the soft coral-derived fungus Penicillium sp. SCSIO41201, cultured in the 1% NaCl PDB substrate. Their structures were determined through spectroscopic methods and X-ray crystallography. Biological evaluation results revealed that 6 exhibited significant cytotoxic activity against HL-60, K562, MOLT-4, ACHN, 786-O, and OS-RC-2 cell lines with IC50 values of 5.23, 4.12, 4.31, 23.55, 7.65 and 10.81 μmol·L-1, respectively, while other compounds showed weak or no cytotoxicity at 50 μmol·L-1.展开更多
基金This work was supported by the National Science Foundation of China !(960012).
文摘A new ceramide, humesamide, was isolated from the soft coral Cladiella humesi Verseveldt, which was collected from Linshui County of Hainan Province. Its structure was established by spectroscopic analysis and chemical degradation methods.
文摘One new furanocembranoid diterpene,11-hydroxy-Δ^(12(13))-pukalide(1),along with six known secondary metabolites,11-acetoxy-Δ^(12(13))-pukalide(2),13α-acetoxypukalide(3),pukalide(4),3α-methoxyfuranocembranoid(5),Δ9(15)-africanene(6),and methyl(5′E)-5-(2′,6′-dimethylocta-5′,7′-dienyl)furan-3-carboxylate(7)were isolated from the Okinawan soft coral Sinularia sp.Their chemical structures were elucidated based on spectroscopic analysis(FTIR,NMR,and HRESIMS),and the relative stereochemistry of 1 was determined by NOESY experiments and acetylation,which yielded derivative 2.In addition,compounds 1 and 7 exhibited toxicity in the brine shrimp lethality test.
文摘Two new steroidal glycosides and one polyhydroxylated sterol were isolated from the soft coral Cladiella sp. by HPLC fractionation. The animal material was collected from Sanya bay, Hainan province, China. On the basis of spectroscopic analysis and chemical conversion, their chemical structures were elucidated as pregn-5,20-dien-3β-ol-3-O-α-L-fucopyranoside(1), pregn-5,20-dien-3β-ol-3-O-β-D-xylopyranoside(2) and (22E,24R)-ergost-7,22-dien-3β,5α,6β-triol(3). Compounds 1 and 2 are new compounds.
文摘Soft corals yield a wide variety of terepenes, steroids and glycosides. The soft coral Cladiella krempfi has been studied by Christopher, and Bheemasankara and five cembanoid diterpenes have been obtained. In our continuing search to discover the bioactive substances from Chinese soft coral, a species of the soft coral Cladiella krempfi collected from Weizhou Island, Guangxi Province were investigated. Two steroidal glycosides 1 and 2 were first isolated from the ethanol extract. On the basis of the spectroscopic methods their structures were established as 3-O-[-D-arabinopyranosyl]-pregn-5,20-dien-3β-ol(1) and \{4-O-[β-D-arabinopyranosyl]-pregn-20-en-3β,4α-diol(2)\}. Steroidal glycosides were first found from this species of soft coral. Compound 1 was a new compound. Compound 2 was obtained from the South China Sea soft coral, Lobophyton sp. Bioassay showed that compound 2 displayed cytotoxic against human Hep-G2 and Cne2 cell lines in vitro with IC 50 values 27.9, 14.3 μg/mL respectively.
基金the National Natural Science Foundation of China (Nos. 21977102, 81973235,81860626, 21772210, 41776169)Guangzhou Science and Technology Project (No. 201804010462)+3 种基金the Natural Science Foundation of Guangdong Province (No. 2018A0303130219)the Special Funds for Promoting Economic Development (Marine Economic Development)of Guangdong Province (No. GDOE[2019]A28)National Major Scientific and Technological Special Project for Significant New Drugs Development (No. 2018ZX09735001-002-003)Project from Institution of South China Sea Ecology and Environmental Engineering(No. ISEE2018PY04)。
文摘Penicildiones A-D(1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy(5), stachybotrylactone B(6) and stachybotrin(7) were isolated from the soft coral-derived fungus Penicillium sp. SCSIO41201, cultured in the 1% NaCl PDB substrate. Their structures were determined through spectroscopic methods and X-ray crystallography. Biological evaluation results revealed that 6 exhibited significant cytotoxic activity against HL-60, K562, MOLT-4, ACHN, 786-O, and OS-RC-2 cell lines with IC50 values of 5.23, 4.12, 4.31, 23.55, 7.65 and 10.81 μmol·L-1, respectively, while other compounds showed weak or no cytotoxicity at 50 μmol·L-1.
