Microbial Activity in a Temperate Forest Soil as Affected by Elevated Atmospheric CO_2

Microorganisms play a key role in the response of soil ecosystems to the rising atmospheric carbon dioxide （CO2） as they mineralize organic matter and drive nutrient cycling. To assess the effects of elevated CO2 on soil microbial C and N immobilization and on soil enzyme activities, in years 8 （2006） and 9 （2007） of an open-top chamber experiment that begun in spring of 1999, soil was sampled in summer, and microbial biomass and enzyme activity related to the carbon （C）, nitrogen （N） and phosphorus （P） cycling were measured. Although no effects on microbial biomass C were detected, changes in microbial biomass N and metabolic activity involving C, N and P were observed under elevated CO2. Invertase and .dehydrogenase activities were significantly enhanced by different degrees of elevated CO2. Nitrifying enzyme activity was significantly （P 〈 0.01） increased in the August 2006 samples that received the elevated COs treatment, as compared to the samples that received the ambient treatment. Denitrifying enzyme activity was significantly （P 〈 0.04） decreased by elevated COs treatments in the August 2006 and June 2007 （P 〈 0.09） samples, β-N-acetylglucosaminidase activity was increased under elevated CO2 by 7% and 25% in June and August 2006, respectively, compared to those under ambient CO2. The results of June 2006 samples showed that acid phosphatase activity was significantly enhanced under elevated CO2. Overall, these results suggested that elevated CO2 might cause changes in the belowground C, N and P cycling in temperate forest soils.

Dynamic carbon-nitrogen coupling under global change

Carbon-nitrogen coupling is a fundamental principle in ecosystem ecology.However,how the coupling responds to global change has not yet been examined.Through a comprehensive and systematic literature review,we assessed how the dynamics of carbon processes change with increasing nitrogen input and how nitrogen processes change with increasing carbon input under global change.Our review shows that nitrogen input to the ecosystem mostly stimulates plant primary productivity but inconsistently decreases microbial activities or increases soil carbon sequestration,with nitrogen leaching and nitrogenous gas emission rapidly increasing.Nitrogen fixation increases and nitrogen leaching decreases to improve soil nitrogen availability and support plant growth and ecosystem carbon sequestration under elevated CO_(2)and temperature or along ecosystem succession.We conclude that soil nitrogen cycle processes continually adjust to change in response to either overload under nitrogen addition or deficiency under CO_(2)enrichment and ecosystem succession to couple with carbon cycling.Indeed,processes of both carbon and nitrogen cycles continually adjust under global change,leading to dynamic coupling in carbon and nitrogen cycles.The dynamic coupling framework reconciles previous debates on the“uncoupling”or“decoupling”of ecosystem carbon and nitrogen cycles under global change.Ecosystem models failing to simulate these dynamic adjustments cannot simulate carbonnitrogen coupling nor predict ecosystem carbon sequestration well.

Denitrification from nitrogen-fixing biologically crusted soils in a cool desert environment,southeast Utah,USA

Introduction:Nitrogen fixation by microorganisms within biological soil crust(“biocrust”)communities provides an important pathway for N inputs in cool desert environments where soil nutrients are low and symbiotic N-fixing plants may be rare.Estimates of N fixation in biocrusts often greatly exceed that of N accretion rates leading to uncertainty regarding N loss pathways.Methods:In this study we examined nitrogen fixation and denitrification rates in biocrust communities that differed in N fixation potential(low N fixation=light cyanobacterial biocrust,high N fixation=dark cyanolichen crust)at four temperature levels(10,20,30,40°C)and four simulated rainfall levels(0.05,0.2,0.6,1 cm rain events)under controlled laboratory conditions.Results:Acetylene reduction rates(AR,an index of N fixation activity)were over six-fold higher in dark crusts relative to light crusts.Dark biocrusts also exhibited eight-fold higher denitrification rates.There was no consistent effect of temperature on denitrification rates,but there was an interactive effect of water addition and crust type.In light crusts,denitrification rates increased with increasing water addition,whereas the highest denitrification rates in dark crusts were observed at the lowest level of water addition.Conclusions:These results suggest that there are no clear and consistent environmental controls on short-term denitrification rates in these biologically crusted soils.Taken together,estimates of denitrification from light and dark biocrusts constituted 3 and 4%of N fixation rates,respectively suggesting that losses as denitrification are not significant relative to N inputs via fixation.This estimate is based on a previously published conversion ratio of ethylene produced to N fixed that is low(0.295),resulting in high estimates of N fixation.If future N fixation studies in biologically crusted soils show that these ratios are closer to the theoretical 3:1 ratio,denitrification may constitute a more significant loss pathway relative to N fixed.