One type of soil collected from Maoer-shan in Heilongjiang Province,China was selected to induce hyphal growth of Tricholoma matsutake by a soil screening ex-periment.It was confirmed that hyphal growth of all the tes...One type of soil collected from Maoer-shan in Heilongjiang Province,China was selected to induce hyphal growth of Tricholoma matsutake by a soil screening ex-periment.It was confirmed that hyphal growth of all the tested T.matsutake isolates was significantly stimulated in soil by supplemented with 0.5%~2.0%olive oil.The ag-gregation of hyphae and soil resembled natural Shiro.The biomass of hyphae in the soil increases with increasing olive oil concentrations.Moreover,seedlings of Pinus den-siflora grew well in the soil containing 0.5%~1.0%olive oil and were also successfully infected by T.matsutake isolate A in the soil containing 1.0%olive oil.This study estab-lished a culture system of artificial Shiro formation and also provided a premise for formulation of culture substratum for fruit body formation of T.matsutake.展开更多
文摘One type of soil collected from Maoer-shan in Heilongjiang Province,China was selected to induce hyphal growth of Tricholoma matsutake by a soil screening ex-periment.It was confirmed that hyphal growth of all the tested T.matsutake isolates was significantly stimulated in soil by supplemented with 0.5%~2.0%olive oil.The ag-gregation of hyphae and soil resembled natural Shiro.The biomass of hyphae in the soil increases with increasing olive oil concentrations.Moreover,seedlings of Pinus den-siflora grew well in the soil containing 0.5%~1.0%olive oil and were also successfully infected by T.matsutake isolate A in the soil containing 1.0%olive oil.This study estab-lished a culture system of artificial Shiro formation and also provided a premise for formulation of culture substratum for fruit body formation of T.matsutake.