Differential Expression of Genes Related to Fruit Development and Capsaicinoids Synthesis in Habanero Pepper Plants Grown in Contrasting Soil Types

Habanero pepper(Capsicum chinense Jacq.)is a crop of economic relevance in the Peninsula of Yucatan.Its fruits have a high level of capsaicinoids compared to peppers grown in other regions of the world,which gives them industrial importance.Soil is an important factor that affects pepper development,nutritional quality,and capsaicinoid content.However,the effect of soil type on fruit development and capsaicinoid metabolism has been little understood.This work aimed to compare the effect of soils with contrasting characteristics,black soil(BS)and red soil(RS),on the expression of genes related to the development of fruits,and capsaicinoid synthesis using a transcriptomic analysis of the habanero pepper fruits.Plants growing in RS had bigger fruits and higher expression of genes related to floral development,fruit abscission,and softening which suggests that RS stimulates fruit development from early stages until maturation stages.Fruits from plants growing in BS had enrichment in metabolic pathways related to growth,sugars,and photosynthesis.Besides,these fruits had higher capsaicinoid accumulation at 25 days post-anthesis,and higher expression of genes related to the branched-chain amino acids metabolism(ketol-acid reductisomerase KARI),pentose phosphate pathway and production of NADPH(glucose-6-phosphate-1-dehydrogenase G6PDH),and proteasome and vesicular traffic in cells(26S proteasome regulatory subunit T4 RPT4),which suggest that BS is better in the early stimulation of pathways related to the nutritional quality and capsaicinoid metabolism in the fruits.

Soil Organic Carbon Stock Variation under Different Soil Types and Land Uses in the Sub-Humid Noun Plain, Western Cameroon

This study was conducted to assess the current stock of soil organic carbon under different agricultural land uses, soil types and soil depths in the Noun plain in western Cameroon. Three sites were selected for the study, namely Mangoum, Makeka and Fossang, representative of the three dominant soil types of the noun plain (Andosols, Acrisols and Ferralsols). Three land uses were selected per site including natural vegetation, agroforest and crop field. Soil was sampled at three depths;0 - 20 cm, 20 - 40 cm, and 40 - 60 cm. Analysis of variance showed that soil type did not significantly influence carbon storage, but rather land uses and soil depth. SOCS decreased significantly with depth in all the sites, with an average stock of 66.3 ± 15.8 tC/ha at 0 - 20 cm, compared to an average stock of 33.3 ± 7.4 tC/ha at 40 - 60 cm. SOCS was significantly highest in the natural formation with 57.2 ± 19.7 tC/ha, and lowest in cultivated fields, at 37.7 ± 10.6 tC/ha. Andosols, with their high content of coarse fragments, stored less organic carbon than Ferralsols and Acrisols.

THE INFLUENCE OF FERTILIZERS, NaHCO_3,SOIL TYPE ON CHEMISTRY OF FLOODED SOILS AND RICE PLANT GROWTH

This research was conducted to study the influence of NaHCO3and fertilizer treatments on flooded soils tothe chemistry of soil and rice plant growth.The experiment was done in a greenhouse using a split-split plot design ofthree factors: two types of soil,three doses of fertilizers and three levels of NaHCO3with three replications.The firstfactor was two kind of soils Fluvaquent and Calciquert,the factor was without nutrient(blank),macro nutrients,andcompletely nutrients(second macro and micro nutrients),and the third factor was 0 mg/kg,200 mg/kg and 400 mg/kgof NaHCO3or equivalent with 0 mg,1 200 mg and 2 400 mg NaHCO3per pot.Each experimental unit used a pot of 10L capacity and filled with soil mud equivalent with 6 kg of dried soil.After each of treatment combination mixed into themud soil,two plants of IR-32 variety were grown,and flooded at5 cm high accordingly with growth period.Parametersobserved were the growth of tillers,electrical conductivity(E.C.) and pH.The experiment results showed that the treatment of NaHCO3increased the soluble salt content(E.C.) and soilacidity(pH) during flooding on Calciquerts and Fluvaquents.The content of soluble salt decreased to 2.0 mmhos/cm,while pHon Calciquert decreased towards the neutral pH.Anyhowthe pHon Fluvaquents increased approach to neutral.The treatments of NaHCO3inhibited the tiller growth,either macro fertilizer and completely fertilizer increased thetillers,but did not improve the negative effect of alkalinity due to of NaHCOtreatment.

Study on strength properties and soil behaviour type classification of Huanghe River Delta silts based on variable rate piezocone penetration test

Fine-grained silt is widely distributed in the Huanghe River Delta(HRD)in China,and the sedimentary structure is complex,meaning that the clay content in the silt is variable.The piezocone penetration test(CPTu)is the most widely approved in situ test method.It can be used to invert soil properties and interpret soil behavior.To analyse the strength properties of surface sediments in the HRD,this paper evaluated the friction angle and its inversion formula through the CPTu penetration test and monotonic simple shear test and other soil unit experiments.The evaluation showed that the empirical formula proposed by Kulhawy and Mayne had better prediction and inversion effect.The HRD silts with clay contents of 9.2%,21.4%and 30.3%were selected as samples for the CPTu variable rate penetration test.The results show as follows.(1)The effects of the clay content on the tip resistance and the pore pressure of silt under different penetration rates were summarized.The tip resistance Q_t is strongly dependent on the clay content of the silt,the B_(q)value of the silt tends to 0 and is not significantly affected by the change of the CPTu penetration rate.(2)Five soil behavior type classification charts and three soil behavior type indexes based on CPTu data were evaluated.The results show that the soil behavior type classification chart based on soil behavior type index ISBT,the Robertson 2010 behavior type classification chart are more suitable for the silty soil in the HRD.

Oviposition Site Preference and Its Effects on Subsequent Development of Variegated Grasshopper (Zonocerus variegatus L.) under Laboratory Conditions

Female grasshoppers can affect the fitness of their offspring through their selection of oviposition site. Knowledge of soil type on oviposition, and its effects on subsequent development can provide guidelines for habitat manipulations that reduce the harmful effects of these pests on farmers fields. The influence of soil types on the oviposition site preference of variegated grasshopper (Zonocerus variegatus L.) reared some cassava (Manihot esculenta Crantz) varieties, was investigated in a cage trial carried out at the Bio factory laboratory, School of Agriculture and Food Sciences, Njala University, Sierra Leone during 2022/2023. The treatments comprised three soil types (Sandy, Loamy and Clay), each with three replications laid out in a randomized complete block design (RCBD) in wooden cages. Data were collected on the following development parameters including, Net reproductive growth ratio (R0), Generation time (Tc), Intrinsic rate of increase (rm), Finite rate of increase (), Doubling time (Dt), and overall survivorship. Findings revealed that, Z. variegatus L. preferred sandy soil in which, on average, most eggs were deposited (338, 6.62 4.40), followed by loamy soil, 286 (5.53 3.96), and then, clayey soil, 200 (3.91 3.85);though, the differences were not significant. This study established that Z. variegatus deposited more eggs in sandy soil > loamy soil > clayey soil, respectively;and subsequent survivorship of the immature unto mature adult insect, revealed a similar order. This indicates that the sandy soil is the most preferred substrate for oviposition and subsequent development into adult insects.

Influence of water potential and soil type on conventional japonica super rice yield and soil enzyme activities

We carried out a pool culture experiment to determine the optimal water treatment depth in loam and clay soils during the late growth stage of super rice. Three controlled water depth treatments of 0-5, 0-10 and 0-15 cm below the soil surface were established using alternate wetting and drying irrigation, and the soil water potential （0 to -25 kPa） was measured at 5, 10 and 15 cm. A 2-cm water layer was used as the control. We measured soil enzyme activities, root antioxidant enzyme activities, chlorophyll fluorescence parameters, and rice yield. The results showed that the 0-5-cm water depth treatment significantly increased root antioxidant enzyme activities in loam soil compared with the control, whereas soil enzyme activities, chlorophyll fluorescence parameters and yield did not differ from those of the control. The 0-10- and 0-15-cm water depth treatments also increased root antioxidant enzyme activities, whereas soil enzyme activities, chlorophyll fluorescence parameters and yield decreased. In clay soil, the soil enzyme activities, root antioxidant enzyme activities, chlorophyll fluorescence parameters, and yield did not change with the 0-5-cm water treatment, whereas the 0-10- and 0-15-cm water treatments improved these parameters. Therefore,the appropriate depths for soil water during the late growth period of rice with a 0 to -25 kPa water potential were 5 cm in loam and 15 cm in clay soil.

Change of soil productivity in three different soils after long-term field fertilization treatments

Soil productivity(SP) without external fertilization influence is an important indicator for the capacity of a soil to support crop yield.However,there have been difficulties in estimating values of SPs for soils after various long-term field treatments because the treatment without external fertilization is used but is depleted in soil nutrients,leading to erroneous estimation.The objectives of this study were to estimate the change of SP across different cropping seasons using pot experiments,and to evaluate the steady SP value(which is defined by the basal contribution of soil itself to crop yield) after various longterm fertilization treatments in soils at different geographical locations.The pot experiments were conducted in Jinxian of Jiangxi Province with paddy soil,Zhengzhou of Henan Province with fluvo-aquic soil,and Gongzhuling of Jilin Province with black soils,China.Soils were collected after long-term field fertilization treatments of no fertilizer(control;CK-F),chemical fertilizer(NPK-F),and combined chemical fertilizer with manure(NPKM-F).The soils received either no fertilizer(F0) or chemical fertilizer(F1) for 3-6 cropping seasons in pots,which include CK-P(control;no fertilizer from long-term field experiments for pot experiments),NPK-P(chemical fertilizer from long-term field experiments for pot experiments),and NPKM-P(combined chemical and organic fertilizers from long-term field experiments for pot experiments).The yield data were used to calculate SP values.The initial SP values were high,but decreased rapidly until a relatively steady SP was achieved at or after about three cropping seasons for paddy and fluvo-aquic soils.The steady SP values in the third cropping season from CK-P,NPK-P,and NPKM-P treatments were 37.7,44.1,and 50.0% in the paddy soil,34.2,38.1,and 50.0% in the fluvo-aquic soil,with the highest value observed in the NPKM-P treatment for all soils.However,further research is required in the black soils to incorporate more than three cropping seasons.The partial least squares path mode(PLS-PM) showed that total N(nitrogen) and C/N ratio(the ratio of soil organic carbon and total N) had positive effects on the steady SP for all three soils.These findings confirm the significance of the incorporation of manure for attaining high soil productivity.Regulation of the soil C/N ratio was the other main factor for steady SP through fertilization management.

Spatial patterns of insect herbivory within a forest landscape:the role of soil type and forest stratum

Background:Insect herbivory has profound impacts on ecosystem processes and services.Although many efforts have been made to recognize the main drivers of insect herbivory at different scales,the results are inconsistent.One likely reason is that studies have insufficiently captured the spatially heterogeneous factors such as soil type and forest stratum within the stand that may significantly affect insect herbivory.In particular,there is a lack of studies that address the detailed spatial patterns of insect herbivory which are influenced by these factors.Methods:We measured the detailed spatial patterns of insect herbivory on cork oak(Quercus variabilis Bl.)in response to soil type(gravel soil and loam)and forest stratum(the upper,lower,and sapling stratum),and correlated these patterns with a set of influencing factors(litter coverage,coverage of shrubs and herbs,soil nutrients,soil moisture,and leaf traits)in a forest landscape.Results:Generally,insect herbivory was spatially heterogeneous within stands.Herbivory was significantly lower in gravel soil areas than in loam soil areas and the highest herbivory occurred in the lower stratum.However,there were also 41 individual plots in which the highest herbivory occurred in the upper stratum and 29 plots in which the highest herbivory occurred in the sapling stratum.There were significant differences in soil nutrient and water status between soil types,but no significant differences in leaf traits.The effects of forest stratum on leaf traits were also inconsistent with those on insect herbivory.Conclusions:Leaf traits may not be the main factors influencing insect herbivory in the field.Soil type may have major effects on herbivory patterns by influencing litter coverage while higher coverage of shrubs and herbs may reduce herbivory in the sapling stratum.These findings may advance our understanding of tree-herbivore interactions in real-world situations and have important implications for the sustainable management of forest ecosystems.

Impact of Soil Type Used in Tunneling on Land Subsidence and Mobility Effective Time under Different Earthquake Records

During recent decades, tunnels construction in urban environments has been recognized as a useful factor in improving transportation situation in cities. Construction of underground structures like tunnels has a direct impact on the above-ground structures. The most important impacts are soil crust movement of the structure movement), acceleration change of the earthquake on structures, and land subsidence. The present research aims at seismic evaluation of the effect of soil type in land subsidence and effective mobility time in tunneling projects. For this purpose, two different soil types are chosen to model and assess factors using numerical methods. The outcome of this research concludes that tunneling changes in the dominant frequency record if these changes reach to the extent that structure frequency equals to record frequency, resonance phenomenon happens.

REGIONAL DIFFERENTIATION OF CRITICAL CONCENTRATION OF ARSENIC(As) AND CADMIUM (Cd) IN MAIN SOIL TYPES OF CHINA

Studies of critical concentration distribution features of polluted elements of Cd and As in main soil types of China, such as laterite, crimson soil, purplish soil, yellow brown soil, brunisolic soil, black soil, cinnamon soil and sierozem revealed that soil types vary in different zones, so do their critical concentrations.The critical concentration has a zonal differentiation with the distribution of soil types from south to north, and from east to west. The critical concentration of Cd increases from south to north (yellow brown soil,brunisolic soil and cinnamon soil),and from east to west (black soil and sierozem). This coincides With changes of climate zones and soil zones. On the contrary, the critical concentration of Asdecreases from south to north and from east to west.

RATIONAL UTILIZATION OF MOUNTAIN SOILS IN SOUTHEAST QINGHAI-XIZANG (TIBETAN) PLATEAU

The southeast Qinghaicozang (Tibetan) Plateau is a physcal mpon ofvery complicated eco-envirorunent with optimum hydrothermal conditions. Thisregon conains not ouly vast expanse of alpine soils but also abounds in mosttypes of Chinese forest soils. The distributon and tallization of soils presents avery evident horizontal-vertical zonality. At present, panial soil resources arebeing damaged and the mountain ecology also tends to be instable. So rationalcuttin and forest conservation, barren mountain afferestation, retuming thecultivated land on stop slopes to forest, controlled graking, and soil ameliorationconstitute importan means for rational use of soil resources and improvement andstabilization of mountain ecology in tyis region

Soil erosion types in the loess hill and gully area of China

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Methodology for the Calculation of the Runoff Coefficient with the Arrangement Tirado

For this research work, an adequate methodology was sought for the calculation of the runoff coefficient with the Tirado arrangement. To achieve this, first, the variables that affect the runoff coefficient were identified, then the model was described with the Tirado arrangement, and as a third part for the calculation of the runoff coefficient, the Tirado model is proposed. From the theory for the calculation of the runoff coefficient, the equation of the weighted coefficients and the expression of Nadal were manipulated, resulting in the following relationship , considering this as the expression for the arrangement Tirado. The expression is tested with different intensities, the magnitudes correspond to 150, 200, 250 and 300 mm/hrs, resulting in runoff coefficient 0.82, 0.87, 0.89, 0.91 respectively. This means that, the higher the intensity, the runoff coefficient will be higher, logically the characteristics of the basin affect that this coefficient has variation in the space studied.

Methodology for Obtaining Optimal Sleeve Friction and Friction Ratio Estimates from CPT Data

Cone penetration testing (CPT) is a cost effective and popular tool for geotechnical site characterization. CPT consists of pushing at a constant rate an electronic penetrometer into penetrable soils and recording cone bearing (qc), sleeve friction (fc) and dynamic pore pressure (u) with depth. The measured qc, fs and u values are utilized to estimate soil type and associated soil properties. A popular method to estimate soil type from CPT measurements is the Soil Behavior Type (SBT) chart. The SBT plots cone resistance vs friction ratio, Rf [where: Rf = (fs/qc)100%]. There are distortions in the CPT measurements which can result in erroneous SBT plots. Cone bearing measurements at a specific depth are blurred or averaged due to qc values being strongly influenced by soils within 10 to 30 cone diameters from the cone tip. The qcHMM algorithm was developed to address the qc blurring/averaging limitation. This paper describes the distortions which occur when obtaining sleeve friction measurements which can in association with qc blurring result in significant errors in the calculated Rf values. This paper outlines a novel and highly effective algorithm for obtaining accurate sleeve friction and friction ratio estimates. The fc optimal filter estimation technique is referred to as the OSFE-IFM algorithm. The mathematical details of the OSFE-IFM algorithm are outlined in this paper along with the results from a challenging test bed simulation. The test bed simulation demonstrates that the OSFE-IFM algorithm derives accurate estimates of sleeve friction from measured values. Optimal estimates of cone bearing and sleeve friction result in accurate Rf values and subsequent accurate estimates of soil behavior type.

Distribution of antibiotic resistant bacteria in different soil types following manure application

ARB was investigated in different soil types following manure application.CTC-manure induced more resistance of soil indigenous microbes in fluvo-aquic soil.Lactobacillus,Dyella,Ralstonia,and Bacillus were the key different genera.Manure control is an effective way to reduce the risk of soil ARB.

Factors Influencing the Acidification Trends in Agriculture Soils: A Case Study of Slovakia

This study aims to evaluate the development of soil reaction values in 15 key localities of soil Partial Monitoring System from 1994 to 2023, and to identify the most important regional drivers of pH value development. Soil samples were collected from the depth 0 - 0.10 m yearly in the spring (5 samples from each locality). In the dry soil, samples were determined actively and exchanged soil reaction. The most significant negative changes (decreases of soil reaction) were determined in Haplic Stagnosols group and Cambisols group. The pH value in topsoil is primarily controlled by soil type and soil substrate, soil management and land use, and to a lesser extent by climatic region.

Soil carbon pool in China and its global significance

Soil organic carbon density and its related characteristics of 41 soil types all over China were analyzed by using data of 745 soil profiles , and size of soil carbon pool was estimated. As a result, area-weighted averages of these 41 soil types for bulk density, profile depth, organic carbon content and profile carbon were 1. 24 tC/m3, 86. 2 cm, 3. 04% and 19. 7 kg C/m2 respectively. Total size of soil carbon pool was 185. 68 × 1009tC, which is 29 times of that in terrestrial biomass of China and 12. 6% of global soil carbon pools. Because of its huge carbon pool, soil of China plays an important role in global carbon cycle.

The Estimation of Soil Trace Elements Distribution and Soil-Plant-Animal Continuum in Relation to Trace Elements Status of Sheep in Huangcheng Area of Qilian Mountain Grassland,China

The purpose of the present study was to survey contents of trace elements of Cu, Mn, Fe, and Zn in the surface layer （0-20 cm） in the soil, pasture and serum of sheep in Huangcheng area of Qilian mountain grassland, China. Also the soil-plantanimal continuum was analyzed. Soil （n=300）, pasture （n=60）, and blood serum samples from sheep （n=480） were collected from Huangcheng area of Qilian mountain grassland, China. The contents of trace element in the samples were analyzed by atomic absorption spectrophotometer after digestion. The soil trace elements density distribution shows a ladder-like pattern distribution. Equations developed in the present study for prediction of Fe （R2=0.943） and Zn （R2=0.882） had significant R2 values.

Effects of Nitrogen Application Levels on Ammonia Volatilization and Nitrogen Utilization during Rice Growing Season

We conducted field trials of rice grown in sandy soil and clay soil to determine the effects of nitrogen application levels on the concentration of NH4＋-N in surface water, loss of ammonia through volatilization from paddy fields, rice production, nitrogen-use efficiency, and nitrogen content in the soil profile. The concentration of NH4＋-N in surface water and the amount of ammonia lost through volatilization increased with increasing nitrogen application level, and peaked at 1-3 d after nitrogen application. Less ammonia was lost via volatilization from clay soil than from sandy soil. The amounts of ammonia lost via volatilization after nitrogen application differed depending on the stage when it was applied, from the highest loss to the lowest： N application to promote tillering 〉 the first N topdressing to promote panicle initiation （applied at the last 4-leaf stage） 〉 basal fertilizer 〉 the second N topdressing to promote panicle initiation （applied at the last 2-leaf stage）. The total loss of ammonia via volatilization from clay soil was 10.49-87.06 kg/hm2, equivalent to 10.92%-21.76% of the nitrogen applied. The total loss of ammonia via volatilization from sandy soil was 11.32-102.43 kg/hm2, equivalent to 11.32%-25.61 % of the nitrogen applied. The amount of ammonia lost via volatilization and the concentration of NH4＋-N in surface water peaked simultaneously after nitrogen application; both showed maxima at the tillering stage with the ratio between them ranging from 23.76% to 33.65%. With the increase in nitrogen application level, rice production and nitrogen accumulation in plants increased, but nitrogen-use efficiency decreased. Rice production and nitrogen accumulation in plants were slightly higher in clay soil than in sandy soil. In the soil, the nitrogen content was the lowest at a depth of 40-50 cm. In any specific soil layer, the soil nitrogen content increased with increasing nitrogen application level, and the soil nitrogen content was higher in clay soil than in sandy soil. In terms of ammonia volatilization, the amount of ammonia lost via volatilization increased markedly when the nitrogen application level exceeded 250 kg/hm2 in the rice growing season. However, for rice production, a suitable nitrogen application level is approximately 300 kg/hm2. Therefore, taking the needs for high crop yields and environmental protection into account, the appropriate nitrogen application level was 250-300 kg/hm2 in these conditions.

A site dependent top height growth model for hybrid aspen

In this study height growth models for hybrid aspen were developed using three growth equations. The mean age of the hybrid aspen was 21 years （range 15-51 years） with a mean stand density of 946 stems ha-~ （87-2374） and a mean diameter at breast height （over bark） of 19.6 cm （8.5-40.8 cm）. Site index was also examined in relation to soil type. Multiple samples were collected for three types of soil： light clay, medium clay and till. Site index curves were constructed using the col- lected data and compared with published reports. A number of dynamic equations were assessed for modeling top-height growth from total age.