[Objective] To research the mass propagation system for cotyledon of Solanum torvum. [Methods] With cotyledon of S. torvum as the research object, ef- fects of hormone combination on callus induction and adventitious ...[Objective] To research the mass propagation system for cotyledon of Solanum torvum. [Methods] With cotyledon of S. torvum as the research object, ef- fects of hormone combination on callus induction and adventitious buds differentia- tion of S. torvum were researched. [Results] With cotyledon of S. torvum as the ex- plants, the optimal culture medium for callus induction and adventitious buds differ- entiation was MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA. The induction rates of callus and adventitious bud reached 100% and 85%, respectively. The number of average buds was 6. The optimal culture medium for the induction of adventitious roots was MS＋ 0.3 mg/L IAA. The rooting rate reached 100% and the number of average roots was 9. [Conclusions] One-step induction of callus and adventitious buds simplified the mass propagation system, and enhanced the test test efficiency.s展开更多
Verticillium wilt is a severe disease in eggplant caused by Verticillium dahliae.Polygalacturonase-inhibiting proteins （PGIPs） have been shown to be involved in preventing the invasion of fungus including V.dahliae....Verticillium wilt is a severe disease in eggplant caused by Verticillium dahliae.Polygalacturonase-inhibiting proteins （PGIPs） have been shown to be involved in preventing the invasion of fungus including V.dahliae.Cloning genes encoding PGIPs is quite valuable for plant resistance breeding to Verticillium wilt.In this study,a cDNA encoding the polygalacturonase-inhibiting protein was isolated from Solanum torvum by RT-PCR and RACE,designated StPGIP （accession no.FJ943498）.The cDNA sequence of StPGIP was 1 097 bp long and contained an open reading frame of 990 bp.The predicted amino acid sequence of the gene consisted of 329 amino acids and had conserved LRRs.The StPGIP protein had a high identity with PGIPs from other species.Analysis of StPGIP expression at the mRNA level by RT-PCR showed that the gene was expressed in all organs and could be induced to increase expression by V.erticillium dahliae infection.展开更多
Objective:To study the effect of water extract of Solanum torvum(S.torvum) on blood lipid and sex hormone levels in high-fat diet(HFD) fed male rats.Methods:Male Wistar rats were maintained on a standard diet or HFD f...Objective:To study the effect of water extract of Solanum torvum(S.torvum) on blood lipid and sex hormone levels in high-fat diet(HFD) fed male rats.Methods:Male Wistar rats were maintained on a standard diet or HFD for 10 weeks.During the last 4 weeks,the standard diet groups received distilled water or S.torvum(400 mg/kg) and the HFD groups received distilled water or S.torvum(100,200 and400 mg/kg).Body weight,lipid profiles,sex hormone,internal organs weight and liver histopathology were all measured.Moreover,kidney function was evaluated using blood urea nitrogen and creatinine levels,and liver function by the levels of aspartate aminotransferase and alanine aminotransferase.Results:The result showed that rats in the HFD control group had increased body weight and hyperlipidemia,but had decreased levels of both testosterone and estradiol.When receiving the S.torvum extract at a dose of 100 mg/kg,treated rats had significantly increased sex hormone levels of both types,and decreased total cholesterol levels,and at a dose of 200 mg/kg,treated rats had significantly decreased levels of triglyceride.Long term administration of the S.torvum did not produce any toxic signs in livers and kidneys.Pathological examinations of livers showed lipid accumulation in the HFD group,but the treatment of S.torvum slightly reduced lipid deposition in liver tissue.Conclusions:S.torvum extract can reverse the level of sex hormones to their normal level and reduce serum cholesterol in HFD-induced obese male rats.Furthermore,the long term oral administration of S.torvum extract is harmless.展开更多
Objective: To evaluate the phytochemical constituents, antimicrobial and antioxidant activities of the extracts of Distemonanthus benthamianus(D. benthamianus) stem bark and Solanum torvum(S. torvum) fruit which have ...Objective: To evaluate the phytochemical constituents, antimicrobial and antioxidant activities of the extracts of Distemonanthus benthamianus(D. benthamianus) stem bark and Solanum torvum(S. torvum) fruit which have been used as traditional medicinal herbs in Gabon. Methods: Plant extracts were subjected to a qualitative study(phytochemical screening) and a quantitative(dosing) study of secondary metabolites. Antioxidant activity was tested by 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid assay. Bacteria and fungi susceptibility tests were performed on Mueller Hinton medium and solid Sabouraud, respectively, using the diffusion method, while minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration were evaluated by microdilution method.Results: The total phenol and tannin contents were significantly higher in the water-ethanol extract compared to the other extracts of D. benthamianus and S. torvum. The water-ethanol and water-acetone extracts showed significantly higher antioxidant activity than the aqueous extracts of the two medicinal plants. However, the extracts presented weak antioxidant activities compared to standards(Vitamin C, BHA). The water-acetone and water-ethanol extracts of S. torvum showed the highest antimicrobial activity against Bacillus cereus LMG 13569 BHI, Shigella dysenteriae 5451 CIP, Shigella dysenteriae and Neisseria gonorrhoeae. Conclusions: Our results show that D. benthamianus and S. torvum can be promising sources of natural products with potential antimicrobial and antioxidant activities.展开更多
基金Supported by the"Twelfth Five Year Plan"National Science and Technology Plan Project of Rural Areas in China(2012AA100103007)the Transformation Projects of National Agricultural Science and Technology Achievements(2013GB2E100381)+2 种基金the Guangxi Innovation Team Project of Staple Vegetable of Modern Agricultural Industry Technology System(nycytxgxcxtd-03-10)the Science and Technology Planning Project of Guangxi(14123006-35,14123004-3-5)the Special Fund for Basal Research in Guangxi Academy of Agricultural Sciences(2012YT05,2015YT67)~~
文摘[Objective] To research the mass propagation system for cotyledon of Solanum torvum. [Methods] With cotyledon of S. torvum as the research object, ef- fects of hormone combination on callus induction and adventitious buds differentia- tion of S. torvum were researched. [Results] With cotyledon of S. torvum as the ex- plants, the optimal culture medium for callus induction and adventitious buds differ- entiation was MS＋2.0 mg/L 6-BA＋0.3 mg/L NAA. The induction rates of callus and adventitious bud reached 100% and 85%, respectively. The number of average buds was 6. The optimal culture medium for the induction of adventitious roots was MS＋ 0.3 mg/L IAA. The rooting rate reached 100% and the number of average roots was 9. [Conclusions] One-step induction of callus and adventitious buds simplified the mass propagation system, and enhanced the test test efficiency.s
基金financially supported by grants from the State Key Laboratory of Crop Genetics and Germplasm Enhancement,the Ministry of Sciences and Technology of the People's Republic of China (ZW200703)the Key Laboratory of Cell Engineering, Institute of Nuclear and Biotechnology, Xinjiang Academy of Agricultural Sciences, China (XJYS0302-200601)
文摘Verticillium wilt is a severe disease in eggplant caused by Verticillium dahliae.Polygalacturonase-inhibiting proteins （PGIPs） have been shown to be involved in preventing the invasion of fungus including V.dahliae.Cloning genes encoding PGIPs is quite valuable for plant resistance breeding to Verticillium wilt.In this study,a cDNA encoding the polygalacturonase-inhibiting protein was isolated from Solanum torvum by RT-PCR and RACE,designated StPGIP （accession no.FJ943498）.The cDNA sequence of StPGIP was 1 097 bp long and contained an open reading frame of 990 bp.The predicted amino acid sequence of the gene consisted of 329 amino acids and had conserved LRRs.The StPGIP protein had a high identity with PGIPs from other species.Analysis of StPGIP expression at the mRNA level by RT-PCR showed that the gene was expressed in all organs and could be induced to increase expression by V.erticillium dahliae infection.
基金Supported by Thammasat University(Contract number:GEN2/50/2013)
文摘Objective:To study the effect of water extract of Solanum torvum(S.torvum) on blood lipid and sex hormone levels in high-fat diet(HFD) fed male rats.Methods:Male Wistar rats were maintained on a standard diet or HFD for 10 weeks.During the last 4 weeks,the standard diet groups received distilled water or S.torvum(400 mg/kg) and the HFD groups received distilled water or S.torvum(100,200 and400 mg/kg).Body weight,lipid profiles,sex hormone,internal organs weight and liver histopathology were all measured.Moreover,kidney function was evaluated using blood urea nitrogen and creatinine levels,and liver function by the levels of aspartate aminotransferase and alanine aminotransferase.Results:The result showed that rats in the HFD control group had increased body weight and hyperlipidemia,but had decreased levels of both testosterone and estradiol.When receiving the S.torvum extract at a dose of 100 mg/kg,treated rats had significantly increased sex hormone levels of both types,and decreased total cholesterol levels,and at a dose of 200 mg/kg,treated rats had significantly decreased levels of triglyceride.Long term administration of the S.torvum did not produce any toxic signs in livers and kidneys.Pathological examinations of livers showed lipid accumulation in the HFD group,but the treatment of S.torvum slightly reduced lipid deposition in liver tissue.Conclusions:S.torvum extract can reverse the level of sex hormones to their normal level and reduce serum cholesterol in HFD-induced obese male rats.Furthermore,the long term oral administration of S.torvum extract is harmless.
基金supported by USTM Biochemistry Research Laboratory(SG/CIS/SDM/SA/sa grant n°77)
文摘Objective: To evaluate the phytochemical constituents, antimicrobial and antioxidant activities of the extracts of Distemonanthus benthamianus(D. benthamianus) stem bark and Solanum torvum(S. torvum) fruit which have been used as traditional medicinal herbs in Gabon. Methods: Plant extracts were subjected to a qualitative study(phytochemical screening) and a quantitative(dosing) study of secondary metabolites. Antioxidant activity was tested by 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis 3-ethylbenzothiazoline-6-sulfonic acid assay. Bacteria and fungi susceptibility tests were performed on Mueller Hinton medium and solid Sabouraud, respectively, using the diffusion method, while minimum inhibitory concentration, minimum fungicidal concentration and minimum bactericidal concentration were evaluated by microdilution method.Results: The total phenol and tannin contents were significantly higher in the water-ethanol extract compared to the other extracts of D. benthamianus and S. torvum. The water-ethanol and water-acetone extracts showed significantly higher antioxidant activity than the aqueous extracts of the two medicinal plants. However, the extracts presented weak antioxidant activities compared to standards(Vitamin C, BHA). The water-acetone and water-ethanol extracts of S. torvum showed the highest antimicrobial activity against Bacillus cereus LMG 13569 BHI, Shigella dysenteriae 5451 CIP, Shigella dysenteriae and Neisseria gonorrhoeae. Conclusions: Our results show that D. benthamianus and S. torvum can be promising sources of natural products with potential antimicrobial and antioxidant activities.
