A rapid method for the simultaneous determination of daidzein,genistein and formonetin in solanum Lyratum Thunb by high performance liquid chromatography(HPLC)was developed.Separation was achieved on a Diamonsil C18 c...A rapid method for the simultaneous determination of daidzein,genistein and formonetin in solanum Lyratum Thunb by high performance liquid chromatography(HPLC)was developed.Separation was achieved on a Diamonsil C18 column(250 mm×4.6 mm,5 μm)with isocratic elution,using a mobile phase of methanol-tetrahydrofuran-water(44∶3∶53,v/v).The wavelength was set at 260 nm and column was maintained at 35 ℃.The linear ranges of daidzein,genistein and formonetin were 1.0-40.0,0.1-4.0 and 0.1-4.0 μg/mL,respectively.The average recoveries were between 98.4% and 101.3%.This method could be used for the quality control of Solanum lyratum Thunb due to its simplification,reliability,rapidity and excellent precision.展开更多
Solanum lyratum Thunb.belonging to the family Solanaceae,is a common Chinese herbal medicine in folk.It has been used for the treatment of colds,fever,jaundice hepatitis,cholecystitis,cholelithiasis,nephritis,edema an...Solanum lyratum Thunb.belonging to the family Solanaceae,is a common Chinese herbal medicine in folk.It has been used for the treatment of colds,fever,jaundice hepatitis,cholecystitis,cholelithiasis,nephritis,edema and cancer.Previous phytochemical studies on S.lyratum have led to the identification of many chemical constituents,including steroidal saponins,alkaloids,terpenoids,flavonoids,quinonoids,phenylpropanoids,organic acids and several other kinds of compounds,which showed diverse bioactivities,such as anticancer,anti-inflammatory and antioxidant activities.This paper summarized the primary chemical constituents and pharmacological effects of S.lyratum to provide reference for its further development and utilization.展开更多
Solanum lyratum is one of the temperate plants,broadly distributed in Korea,China,Japan,India,and South-East Asia and well-documented in those oriental ethnic medicine systems for curing cancers,jaundice,edema,gonorrh...Solanum lyratum is one of the temperate plants,broadly distributed in Korea,China,Japan,India,and South-East Asia and well-documented in those oriental ethnic medicine systems for curing cancers,jaundice,edema,gonorrhea,cholecystitis,phlogosis,rheumatoid arthritis,etc.This review systematically summarized the research progress on S.lyratum respecting the botany,traditional uses,phytochemistry,pharmacology,and toxicology to increase people’s in-depth understanding of this plant,by data retrieval in a series of online or off-line electronic databases as far as we can reach.Steroidal saponins and alkaloids,terpenoids,nitrogenous compounds,and flavonoid compounds are the main chemical constituents in S.lyratum.Among them,steroidal alkaloids and saponins are the major active ingredi-ents ever found in S.lyratum,exerting activities of anti-cancer,anti-inflammation,anti-microbial,anti-allergy,and anti-oxidation in vivo or in vitro.As a result,S.lyratum has been frequently prescribed for the abovementioned therapeutic purposes,and there are substantial traditional and modern shreds of evidence of its use.展开更多
目的分离、纯化和鉴定白毛藤S olanum ly ra tum中的多糖的成分。方法经脱脂、沸水抽提、乙醇沉淀、酶-Sevag法脱蛋白得到的粗多糖,再经DEAE-Sepharose fast flow阴离子交换和Sephadex G-200葡聚糖凝胶柱色谱分离得到纯化的白毛藤多糖(S...目的分离、纯化和鉴定白毛藤S olanum ly ra tum中的多糖的成分。方法经脱脂、沸水抽提、乙醇沉淀、酶-Sevag法脱蛋白得到的粗多糖,再经DEAE-Sepharose fast flow阴离子交换和Sephadex G-200葡聚糖凝胶柱色谱分离得到纯化的白毛藤多糖(SLPS),用红外光谱和紫外光谱分析鉴定该多糖,凝胶渗透色谱(GPC)法测定其相对分子质量,运用薄板色谱(TLC)和纸色谱(PC)法初步测其结构组成。结果红外光谱分析具有典型的多糖特征吸收峰,紫外光谱分析未见蛋白质(280 nm)与核酸(260 nm)的特征吸收峰。结论本实验所提取的白毛藤多糖为单一组分的多糖。展开更多
基金supported by Liaoning Technology Gallery(2007226011)Shenyang Technology Division(1071164-9-00)
文摘A rapid method for the simultaneous determination of daidzein,genistein and formonetin in solanum Lyratum Thunb by high performance liquid chromatography(HPLC)was developed.Separation was achieved on a Diamonsil C18 column(250 mm×4.6 mm,5 μm)with isocratic elution,using a mobile phase of methanol-tetrahydrofuran-water(44∶3∶53,v/v).The wavelength was set at 260 nm and column was maintained at 35 ℃.The linear ranges of daidzein,genistein and formonetin were 1.0-40.0,0.1-4.0 and 0.1-4.0 μg/mL,respectively.The average recoveries were between 98.4% and 101.3%.This method could be used for the quality control of Solanum lyratum Thunb due to its simplification,reliability,rapidity and excellent precision.
文摘Solanum lyratum Thunb.belonging to the family Solanaceae,is a common Chinese herbal medicine in folk.It has been used for the treatment of colds,fever,jaundice hepatitis,cholecystitis,cholelithiasis,nephritis,edema and cancer.Previous phytochemical studies on S.lyratum have led to the identification of many chemical constituents,including steroidal saponins,alkaloids,terpenoids,flavonoids,quinonoids,phenylpropanoids,organic acids and several other kinds of compounds,which showed diverse bioactivities,such as anticancer,anti-inflammatory and antioxidant activities.This paper summarized the primary chemical constituents and pharmacological effects of S.lyratum to provide reference for its further development and utilization.
基金funded by a grant (No.21ZYJDJC00080) from the Tianjin Committee of Science and Technology of Chinathe National Key Research and Development Project of China (No.2018YFC1707904,2018YFC1707905,and 2018YFC1707403)the Important Drug Development Fund,Ministry of Science and Technology of China (No.2018ZX09735-002).
文摘Solanum lyratum is one of the temperate plants,broadly distributed in Korea,China,Japan,India,and South-East Asia and well-documented in those oriental ethnic medicine systems for curing cancers,jaundice,edema,gonorrhea,cholecystitis,phlogosis,rheumatoid arthritis,etc.This review systematically summarized the research progress on S.lyratum respecting the botany,traditional uses,phytochemistry,pharmacology,and toxicology to increase people’s in-depth understanding of this plant,by data retrieval in a series of online or off-line electronic databases as far as we can reach.Steroidal saponins and alkaloids,terpenoids,nitrogenous compounds,and flavonoid compounds are the main chemical constituents in S.lyratum.Among them,steroidal alkaloids and saponins are the major active ingredi-ents ever found in S.lyratum,exerting activities of anti-cancer,anti-inflammation,anti-microbial,anti-allergy,and anti-oxidation in vivo or in vitro.As a result,S.lyratum has been frequently prescribed for the abovementioned therapeutic purposes,and there are substantial traditional and modern shreds of evidence of its use.
文摘目的分离、纯化和鉴定白毛藤S olanum ly ra tum中的多糖的成分。方法经脱脂、沸水抽提、乙醇沉淀、酶-Sevag法脱蛋白得到的粗多糖,再经DEAE-Sepharose fast flow阴离子交换和Sephadex G-200葡聚糖凝胶柱色谱分离得到纯化的白毛藤多糖(SLPS),用红外光谱和紫外光谱分析鉴定该多糖,凝胶渗透色谱(GPC)法测定其相对分子质量,运用薄板色谱(TLC)和纸色谱(PC)法初步测其结构组成。结果红外光谱分析具有典型的多糖特征吸收峰,紫外光谱分析未见蛋白质(280 nm)与核酸(260 nm)的特征吸收峰。结论本实验所提取的白毛藤多糖为单一组分的多糖。
