Identification of candidate genes related to soluble sugar contents in soybean seeds using multiple genetic analyses

Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci(QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published papers. After meta-overview-collinearity integrated analysis to refine QTL intervals, eight consensus QTLs were identified. To further verify the consensus QTLs, a population of chromosome segment substitution lines(CSSLs) was analyzed. Two lines containing fragments covering the regions of consensus QTLs and the recurrent parent were selected: one line showed high soluble sugar contents associated with a consensus QTL fragment, and the other line showed low soluble sugar contents. Transcriptome sequencing was conducted for these two lines at the early, middle, and late stages of seed development, which identified 158, 109 and 329 differentially expressed genes, respectively. Based on the analyses of re-sequencing data of the CSSLs and the consensus QTL region, three candidate genes(Glyma.19 G146800, Glyma.19 G122500, and Glyma.19 G128500) were identified in the genetic fragments introduced from wild soybean. Sequence comparisons between the two CSSL parents SN14 and ZYD00006 revealed a single nucleotide polymorphism(SNP) mutation in the coding sequence of Glyma.19 G122500, causing a nonsynonymous mutation in the amino acid sequence that affected the predicted protein structure. A Kompetitive allele-specific PCR(KASP) marker was developed based on this SNP and used to evaluate the CSSLs. These results lay the foundation for further research to identify genes related to soluble sugar contents in soybean seeds and for future soybean breeding.

Effects of Aluminum Toxicity on Physiological Indices of Jatropha curcas L.Seedling

Jatropha curcas was taken as the test material,6 concentrations including 0,25,50,100,200 and 400μmol/L AlCl3,plus 3 time gradients including 7,14 and 21 d,were set to study the effects of Al^(3+)stress on the antioxidant system of Jatropha curcas L.seedling.The results showed that with the Al^(3+)treatment being applied,protein content increased first,then decreased and finally increased with the increase of Al^(3+)concentration;the soluble sugar content increased first and then decreased with the increase of Al^(3+)concentration.Under low concentration of Al^(3+)treatment,Pro content,MDA content and POD activity of Jatropha curcas L.seedling leaves changed a little,while under high concentration of Al^(3+)treatment,Pro and MDA content of Jatropha curcas L.seedling leaves rapidly accumulated,POD activity increased and they showed a trend of increase with the increase of Al^(3+)concentration;From the perspective of Al^(3+)stress time,protein content,soluble sugar content,MDA content and POD activity increased with stress time being prolonged,while Pro content decreased with stress time being prolonged.These results indicated that the leaves of Jatropha curcas L.seedlings had certain self-protection and remediation abilities under Al^(3+)stress.