Here,a new integrated machine learning and Chou’s pseudo amino acid composition method has been proposed for in silico epitope mapping of severe acute respiratorysyndrome-like coronavirus antigens.For this,a training...Here,a new integrated machine learning and Chou’s pseudo amino acid composition method has been proposed for in silico epitope mapping of severe acute respiratorysyndrome-like coronavirus antigens.For this,a training dataset including 266 linear B-cell epitopes,1,267 T-cell epitopes and 1,280 non-epitopes were prepared.The epitope sequences were then converted to numerical vectors using Chou’s pseudo amino acid composition method.The vectors were then introduced to the support vector machine,random forest,artificial neural network,and K-nearest neighbor algorithms for the classification process.The algorithm with the highest performance was selected for the epitope mapping procedure.Based on the obtained results,the random forest algorithm was the most accurate classifier with an accuracy of 0.934 followed by K-nearest neighbor,artificial neural network,and support vector machine respectively.Furthermore,the efficacies of predicted epitopes by the trained random forest algorithm were assessed through their antigenicity potential as well as affinity to human B cell receptor and MHC-I/II alleles using the VaxiJen score and molecular docking,respectively.It was also clear that the predicted epitopes especially the B-cell epitopes had high antigenicity potentials and good affinities to the protein targets.According to the results,the suggested method can be considered for developing specific epitope predictor software as well as an accelerator pipeline for designing serotype independent vaccine against the virus.展开更多
Fungistasis is one of the important approaches to control soil-borne plant pathogens.Some hypotheses about the mechanisms for soil fungistasis had been established,which mainly focused on the soil bacterial community ...Fungistasis is one of the important approaches to control soil-borne plant pathogens.Some hypotheses about the mechanisms for soil fungistasis had been established,which mainly focused on the soil bacterial community composition,structure,diversity as well as function.In this study,the bacterial community composition and diversity of a series of soils treated by autoclaving,which coming from the same original soil sample and showing gradient fungistasis to the target soil-borne pathogen fungi Fusarium grami...展开更多
This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to ...This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to Bers-type spaces Hv^∞（ or little Bers-type spaces Hv,o∞ ）, where v is normal.展开更多
Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited a...Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for diff erent periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time;abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location;the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional sixfacultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion.展开更多
Song China was a period in which China experienced a great increase in its population.Concurrently,the Song dynasty also experienced a rise in the frequency of epidemics and two major wars with the Western Xia and Lia...Song China was a period in which China experienced a great increase in its population.Concurrently,the Song dynasty also experienced a rise in the frequency of epidemics and two major wars with the Western Xia and Liao dynasties during the 1000s and 1040s.The consequences of these changes were exacerbated by the increased geographical mobility of certain social groups such as traders and examinees attending civil service examinations.Thus,casualties of wars,epidemics,or disease,especially of people whose families were far away and could not care for them were left without care and“their corpses often lay bare along the roads.”This new social environment created a need for general relief.The Northern Song government(960-1127 CE),especially during the reign of Emperor Huizong,established an innovative public health system to address this issue.The public health system included poorhouses,public hospitals,and pauper’s cemeteries.The first were more of charity organizations,whereas the latter two promoted public health by providing medical services for the poor and burial for those that nobody cared for.In terms of rationale behind these institutions,on the one hand,they constituted an attempt to get the poor and homeless off the streets while providing them relief or burial.On the other hand,it seems that Huizong’s deep concern with medicine propelled him to design and implement a comprehensive public health system oriented to prevent contagion and outbreak of epidemics.This article depicts the background,the organization,and the functions of the system.The article also discusses the conditions and reasons that gave rise to such a unique undertaking by the Northern Song government.展开更多
We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice.In addition,we evaluated the production of major cytokines(Interleukin-6 and-10)which are related to inflammation...We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice.In addition,we evaluated the production of major cytokines(Interleukin-6 and-10)which are related to inflammation and fatty acid composition of several tissues.16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed utilizing the EzBioCloud data base.Male mice fed on L.lucidus showed a significantly reduced number of lactic acid bacteria and coliform in the feces compared with the control group(p<0.05).16S rDNA sequencing analysis of fecal samples showed that L.lucidus supplementation decreased the community of harmful microflora(Enterobacteriaceae including Escherichia coli and Bacteroides sp.)in feces compared with the control group(p<0.05).There were no significant differences in mRNA expression of cytokine IL-6 and IL-10 between the control and L.lucidus fed groups.The fecal fatty acid composition in the L.lucidus group had percentages of 4:0,6:0,8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control group.Our results showed that L.lucidus supplementation influenced gut environment by decreasing harmful microflora and increased the percentages of several short fatty acids.展开更多
T.S.Eliot was a leader of the Modernist movement in poetry.His early poem The Love Song of J.Alfred Prufrock reveals the disappointment,loss,confusion and lack of faith that prevailed in Western society after the Worl...T.S.Eliot was a leader of the Modernist movement in poetry.His early poem The Love Song of J.Alfred Prufrock reveals the disappointment,loss,confusion and lack of faith that prevailed in Western society after the World WarⅠ.In this paper,the text of Love Song will be assessed from the perspective of intertextuality through the connection of the fantasy world and the disconnection of the real world.展开更多
We consider the weighted composition operators between Hardy spaces on the unit ball, and obtain some sufficient and necessary conditions of bounded or compact weighted composition operators. We also prove that the op...We consider the weighted composition operators between Hardy spaces on the unit ball, and obtain some sufficient and necessary conditions of bounded or compact weighted composition operators. We also prove that the operator from H^1 to H^1 is compact if and only if it is weakly compact. Meanwhile, we get the analogue on the Bergman spaces.展开更多
We characterize the boundedness and compactness of weighted composition operators on weighted Dirichlet spaces in terms of Nevanlinna counting functions and Caxleson measure.
In this paper, necessary and sufficient conditions for a closed range composition operator CФ on the general family of holomorphic function spaces F（p,q,s） and more generally on α-Besov type spaces F（p,αp-2,s） ...In this paper, necessary and sufficient conditions for a closed range composition operator CФ on the general family of holomorphic function spaces F（p,q,s） and more generally on α-Besov type spaces F（p,αp-2,s） are given. We give a Carleson measure characterization on F （p, αp - 2, s） spaces, then we indicate how Carleson measures can be used to characterize boundedness and compactness of CФ on F（p,q,s） and F（p,αp- 2,s） spaces.展开更多
By analyzing of the existing Web services,an ontology based on OWL is presented,which has rich semantic information,and the service description language OWL-S based on OWL is put forward. OWL-S through IOPE can descri...By analyzing of the existing Web services,an ontology based on OWL is presented,which has rich semantic information,and the service description language OWL-S based on OWL is put forward. OWL-S through IOPE can describe services,can also combine the service,but the method of combination service is not automatic. So a method is presented by using Situation Calculus for automatic service composition based on the OWL-S model. Finally through the example analysis,the method of automatic service combination was validated.展开更多
The gastrointestinal tract, as the major digestive and absorbing organ, plays an important role in chicken growth. Numerous studies have been undertaken to investigate the gut bacterial community in broilers, but litt...The gastrointestinal tract, as the major digestive and absorbing organ, plays an important role in chicken growth. Numerous studies have been undertaken to investigate the gut bacterial community in broilers, but little is known about the archaeal community composition. To improve our understanding of the composition of intestinal archaea and the relationship between archaea and chicken growth, a feeding trial was conducted with 10 000 Ross 308 chicks in a commercial production facility under standard management. At 38 d of age, 15 birds with the highest body weight （ H group）, 15 birds with average body weight （ M group）, and 15 birds with the lowest body weight （ L group） were selected among 600 healthy male individuals. Cecal archaeal community was detected based on 16S rRNA gene sequencing. As a result, 3 321 229 archaeal sequences were clustered into 1 281 operational taxonomic units （OTUs） and classified into three phyla （Crenarchaeola, Euryarchaeota and Thaumarchaeota） and six known genera ： Natronomonas, Halolamina, Salinarchaeum, Cenarchaeum, Candidatus, Nitrosoarchaeum and Nitrososphaera. The alpha indices showed that, the richness of archaea in L group was the highest, and followed by that in M group and H group, while the archaea in H group were more diverse, followed by those in M group and L group. However, the archaeal composition in all chicken samples was similar, with the vast majority of the sequences belonging to Cenarchaeum, and only a few belonging to the other five genera.展开更多
Thin films of ZnxCd1-xS have been prepared by electron beam evaporation of a mixture of ZnS & CdS powders. The films are deposited onto sodalime glass slides under similar conditions.The composition of the films i...Thin films of ZnxCd1-xS have been prepared by electron beam evaporation of a mixture of ZnS & CdS powders. The films are deposited onto sodalime glass slides under similar conditions.The composition of the films is varied from CdS to ZnS (x=0 to 1). The films show a regular change in color from toner red to orange yellow as Zn concentration increases to maximum.These films are characterized for their optical, electricaI and structural properties. The bandgap value of ZnxCd1-xS films is found to vary linearIy from 2.20 eV to 3.44 eV with change in the x value from 0 to 1. The resistivity of these films is in the range of 171.0 Ωcm to 5.5× 106Ωcm for x=0~0.6. All the samples show cubic structure after annealing in air at 250℃ for 40 min.The lattice constant ao varies from 0.5884 nm to 0.54109 nm linearly.展开更多
T.S.Eliot 's poem,"The Love Song of J.Alfred Prufrock",different and unusual,is difficult to interpret.T.S.Eliot rejects logic connection,thus his poems lack logic interpretation.He himself justifies him...T.S.Eliot 's poem,"The Love Song of J.Alfred Prufrock",different and unusual,is difficult to interpret.T.S.Eliot rejects logic connection,thus his poems lack logic interpretation.He himself justifies himself by saying:he wrote it to want it to be difficult.The dissociation of sensibility,on the contrary,arouses the emotion of readers immediately.展开更多
文摘Here,a new integrated machine learning and Chou’s pseudo amino acid composition method has been proposed for in silico epitope mapping of severe acute respiratorysyndrome-like coronavirus antigens.For this,a training dataset including 266 linear B-cell epitopes,1,267 T-cell epitopes and 1,280 non-epitopes were prepared.The epitope sequences were then converted to numerical vectors using Chou’s pseudo amino acid composition method.The vectors were then introduced to the support vector machine,random forest,artificial neural network,and K-nearest neighbor algorithms for the classification process.The algorithm with the highest performance was selected for the epitope mapping procedure.Based on the obtained results,the random forest algorithm was the most accurate classifier with an accuracy of 0.934 followed by K-nearest neighbor,artificial neural network,and support vector machine respectively.Furthermore,the efficacies of predicted epitopes by the trained random forest algorithm were assessed through their antigenicity potential as well as affinity to human B cell receptor and MHC-I/II alleles using the VaxiJen score and molecular docking,respectively.It was also clear that the predicted epitopes especially the B-cell epitopes had high antigenicity potentials and good affinities to the protein targets.According to the results,the suggested method can be considered for developing specific epitope predictor software as well as an accelerator pipeline for designing serotype independent vaccine against the virus.
文摘Fungistasis is one of the important approaches to control soil-borne plant pathogens.Some hypotheses about the mechanisms for soil fungistasis had been established,which mainly focused on the soil bacterial community composition,structure,diversity as well as function.In this study,the bacterial community composition and diversity of a series of soils treated by autoclaving,which coming from the same original soil sample and showing gradient fungistasis to the target soil-borne pathogen fungi Fusarium grami...
基金Supported by the National Natural Science Foundation of China (10771064)the Natural Science Foundation of Zhejiang province (Y6090036+1 种基金Y7080197,Y606197)the Foundation of Department of Education of Zhejiang Province (20070482)
文摘This paper deals with the boundedness and compactness of the weighted composition operators from the F（p, q, s） spaces, including Hardy space, Bergman space, Qp space, BMOA space, Besov space and α-Bloch space, to Bers-type spaces Hv^∞（ or little Bers-type spaces Hv,o∞ ）, where v is normal.
基金Supported by the National Basic Research Program of China(973 Program)(No.2014CB643304)the National Natural Science Foundation of China(No.41576080)the Key Research and Development Program of Shandong Province(No.2018GHY115003)
文摘Anaerobic, aerobic, and facultative bacteria are all present in corrosive environments. However, as previous studies to address corrosion in the marine environment have largely focused on anaerobic bacteria, limited attention has been paid to the composition and function of aerobic and facultative bacteria in this process. For analysis in this study, ten samples were collected from rust layers on steel plates that had been immersed in seawater for diff erent periods (i.e., six months and eight years) at Sanya and Xiamen, China. The cultivable aerobic bacterial community structure as well as the number of sulfate-reducing bacteria (SRB) were analyzed in both cases, while the proportion of facultative SRB among the isolated aerobic bacteria in each sample was also evaluated using a novel approach. Bacterial abundance results show that the proportions are related to sea location and immersion time;abundances of culturable aerobic bacteria (CAB) and SRB from Sanya were greater in most corrosion samples than those from Xiamen, and abundances of both bacterial groups were greater in samples immersed for six months than for eight years. A total of 213 isolates were obtained from all samples in terms of CAB community composition, and a phylogenetic analysis revealed that the taxa comprised four phyla and 31 genera. Bacterial species composition is related to marine location;the results show that Firmicutes and Proteobacteria were the dominant phyla, accounting for 98.13% of the total, while Bacillus and Vibrio were the dominant genera, accounting for 53.06% of the total. An additional sixfacultative SRB strains were also screened from the isolates obtained and were found to encompass the genus Vibrio (four strains), Staphylococcus (one strain), and Photobacterium (one strain). It is noteworthy that mentions of Photobacterium species have so far been absent from the literature, both in terms of its membership of the SRB group and its relationship to corrosion.
文摘Song China was a period in which China experienced a great increase in its population.Concurrently,the Song dynasty also experienced a rise in the frequency of epidemics and two major wars with the Western Xia and Liao dynasties during the 1000s and 1040s.The consequences of these changes were exacerbated by the increased geographical mobility of certain social groups such as traders and examinees attending civil service examinations.Thus,casualties of wars,epidemics,or disease,especially of people whose families were far away and could not care for them were left without care and“their corpses often lay bare along the roads.”This new social environment created a need for general relief.The Northern Song government(960-1127 CE),especially during the reign of Emperor Huizong,established an innovative public health system to address this issue.The public health system included poorhouses,public hospitals,and pauper’s cemeteries.The first were more of charity organizations,whereas the latter two promoted public health by providing medical services for the poor and burial for those that nobody cared for.In terms of rationale behind these institutions,on the one hand,they constituted an attempt to get the poor and homeless off the streets while providing them relief or burial.On the other hand,it seems that Huizong’s deep concern with medicine propelled him to design and implement a comprehensive public health system oriented to prevent contagion and outbreak of epidemics.This article depicts the background,the organization,and the functions of the system.The article also discusses the conditions and reasons that gave rise to such a unique undertaking by the Northern Song government.
基金This research was supported by Basic Science Research Program of the National Research Foundation of Korea(NRF)in the Ministry of ScienceICT and Future Planning(NRF-2017R1A2B4005915)supported this research.
文摘We investigated the diversity and composition of microflora in feces of Lycopus lucidus Turcz.-fed mice.In addition,we evaluated the production of major cytokines(Interleukin-6 and-10)which are related to inflammation and fatty acid composition of several tissues.16S ribosomal DNA sequencing-based microbiome taxonomic profiling analysis was performed utilizing the EzBioCloud data base.Male mice fed on L.lucidus showed a significantly reduced number of lactic acid bacteria and coliform in the feces compared with the control group(p<0.05).16S rDNA sequencing analysis of fecal samples showed that L.lucidus supplementation decreased the community of harmful microflora(Enterobacteriaceae including Escherichia coli and Bacteroides sp.)in feces compared with the control group(p<0.05).There were no significant differences in mRNA expression of cytokine IL-6 and IL-10 between the control and L.lucidus fed groups.The fecal fatty acid composition in the L.lucidus group had percentages of 4:0,6:0,8:0 and 10:0 in the intestine but those short chain fatty acids were not detected in the control group.Our results showed that L.lucidus supplementation influenced gut environment by decreasing harmful microflora and increased the percentages of several short fatty acids.
文摘T.S.Eliot was a leader of the Modernist movement in poetry.His early poem The Love Song of J.Alfred Prufrock reveals the disappointment,loss,confusion and lack of faith that prevailed in Western society after the World WarⅠ.In this paper,the text of Love Song will be assessed from the perspective of intertextuality through the connection of the fantasy world and the disconnection of the real world.
基金Supported in part by 973 plan and NSF of Zhejiang Province of China(Gl999075105)
文摘We consider the weighted composition operators between Hardy spaces on the unit ball, and obtain some sufficient and necessary conditions of bounded or compact weighted composition operators. We also prove that the operator from H^1 to H^1 is compact if and only if it is weakly compact. Meanwhile, we get the analogue on the Bergman spaces.
基金This work was supported by NSF of China(11171203,11201280)New Teacher’s Fund for Doctor Stations,Ministry of Education(20114402120003)NSF of Guangdong Province(10151503101000025,S2011010004511,S2011040004131)
文摘We characterize the boundedness and compactness of weighted composition operators on weighted Dirichlet spaces in terms of Nevanlinna counting functions and Caxleson measure.
文摘In this paper, necessary and sufficient conditions for a closed range composition operator CФ on the general family of holomorphic function spaces F（p,q,s） and more generally on α-Besov type spaces F（p,αp-2,s） are given. We give a Carleson measure characterization on F （p, αp - 2, s） spaces, then we indicate how Carleson measures can be used to characterize boundedness and compactness of CФ on F（p,q,s） and F（p,αp- 2,s） spaces.
文摘By analyzing of the existing Web services,an ontology based on OWL is presented,which has rich semantic information,and the service description language OWL-S based on OWL is put forward. OWL-S through IOPE can describe services,can also combine the service,but the method of combination service is not automatic. So a method is presented by using Situation Calculus for automatic service composition based on the OWL-S model. Finally through the example analysis,the method of automatic service combination was validated.
基金Supported by the Special Fund for Public Projects of Zhejiang Province(2016C32073)Agriculture Project of Guizhou Province(20103047)International Cooperation Program of Zhejiang Academy of Agricultural Sciences
文摘The gastrointestinal tract, as the major digestive and absorbing organ, plays an important role in chicken growth. Numerous studies have been undertaken to investigate the gut bacterial community in broilers, but little is known about the archaeal community composition. To improve our understanding of the composition of intestinal archaea and the relationship between archaea and chicken growth, a feeding trial was conducted with 10 000 Ross 308 chicks in a commercial production facility under standard management. At 38 d of age, 15 birds with the highest body weight （ H group）, 15 birds with average body weight （ M group）, and 15 birds with the lowest body weight （ L group） were selected among 600 healthy male individuals. Cecal archaeal community was detected based on 16S rRNA gene sequencing. As a result, 3 321 229 archaeal sequences were clustered into 1 281 operational taxonomic units （OTUs） and classified into three phyla （Crenarchaeola, Euryarchaeota and Thaumarchaeota） and six known genera ： Natronomonas, Halolamina, Salinarchaeum, Cenarchaeum, Candidatus, Nitrosoarchaeum and Nitrososphaera. The alpha indices showed that, the richness of archaea in L group was the highest, and followed by that in M group and H group, while the archaea in H group were more diverse, followed by those in M group and L group. However, the archaeal composition in all chicken samples was similar, with the vast majority of the sequences belonging to Cenarchaeum, and only a few belonging to the other five genera.
文摘Thin films of ZnxCd1-xS have been prepared by electron beam evaporation of a mixture of ZnS & CdS powders. The films are deposited onto sodalime glass slides under similar conditions.The composition of the films is varied from CdS to ZnS (x=0 to 1). The films show a regular change in color from toner red to orange yellow as Zn concentration increases to maximum.These films are characterized for their optical, electricaI and structural properties. The bandgap value of ZnxCd1-xS films is found to vary linearIy from 2.20 eV to 3.44 eV with change in the x value from 0 to 1. The resistivity of these films is in the range of 171.0 Ωcm to 5.5× 106Ωcm for x=0~0.6. All the samples show cubic structure after annealing in air at 250℃ for 40 min.The lattice constant ao varies from 0.5884 nm to 0.54109 nm linearly.
文摘T.S.Eliot 's poem,"The Love Song of J.Alfred Prufrock",different and unusual,is difficult to interpret.T.S.Eliot rejects logic connection,thus his poems lack logic interpretation.He himself justifies himself by saying:he wrote it to want it to be difficult.The dissociation of sensibility,on the contrary,arouses the emotion of readers immediately.
