The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components.The seeds of Descurainia sophia(SDS)are utilized in China as a co...The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components.The seeds of Descurainia sophia(SDS)are utilized in China as a cough and asthma relieving agent.Herein,a dimension-enhanced integral approach,by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(UHPLC/IMQTOF-MS)and intelligent peak annotation,was developed to rapidly characterize the multicomponents from SDS.Good chromatographic separation was achieved within 38 min on a UPLC CSH C18(2.1×100 mm,1.7μm)column which was eluted by 0.1%formic acid in water(water phase)and acetonitrile(organic phase).Collision-induced dissociation-MS^(2)data were acquired by the data-independent high-definition MS^(E)(HDMS^(E))in both the negative and positive electrospray ionization modes.A major components knockout strategy was applied to improve the characterization of those minor ingredients by enhancing the injection volume.Moreover,a self-built chemistry library was established,which could be matched by the UNIFI software enabling automatic peak annotation of the obtained HDMS^(E)data.As a result of applying the intelligent peak annotation workflows and further confirmation process,a total of 53 compounds were identified or tentatively characterized from the SDS,including 29 flavonoids,one uridine derivative,four glucosides,one lignin,one phenolic compound,and 17 others.Notably,four-dimensional information related to the structure(e.g.,retention time,collision cross section,MS^(1)and MS^(2)data)was obtained for each component by the developed integral approach,and the results would greatly benefit the quality control of SDS.展开更多
BioPublisher,http://biopublisher.ca An international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all...BioPublisher,http://biopublisher.ca An international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professional peer review.As a member of Publishers International Linking Association,PILA,Bio Publisher fol-展开更多
Camelina and flixweed(sophia)seed protein isolates were prepared using both the conventional extraction and ultrasonic-assisted extraction methods at 40 kHz for 20 min,and their functional properties investigated.SDS-...Camelina and flixweed(sophia)seed protein isolates were prepared using both the conventional extraction and ultrasonic-assisted extraction methods at 40 kHz for 20 min,and their functional properties investigated.SDS-PAGE showed that both ultrasound-assisted and conventional extractions resulted in a similar protein profile of the extract.The application of ultrasound significantly improved protein extraction/content and functional properties(water holding capacity,oil absorption capacity,emulsifying foaming properties,and protein solubility)of camelina protein isolate and sophia protein isolate.The water-holding and oil absorption capacities of sophia protein isolate were markedly higher than those of camelina protein isolate.These results suggest that camelina protein isolate and sophia protein isolate may serve as natural functional ingredients in the food industry.展开更多
目的创建一种能够快速测定南葶苈子药材中芥子碱硫氰酸盐(sinapine thiocyanate,SH),槲皮素-3-O-β-D-葡萄糖-7-0-β-D-龙胆双糖苷(quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiobioside,QGG),异鼠李素-3-O-β-D-吡喃葡萄糖苷(isorha...目的创建一种能够快速测定南葶苈子药材中芥子碱硫氰酸盐(sinapine thiocyanate,SH),槲皮素-3-O-β-D-葡萄糖-7-0-β-D-龙胆双糖苷(quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiobioside,QGG),异鼠李素-3-O-β-D-吡喃葡萄糖苷(isorhamnetin-3-O-glucoside,IG),槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin3-O-β-D-glucopyranoside,QG)4种成分的方法。方法高效液相色谱法(high performance liquid chromatography,HPLC)测定南葶苈子样品中SH、QGG、IG、QG的含量,采用偏最小二乘法(partial least squares method,PLS)建立南葶苈子中SH、QGG、IG、QG的定量分析模型。结果所创建的QGG、QG、IG、SH的定量分析模型的内部交叉验证决定系数(R2)分别为0.9270、0.9266、0.8086、0.8289;校正均方差(root mean square error of calibration,RMSEC)分别为0.0100、0.00263、0.00313、0.111;验证均方差(root mean square error of prediction,RMSEP)分别为0.0139、0.00715、0.00395、0.0860。其中SH的特征光谱为9100~6100 cm-1;QGG的特征光谱为7300~5500 cm-1;IG的特征光谱为9500~6700 cm-1;QG的特征光谱为8200~5100 cm-1。结论利用傅里叶红外光谱建立的定量模型,能够对南葶苈子药材中QGG、QG、IG、SH的含量进行无损测定。此外通过近红外光谱PCA分析发现道地产地南葶苈子与非道地产地南葶苈子的差异较大,道地产地南葶苈子之间的差异较小。展开更多
目的 :研究南葶苈子中槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷的含量测定方法 ,建立南葶苈子药材质量标准。方法 :采用反相高效液相色谱法 ,以自制标准品作对照 ,对南葶苈子药材中主要有效成分槲皮素 3 O β D 葡萄糖 7 O β D...目的 :研究南葶苈子中槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷的含量测定方法 ,建立南葶苈子药材质量标准。方法 :采用反相高效液相色谱法 ,以自制标准品作对照 ,对南葶苈子药材中主要有效成分槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷进行了含量测定。 结果 :回收率为 99.78% ,RSD为 2 .4 %。结论 :该方法可用于南葶苈子药材的质量控制。展开更多
基金This work was financially supported by the National Key Research and Development Program of China(Grant No.2018YFC1704500)Tianjin Committee of Science and Technology of China(Grant No.21ZYJDJC00080)National Natural Science Foundation of China(Grant No.81872996).
文摘The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components.The seeds of Descurainia sophia(SDS)are utilized in China as a cough and asthma relieving agent.Herein,a dimension-enhanced integral approach,by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry(UHPLC/IMQTOF-MS)and intelligent peak annotation,was developed to rapidly characterize the multicomponents from SDS.Good chromatographic separation was achieved within 38 min on a UPLC CSH C18(2.1×100 mm,1.7μm)column which was eluted by 0.1%formic acid in water(water phase)and acetonitrile(organic phase).Collision-induced dissociation-MS^(2)data were acquired by the data-independent high-definition MS^(E)(HDMS^(E))in both the negative and positive electrospray ionization modes.A major components knockout strategy was applied to improve the characterization of those minor ingredients by enhancing the injection volume.Moreover,a self-built chemistry library was established,which could be matched by the UNIFI software enabling automatic peak annotation of the obtained HDMS^(E)data.As a result of applying the intelligent peak annotation workflows and further confirmation process,a total of 53 compounds were identified or tentatively characterized from the SDS,including 29 flavonoids,one uridine derivative,four glucosides,one lignin,one phenolic compound,and 17 others.Notably,four-dimensional information related to the structure(e.g.,retention time,collision cross section,MS^(1)and MS^(2)data)was obtained for each component by the developed integral approach,and the results would greatly benefit the quality control of SDS.
文摘BioPublisher,http://biopublisher.ca An international open access publishing pl atform based British Columbia of Canada,publishing scientific journals in the field of life science,now 38 journals have been launched,all are academic journals with professional peer review.As a member of Publishers International Linking Association,PILA,Bio Publisher fol-
文摘Camelina and flixweed(sophia)seed protein isolates were prepared using both the conventional extraction and ultrasonic-assisted extraction methods at 40 kHz for 20 min,and their functional properties investigated.SDS-PAGE showed that both ultrasound-assisted and conventional extractions resulted in a similar protein profile of the extract.The application of ultrasound significantly improved protein extraction/content and functional properties(water holding capacity,oil absorption capacity,emulsifying foaming properties,and protein solubility)of camelina protein isolate and sophia protein isolate.The water-holding and oil absorption capacities of sophia protein isolate were markedly higher than those of camelina protein isolate.These results suggest that camelina protein isolate and sophia protein isolate may serve as natural functional ingredients in the food industry.
文摘目的创建一种能够快速测定南葶苈子药材中芥子碱硫氰酸盐(sinapine thiocyanate,SH),槲皮素-3-O-β-D-葡萄糖-7-0-β-D-龙胆双糖苷(quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiobioside,QGG),异鼠李素-3-O-β-D-吡喃葡萄糖苷(isorhamnetin-3-O-glucoside,IG),槲皮素-3-O-β-D-吡喃葡萄糖苷(quercetin3-O-β-D-glucopyranoside,QG)4种成分的方法。方法高效液相色谱法(high performance liquid chromatography,HPLC)测定南葶苈子样品中SH、QGG、IG、QG的含量,采用偏最小二乘法(partial least squares method,PLS)建立南葶苈子中SH、QGG、IG、QG的定量分析模型。结果所创建的QGG、QG、IG、SH的定量分析模型的内部交叉验证决定系数(R2)分别为0.9270、0.9266、0.8086、0.8289;校正均方差(root mean square error of calibration,RMSEC)分别为0.0100、0.00263、0.00313、0.111;验证均方差(root mean square error of prediction,RMSEP)分别为0.0139、0.00715、0.00395、0.0860。其中SH的特征光谱为9100~6100 cm-1;QGG的特征光谱为7300~5500 cm-1;IG的特征光谱为9500~6700 cm-1;QG的特征光谱为8200~5100 cm-1。结论利用傅里叶红外光谱建立的定量模型,能够对南葶苈子药材中QGG、QG、IG、SH的含量进行无损测定。此外通过近红外光谱PCA分析发现道地产地南葶苈子与非道地产地南葶苈子的差异较大,道地产地南葶苈子之间的差异较小。
文摘目的 :研究南葶苈子中槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷的含量测定方法 ,建立南葶苈子药材质量标准。方法 :采用反相高效液相色谱法 ,以自制标准品作对照 ,对南葶苈子药材中主要有效成分槲皮素 3 O β D 葡萄糖 7 O β D 龙胆双糖苷进行了含量测定。 结果 :回收率为 99.78% ,RSD为 2 .4 %。结论 :该方法可用于南葶苈子药材的质量控制。
