The chemical structure of an exopolysaccharide (EPS) produced by Sorangium cellulosum NUST06 was investigated. The EPS was found to have molecular masses greater than 2?106 Da, and consisted of D-glucose, D-mannose an...The chemical structure of an exopolysaccharide (EPS) produced by Sorangium cellulosum NUST06 was investigated. The EPS was found to have molecular masses greater than 2?106 Da, and consisted of D-glucose, D-mannose and D-glucuronic acid in a ratio of 5:4:1. The analytical results of methylation and nuclear magnetic resonance spectra suggested the EPS consist of following tetrasaccharide repeating units: 4) -b-D-Glup (1 4)- b-D-Glup (1 4) -b-D-Glup (1 a-D-GluAp (1 6) a-D-manp (1 6)-a-D-manp (1 6) (6) (4) (4) ↑ ↑ ↑ Me Ac Ac展开更多
Four natural epothilone analogues, including two 14-membered epothilones L （1）, L1 （2） and two epoxide opened epothilones A （3） and B （4）, were isolated from the extract of fermentation adsorber resin of Soran...Four natural epothilone analogues, including two 14-membered epothilones L （1）, L1 （2） and two epoxide opened epothilones A （3） and B （4）, were isolated from the extract of fermentation adsorber resin of Sorangium cellulosum strain So0157-2. Their structures were elucidated on the basis of comprehensive NMR and MS data. They are all isolated from the wild type S. cellulosum strain So0157-2 as natural products for the first time.展开更多
采用滤纸片富集法对采自广东省信宜的土壤样品进行了粘细菌的分离和纯化，共获得6个菌株（Soce M1～So ce M6）。利用体视显微镜、倒置显微镜和扫描电镜对这些菌株的子实体、菌落及营养细胞等进行了形态学的观察和特征描述。同时，还测...采用滤纸片富集法对采自广东省信宜的土壤样品进行了粘细菌的分离和纯化，共获得6个菌株（Soce M1～So ce M6）。利用体视显微镜、倒置显微镜和扫描电镜对这些菌株的子实体、菌落及营养细胞等进行了形态学的观察和特征描述。同时，还测定了6个菌株的16SrRNA基因序列，应用blast程序与GenBank中已知的纤维堆囊菌及其他粘细菌序列进行比对，并采用邻位相连法（Neighbor-Joining，NJ）构建了分离菌株与相关菌株的系统发育树。结果表明：6个菌株均为纤维堆囊菌（Sorangium cellulose），但这些菌株在形态特征和16S。RNA基因进化距离上存在着一定的差异，表现出纤维堆囊菌菌株的形态多样性和遗传多样性。展开更多
文摘The chemical structure of an exopolysaccharide (EPS) produced by Sorangium cellulosum NUST06 was investigated. The EPS was found to have molecular masses greater than 2?106 Da, and consisted of D-glucose, D-mannose and D-glucuronic acid in a ratio of 5:4:1. The analytical results of methylation and nuclear magnetic resonance spectra suggested the EPS consist of following tetrasaccharide repeating units: 4) -b-D-Glup (1 4)- b-D-Glup (1 4) -b-D-Glup (1 a-D-GluAp (1 6) a-D-manp (1 6)-a-D-manp (1 6) (6) (4) (4) ↑ ↑ ↑ Me Ac Ac
基金supported by Key Program of International Cooperation, Ministry of Science and Technology of China (No.2007DFA30970)the 863 Program of China (No. 20060102Z1083)~~
基金National Natural Science Fund for Distinguished Young Scholars (Grant No.30325044,30825001)the 863 Program of China (Grant No.20060102Z1083)
文摘Four natural epothilone analogues, including two 14-membered epothilones L （1）, L1 （2） and two epoxide opened epothilones A （3） and B （4）, were isolated from the extract of fermentation adsorber resin of Sorangium cellulosum strain So0157-2. Their structures were elucidated on the basis of comprehensive NMR and MS data. They are all isolated from the wild type S. cellulosum strain So0157-2 as natural products for the first time.
文摘采用滤纸片富集法对采自广东省信宜的土壤样品进行了粘细菌的分离和纯化，共获得6个菌株（Soce M1～So ce M6）。利用体视显微镜、倒置显微镜和扫描电镜对这些菌株的子实体、菌落及营养细胞等进行了形态学的观察和特征描述。同时，还测定了6个菌株的16SrRNA基因序列，应用blast程序与GenBank中已知的纤维堆囊菌及其他粘细菌序列进行比对，并采用邻位相连法（Neighbor-Joining，NJ）构建了分离菌株与相关菌株的系统发育树。结果表明：6个菌株均为纤维堆囊菌（Sorangium cellulose），但这些菌株在形态特征和16S。RNA基因进化距离上存在着一定的差异，表现出纤维堆囊菌菌株的形态多样性和遗传多样性。
文摘以纤维堆囊菌(Sorangium cellulosum)So F5-76为诱变出发菌株,经紫外(UV)和亚硝基胍(NTG)复合诱变和筛选,获得一株高产突变菌株So F5-H23,其发酵产埃博霉素B的量可达79.83 mg/L,比出发菌株提高了1.27倍。通过Plackett-Burman实验和响应面分析法对纤维堆囊菌产埃博霉素B的发酵工艺进行优化。得到最佳发酵工艺为:马铃薯淀粉4.8 g/L,葡萄糖0.5 g/L,脱脂奶粉2.3 g/L,豆饼粉2 g/L,七水硫酸镁2 g/L,无水氯化钙2 g/L,EDTA-Fe3+2 m L/L,微量元素(TE)0.5 m L/L,吸附树脂2%,培养基初始p H 7.4,装液量50 m L,接种体积分数8%,温度30℃。在此最优条件下埃博霉素B产量为108.67 mg/L,比优化前提高了36.13%,此产量是国内外报道的埃博霉素最高产量。
