A study was conducted to determine the effects of electrostatic field （ESF） treatment on seed germination and seedling growth of Sorbus pohuashanesis. The experiments were arranged by uniform design computed by the ...A study was conducted to determine the effects of electrostatic field （ESF） treatment on seed germination and seedling growth of Sorbus pohuashanesis. The experiments were arranged by uniform design computed by the Data Processing System （DPS）, including three levels of seeds soaking time, four levels of ESF intensity and four levels of ESF treatment time, with 12 treatments. Ten seeds were used in each treatment with three replicates. Seed vigor, seed germinating ability, emergence rate of seedling, survival rate of seedling, and seedling height and diameter, as well as the change in activities of superoxide dismutase （SOD）, soluble protein contents, total chlorophyll contents, soluble total sugar contents in leaves of S. pohuashanensis seedlings were measured after ESF treatments. The experiment results show that ESF treatment could improve the water absorption ability of dry seeds of S. pohuashanensis, resulting in fast germination at room temperature under light conditions. Combined treatment of ESF with cold stratification could increase seed germination percentage significantly （to 42.20%）, promote seedling height growth, affect leaf SOD activity, and could raise contents of total chlorophyll, soluble protein, and total soluble sugar in leaves. Seed soaking time had a significant effect on seed relative electroconductivity, seed germination under light, SOD activity, soluble protein content and total soluble sugar content of seedling leaves. ESF intensity exerted a moderate effect on these indexes. ESF treatment time only had significant effect on total chlorophyll contents, no evident effect on other indexes.展开更多
We characterized the effects of KNO3 pretreatment and germination temperature on dormancy breaking and germination of mature mountain ash seeds. Seeds treated with KNO3 and germinated at 25 ℃ followed by 5 ℃ had sig...We characterized the effects of KNO3 pretreatment and germination temperature on dormancy breaking and germination of mature mountain ash seeds. Seeds treated with KNO3 and germinated at 25 ℃ followed by 5 ℃ had significantly higher germination percentages and germination potentials （51% and 49%, respectively）, compared with controls. These treated seeds also exhibited reduced germination initiation times （minimum of 48 days）, and elevated germination rate indices （up to 97）. The germination of seeds subjected to long-term cold storage （2 years at 0-5 ℃） was also significantly improved by 3 days of 4% KNO3 pretreatment before germinating under a variable temperature regimen （5 ℃ followed by 25 ℃, and followed by 5 ℃）. Germination percentages and germination potentials for these cold-stored seeds reached 67% and 54%, respectively, and the germination rate index increased to 126.99. Pretreatment of mountain ash seeds with KNO3 represents a practical, effective, and pollution-free method for improving germination, and can be implemented easily within a variety of nursery settings.展开更多
基金supported by the Key Technologies R&D Program of China during 2006–2010 (2006BAD03A04)
文摘A study was conducted to determine the effects of electrostatic field （ESF） treatment on seed germination and seedling growth of Sorbus pohuashanesis. The experiments were arranged by uniform design computed by the Data Processing System （DPS）, including three levels of seeds soaking time, four levels of ESF intensity and four levels of ESF treatment time, with 12 treatments. Ten seeds were used in each treatment with three replicates. Seed vigor, seed germinating ability, emergence rate of seedling, survival rate of seedling, and seedling height and diameter, as well as the change in activities of superoxide dismutase （SOD）, soluble protein contents, total chlorophyll contents, soluble total sugar contents in leaves of S. pohuashanensis seedlings were measured after ESF treatments. The experiment results show that ESF treatment could improve the water absorption ability of dry seeds of S. pohuashanensis, resulting in fast germination at room temperature under light conditions. Combined treatment of ESF with cold stratification could increase seed germination percentage significantly （to 42.20%）, promote seedling height growth, affect leaf SOD activity, and could raise contents of total chlorophyll, soluble protein, and total soluble sugar in leaves. Seed soaking time had a significant effect on seed relative electroconductivity, seed germination under light, SOD activity, soluble protein content and total soluble sugar content of seedling leaves. ESF intensity exerted a moderate effect on these indexes. ESF treatment time only had significant effect on total chlorophyll contents, no evident effect on other indexes.
文摘We characterized the effects of KNO3 pretreatment and germination temperature on dormancy breaking and germination of mature mountain ash seeds. Seeds treated with KNO3 and germinated at 25 ℃ followed by 5 ℃ had significantly higher germination percentages and germination potentials （51% and 49%, respectively）, compared with controls. These treated seeds also exhibited reduced germination initiation times （minimum of 48 days）, and elevated germination rate indices （up to 97）. The germination of seeds subjected to long-term cold storage （2 years at 0-5 ℃） was also significantly improved by 3 days of 4% KNO3 pretreatment before germinating under a variable temperature regimen （5 ℃ followed by 25 ℃, and followed by 5 ℃）. Germination percentages and germination potentials for these cold-stored seeds reached 67% and 54%, respectively, and the germination rate index increased to 126.99. Pretreatment of mountain ash seeds with KNO3 represents a practical, effective, and pollution-free method for improving germination, and can be implemented easily within a variety of nursery settings.
