Antibacterial activity of marine bacteria isolated from sponge Xestospongia testudinaria from Sorong,Papua

Objective:To explore secondary metabolite of bacteria-associated Xestospongia testudinaria from Tanjung Kasuari,Sorong,Papua.Methods:The antimicrobial activities of extracts against two Gram-positive bacteria(Staphylococcus aureus) and three Gram-negative bacteria(Pseudomonas aeruginosa,Eschericia coli and Salmonella typhi) were determined by disk diffusion dilution method.Results:The test showed that of 15 isolates of symbiont bacteria,6 isolates were successfully isolated and coded,namely,Xp 4.1,Xp 4.2,Xp 4.3,Xp 4.4,Xp 4.5 and Xp 4.6.Of the six bacterial isolates,isolated Xp 4.2 was found to have more powerful antibacterial activity than any other isolates of symbiont bacteria.Antibacterial activity assay for the n-hexane soluble fractions,ethyl-acetate soluble fractions,and n-buthanol soluble fractions revealed more powerful anti-bacterial activity than any other soluble fractions.Phytochemical screening showed alkaloid and steroid/triterpenoid,while identification for isolate of Xp 4.2 bacterial showed bacteria.Conclusions:Metabolites of bacterial associated with marine sponge Xestospongia testudinaria promise to be developed into antibacterial agents.

Facies and Sequence Stratigraphy Analyses of Klasaman Formation in the “X Well”, Northern Salawati Basin, Papua, Indonesia

The “X Well” is located in the Salawati Basin which is part of Western Papua. The interval of study is the Klasaman Formation that is characterized by thick sediment intercalated sandstones and shales. The movement of Sorong Fault strongly controlled the sedimentation of this formation. This study is very important to give a contribution for developing exploration prospect by predicting reservoir distribution since localized facies change may mark the distribution of sandstones. The current study aims: 1) to define the age of Klasaman Formation;2) to define the vertical facies;3) to define the sequence stratigraphy;and 4) to predict the lateral change of facies. Five nannofossil datum planes are found throughout the interval and cover the age Late Miocene to Early Pliocene. LO Sphenolithus abies points the age 3.65 Ma, LO Reticulofenestra pseudoumbilicus points the age 3.79 Ma, FO Pseudoemiliania lacunosa points the age 4 Ma, LO Discoaster quinqueramus and LO Discoaster berrgrenii point the age 5.59 Ma and Top of small Reticulofenestra Interval points the age 7.167 Ma. They define the NN11 up to NN16 Martini’s zonation. The age of Klasaman Formation is known as the uppermost Late Miocene to the Early Pliocene. Four facies can be identified, namely Upper Shoreface, Lower Shoreface, Offshore Transition and Offshore. The upper shoreface facies is characterized by thick and clean sandstones, about 2.41 - 20.64 meters thick, while the lower shoreface facies consists of predominantly very fine to fine-grained sandstones intercalated by siltstones and claystones. The offshore transition facies is marked by thick claystones and siltstones intercalated with thin sandstones, whilst the offshore facies is marked by thick laminated claystones. Nine Parasequences 4th order can be identified within study interval, namely Progradational Parasequence-1 to 2, Retrogradational Parasequence-3, Progradational Parasequence-4 to 6, Retrogradational Parasequence-7 to 9. They are bounded by Sequence Boundary-1 to 8. The upper shoreface sandstones of Parasequence-4 to 9 can be potential reservoir rocks in the Klasaman Formation since the sandstones are very thick and clean. The sediments of Klasaman Formation have been sourced from elevated highland in the northeast area, so the upper shoreface sandstones have changed become the offshore claystones from northeast to southwest. High sedimentation rate and rapid subsidence of the basin mark the deposition of Klasaman Formation especially during the deposition of Parasequence-6 to 9 as a result of Sorong Fault tectonics.