基于SYBR Green I荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法。采用不同稀释倍数的SYBR Green I荧光染料做DNA的标准曲线,确定SYBR Green I荧光染料的最佳稀释倍数。比较荧光法和Southern blotting杂交法定量DN...基于SYBR Green I荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法。采用不同稀释倍数的SYBR Green I荧光染料做DNA的标准曲线,确定SYBR Green I荧光染料的最佳稀释倍数。比较荧光法和Southern blotting杂交法定量DNA。结果表明,SYBR Green I荧光染料稀释倍数为1∶10 000时线性范围广,线性关系好(R2=0.9999);荧光法和Southern blotting杂交法可以相互印证。由此可知,SYBR Green I荧光法定量DNA是一种快速、稳定的痕量DNA检测方法。展开更多
Danghara,a major food production area in southern Tajikistan,is currently suffering from the impact of rapid climate change and intensive human activities.Assessing the future impact of climate change on crop water re...Danghara,a major food production area in southern Tajikistan,is currently suffering from the impact of rapid climate change and intensive human activities.Assessing the future impact of climate change on crop water requirements(CWRs)for the current growing period and defining the optimal sowing date to reduce future crop water demand are essential for local/regional water and food planning.Therefore,this study attempted to analyze possible future climate change effects on the water requirements of major crops using the statistical downscaling method in the Danghara District to simulate the future temperature and precipitation for two future periods(2021-2050 and 2051-2080),under three representative concentration pathways(RCP2.6,RCP4.5,and RCP8.5)according to the CanESM2 global climate model.The water footprint(WFP)of major crops was calculated as a measure of their CWRs.The increased projection of precipitation and temperature probably caused an increase in the main crop’s WFP for the current growing period,which was mainly due to the green water(GW)component in the long term and a decrease in the blue water(BW)component during the second future period,except for cotton,where all components were predicted to remain stable.Under three scenarios for the two future potato and winter wheat decreased from 5.7%to 4.8%and 3.4%to 2.2%,respectively.Although the WFP of cotton demonstrated a stable increase,according to the optimal sowing date,adecrease in irrigation demand or Bw was expected.The results of our study might be useful fordeveloping a new strategy related to irrigation systems and could help to find a balance betweenwater and food for environmental water demands and human use.展开更多
Harmful algal blooms(HABs)in the Southern Yellow Sea(SYS)have shown a trend of increasing diversity and detrimental ef fects.While the Bohai Sea,East China Sea,and South China Sea have experienced a high incidence of ...Harmful algal blooms(HABs)in the Southern Yellow Sea(SYS)have shown a trend of increasing diversity and detrimental ef fects.While the Bohai Sea,East China Sea,and South China Sea have experienced a high incidence of HABs since the 1980s,the Yellow Sea provides a relatively healthy ecological environment in which fewer HABs have been documented before the 21s t century.Yet largescale blooms of the green macroalga Ulva prolifera(so-called“green tides”)have occurred annually since 2007 in the Yellow Sea.Six people were poisoned and one person died in Lianyungang in 2008 due to ingestion of algal toxins.Moreover,the Yellow Sea experienced co-occurrence of harmful red tides,green tides,and golden tides in 2017.This combination of events,rare worldwide,indicates the potential for further deterioration of the marine environment in the Yellow Sea,which may be related to climate change,aquaculture,and other human activities.Using the SYS as an example,we collected data of the frequency and scale of HABs over the years,as well as that of marine algal toxins,and analyzed the trend in the diversity of HABs in the SYS,to explore the causes and impacts of HABs,as well as the interrelationships among dif ferent types of HABs,including harmful red tides,green tides,and golden tides.We also attempted to improve our understanding of HAB evolution under the influence of global climate change and intensified human activities.展开更多
文摘为了分析南方型黑杨生长性状、木材性状对其湿心材形成的影响程度,于2023年11月份,在岳阳市君山区丁字堤南方型黑杨试验林,选取13年生的8个南方型黑杨无性系为研究对象,每个无性系选取6株样株,用9 mm生长锥在树高1.3 m处钻取木样,分别测量木芯长度、湿心材长度,并称量质量;依据试验设计,测定样木的胸径、树高、材积、树液pH、生材含水量、气干材含水量、木材基本密度;运用相关分析法、通径分析法,计算各性状对湿心材比例的直接影响、间接影响、决定系数,分析南方型黑杨无性系生长性状、木材性状的7个性状指标对湿心材比例的影响。结果表明:8个无性系间,湿心材比例、胸径、树高、材积、生材含水量存在极显著差异。树液pH和生材含水量,与湿心材比例存在极显著正相关,气干材含水量与湿心材比例存在显著正相关。通径分析表明,生材含水量(W r)、树液pH、气干材含水量(W a)是影响湿心材比例(R(wh))的重要因子,通过多元回归分析建立3个性状与湿心材比例的多元回归方程R(wh)=-0.995+0.006pH+0.075 W r+0.005 W a;其中,生材含水量对湿心材比例的直接影响(0.522)和单独决定系数大小表明,生材含水量对湿心材比例影响最大。因此,生材含水量大小可以作为选育南方型黑杨低湿心材选育时的评定指标。
文摘基于SYBR Green I荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法。采用不同稀释倍数的SYBR Green I荧光染料做DNA的标准曲线,确定SYBR Green I荧光染料的最佳稀释倍数。比较荧光法和Southern blotting杂交法定量DNA。结果表明,SYBR Green I荧光染料稀释倍数为1∶10 000时线性范围广,线性关系好(R2=0.9999);荧光法和Southern blotting杂交法可以相互印证。由此可知,SYBR Green I荧光法定量DNA是一种快速、稳定的痕量DNA检测方法。
基金supported by the National Natural Science Foundation of China(41761144079)the State's Key Project of Researchand Development Plan(2017YFC404501)+4 种基金the CAS Interdisplinary Imnnovation Team(JCTD201920)the Strategic Priority Research Program of the Chinese Academy of Sciences,the Pan-Third Pole Environment Study for a Green Silk Road(XDA20060303)the International Partneship Program of the Chinese Aademy of Sciences(131551KYSB20160002)the CAS Research Center for Ecologyand Environment of Central Asia(Y934031)the Regional Collaborative Innovation Project of Xinjiang Uygur Autonomous Region(2020E01010).
文摘Danghara,a major food production area in southern Tajikistan,is currently suffering from the impact of rapid climate change and intensive human activities.Assessing the future impact of climate change on crop water requirements(CWRs)for the current growing period and defining the optimal sowing date to reduce future crop water demand are essential for local/regional water and food planning.Therefore,this study attempted to analyze possible future climate change effects on the water requirements of major crops using the statistical downscaling method in the Danghara District to simulate the future temperature and precipitation for two future periods(2021-2050 and 2051-2080),under three representative concentration pathways(RCP2.6,RCP4.5,and RCP8.5)according to the CanESM2 global climate model.The water footprint(WFP)of major crops was calculated as a measure of their CWRs.The increased projection of precipitation and temperature probably caused an increase in the main crop’s WFP for the current growing period,which was mainly due to the green water(GW)component in the long term and a decrease in the blue water(BW)component during the second future period,except for cotton,where all components were predicted to remain stable.Under three scenarios for the two future potato and winter wheat decreased from 5.7%to 4.8%and 3.4%to 2.2%,respectively.Although the WFP of cotton demonstrated a stable increase,according to the optimal sowing date,adecrease in irrigation demand or Bw was expected.The results of our study might be useful fordeveloping a new strategy related to irrigation systems and could help to find a balance betweenwater and food for environmental water demands and human use.
基金Supported by the Science&Technology Basic Resources Investigation Program of China(No.2018FY100200)the Key Deployment Project of Centre for Ocean Mega-Research of Science,Chinese Academy of Science(No.COMS2019Q05)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050302)the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0504)the Sino-Australian Centre for Healthy Coasts(No.2016YFE0101500)。
文摘Harmful algal blooms(HABs)in the Southern Yellow Sea(SYS)have shown a trend of increasing diversity and detrimental ef fects.While the Bohai Sea,East China Sea,and South China Sea have experienced a high incidence of HABs since the 1980s,the Yellow Sea provides a relatively healthy ecological environment in which fewer HABs have been documented before the 21s t century.Yet largescale blooms of the green macroalga Ulva prolifera(so-called“green tides”)have occurred annually since 2007 in the Yellow Sea.Six people were poisoned and one person died in Lianyungang in 2008 due to ingestion of algal toxins.Moreover,the Yellow Sea experienced co-occurrence of harmful red tides,green tides,and golden tides in 2017.This combination of events,rare worldwide,indicates the potential for further deterioration of the marine environment in the Yellow Sea,which may be related to climate change,aquaculture,and other human activities.Using the SYS as an example,we collected data of the frequency and scale of HABs over the years,as well as that of marine algal toxins,and analyzed the trend in the diversity of HABs in the SYS,to explore the causes and impacts of HABs,as well as the interrelationships among dif ferent types of HABs,including harmful red tides,green tides,and golden tides.We also attempted to improve our understanding of HAB evolution under the influence of global climate change and intensified human activities.
