Southern blot analysis was conducted in 15 patients by using a 1.2 Kb Hind 1 Ⅲ/Bgl Ⅱfragment from the 3'end of the bcr region and a 2.0 Kb Bgl Ⅱ/Hind Ⅲ fragment from the 5' end of the bcr as probes.Of the ...Southern blot analysis was conducted in 15 patients by using a 1.2 Kb Hind 1 Ⅲ/Bgl Ⅱfragment from the 3'end of the bcr region and a 2.0 Kb Bgl Ⅱ/Hind Ⅲ fragment from the 5' end of the bcr as probes.Of the 15 patients in our group,11 with chronic myelocytic leukemia (CML),3 with Ph-negative acute lymphocytic leukenma(ALL).one with myelodysplastic syndrome ZMDS).Bcr rearrangements were detected in 9 patients with CML and were negative in the rest of the patients.The results showed that the identification of bcr rearrangement was very important in the diagnosis of Ph-positive leukemias.展开更多
DNA from 36 patients with chronic myelogenous leukemia (CML) at various clinical stages and 6 cases of acute leukemia was investigated for alterations of the p53 gene by Southern blot analysis.Rearrangements of the p5...DNA from 36 patients with chronic myelogenous leukemia (CML) at various clinical stages and 6 cases of acute leukemia was investigated for alterations of the p53 gene by Southern blot analysis.Rearrangements of the p53 gene were seen in 3 of 12 (25.00%) cases of blast crisis and accelerated phase (AP) of CML and in only one of 18 chronic phrase (CP),just as has been reported previously. Meanwhile,by restriction fragment length polymorphism (RFLP) analysis the Bgl II site polymorphism in the p53 gene was also found. The frequency in Chinese people detected here was 0.392,which was strikingly higher than that in some other countries(P<0. 001).These results suggested that the alterations of the p53 gene, for example,p53 rearrangements,were probably responsible for the progression of BC in some CML patients, and that the frequency of Bgl II polymorphism in the p53 gene might be related to the population distribution.展开更多
文摘Southern blot analysis was conducted in 15 patients by using a 1.2 Kb Hind 1 Ⅲ/Bgl Ⅱfragment from the 3'end of the bcr region and a 2.0 Kb Bgl Ⅱ/Hind Ⅲ fragment from the 5' end of the bcr as probes.Of the 15 patients in our group,11 with chronic myelocytic leukemia (CML),3 with Ph-negative acute lymphocytic leukenma(ALL).one with myelodysplastic syndrome ZMDS).Bcr rearrangements were detected in 9 patients with CML and were negative in the rest of the patients.The results showed that the identification of bcr rearrangement was very important in the diagnosis of Ph-positive leukemias.
文摘DNA from 36 patients with chronic myelogenous leukemia (CML) at various clinical stages and 6 cases of acute leukemia was investigated for alterations of the p53 gene by Southern blot analysis.Rearrangements of the p53 gene were seen in 3 of 12 (25.00%) cases of blast crisis and accelerated phase (AP) of CML and in only one of 18 chronic phrase (CP),just as has been reported previously. Meanwhile,by restriction fragment length polymorphism (RFLP) analysis the Bgl II site polymorphism in the p53 gene was also found. The frequency in Chinese people detected here was 0.392,which was strikingly higher than that in some other countries(P<0. 001).These results suggested that the alterations of the p53 gene, for example,p53 rearrangements,were probably responsible for the progression of BC in some CML patients, and that the frequency of Bgl II polymorphism in the p53 gene might be related to the population distribution.
