Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus ...Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.展开更多
Using the primers which specially amplify the conservative motif of Human SRY gene, we studied the PCR amplification of Sox genes in genomic DNA of two species of mud loach: Misgurnus anguillicaudatus and Paramis...Using the primers which specially amplify the conservative motif of Human SRY gene, we studied the PCR amplification of Sox genes in genomic DNA of two species of mud loach: Misgurnus anguillicaudatus and Paramisgurnus dabryanus. Four bands with the length of 200,550,940 and 1000 bp respectively, were presented in the PCR products of Misgurnus anguillicaudatus. Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgurnus dabryanus. Southern blotting results indicated that the 200 and 550 bp bands are specially positive. There is no difference between male and female individuals as well as between these two species.展开更多
文摘Using the primers which specially amplify the conservative motif of Human SRYgene,we stud ied the PCR amplification of Sox genes in genomic DNA of two species of mud loach Misgurus anguillicau-datus and Paramisgurnus dabryanus.Four bands with the length of 200,550,940 and 1000 bp respectively,were presented in the PCR products of Misgumus anguillicaudatus.Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgumus dabryamus.Southern blotting results indicated that the 200 and 550 bp bands are specially positive.There is no difference between male and female individuals as well as between these two species.
文摘Using the primers which specially amplify the conservative motif of Human SRY gene, we studied the PCR amplification of Sox genes in genomic DNA of two species of mud loach: Misgurnus anguillicaudatus and Paramisgurnus dabryanus. Four bands with the length of 200,550,940 and 1000 bp respectively, were presented in the PCR products of Misgurnus anguillicaudatus. Three bands with the length of 200,550 and 900 bp were presented in that of Paramisgurnus dabryanus. Southern blotting results indicated that the 200 and 550 bp bands are specially positive. There is no difference between male and female individuals as well as between these two species.
