The SoxC transcription factors （Sox4, Sox11, and Sox12） play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration re...The SoxC transcription factors （Sox4, Sox11, and Sox12） play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration remain elusive. In this study, we investigated the expression and possible functions of the SoxC genes after retinal and optic nerve injury in adult zebrafish. We found that among the five SoxC members, Soxllb was strongly induced in BrdU-positive cells in the inner nuclear layer （INL） after retinal injury, and morpholino-mediated Soxllb-knockdown significantly reduced the number of proliferating cells in the INL at 4 days post-injury. After optic nerve lesion, both Soxl la and Soxl lb were strongly expressed in retinal ganglion cells （RGCs）, and knockdown of both Soxl la and Soxllb inhibited RGC axon regrowth in retinal explants. Our study thus uncovered a novel expression pattern of SoxC family genes after retinal and optic nerve injury, and suggests that they have important functions during retinal and optic nerve regeneration.展开更多
基金supported by grants from National Natural Science Foundation of China(31401234)Natural Science Foundation of Jiangsu Province,China(BK20140428)+1 种基金the Basic Research Program of Education Department of Jiangsu Province,China(14KJB180019)the Science and Technology Project of Nantong Municipality,Jiangsu Province,China(MS22015002)
文摘The SoxC transcription factors （Sox4, Sox11, and Sox12） play important roles in the development of the vertebrate eye and retina. However, their expression and function during retinal and optic nerve regeneration remain elusive. In this study, we investigated the expression and possible functions of the SoxC genes after retinal and optic nerve injury in adult zebrafish. We found that among the five SoxC members, Soxllb was strongly induced in BrdU-positive cells in the inner nuclear layer （INL） after retinal injury, and morpholino-mediated Soxllb-knockdown significantly reduced the number of proliferating cells in the INL at 4 days post-injury. After optic nerve lesion, both Soxl la and Soxl lb were strongly expressed in retinal ganglion cells （RGCs）, and knockdown of both Soxl la and Soxllb inhibited RGC axon regrowth in retinal explants. Our study thus uncovered a novel expression pattern of SoxC family genes after retinal and optic nerve injury, and suggests that they have important functions during retinal and optic nerve regeneration.
