Biochemical determination of lipid content in hepatic steatosis by the Soxtec method

AIM:To establish a quantitative method to measure the amount of lipids.METHODS:The livers of 53 male Wistar rats(225 g) with different degrees of hepatic steatosis were studied.This model of hepatic steatosis was based on a high carbohydrate,fat-free modified diet.Biopsies were classified into four grades depending on fat accumulation,using the Kleiner and Brunt classification.Total fat was studied by the Soxtec method(SoxtecTM 2050 Auto Fat Extraction System),and agreement between both assays was assessed by calculating theκ coefficient.RESULTS:According to the histological classification,38% of rats presented grade 0,21% grade 1,22% grade 2 and 20% grade 3.The amount of fat per 100 g tissue was 2.60±0.64 g for grade 0,3.87±1.59 g for grade 1,5.82±1.37 g for grade 2 and 8.68± 2.30 g for grade 3.Statistically significant differences were found between the mean values for each of the histological grades(P<0.05).The correlation for the quantification of fat in the liver between both assays was moderate(κ=0.60).CONCLUSION:The biochemical quantification of fat in liver tissue by the Soxtec method was correlated with the histological classification,although the agreement between the two tests was only moderate.

植物油不溶性杂质含量测定方法的改进

针对现有国标GB/T 15688—2008方法在测定植物油不溶性杂质含量时,滤纸表面油渍洗涤步骤耗时长,测定效率低的缺点,研究用Soxtec索氏浸提装置的淋洗方式替代国标规定的滤纸表面油渍冲洗方法,并对两种方法进行比较。结果表明:采用Soxtec淋洗方式结果准确,与标准方法相比,连续处理6个样品可节省操作时间至少5 h,且具有重现性好,节约试剂和减少试剂污染等优点。对于未经脱色的深色油脂,Soxtec淋洗也能充分洗掉滤纸上残留的色素。因此,同时分析多个油脂样品不溶性杂质含量时,采用Soxtec索氏浸提装置淋洗方式效率更高。