Soybean agglutinin(SBA)is an important anti-nutritional factor in soybean.SBA can induce animal growth inhibition,cause pathological changes of intestinal tissue,and decrease in the immune system functioning.Recently,...Soybean agglutinin(SBA)is an important anti-nutritional factor in soybean.SBA can induce animal growth inhibition,cause pathological changes of intestinal tissue,and decrease in the immune system functioning.Recently,a great deal of research has been done on the effects of SBA on cell morphology,division,apoptosis,autophagy,as well as the correlated signal transduction pathway.This review mainly covers the chemical and biological characteristics of SBA,describes the multifaceted aspects of SBA anti-nutritional functions,and highlights the possible cellular and molecular mechanism of anti-nutritional effects of SBA.This review has important implications for the prevention and treatment of SBA-induced diseases,drug development,processing techniques of plant products,prevention of food-borne toxins,as well as human and animal health protection.展开更多
A novel and efficient method for purification of soybean agglutinin (SBA) from soybean was reported. The method was characterized by selective extraction of SBA from soybean homogenate with barbiturate buffer (pH 6 2)...A novel and efficient method for purification of soybean agglutinin (SBA) from soybean was reported. The method was characterized by selective extraction of SBA from soybean homogenate with barbiturate buffer (pH 6 2), removal of impurity by hydroxyapatite, and the final purification of SBA by guaran affinity chromatography. The purified SBA showed a single band of 27 5 kD by SDS PAGE. The lowest concentration of SBA that caused agglutination of the rabbit red blood cells was 0 31 mg/L. Agglutination of different cancer cell lines by the purified SBA was examined. Strong agglutination of the human nasopharyngeal CNE cells, mouse Lewis lung carcinoma cells, and rat mammary adenocarcinoma R3230AC cells was observed. However, SBA could not agglutinate the human hepatocellular carcinoma BEL 7402 cells, suggesting that unlike the above mentioned three cell lines, the BEL 7402 cells may not express N acetylgalactosamine (GalNAc) or galactose (Gal) residues in significant amount at the non reducing terminals of their cell surface glycans.展开更多
基金supported by the National NaturalScienceFoundationofChina(No.31802093)Fund Project of Jilin Agricultural University(2019019).
文摘Soybean agglutinin(SBA)is an important anti-nutritional factor in soybean.SBA can induce animal growth inhibition,cause pathological changes of intestinal tissue,and decrease in the immune system functioning.Recently,a great deal of research has been done on the effects of SBA on cell morphology,division,apoptosis,autophagy,as well as the correlated signal transduction pathway.This review mainly covers the chemical and biological characteristics of SBA,describes the multifaceted aspects of SBA anti-nutritional functions,and highlights the possible cellular and molecular mechanism of anti-nutritional effects of SBA.This review has important implications for the prevention and treatment of SBA-induced diseases,drug development,processing techniques of plant products,prevention of food-borne toxins,as well as human and animal health protection.
文摘A novel and efficient method for purification of soybean agglutinin (SBA) from soybean was reported. The method was characterized by selective extraction of SBA from soybean homogenate with barbiturate buffer (pH 6 2), removal of impurity by hydroxyapatite, and the final purification of SBA by guaran affinity chromatography. The purified SBA showed a single band of 27 5 kD by SDS PAGE. The lowest concentration of SBA that caused agglutination of the rabbit red blood cells was 0 31 mg/L. Agglutination of different cancer cell lines by the purified SBA was examined. Strong agglutination of the human nasopharyngeal CNE cells, mouse Lewis lung carcinoma cells, and rat mammary adenocarcinoma R3230AC cells was observed. However, SBA could not agglutinate the human hepatocellular carcinoma BEL 7402 cells, suggesting that unlike the above mentioned three cell lines, the BEL 7402 cells may not express N acetylgalactosamine (GalNAc) or galactose (Gal) residues in significant amount at the non reducing terminals of their cell surface glycans.
