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红球藻水生748株(Haematococcus sp.HB748)培养基的选择与对维生素B_(12)的需求 被引量:17
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作者 金传荫 宋立荣 +4 位作者 刘永定 胡章立 俞家禄 甘小妮 俞敏娟 《应用与环境生物学报》 CAS CSCD 1997年第2期177-179,共3页
比较了红球藻HB748株在MCM,BBM及BG-11.3种培养基中的生长.结果表明:HB748在这3种培养基中前4d的平均生长速率分别为0.97d-1,0.77d-1和0.63d-1,存在显著差异;然而,在BBM和BG-11中添加MCM中所含等量VB12后,748株在3种... 比较了红球藻HB748株在MCM,BBM及BG-11.3种培养基中的生长.结果表明:HB748在这3种培养基中前4d的平均生长速率分别为0.97d-1,0.77d-1和0.63d-1,存在显著差异;然而,在BBM和BG-11中添加MCM中所含等量VB12后,748株在3种培养基中的生长速率趋于一致,表明VB12,是HB748维持较好的前期生长的必需成分;在VB12的需求满足后.3种培养基无机组分的差异对HB748前期生长影响甚微. 展开更多
关键词 藻类养殖 红球藻 培养基 生长 维生素B12
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Biodegradation of phenol by free and immobilized Acinetobacter sp.strain PD12 被引量:24
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作者 WANG Ying TIAN Ye +3 位作者 HAN Bin ZHAO Hua-bing BI Jian-nan CAI Bao-li 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2007年第2期222-225,共4页
A new phenol-degrading bacterium with high biodegradation activity and high tolerance of phenol, strain PD 12, was isolated from the activated sludge of Tianjin Jizhuangzi Wastewater Treatment Facility in China. This ... A new phenol-degrading bacterium with high biodegradation activity and high tolerance of phenol, strain PD 12, was isolated from the activated sludge of Tianjin Jizhuangzi Wastewater Treatment Facility in China. This strain was capable of removing 500 mg phenol/L in liquid minimal medium by 99.6% within 9 h and metabolizing phenol at concentrations up to 1100 mg/L. DNA sequencing and homologous analysis of 16S rRNA gene identified PD12 to be an Acinetobacter sp. Polyvinyl alcohol (PVA) was used as a gel matrix to immobilize Acinetobacter sp. strain PDI2 by repeated freezing and thawing. The factors affecting phenol degradation of immobilized cells were investigated, and the results showed that the immobilized cells could tolerate a high phenol level and protected the bacteria against changes in temperature and pH. Storage stability and reusability tests revealed that the phenol degradation functions of immobilized cells were stable after reuse for 50 times or storing at 4℃ for 50 d. These results indicate that immobilized Acinetobacter sp. strain PD 12 possesses a good application potential in the treatment of phenol-containing wastewater. 展开更多
关键词 PHENOL BIODEGRADATION Acinetobacter sp. PD 12 immobilized bacterium
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Pseudomonas sp.F-12发酵优化及转化合成半胱氨酸的研究
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作者 赵婧楠 李志敏 叶勤 《生物技术通报》 CAS CSCD 北大核心 2014年第10期207-214,共8页
通过不同发酵策略对Pseudomonas sp.F-12以DL-2-氨基-△2-噻唑啉-4-羧酸(DL-ATC)为底物生产L-半胱氨酸的过程进行优化,以分批发酵和指数流加发酵的形式进行生产,将发酵过程中的DL-ATC浓度分别控制在5 g/L和15 g/L。结果表明15 g/L DL-AT... 通过不同发酵策略对Pseudomonas sp.F-12以DL-2-氨基-△2-噻唑啉-4-羧酸(DL-ATC)为底物生产L-半胱氨酸的过程进行优化,以分批发酵和指数流加发酵的形式进行生产,将发酵过程中的DL-ATC浓度分别控制在5 g/L和15 g/L。结果表明15 g/L DL-ATC分批发酵生产L-半胱氨酸得到最高酶活为149.1 U/mL,比活为70.9 U/mg DCW;采用15 g/L DL-ATC两阶段指数流加发酵,可使最高酶活达到313.3 U/mL,提高为分批发酵的2.1倍。同时在两阶段发酵过程中,能减少DL-ATC用量为5 g/L。两阶段发酵过程采用不同流加策略,证明菌体得率对酶活水平有显著影响。首次考察了Pseudomonas sp.F-12全静息细胞转化合成L-半胱氨酸的主要影响因素。结果表明Pseudomonas sp.F-12的最适转化pH为8.0,最适转化温度为30℃。加入甲苯等有机溶剂改变细胞膜通透性,使得半胱氨酸产量比对照组提高7.16倍,最高摩尔转化率达到93.3%。不同金属离子对转化影响不同,0.1 mmol/L Fe2+对转化反应有促进作用,0.1 mmol/L Co2+、Zn2+、Ni2+等重金属离子有抑制作用。研究结果为微生物法发酵及全细胞转化合成半胱氨酸的后续工业化奠定了基础。 展开更多
关键词 假单胞菌 L-半胱氨酸 DL-2-氨基-△2-噻唑啉-4-羧酸(DL-ATC) 发酵 生物转化
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苦竹内生真菌Phomopsis sp.KY-12化学成份及其海虾致死活性研究 被引量:4
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作者 郭芳 杨胜祥 +1 位作者 刘力 王勇 《天然产物研究与开发》 CAS CSCD 北大核心 2014年第9期1389-1392,1439,共5页
利用柱色谱技术从苦竹内生真菌中分离得到13个化合物,包括九个杜松烷型倍半萜、一个azaphilone类化合物和三个甾体类化合物,经波谱鉴定为为3,12-dihydroxycalamenene(1),3,9,12-trihyclroxycalamenene(2),indicumolide C(3... 利用柱色谱技术从苦竹内生真菌中分离得到13个化合物,包括九个杜松烷型倍半萜、一个azaphilone类化合物和三个甾体类化合物,经波谱鉴定为为3,12-dihydroxycalamenene(1),3,9,12-trihyclroxycalamenene(2),indicumolide C(3),agripilol c(4),2,15-tihydroxycalamenene(5),dysodensiol D(6),bombamalones D(7),8-formyl-7-hydroxyl-5-isopropyl-2-methoxy-1,4-naphthoquinone(8),strobilolA(9),PyrenocineJ(10),3JB,5a-Di-hydroxy-6β-methoxyergosta-7,22.dien(11),麦角甾醇(12)和麦角甾醇过氧化物(13)。其中,化合物3-9为首次从该属真菌中分离得到。化合物1—8显示中等程度的细胞毒活性,化合物9显示显著的细胞毒活性。 展开更多
关键词 内生真菌 杜松烷型倍半萜 苦竹 细胞毒活性 PHOMOPSIS sp.
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A new species of the genus Theloderma Tschudi, 1838 (Amphibia: Anura: Rhacophoridae) from Tay Nguyen Plateau, central Vietnam 被引量:1
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作者 Nikolay A. Poyarkov Ivan I. Kropachev +1 位作者 Svetlana S. Gogoleva Nikolai L. Orlov 《Zoological Research》 SCIE CAS CSCD 2018年第3期158-184,共27页
A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloder... A new species of small tree frog from a primary montane tropical forest of central Vietnam, Tay Nguyen Plateau, is described based on morphological, molecular, and acoustic evidence. The Golden Bug-Eyed Frog, Theloderma auratum sp. nov., is distinguishable from its congeners and other small rhacophorid species based on a combination of the following morphological attributes: (1) bony ridges on head absent; (2) smooth skin completely lacking calcified warts or asperities; (3) pointed elongated tapering snout; (4) vocal opening in males absent; (5) vomerine teeth absent; (6) males of small body size (SVL 21.8-26.4 mm); (7) head longer than wide; ED/SVL ratio 13%-15%; ESL/SVL ratio 16%-20%; (8) small tympanum (TD/EL ratio 50%-60%) with few tiny tubercles; (9) supratympanic fold absent; (10) ventral surfaces completely smooth; (11 ) webbing between fingers absent; (12) outer and inner metacarpal tubercles present, supernumerary metacarpal tubercle single, medial, oval in shape; (13) toes half-webbed: I 2-21/4 II 11/^-23/~ III 2-31/4 IV 3-11/2 V; (14) inner metatarsal tubercle present, oval; outer metatarsal tubercle absent; (15) iris bicolored; (16) dorsal surfaces golden-yellow with sparse golden-orange speckling or reticulations and few small dark-brown spots; (17) lateral sides of head and body with wide dark reddish-brown to black lateral stripes, clearlyseparated from lighter dorsal coloration by straight contrasting edge; (18) ventral surfaces of body, throat, and chest greyish-blue with indistinct brown confluent blotches; (19) upper eyelids with few (3-5) very small flat reddish superciliary tubercles; (20) limbs dorsally reddish-brown, ventrally brown with small bluish-white speckles. The new species is also distinct from all congeners in 12S rRNA to 16S rRNA mitochondrial DNA fragment sequences (uncorrected genetic distance P〉8.9%). Advertisement call and tadpole morphology of the new species are described. Our molecular data showed Theloderma auratum sp. nov. to be a sister species of Th. palliatum from Langbian Plateau in southern Vietnam. 展开更多
关键词 Theloderma auratum sp. nov. mtDNAphylogeny 12S rRNA 16S rRNA Kon Tum Gia Lai ENDEMISM Taxonomy
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环糊精糖基转移酶(CGTase)的化学修饰 被引量:5
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作者 曹新志 金征宇 《食品科学》 EI CAS CSCD 北大核心 2004年第12期64-68,共5页
不同蛋白质修饰剂对环糊精葡萄糖基转移酶进行修饰。在一定条件下,分别用丁二酮(DIC)、苯甲酰磺酰氟(PMSF)、二硫苏糖醇(DTT)和氯氨-T(Ch-T)处理后,酶活不受影响,说明精氨酸残基、羟基、二硫键和蛋氨酸残基与酶的活力无关。用焦碳酸二乙... 不同蛋白质修饰剂对环糊精葡萄糖基转移酶进行修饰。在一定条件下,分别用丁二酮(DIC)、苯甲酰磺酰氟(PMSF)、二硫苏糖醇(DTT)和氯氨-T(Ch-T)处理后,酶活不受影响,说明精氨酸残基、羟基、二硫键和蛋氨酸残基与酶的活力无关。用焦碳酸二乙酯(DEPC)、N-溴代琥珀酰亚胺(NBS)和碳化二亚胺(EDC)修饰后,酶活力大幅度下降,说明组氨酸、色氨酸和羧基氨基酸为酶活力所必需。 展开更多
关键词 嗜碱芽孢杆菌 环糊精葡萄糖基转移酶 化学修饰
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FOUNDATION OF NUCLEAR ALGEBRAIC MODELS
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作者 周孝谦 《苏州大学学报(自然科学版)》 CAS 1990年第2期167-176,共10页
Based upon Tomonoga-Rowe's many body theory, we find that the algebraic models, including IBM and FDSM are simplest extension of Rowe-Rosensteel's sp(3R).Dynkin-Gruber's subalgebra embedding method is appl... Based upon Tomonoga-Rowe's many body theory, we find that the algebraic models, including IBM and FDSM are simplest extension of Rowe-Rosensteel's sp(3R).Dynkin-Gruber's subalgebra embedding method is applied to find an appropriate algebra and it's reduction chains conforming to physical requirement. The separated cases sp(6) and so(8) now appear as two branches stemming from the same root D6-O(12). Transitional ease between sp(6) and so(8) is inherently include. 展开更多
关键词 代数模 子代数 嵌入法 HAMILTON函数
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Microscopic shell-model description of irrotational-flow dynamics in^(102)Pd 被引量:1
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作者 H.G.Ganev 《Chinese Physics C》 SCIE CAS CSCD 2024年第1期82-93,共12页
The structure of the low-lying collective excitations in 102Pd is examined within the recently proposed microscopic shell-model version of the Bohr-Mottelson(BM)collective model.A good description of the excitation en... The structure of the low-lying collective excitations in 102Pd is examined within the recently proposed microscopic shell-model version of the Bohr-Mottelson(BM)collective model.A good description of the excitation energies of the lowest ground,γ,andβbands,as well as the staggering function between the collective states of theγband and some other energy-dependent quantities,is obtained.The low-energy intraband and interband quadrupole dynamics is reasonably well described within the present proton-neutron sympletic based shell-model approach without the use of an effective charge and compared with the predictions of nuclear structure models.The obtained results of the present study shed light on the question of the existence of irrotational-flow type quadrupole dynamics,which lies on the ground of the original BM model of quantized vibrations and surface-wave rotations in atomic nuclei. 展开更多
关键词 shell-model version of the Bohr-Mottelson model irrotational-flow dynamics proton-neutron symplectic model sp(12 R)dynamical algebra
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Shell-model representations of the microscopic version of the Bohr-Mottelson collective model
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作者 H G Ganev 《Communications in Theoretical Physics》 SCIE CAS CSCD 2024年第8期79-90,共12页
The structure of the irreducible collective spaces of the group Sp(12,R),which many-particle nuclear states are classified according to the chain Sp(12,R)⊃U(6)⊃SO(6)⊃SU_(pn)(3)⊗SO(2)⊃SO(3)of the proton-neutron symplec... The structure of the irreducible collective spaces of the group Sp(12,R),which many-particle nuclear states are classified according to the chain Sp(12,R)⊃U(6)⊃SO(6)⊃SU_(pn)(3)⊗SO(2)⊃SO(3)of the proton-neutron symplectic model(PNSM),is considered in detail.This chain of the PNSM was recently shown to correspond to a microscopic shell-model version of the Bohr-Mottelson collective model.The construction of the relevant shell-model representations of the Sp(12,R)group along this chain is considered for three nuclei with varying collective properties and from different mass regions.It is shown that the SU_(pn)(3)basis states of the Sp(12,R)representations belonging to the SO(6)irreps with seniorityυ≥υ0,withυ0 denoting the maximal seniority SO(6)irrep contained in the Sp(12,R)bandhead,are always Pauli allowed,but organized in a different way into different SO(6)shells.This is in contrast to the case of filling the levels of the standard three-dimensional harmonic oscillator and using the plethysm operation.Although the SU_(pn)(3)multiplets withinυ<υ0 are not all Pauli forbidden,it is safe to discard them.The results obtained in the present work are important for the practical application of the microscopic version of the Bohr-Mottelson collective model. 展开更多
关键词 microscopic shell-model version of the Bohr-Mottelson model proton-neutron symplectic model sp(12 R)dynamical algebra shell-model representations
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Proton-neutron symplectic model description of ^(106)Cd
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作者 H.G.Ganev 《Chinese Physics C》 SCIE CAS CSCD 2024年第3期169-179,共11页
In this study,a microscopic shell-model description of the low-lying collective states in the weakly deformed nucleus ^(106)Cd within the recently proposed microscopic version of the Bohr-Mottelson model is provided.A... In this study,a microscopic shell-model description of the low-lying collective states in the weakly deformed nucleus ^(106)Cd within the recently proposed microscopic version of the Bohr-Mottelson model is provided.A good description of the excitation energies of the lowest ground,γ,andβquasibands is obtained without the adjustable kinetic energy term.Furthermore,γdegrees of freedom are shown to play a crucial role in the description of spectroscopy of this nucleus.A modified SU(3)SU(3)preserving high-order interaction is used to produce aγ-unstable type of odd-even staggering,observed experimentally between the states of the quasi-γband.The current approach enables the characterization of observed intraband and interband quadrupole collectivity.The findings of this study propose an alternative interpretation of the fundamental question regarding the nature of low-energy vibrations,as well as the emergence of deformation and collectivity in weakly deformed atomic nuclei. 展开更多
关键词 shell-model version of the Bohr-Mottelson model proton-neutron symplectic model sp(12 R)dynamical algebra quadrupole dynamics
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On the microscopic shell-model version of the Bohr-Mottelson collective model 被引量:2
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作者 H.G.Ganev 《Chinese Physics C》 SCIE CAS CSCD 2023年第10期107-119,共13页
The recently proposed microscopic shell-model version of the Bohr-Mottelson(BM) collective model is considered in more detail in the coordinate representation.The latter possesses a clear and transparent physical mean... The recently proposed microscopic shell-model version of the Bohr-Mottelson(BM) collective model is considered in more detail in the coordinate representation.The latter possesses a clear and transparent physical meaning,which reveals several features of the new version of the collective model missed in the previous formulation.The relationship to the original BM model is considered,along with the relationships between the different limiting submodels of the microscopic version of the BM model,which closely resemble the relationships of the original Wilets-Jean and rotor models.The kinematically correct many-particle wave functions of the microscopic version of the BM model,conserving the experimentally observed integrals of motion,are shown to consist of collective irrotational-flow and intrinsic components-in accordance with the original BM unified model.The general BM Hamiltonian is obtained as a contraction limit of the microscopic many-particle nuclear Hamiltonian,or,alternatively,by restricting the latter to the scalar O(m) irreducible collective space. 展开更多
关键词 shell-model version of the Bohr-Mottelson model coordinate representation sp(12 R)dynamical algebra proton-neutron symplectic model
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Matrix elements in the SU(1,1)⊗SO(6)limit of the proton-neutron symplectic model 被引量:3
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作者 H.G.Ganev 《Chinese Physics C》 SCIE CAS CSCD 2021年第11期101-114,共14页
The matrix elements along the reduction chain Sp(12,R)⊃SU(1,1)⊗SO(6)⊃U(1)⊗SUpn(3)⊗SO(2)⊃SO(3)of the proton-neutron symplectic model(PNSM)are considered.Closed analytical expressions are obtained for the matrix element... The matrix elements along the reduction chain Sp(12,R)⊃SU(1,1)⊗SO(6)⊃U(1)⊗SUpn(3)⊗SO(2)⊃SO(3)of the proton-neutron symplectic model(PNSM)are considered.Closed analytical expressions are obtained for the matrix elements of the basic building blocks of the PNSM and the Sp(12,R)symplectic generators,allowing the computation of matrix elements of other physical operators as well.The computational technique developed in the present study generally provides us with the required algebraic tool for performing realistic symplectic-based shell-model calculations of nuclear collective excitations.Utilizing two simple examples,we illustrate the application of the theory. 展开更多
关键词 matrix elements proton-neutron symplectic model SU(1 1)⊗SO(6)limit sp(12 R)dynamical algebra
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一清胶囊联合维胺酯治疗口腔扁平苔藓的临床研究 被引量:3
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作者 马晓华 阚红军 《现代药物与临床》 CAS 2022年第8期1789-1792,共4页
目的探讨一清胶囊联合维胺酯胶囊治疗口腔扁平苔藓的临床疗效。方法选取2019年12月—2021年9月山东省第二人民医院收治的82例口腔扁平苔藓患者,按照随机数字表法将所有患者分为对照组和治疗组,每组各41例。对照组口服维胺酯胶囊,25 mg/... 目的探讨一清胶囊联合维胺酯胶囊治疗口腔扁平苔藓的临床疗效。方法选取2019年12月—2021年9月山东省第二人民医院收治的82例口腔扁平苔藓患者,按照随机数字表法将所有患者分为对照组和治疗组,每组各41例。对照组口服维胺酯胶囊,25 mg/次,3次/d。治疗组在对照组基础上口服一清胶囊,1 g/次,3次/d。两组患者连续治疗3个月。观察两组的临床疗效,比较两组疼痛程度、病损面积和血清中表面活性蛋白(SP)、白细胞介素-8(IL-8)、白细胞介素-12(IL-12)水平。结果治疗后,治疗组患者的总有效率为90.24%,明显高于对照组的总有效率73.17%,组间比较差异有统计学意义(P<0.05)。治疗后,两组的视觉模拟评分法(VAS)评分、病损面积显著降低(P<0.05);治疗组的VAS评分、病损面积低于对照组,差异有统计学意义(P<0.05)。治疗后,两组的血清SP、IL-8、IL-12水平明显降低(P<0.05),治疗组血清SP、IL-8、IL-12水平明显低于对照组(P<0.05)。结论一清胶囊联合维胺酯胶囊治疗口腔扁平苔藓具有较好的临床疗效,能降低疼痛程度和缩小病损面积,调节血清SP、IL-8、IL-12水平。 展开更多
关键词 一清胶囊 维胺酯胶囊 口腔扁平苔藓 VAS评分 病损面积 表面活性蛋白 白细胞介素-8 白细胞介素-12
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基于空腔填充效应构建伯克霍尔德菌脂肪酶LipA的热稳定性突变体
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作者 刘艳如 李鑫 +2 位作者 董盼盼 黄建忠 舒正玉 《微生物学报》 CAS CSCD 北大核心 2019年第8期1535-1546,共12页
[目的]中温伯克霍尔德菌胞外脂肪酶LipA在工业领域具有重要的应用价值。利用蛋白质工程技术来提高其热稳定性,对开发脂肪酶LipA酶制剂及提高其应用范围及应用效果,具有重要的意义。[方法]利用生物信息学软件Castp、Voronoia和Cave分析L... [目的]中温伯克霍尔德菌胞外脂肪酶LipA在工业领域具有重要的应用价值。利用蛋白质工程技术来提高其热稳定性,对开发脂肪酶LipA酶制剂及提高其应用范围及应用效果,具有重要的意义。[方法]利用生物信息学软件Castp、Voronoia和Cave分析LipA分子中存在的空腔及其组成氨基酸残基;利用FoldX软件构建上述氨基酸残基的突变体电子文库,并基于空腔效应(体积变小)、自由能变化值(降低)和空间结构特点等对前述突变体电子文库进行筛选。从突变体电子文库中选择具有代表性的突变体,通过基因工程技术,引入突变。经诱导表达后,实验验证并筛选出热稳定性的突变体。[结果]构建了一个由58个突变体组成的电子文库;并对其中17个代表性的突变体进行了实验验证;筛选到2个热稳定性有明显提高的突变体LipA-His^15Pro和LipA-Ala^210Val;其叠加突变体LipA-His^15Pro/Ala^210Val的T50^12较野生型LipA提高了8℃,在55℃下的半衰期较野生型脂肪酶LipA提高了23.1倍。[结论]基于空腔填充技术构建热稳定性伯克霍尔德菌胞外脂肪酶LipA突变体,是一种行之有效的策略。 展开更多
关键词 伯克霍尔德菌 脂肪酶LipA 空腔填充效应 T50^12 半衰期
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