One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CS-
A chromosome substitution line, CS-B25, was developed by the substitution of chromosome pair 25 of Gossypium hirsutum TM-1 with the homologous pair of chromosome 25 from G. barbadense, a double haploid Pima 3-79 line....A chromosome substitution line, CS-B25, was developed by the substitution of chromosome pair 25 of Gossypium hirsutum TM-1 with the homologous pair of chromosome 25 from G. barbadense, a double haploid Pima 3-79 line. CS-B25 has improved fiber traits compared to its parent TM-1. To explore the molecule mechanisms underlying improved fiber traits, deep sequencing of total RNA was used to compare gene expression in fibers of CS-B25 and TM-1 at 10 days post anthesis (10-DPA). A total of 1872 differentially expressed genes (DEGs) were detected between the two lines, with 1175 up-regulated and 697 down-regulated in CS-B25. Gene Ontology (GO) enrichment analysis of the expression data by Generally Applicable Gene-set Enrichment (GAGE) and ReviGO indicated that the most prevalent Biological Process GO terms associated with DEGs included DNA-templated transcription, response to oxidative stress, and cellulose biosynthesis. Enriched Molecular Function GO terms included structural constituents of cytoskeleton, peroxidase activity, cellulose synthase (UDP-forming) activity, and transcription regulatory region sequence-specific DNA binding factors. GAGE was also used to find enriched KEGG pathways, and the highly represented pathways were Biosynthesis of Amino Acids, Starch and Sucrose Metabolism, Phenylpropanoid Biosynthesis, Protein Processing in Endoplasmic Reticulum, and Plant Hormone Signal Transduction. Many of the identified DEGs are involved in cytoskeleton and cell wall metabolism. The results of gene expression data have provided new insight into the molecular mechanisms of fiber development during the fiber elongation stage and would offer novel candidate genes that may be utilized in cotton fiber quality improvement.展开更多
从棉花cDNA文库中分离了3个编码富含甘氨酸蛋白(glycine-rich proteins,GRPs)的基因,分别命名为GhGRP1、GhGRP2、GhGRP3.推断的编码蛋白质的氨基酸序列都富含甘氨酸,甘氨酸含量超过40%.而且GhGRP1和GhGRP2蛋白质序列同源性高达99%,仅在...从棉花cDNA文库中分离了3个编码富含甘氨酸蛋白(glycine-rich proteins,GRPs)的基因,分别命名为GhGRP1、GhGRP2、GhGRP3.推断的编码蛋白质的氨基酸序列都富含甘氨酸,甘氨酸含量超过40%.而且GhGRP1和GhGRP2蛋白质序列同源性高达99%,仅在C末端有1个氨基酸残基(Arg/Pro)的差别.这3个蛋白在富含甘氨酸区相互显示较高的同源性,GhGRP1与GhGRP2达到100%,而GhGRP2与GhGRP3达到45·1%,但它们与基因数据库中其它蛋白质的同源性很低.根据结构域的组织特点,将GhGRP1和GhGRP2归为C端富含半胱氨酸结构域(C-cysteine-rich)类GRP,将GhGRP3归为N端有信号肽的GRP.GhGRP1和GhGRP2都含有12个GGX(此处X代表P/W/F)重复,GhGRP3含有22个GGX(此处X代表A/F/V/L/T/P)重复.此外,它们还含有不同数量GX,GGGX等的重复.实时RT-PCR分析表明,GhGRP1在花药中优势表达.GhGRP2在10dpa(day post anthesis)胚珠中表达最强,10dpa纤维和下胚轴次之,而在花药、根和花瓣中表达量相对较低.GhGRP3在花药,根和下胚轴中表达量较高,而在子叶,花瓣、纤维和胚珠中表达较低.上述结果表明,GhPRP基因家族的不同成员可能分别在棉花不同组织细胞的发育过程中发挥作用.展开更多
利用cDNA芯片技术分析E6、Lipid transfer protein (LTP)、Proline-rich protein(PRP)、Expansin、Tubu-lin、Annexin等家族的63个棉花纤维发育相关基因,在陆地棉徐州-142开花后10d纤维组织及其无长绒无短绒突变体开花后10d胚珠中的表...利用cDNA芯片技术分析E6、Lipid transfer protein (LTP)、Proline-rich protein(PRP)、Expansin、Tubu-lin、Annexin等家族的63个棉花纤维发育相关基因,在陆地棉徐州-142开花后10d纤维组织及其无长绒无短绒突变体开花后10d胚珠中的表达差异,发现属于E6、LTP、PRP、Expansin家族的基因在两种组织中存在显著的表达差异,符合前人的报道。其中E6、LTP家族的基因在两种组织中的表达差异最大,个别基因表达差异甚至达到15倍之多。而丁Tubulin家族的基因在两种组织中的表达差异不大,检测的24个丁Tubulin家族基因中,仅2个基因在纤维组织和胚珠中存在显著表达差异。cDNA芯片技术可以高通量鉴定棉花纤维相关基因以及研究棉花发育相关基因的表达谱。展开更多
文摘One of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development.Availability of chromosome arm substitution line CS-
文摘A chromosome substitution line, CS-B25, was developed by the substitution of chromosome pair 25 of Gossypium hirsutum TM-1 with the homologous pair of chromosome 25 from G. barbadense, a double haploid Pima 3-79 line. CS-B25 has improved fiber traits compared to its parent TM-1. To explore the molecule mechanisms underlying improved fiber traits, deep sequencing of total RNA was used to compare gene expression in fibers of CS-B25 and TM-1 at 10 days post anthesis (10-DPA). A total of 1872 differentially expressed genes (DEGs) were detected between the two lines, with 1175 up-regulated and 697 down-regulated in CS-B25. Gene Ontology (GO) enrichment analysis of the expression data by Generally Applicable Gene-set Enrichment (GAGE) and ReviGO indicated that the most prevalent Biological Process GO terms associated with DEGs included DNA-templated transcription, response to oxidative stress, and cellulose biosynthesis. Enriched Molecular Function GO terms included structural constituents of cytoskeleton, peroxidase activity, cellulose synthase (UDP-forming) activity, and transcription regulatory region sequence-specific DNA binding factors. GAGE was also used to find enriched KEGG pathways, and the highly represented pathways were Biosynthesis of Amino Acids, Starch and Sucrose Metabolism, Phenylpropanoid Biosynthesis, Protein Processing in Endoplasmic Reticulum, and Plant Hormone Signal Transduction. Many of the identified DEGs are involved in cytoskeleton and cell wall metabolism. The results of gene expression data have provided new insight into the molecular mechanisms of fiber development during the fiber elongation stage and would offer novel candidate genes that may be utilized in cotton fiber quality improvement.
文摘从棉花cDNA文库中分离了3个编码富含甘氨酸蛋白(glycine-rich proteins,GRPs)的基因,分别命名为GhGRP1、GhGRP2、GhGRP3.推断的编码蛋白质的氨基酸序列都富含甘氨酸,甘氨酸含量超过40%.而且GhGRP1和GhGRP2蛋白质序列同源性高达99%,仅在C末端有1个氨基酸残基(Arg/Pro)的差别.这3个蛋白在富含甘氨酸区相互显示较高的同源性,GhGRP1与GhGRP2达到100%,而GhGRP2与GhGRP3达到45·1%,但它们与基因数据库中其它蛋白质的同源性很低.根据结构域的组织特点,将GhGRP1和GhGRP2归为C端富含半胱氨酸结构域(C-cysteine-rich)类GRP,将GhGRP3归为N端有信号肽的GRP.GhGRP1和GhGRP2都含有12个GGX(此处X代表P/W/F)重复,GhGRP3含有22个GGX(此处X代表A/F/V/L/T/P)重复.此外,它们还含有不同数量GX,GGGX等的重复.实时RT-PCR分析表明,GhGRP1在花药中优势表达.GhGRP2在10dpa(day post anthesis)胚珠中表达最强,10dpa纤维和下胚轴次之,而在花药、根和花瓣中表达量相对较低.GhGRP3在花药,根和下胚轴中表达量较高,而在子叶,花瓣、纤维和胚珠中表达较低.上述结果表明,GhPRP基因家族的不同成员可能分别在棉花不同组织细胞的发育过程中发挥作用.
文摘利用cDNA芯片技术分析E6、Lipid transfer protein (LTP)、Proline-rich protein(PRP)、Expansin、Tubu-lin、Annexin等家族的63个棉花纤维发育相关基因,在陆地棉徐州-142开花后10d纤维组织及其无长绒无短绒突变体开花后10d胚珠中的表达差异,发现属于E6、LTP、PRP、Expansin家族的基因在两种组织中存在显著的表达差异,符合前人的报道。其中E6、LTP家族的基因在两种组织中的表达差异最大,个别基因表达差异甚至达到15倍之多。而丁Tubulin家族的基因在两种组织中的表达差异不大,检测的24个丁Tubulin家族基因中,仅2个基因在纤维组织和胚珠中存在显著表达差异。cDNA芯片技术可以高通量鉴定棉花纤维相关基因以及研究棉花发育相关基因的表达谱。
