[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media ...[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media as MS, B5, Nitsch and Wpm and the ratio of two hormones(1, 2, 3 mg/L 6-BA and 0.1, 0.3, 0.5 mg/L NAA) on bud proliferation of S. kochii were studied by the complete test method, and the effect of the ratio of the two hormones(0.25, 0.50, 1.00 mg/L NAA and 1.0, 1.5 mg/L IBA) on rooting of S. kochii as well as the effect of substrate ratio(perlite and peat soil at 1∶9, 2∶8, 3∶7, 4∶6 and 5∶5) on its transplanting survival rate were also studied. [Results] The best basic medium for the rapid propagation of S. kochii was MS. The best hormone ratio suitable for bud proliferation was 2 mg/L6-BA+0.1 mg/L NAA, and the average number of buds proliferated at 45 d was 3.04. The average bud height was 2.05 cm; the most suitable medium for rooting was 1/2 MS+1 mg/L IBA+0.25 mg/L NAA, and its rooting rate was 100%; and the best transplanting substrate was 5∶5 perlite and peat. The soil ratio had a survival rate of 94%. [Conclusions] This study provides a theoretical basis for the improvement of the transplanting survival rate of test-tube S. kochii plantlets.展开更多
[Objectives]This study was conducted to provide technical standards for domestic Spathiphyllum variety description and resource evaluation.[Methods]Through field trials and character surveys of the collected 83 Spathi...[Objectives]This study was conducted to provide technical standards for domestic Spathiphyllum variety description and resource evaluation.[Methods]Through field trials and character surveys of the collected 83 Spathiphyllum varieties,after several revisions by fellow experts,and with reference to the Guidelines for Testing the Specificity,Consistency and Stability of New Plant Varieties:Spathiphyllum(TG/135/3)issued by the International Union For The Protection of New Varieties of Plants,the DUS testing guidelines for Spathiphyllum have been developed,and statistical analysis and other methods were used to study the traits for the guideline.[Results]Thirty two testing traits,including 11 mandatory traits,8 basic traits,10 new traits,and 3 optional traits were finally determined;five traits including plant height,plant width,and leaf length were divided into 7 grades,and 9 traits such as leaf width,spathe length,and spathe width were divided into 5 levels;and the observation method and trait code of the trait spathe depth were adjusted.[Conclusions]The development of this guideline provides a basis for judging the specificity,consistency and stability of Spathiphyllum varieties,and is of great significance to the breeding and protection of Spathiphyllum varieties.展开更多
基金Supported by Research Project of Jingmen Science and Technology Bureau(YDKY2017008)
文摘[Objectives] This study was conducted on tissue-culture rapid propagation techniques of Spathiphyllum kochii Engl. et Krause. [Methods] With lateral buds of S. kochii as explants, the effects of such four basic media as MS, B5, Nitsch and Wpm and the ratio of two hormones(1, 2, 3 mg/L 6-BA and 0.1, 0.3, 0.5 mg/L NAA) on bud proliferation of S. kochii were studied by the complete test method, and the effect of the ratio of the two hormones(0.25, 0.50, 1.00 mg/L NAA and 1.0, 1.5 mg/L IBA) on rooting of S. kochii as well as the effect of substrate ratio(perlite and peat soil at 1∶9, 2∶8, 3∶7, 4∶6 and 5∶5) on its transplanting survival rate were also studied. [Results] The best basic medium for the rapid propagation of S. kochii was MS. The best hormone ratio suitable for bud proliferation was 2 mg/L6-BA+0.1 mg/L NAA, and the average number of buds proliferated at 45 d was 3.04. The average bud height was 2.05 cm; the most suitable medium for rooting was 1/2 MS+1 mg/L IBA+0.25 mg/L NAA, and its rooting rate was 100%; and the best transplanting substrate was 5∶5 perlite and peat. The soil ratio had a survival rate of 94%. [Conclusions] This study provides a theoretical basis for the improvement of the transplanting survival rate of test-tube S. kochii plantlets.
文摘[Objectives]This study was conducted to provide technical standards for domestic Spathiphyllum variety description and resource evaluation.[Methods]Through field trials and character surveys of the collected 83 Spathiphyllum varieties,after several revisions by fellow experts,and with reference to the Guidelines for Testing the Specificity,Consistency and Stability of New Plant Varieties:Spathiphyllum(TG/135/3)issued by the International Union For The Protection of New Varieties of Plants,the DUS testing guidelines for Spathiphyllum have been developed,and statistical analysis and other methods were used to study the traits for the guideline.[Results]Thirty two testing traits,including 11 mandatory traits,8 basic traits,10 new traits,and 3 optional traits were finally determined;five traits including plant height,plant width,and leaf length were divided into 7 grades,and 9 traits such as leaf width,spathe length,and spathe width were divided into 5 levels;and the observation method and trait code of the trait spathe depth were adjusted.[Conclusions]The development of this guideline provides a basis for judging the specificity,consistency and stability of Spathiphyllum varieties,and is of great significance to the breeding and protection of Spathiphyllum varieties.
