Traditional Chinese medicine(TCM)is a treasure of the Chinese nation,providing effective solutions to current medical requisites.Various spectral techniques are undergoing continuous development and provide new and re...Traditional Chinese medicine(TCM)is a treasure of the Chinese nation,providing effective solutions to current medical requisites.Various spectral techniques are undergoing continuous development and provide new and reliable means for evaluating the efficacy and quality of TCM.Because spectral techniques are noninvasive,convenient,and sensitive,they have been widely applied to in vitro and in vivo TCM evaluation systems.In this paper,previous achievements and current progress in the research on spectral technologies(including fluorescence spectroscopy,photoacoustic imaging,infrared thermal imaging,laser-induced breakdown spectroscopy,hyperspectral imaging,and surface enhanced Raman spectroscopy)are discussed.The advantages and disadvantages of each technology are also presented.Moreover,the future applications of spectral imaging to identify the origins,components,and pesticide residues of TCM in vitro are elucidated.Subsequently,the evaluation of the efficacy of TCM in vivo is presented.Identifying future applications of spectral imaging is anticipated to promote medical research as well as scientific and technological explorations.展开更多
Multi-channel polarization optical technology is increasingly used for prompt monitoring of water systems.Optical devices during the assessment of water quality determine the intensity of light through the studied aqu...Multi-channel polarization optical technology is increasingly used for prompt monitoring of water systems.Optical devices during the assessment of water quality determine the intensity of light through the studied aquatic environment.Spectrophotometric devices measure the spectrum of weakening of light through the aquatic environment.Spectroellipsometric devices receive spectra in vertical and horizontal polarizations.The presented article develops an adaptive optical hardware and image system for monitoring water bodies.The system is combined.It consists of 2 parts:1)automated spectrophotometer-refractometer,and 2)adaptive spectroellipsometer.The system is equipped with a corresponding algorithmic and software,including algorithms for identifying spectral curves,databases and knowledge of spectral curves algorithms for solving reverse problems.The presented system is original since it differs from modern foreign systems by a new method of spectrophotometric and spectroellipsometric measurements,an original elemental base of polarization optics and a comprehensive mathematical approach to assessing the quality of a water body.There are no rotating polarization elements in the system.Therefore,this makes it possible to increase the signal-to-noise ratio and,as a result,improve measurement stability and simplify multichannel spectrophotometers and spectroellipsometers.The proposed system can be used in various water systems where it is necessary to assess water quality or identify the presence of a certain set of chemical elements.展开更多
Fresnel incoherent correlation holography(FINCH) is a unique three-dimensional(3D) imaging technique which has the advantages of scanning-free,high resolution,and easy matching with existing mature optical systems.In ...Fresnel incoherent correlation holography(FINCH) is a unique three-dimensional(3D) imaging technique which has the advantages of scanning-free,high resolution,and easy matching with existing mature optical systems.In this article,an incoherent digital holographic spectral imaging method with high accuracy of spectral reconstruction based on liquid crystal tunable filter(LCTF) and FINCH is proposed.Using the programmable characteristics of spatial light modulator(SLM),a series of phase masks,none of whose focal lengths changes with wavelength,is designed and made.For each wavelength of LCTF output,SLM calls three phase masks with different phase constants at the corresponding wavelength,and CCD records three holograms.The spectral images obtained by this method have a constant magnification,which can achieve pixel-level image registration,restrain image registration errors,and improve spectral reconstruction accuracy.The results show that this method can not only obtain the 3D spatial information and spectral information of the object simultaneously,but also have high accuracy of spectral reconstruction and excellent color reproducibility.展开更多
Authentication of pasta is currently determined using molecular biology-based techniques focusing on DNA as the target analyte. Whilst proven to be effective, these approaches can be criticised as being destructive, t...Authentication of pasta is currently determined using molecular biology-based techniques focusing on DNA as the target analyte. Whilst proven to be effective, these approaches can be criticised as being destructive, time consuming, and requiring specialist instrument training. Advances in the field of multispectral imaging (MSI) and hyperspectral imaging (HSI) have facilitated the development of compact imaging platforms with the capability to rapidly differentiate a range of materials (inclusive of grains and seeds) based on surface colour, texture and chemical composition. This preliminary investigation evaluated the applicability of spectral imaging for identification and quantitation of durum wheat grain samples in relation to pasta authenticity. MSI and HSI were capable of rapidly distinguishing between durum wheat and adulterant common wheat cultivars and assigning percentage adulteration levels characterised by low biases and good repeatability estimates. The results demonstrated the potential for spectral imaging based seed/grain adulteration testing to augment existing standard molecular approaches for food authenticity testing.展开更多
The problem of in vrivo photoluminescence diagnostics of the tissues acessible by endoscopes is discussed.The spectral imaging module attachable to conventional rigid and flexible medical endoscopes is developed and d...The problem of in vrivo photoluminescence diagnostics of the tissues acessible by endoscopes is discussed.The spectral imaging module attachable to conventional rigid and flexible medical endoscopes is developed and described.It is based on a double acousto-optical tunable filter(AOTF)and a specialized optical coupling system.The module provides wide field of view(FOV),absence of image distortions,random spectral access,fast spectral image acquisition at any wavelength in the visible range and accurate measurement of reflectance spectrum in each pixel of the image.Images of typical biomedical samples are presented and discussed.Their spectra are compared to the reference data.展开更多
The application to detect ilally added drugs in dietary supplerments by near-infrared spectral imaging was studied with the focus on nifedipine,diclofenac and metformin.The method is based on near-infrared spectral im...The application to detect ilally added drugs in dietary supplerments by near-infrared spectral imaging was studied with the focus on nifedipine,diclofenac and metformin.The method is based on near-infrared spectral images correlation cofficient to detect ilally added drugs.The results comply 100%with HPLC methods test results with no false positive results.展开更多
An introduction to the basics of spectral imaging as applied to biological tissues is presented.An example of a spectral image of a face is used to demonstrate the data and spectral analysis that specify the melanin c...An introduction to the basics of spectral imaging as applied to biological tissues is presented.An example of a spectral image of a face is used to demonstrate the data and spectral analysis that specify the melanin content(M),blood content(B),tissue oxygen saturation(S),water content(W),fraction of scattering due to Rayleigh scattering(f)and due to Mie scattering(1−f),and the reduced scattering coefficient at 500-nm wavelength(µs 500 nm).The sensitivity of reflectance spectra to variation in the various parameters is illustrated.展开更多
Spectral imaging is an important tool for a wide variety of applications. We present a technique for spectral imaging using computational imaging pattern based on compressive sensing (CS). The spectral and spatial i...Spectral imaging is an important tool for a wide variety of applications. We present a technique for spectral imaging using computational imaging pattern based on compressive sensing (CS). The spectral and spatial infor- mation is simultaneously obtained using a fiber spectrometer and the spatial light modulation without mechanical scanning. The method allows high-speed, stable, and sub sampling acquisition of spectral data from specimens. The relationship between sampling rate and image quality is discussed and two CS algorithms are compared.展开更多
Oil spills pose a major threat to ocean ecosystems and their health. Synthetic aperture radar(SAR) sensors can detect oil spills on the sea surface. These oil spills appear as dark spots in SAR images. However, dark...Oil spills pose a major threat to ocean ecosystems and their health. Synthetic aperture radar(SAR) sensors can detect oil spills on the sea surface. These oil spills appear as dark spots in SAR images. However, dark formations can be caused by a number of phenomena. It is aimed to distinguishing oil spills or look-alike objects. A novel method based on a bidimensional empirical mode decomposition is proposed. The selected dark formations are first decomposed into several bidimensional intrinsic mode functions and the residue. Subsequently, 64 dimension feature sets are calculated using the Hilbert spectral analysis and five new features are extracted with a relief algorithm. Mahalanobis distances are then used for classification. Three data sets containing oil spills or look-alikes are used to test the accuracy rate of the method. The accuracy rate is more than 90%. The experimental results demonstrate that the novel method can detect oil spills validly and accurately.展开更多
We have examined ten human subjects with a previously developed instrument for near-infrared diffuse spectral imaging of the female breast.The instrument is based on a tandem,planar scan of two collinear optical fiber...We have examined ten human subjects with a previously developed instrument for near-infrared diffuse spectral imaging of the female breast.The instrument is based on a tandem,planar scan of two collinear optical fibers(one for illumination and one for collection)to image a gently compressed breast in a transmission geometry.The optical data collection features a spatial sampling of 25 points/cm2 over the whole breast,and a spectral sampling of 2 points/nm in the 650-900nm wavelength range.Of the ten human subjects examined,eight are healthy subjects and two are cancer patients with unilateral invasive ductal carcinoma and ductal carcinoma in situ,respectively.For each subject,we generate second-derivative images that identify a network of highly absorbing structures in the breast that we assign to blood vessels.A previously developed paired-wavelength spectral method assigns oxygenation values to the absorbing structures displayed in the second-derivative images.The resulting oxygenation images feature average values over the whole breast that are significantly lower in cancerous breasts(69±14%,n=2)than in healthy breasts(85±7%,n=18)(p<0.01).Furthermore,in the two patients with breast cancer,the average oxygenation values in the cancerous regions are also significantly lower than in the remainder of the breast(invasive ductal carcinoma:49±11%vs 61±16%,p<0.01;ductal carcinoma in situ:58±8%vs 77±11%,p<0.001).展开更多
The content of berberine hydrochloride(BH)in compound berberine tablets(CBTs)is subject to strict requirements.Its content is usually measured based on chemical analysis.In this paper,the fluorescence spectral imaging...The content of berberine hydrochloride(BH)in compound berberine tablets(CBTs)is subject to strict requirements.Its content is usually measured based on chemical analysis.In this paper,the fluorescence spectral imaging method was used to study the relative content of BH from a physics perspective.By comparing the relative fluorescence intensity of self-made CBTs with di®erent mass percentages of BH,a linear positive relationship was observed between the BH content and the relative fluorescence intensity,and accordingly the quality of CBTs of different brands was evaluated.The results indicate that the fluorescence spectral imaging method can be a simple,fast and nondestructive semi-quantitative analysis method to determine the content of BH in CBTs,and this method has great potential in the quality control of CBTs.展开更多
Coded aperture snapshot spectral imaging(CASSI) has been discussed in recent years. It has the remarkable advantages of high optical throughput, snapshot imaging, etc. The entire spatial-spectral data-cube can be reco...Coded aperture snapshot spectral imaging(CASSI) has been discussed in recent years. It has the remarkable advantages of high optical throughput, snapshot imaging, etc. The entire spatial-spectral data-cube can be reconstructed with just a single two-dimensional(2D) compressive sensing measurement. On the other hand, for less spectrally sparse scenes,the insufficiency of sparse sampling and aliasing in spatial-spectral images reduce the accuracy of reconstructed threedimensional(3D) spectral cube. To solve this problem, this paper extends the improved CASSI. A band-pass filter array is mounted on the coded mask, and then the first image plane is divided into some continuous spectral sub-band areas. The entire 3D spectral cube could be captured by the relative movement between the object and the instrument. The principle analysis and imaging simulation are presented. Compared with peak signal-to-noise ratio(PSNR) and the information entropy of the reconstructed images at different numbers of spectral sub-band areas, the reconstructed 3D spectral cube reveals an observable improvement in the reconstruction fidelity, with an increase in the number of the sub-bands and a simultaneous decrease in the number of spectral channels of each sub-band.展开更多
Skin scar is unique to humans,the major significant negative outcome sustained after thermal injuries,traumatic injuries,and surgical procedures.Hypertrophic scar in human skin is investigated using non-linear spectra...Skin scar is unique to humans,the major significant negative outcome sustained after thermal injuries,traumatic injuries,and surgical procedures.Hypertrophic scar in human skin is investigated using non-linear spectral imaging microscopy.The high contrast images and spectroscopic intensities of collagen and elastic fibers extracted from the spectral imaging of normal skin tissue,and the normal skin near and far away from the hypertrophic scar tissues in a 10-year-old patient case are obtained.The results show that there are apparent differences in the morphological structure and spectral characteristics of collagen and elastic fibers when comparing the normal skin with the hypertrophic scar tissue.These differences can be good indicators to differentiate the normal skin and hypertrophic scar tissue and demonstrate that non-linear spectral imaging microscopy has potential to noninvasively investigate the pathophysiology of human hypertrophic scar.展开更多
AIM: To determine angle closure agreements between gonioscopy and anterior segment optical coherence tomography(AS-OCT), as well as gonioscopy and spectral domain OCT(SD-OCT). A secondary objective was to quantify int...AIM: To determine angle closure agreements between gonioscopy and anterior segment optical coherence tomography(AS-OCT), as well as gonioscopy and spectral domain OCT(SD-OCT). A secondary objective was to quantify inter-observer agreements of AS-OCT and SD-OCT assessments.METHODS: Seventeen consecutive subjects(33 eyes)were recruited from the study hospital’s Glaucoma clinic.Gonioscopy was performed by a glaucomatologist masked to OCT results. OCT images were read independently by 2 other glaucomatologists masked to gonioscopy findings as well as each other’s analyses of OCT images.RESULTS: Totally 84.8% and 45.5% of scleral spurs were visualized in AS-OCT and SD-OCT images respectively(P 【0.01). The agreement for angle closure between AS-OCT and gonioscopy was fair at k =0.31(95% confidence interval, CI: 0.03-0.59) and k =0.35(95%CI: 0.07-0.63) for reader 1 and 2 respectively. The agreement for angle closure between SD-OCT and gonioscopy was fair at k =0.21(95% CI: 0.07-0.49) and slight at k =0.17(95% CI: 0.08-0.42) for reader 1 and 2 respectively. The inter-reader agreement for angle closure in AS-OCT images was moderate at 0.51(95% CI: 0.13-0.88). The inter-reader agreement for angle closure in SD-OCT images was slight at 0.18(95% CI: 0.08-0.45).CONCLUSION: Significant proportion of scleral spurs were not visualised with SD-OCT imaging resulting in weaker inter-reader agreements. Identifying other angle landmarks in SD-OCT images will allow more consistent angle closure assessments. Gonioscopy and OCT imaging do not always agree in angle closure assessments but have their own advantages, and should be used together and not exclusively.展开更多
Neural network analysis based on Growing Hierarchical Self-Organizing Map (GHSOM) is used to examine Spatial-Temporal characteristics in Aerosol Optical Depth (AOD), Ångström Exponent (ÅE)...Neural network analysis based on Growing Hierarchical Self-Organizing Map (GHSOM) is used to examine Spatial-Temporal characteristics in Aerosol Optical Depth (AOD), Ångström Exponent (ÅE) and Precipitation Rate (PR) over selected East African sites from 2000 to 2014. The selected sites of study are Nairobi (1°S, 36°E), Mbita (0°S, 34°E), Mau Forest (0.0° - 0.6°S;35.1°E - 35.7°E), Malindi (2°S, 40°E), Mount Kilimanjaro (3°S, 37°E) and Kampala (0°N, 32.1°E). GHSOM analysis reveals a marked spatial variability in AOD and ÅE that is associated to changing PR, urban heat islands, diffusion, direct emission, hygroscopic growth and their scavenging from the atmosphere specific to each site. Furthermore, spatial variability in AOD, ÅE and PR is distinct since each variable corresponds to a unique level of classification. On the other hand, GHSOM algorithm efficiently discriminated by means of clustering between AOD, ÅE and PR during Long and Short rain spells and dry spell over each variable emphasizing their temporal evolution. The utilization of GHSOM therefore confirms the fact that regional aerosol characteristics are highly variable be it spatially or temporally and as well modulated by PR received over each variable.展开更多
Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)mic...Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)microscopy is a rapidly growing technique that has attracted broad attentions and become a powerful tool for biology and biomedicine,largely thanks to its chemical specicity,high sensitivity and fast image speed.This review paper introduces the principles of SRS,discusses the technical developments and implementations of SRS microscopy,then highlights and summarizes its applications on biological cellular machinery andnally shares our visions of potential breakthroughs in the future.展开更多
With the rapid development of location-based services and online social networks,POI recommendation services considering geographic and social factors have received extensive attention.Meanwhile,the vigorous developme...With the rapid development of location-based services and online social networks,POI recommendation services considering geographic and social factors have received extensive attention.Meanwhile,the vigorous development of cloud computing has prompted service providers to outsource data to the cloud to provide POI recommendation services.However,there is a degree of distrust of the cloud by service providers.To protect digital assets,service providers encrypt data before outsourcing it.However,encryption reduces data availability,making it more challenging to provide POI recommendation services in outsourcing scenarios.Some privacy-preserving schemes for geo-social-based POI recommendation have been presented,but they have some limitations in supporting group query,considering both geographic and social factors,and query accuracy,making these schemes impractical.To solve this issue,we propose two practical and privacy-preserving geo-social-based POI recommendation schemes for single user and group users,which are named GSPR-S and GSPR-G.Specifically,we first utilize the quad tree to organize geographic data and the MinHash method to index social data.Then,we apply BGV fully homomorphic encryption to design some private algorithms,including a private max/min operation algorithm,a private rectangular set operation algorithm,and a private rectangular overlapping detection algorithm.After that,we use these algorithms as building blocks in our schemes for efficiency improvement.According to security analysis,our schemes are proven to be secure against the honest-but-curious cloud servers,and experimental results show that our schemes have good performance.展开更多
Sustainable forest management is essential to confront the detrimental impacts of diseases on forest ecosystems.This review highlights the potential of vegetation spectroscopy in improving the feasibility of assessing...Sustainable forest management is essential to confront the detrimental impacts of diseases on forest ecosystems.This review highlights the potential of vegetation spectroscopy in improving the feasibility of assessing forest disturbances induced by diseases in a timely and cost-effective manner.The basic concepts of vegetation spectroscopy and its application in phytopathology are first outlined then the literature on the topic is discussed.Using several optical sensors from leaf to landscape-level,a number of forest diseases characterized by variable pathogenic processes have been detected,identified and quantified in many country sites worldwide.Overall,these reviewed studies have pointed out the green and red regions of the visible spectrum,the red-edge and the early near-infrared as the spectral regions most sensitive to the disease development as they are mostly related to chlorophyll changes and symptom development.Late disease conditions particularly affect the shortwave-infrared region,mostly related to water content.This review also highlights some major issues to be addressed such as the need to explore other major forest diseases and geographic areas,to further develop hyperspectral sensors for early detection and discrimination of forest disturbances,to improve devices for remote sensing,to implement longterm monitoring,and to advance algorithms for exploitation of spectral data.Achieving of these goals will enhance the capability of vegetation spectroscopy in early detection of forest stress and in managing forest diseases.展开更多
Spectral imaging extends the concept of traditional color cameras to capture images across multiple spectral channels and has broad ap-plication prospects.Conventional spectral cameras based on scanning methods suffer...Spectral imaging extends the concept of traditional color cameras to capture images across multiple spectral channels and has broad ap-plication prospects.Conventional spectral cameras based on scanning methods suffer from the drawbacks of low acquisition speed and large volume.On-chip computational spectral imaging based on metasur-face filters provides a promising scheme for portable applications,but endures long computation time due to point-by-point iterative spec-tral reconstruction and mosaic effect in the reconstructed spectral im-ages.In this study,on-chip rapid spectral imaging was demonstrated,which eliminated the mosaic effect in the spectral image by deep-learning-based spectral data cube reconstruction.The experimental results show that 4 orders of magnitude faster than the iterative spec-tral reconstruction were achieved,and the fidelity of the spectral re-construction for the standard color plate was over 99%for a standard color board.In particular,video-rate spectral imaging was demon-strated for moving objects and outdoor driving scenes with good per-formance for recognizing metamerism,where the concolorous sky and white cars can be distinguished via their spectra,showing great po-tential for autonomous driving and other practical applications in the field of intelligent perception.展开更多
AIM: To evaluate whether virtual chromoendoscopy can improve the delineation of small bowel lesions previously detected by conventional white light small bowel capsule endoscopy(SBCE). METHODS: Retrospective single ce...AIM: To evaluate whether virtual chromoendoscopy can improve the delineation of small bowel lesions previously detected by conventional white light small bowel capsule endoscopy(SBCE). METHODS: Retrospective single center study. One hundred lesions selected from forty-nine consecutive conventional white light SBCE(SBCE-WL) examinations were included. Lesions were reviewed at three Flexible Spectral Imaging Color Enhancement(FICE) settings and Blue Filter(BF) by two gastroenterologists with ex-perience in SBCE, blinded to each other's findings, whoranked the quality of delineation as better, equivalent or worse than conventional SBCE-WL. Inter-observer percentage of agreement was determined and analyzed with Fleiss Kappa(k) coefficient. Lesions selected for the study included angioectasias(n = 39), ulcers/ero-sions(n = 49) and villous edema/atrophy(n = 12). RESULTS: Overall, the delineation of lesions was im-proved in 77% of cases with FICE 1, 74% with FICE 2, 41% with FICE 3 and 39% with the BF, with a percent-age of agreement between investigators of 89%(k = 0.833), 85%(k = 0.764), 66%(k = 0.486) and 79%(k = 0.593), respectively. FICE 1 improved the delineation of 97.4% of angioectasias, 63.3% of ulcers/erosions and 66.7% of villous edema/atrophy with a percentage of agreement of 97.4%(k = 0.910), 81.6%(k = 0.714) and 91.7%(k = 0.815), respectively. FICE 2 improved the delineation of 97.4% of angioectasias, 57.1% of ulcers/erosions and 66.7% of villous edema/atrophy, with a percentage of agreement of 89.7%(k = 0.802), 79,6%(k = 0.703) and 91.7%(k = 0.815), respectively. FICE 3 improved the delineation of 46.2% of angioecta-sias, 24.5% of ulcers/erosions and none of the cases of villous edema/atrophy, with a percentage of agreement of 53.8% [k = not available(NA)], 75.5%(k = NA) and 66.7%(k = 0.304), respectively. The BF improved the delineation of 15.4% of angioectasias, 61.2% of ulcers/erosions and 25% of villous edema/atrophy, with a per-centage of agreement of 76.9%(k = 0.558), 81.6%(k = 0.570) and 25.0%(k = NA), respectively.CONCLUSION: Virtual chromoendoscopy can improve the delineation of angioectasias, ulcers/erosions and villous edema/atrophy detected by SBCE, with almost perfect interobserver agreement for FICE 1.展开更多
基金supported by the National Key R&D Program of China(Grant No.:2017YFC1702003)the Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(Grant No.:2019e12M-5-078).
文摘Traditional Chinese medicine(TCM)is a treasure of the Chinese nation,providing effective solutions to current medical requisites.Various spectral techniques are undergoing continuous development and provide new and reliable means for evaluating the efficacy and quality of TCM.Because spectral techniques are noninvasive,convenient,and sensitive,they have been widely applied to in vitro and in vivo TCM evaluation systems.In this paper,previous achievements and current progress in the research on spectral technologies(including fluorescence spectroscopy,photoacoustic imaging,infrared thermal imaging,laser-induced breakdown spectroscopy,hyperspectral imaging,and surface enhanced Raman spectroscopy)are discussed.The advantages and disadvantages of each technology are also presented.Moreover,the future applications of spectral imaging to identify the origins,components,and pesticide residues of TCM in vitro are elucidated.Subsequently,the evaluation of the efficacy of TCM in vivo is presented.Identifying future applications of spectral imaging is anticipated to promote medical research as well as scientific and technological explorations.
基金Supported By The Russian Science Foundation Grant No.23-21-00115,https://rscf.ru/en/project/23-21-00115/.
文摘Multi-channel polarization optical technology is increasingly used for prompt monitoring of water systems.Optical devices during the assessment of water quality determine the intensity of light through the studied aquatic environment.Spectrophotometric devices measure the spectrum of weakening of light through the aquatic environment.Spectroellipsometric devices receive spectra in vertical and horizontal polarizations.The presented article develops an adaptive optical hardware and image system for monitoring water bodies.The system is combined.It consists of 2 parts:1)automated spectrophotometer-refractometer,and 2)adaptive spectroellipsometer.The system is equipped with a corresponding algorithmic and software,including algorithms for identifying spectral curves,databases and knowledge of spectral curves algorithms for solving reverse problems.The presented system is original since it differs from modern foreign systems by a new method of spectrophotometric and spectroellipsometric measurements,an original elemental base of polarization optics and a comprehensive mathematical approach to assessing the quality of a water body.There are no rotating polarization elements in the system.Therefore,this makes it possible to increase the signal-to-noise ratio and,as a result,improve measurement stability and simplify multichannel spectrophotometers and spectroellipsometers.The proposed system can be used in various water systems where it is necessary to assess water quality or identify the presence of a certain set of chemical elements.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61505178,61307019,and 11504333)the Natural Science Foundation of Henan Province,China(Grant Nos.18A140032,15A140038,and 16A140035)。
文摘Fresnel incoherent correlation holography(FINCH) is a unique three-dimensional(3D) imaging technique which has the advantages of scanning-free,high resolution,and easy matching with existing mature optical systems.In this article,an incoherent digital holographic spectral imaging method with high accuracy of spectral reconstruction based on liquid crystal tunable filter(LCTF) and FINCH is proposed.Using the programmable characteristics of spatial light modulator(SLM),a series of phase masks,none of whose focal lengths changes with wavelength,is designed and made.For each wavelength of LCTF output,SLM calls three phase masks with different phase constants at the corresponding wavelength,and CCD records three holograms.The spectral images obtained by this method have a constant magnification,which can achieve pixel-level image registration,restrain image registration errors,and improve spectral reconstruction accuracy.The results show that this method can not only obtain the 3D spatial information and spectral information of the object simultaneously,but also have high accuracy of spectral reconstruction and excellent color reproducibility.
文摘Authentication of pasta is currently determined using molecular biology-based techniques focusing on DNA as the target analyte. Whilst proven to be effective, these approaches can be criticised as being destructive, time consuming, and requiring specialist instrument training. Advances in the field of multispectral imaging (MSI) and hyperspectral imaging (HSI) have facilitated the development of compact imaging platforms with the capability to rapidly differentiate a range of materials (inclusive of grains and seeds) based on surface colour, texture and chemical composition. This preliminary investigation evaluated the applicability of spectral imaging for identification and quantitation of durum wheat grain samples in relation to pasta authenticity. MSI and HSI were capable of rapidly distinguishing between durum wheat and adulterant common wheat cultivars and assigning percentage adulteration levels characterised by low biases and good repeatability estimates. The results demonstrated the potential for spectral imaging based seed/grain adulteration testing to augment existing standard molecular approaches for food authenticity testing.
基金supported by Grant Nos.13-02-12210 and 14-00-10420 of Russian Foundation of Basic Research.
文摘The problem of in vrivo photoluminescence diagnostics of the tissues acessible by endoscopes is discussed.The spectral imaging module attachable to conventional rigid and flexible medical endoscopes is developed and described.It is based on a double acousto-optical tunable filter(AOTF)and a specialized optical coupling system.The module provides wide field of view(FOV),absence of image distortions,random spectral access,fast spectral image acquisition at any wavelength in the visible range and accurate measurement of reflectance spectrum in each pixel of the image.Images of typical biomedical samples are presented and discussed.Their spectra are compared to the reference data.
基金support of National Science and Technology Support Program (2012BAK08B02)Beijing Institute for Drug Control and Jiangsu Institute for Food and Drug Control for their generous providing of dietary supplements samples.
文摘The application to detect ilally added drugs in dietary supplerments by near-infrared spectral imaging was studied with the focus on nifedipine,diclofenac and metformin.The method is based on near-infrared spectral images correlation cofficient to detect ilally added drugs.The results comply 100%with HPLC methods test results with no false positive results.
基金the National Institutes of Health(RO1-HL084013).
文摘An introduction to the basics of spectral imaging as applied to biological tissues is presented.An example of a spectral image of a face is used to demonstrate the data and spectral analysis that specify the melanin content(M),blood content(B),tissue oxygen saturation(S),water content(W),fraction of scattering due to Rayleigh scattering(f)and due to Mie scattering(1−f),and the reduced scattering coefficient at 500-nm wavelength(µs 500 nm).The sensitivity of reflectance spectra to variation in the various parameters is illustrated.
基金Supported by the National Major Scientific Instruments Development Project of China under Grant No 2013YQ030595the National Natural Science Foundation of China under Grant Nos 11675014,61601442,61605218,61474123 and 61575207+2 种基金the Science and Technology Innovation Foundation of Chinese Academy of Sciences under Grant No CXJJ-16S047,the National Defense Science and Technology Innovation Foundation of Chinese Academy of Sciencesthe Program of International S&T Cooperation under Grant No 2016YFE0131500the Advance Research Project under Grant No 30102070101
文摘Spectral imaging is an important tool for a wide variety of applications. We present a technique for spectral imaging using computational imaging pattern based on compressive sensing (CS). The spectral and spatial infor- mation is simultaneously obtained using a fiber spectrometer and the spatial light modulation without mechanical scanning. The method allows high-speed, stable, and sub sampling acquisition of spectral data from specimens. The relationship between sampling rate and image quality is discussed and two CS algorithms are compared.
基金The National Science and Technology Support Project under contract No.2014BAB12B02the Natural Science Foundation of Liaoning Province under contract No.201602042
文摘Oil spills pose a major threat to ocean ecosystems and their health. Synthetic aperture radar(SAR) sensors can detect oil spills on the sea surface. These oil spills appear as dark spots in SAR images. However, dark formations can be caused by a number of phenomena. It is aimed to distinguishing oil spills or look-alike objects. A novel method based on a bidimensional empirical mode decomposition is proposed. The selected dark formations are first decomposed into several bidimensional intrinsic mode functions and the residue. Subsequently, 64 dimension feature sets are calculated using the Hilbert spectral analysis and five new features are extracted with a relief algorithm. Mahalanobis distances are then used for classification. Three data sets containing oil spills or look-alikes are used to test the accuracy rate of the method. The accuracy rate is more than 90%. The experimental results demonstrate that the novel method can detect oil spills validly and accurately.
基金supported by the National Institutes of Health,Grant CA95885.
文摘We have examined ten human subjects with a previously developed instrument for near-infrared diffuse spectral imaging of the female breast.The instrument is based on a tandem,planar scan of two collinear optical fibers(one for illumination and one for collection)to image a gently compressed breast in a transmission geometry.The optical data collection features a spatial sampling of 25 points/cm2 over the whole breast,and a spectral sampling of 2 points/nm in the 650-900nm wavelength range.Of the ten human subjects examined,eight are healthy subjects and two are cancer patients with unilateral invasive ductal carcinoma and ductal carcinoma in situ,respectively.For each subject,we generate second-derivative images that identify a network of highly absorbing structures in the breast that we assign to blood vessels.A previously developed paired-wavelength spectral method assigns oxygenation values to the absorbing structures displayed in the second-derivative images.The resulting oxygenation images feature average values over the whole breast that are significantly lower in cancerous breasts(69±14%,n=2)than in healthy breasts(85±7%,n=18)(p<0.01).Furthermore,in the two patients with breast cancer,the average oxygenation values in the cancerous regions are also significantly lower than in the remainder of the breast(invasive ductal carcinoma:49±11%vs 61±16%,p<0.01;ductal carcinoma in situ:58±8%vs 77±11%,p<0.001).
基金The authors would like to acknowledge the support of the Ph.D.research startup foundation of Guangdong Medical University (2XB14006).
文摘The content of berberine hydrochloride(BH)in compound berberine tablets(CBTs)is subject to strict requirements.Its content is usually measured based on chemical analysis.In this paper,the fluorescence spectral imaging method was used to study the relative content of BH from a physics perspective.By comparing the relative fluorescence intensity of self-made CBTs with di®erent mass percentages of BH,a linear positive relationship was observed between the BH content and the relative fluorescence intensity,and accordingly the quality of CBTs of different brands was evaluated.The results indicate that the fluorescence spectral imaging method can be a simple,fast and nondestructive semi-quantitative analysis method to determine the content of BH in CBTs,and this method has great potential in the quality control of CBTs.
基金supported by the National Natural Science Foundation for Distinguished Young Scholars of China(Grant No.61225024)the National High Technology Research and Development Program of China(Grant No.2011AA7012022)
文摘Coded aperture snapshot spectral imaging(CASSI) has been discussed in recent years. It has the remarkable advantages of high optical throughput, snapshot imaging, etc. The entire spatial-spectral data-cube can be reconstructed with just a single two-dimensional(2D) compressive sensing measurement. On the other hand, for less spectrally sparse scenes,the insufficiency of sparse sampling and aliasing in spatial-spectral images reduce the accuracy of reconstructed threedimensional(3D) spectral cube. To solve this problem, this paper extends the improved CASSI. A band-pass filter array is mounted on the coded mask, and then the first image plane is divided into some continuous spectral sub-band areas. The entire 3D spectral cube could be captured by the relative movement between the object and the instrument. The principle analysis and imaging simulation are presented. Compared with peak signal-to-noise ratio(PSNR) and the information entropy of the reconstructed images at different numbers of spectral sub-band areas, the reconstructed 3D spectral cube reveals an observable improvement in the reconstruction fidelity, with an increase in the number of the sub-bands and a simultaneous decrease in the number of spectral channels of each sub-band.
基金supported by the National Natural Science Foundation of China(No.60508017)the Natural Science Foundation of Fujian Province of China(2007J0007,C0720001)+1 种基金the Science and Technology Planning Key Program of Fujian Province(2008Y0037)the Program for New Century Excellent Talents in University(NCET-07-0191).
文摘Skin scar is unique to humans,the major significant negative outcome sustained after thermal injuries,traumatic injuries,and surgical procedures.Hypertrophic scar in human skin is investigated using non-linear spectral imaging microscopy.The high contrast images and spectroscopic intensities of collagen and elastic fibers extracted from the spectral imaging of normal skin tissue,and the normal skin near and far away from the hypertrophic scar tissues in a 10-year-old patient case are obtained.The results show that there are apparent differences in the morphological structure and spectral characteristics of collagen and elastic fibers when comparing the normal skin with the hypertrophic scar tissue.These differences can be good indicators to differentiate the normal skin and hypertrophic scar tissue and demonstrate that non-linear spectral imaging microscopy has potential to noninvasively investigate the pathophysiology of human hypertrophic scar.
文摘AIM: To determine angle closure agreements between gonioscopy and anterior segment optical coherence tomography(AS-OCT), as well as gonioscopy and spectral domain OCT(SD-OCT). A secondary objective was to quantify inter-observer agreements of AS-OCT and SD-OCT assessments.METHODS: Seventeen consecutive subjects(33 eyes)were recruited from the study hospital’s Glaucoma clinic.Gonioscopy was performed by a glaucomatologist masked to OCT results. OCT images were read independently by 2 other glaucomatologists masked to gonioscopy findings as well as each other’s analyses of OCT images.RESULTS: Totally 84.8% and 45.5% of scleral spurs were visualized in AS-OCT and SD-OCT images respectively(P 【0.01). The agreement for angle closure between AS-OCT and gonioscopy was fair at k =0.31(95% confidence interval, CI: 0.03-0.59) and k =0.35(95%CI: 0.07-0.63) for reader 1 and 2 respectively. The agreement for angle closure between SD-OCT and gonioscopy was fair at k =0.21(95% CI: 0.07-0.49) and slight at k =0.17(95% CI: 0.08-0.42) for reader 1 and 2 respectively. The inter-reader agreement for angle closure in AS-OCT images was moderate at 0.51(95% CI: 0.13-0.88). The inter-reader agreement for angle closure in SD-OCT images was slight at 0.18(95% CI: 0.08-0.45).CONCLUSION: Significant proportion of scleral spurs were not visualised with SD-OCT imaging resulting in weaker inter-reader agreements. Identifying other angle landmarks in SD-OCT images will allow more consistent angle closure assessments. Gonioscopy and OCT imaging do not always agree in angle closure assessments but have their own advantages, and should be used together and not exclusively.
基金This work was supported by the National Council for Science and Technology Grant funded by the Government of Kenya(NCST/ST&I/RCD/4TH call PhD/201).
文摘Neural network analysis based on Growing Hierarchical Self-Organizing Map (GHSOM) is used to examine Spatial-Temporal characteristics in Aerosol Optical Depth (AOD), Ångström Exponent (ÅE) and Precipitation Rate (PR) over selected East African sites from 2000 to 2014. The selected sites of study are Nairobi (1°S, 36°E), Mbita (0°S, 34°E), Mau Forest (0.0° - 0.6°S;35.1°E - 35.7°E), Malindi (2°S, 40°E), Mount Kilimanjaro (3°S, 37°E) and Kampala (0°N, 32.1°E). GHSOM analysis reveals a marked spatial variability in AOD and ÅE that is associated to changing PR, urban heat islands, diffusion, direct emission, hygroscopic growth and their scavenging from the atmosphere specific to each site. Furthermore, spatial variability in AOD, ÅE and PR is distinct since each variable corresponds to a unique level of classification. On the other hand, GHSOM algorithm efficiently discriminated by means of clustering between AOD, ÅE and PR during Long and Short rain spells and dry spell over each variable emphasizing their temporal evolution. The utilization of GHSOM therefore confirms the fact that regional aerosol characteristics are highly variable be it spatially or temporally and as well modulated by PR received over each variable.
基金We acknowledge the financial supports from the National Key R&D Program of China(2021YFF0502900)the National Natural Science Foundation of China(61975033)Shanghai Municipal Science and Technology Major Project No.2018SHZDZX01 and ZJLab.
文摘Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)microscopy is a rapidly growing technique that has attracted broad attentions and become a powerful tool for biology and biomedicine,largely thanks to its chemical specicity,high sensitivity and fast image speed.This review paper introduces the principles of SRS,discusses the technical developments and implementations of SRS microscopy,then highlights and summarizes its applications on biological cellular machinery andnally shares our visions of potential breakthroughs in the future.
基金supported by the National Key Research and Development Program of China(2021YFB3101300,2021YFB3101303)the Natural Science Foundation of China(U22B2030,62302374)+4 种基金Shaanxi Provincial Key Research and Development Program(2023-ZDLGY-35)China Postdoctoral Science Foundation(2022M722498)the Natural Science Basic Research Plan in Shaanxi Province of China(2023-JC-QN-0699)Qin Chuangyuan Cited High-level Innovative and Entrepreneurial Talents Project(QCYRCXM-2022-244)the Science and Technology on Communication Networks Laboratory(HHX23641X003).
文摘With the rapid development of location-based services and online social networks,POI recommendation services considering geographic and social factors have received extensive attention.Meanwhile,the vigorous development of cloud computing has prompted service providers to outsource data to the cloud to provide POI recommendation services.However,there is a degree of distrust of the cloud by service providers.To protect digital assets,service providers encrypt data before outsourcing it.However,encryption reduces data availability,making it more challenging to provide POI recommendation services in outsourcing scenarios.Some privacy-preserving schemes for geo-social-based POI recommendation have been presented,but they have some limitations in supporting group query,considering both geographic and social factors,and query accuracy,making these schemes impractical.To solve this issue,we propose two practical and privacy-preserving geo-social-based POI recommendation schemes for single user and group users,which are named GSPR-S and GSPR-G.Specifically,we first utilize the quad tree to organize geographic data and the MinHash method to index social data.Then,we apply BGV fully homomorphic encryption to design some private algorithms,including a private max/min operation algorithm,a private rectangular set operation algorithm,and a private rectangular overlapping detection algorithm.After that,we use these algorithms as building blocks in our schemes for efficiency improvement.According to security analysis,our schemes are proven to be secure against the honest-but-curious cloud servers,and experimental results show that our schemes have good performance.
基金funding provided by Universitàdi Pisa within the CRUI-CARE Agreement。
文摘Sustainable forest management is essential to confront the detrimental impacts of diseases on forest ecosystems.This review highlights the potential of vegetation spectroscopy in improving the feasibility of assessing forest disturbances induced by diseases in a timely and cost-effective manner.The basic concepts of vegetation spectroscopy and its application in phytopathology are first outlined then the literature on the topic is discussed.Using several optical sensors from leaf to landscape-level,a number of forest diseases characterized by variable pathogenic processes have been detected,identified and quantified in many country sites worldwide.Overall,these reviewed studies have pointed out the green and red regions of the visible spectrum,the red-edge and the early near-infrared as the spectral regions most sensitive to the disease development as they are mostly related to chlorophyll changes and symptom development.Late disease conditions particularly affect the shortwave-infrared region,mostly related to water content.This review also highlights some major issues to be addressed such as the need to explore other major forest diseases and geographic areas,to further develop hyperspectral sensors for early detection and discrimination of forest disturbances,to improve devices for remote sensing,to implement longterm monitoring,and to advance algorithms for exploitation of spectral data.Achieving of these goals will enhance the capability of vegetation spectroscopy in early detection of forest stress and in managing forest diseases.
基金The National Natural Science Foundation of China(Grant No.U22A6004)The National Key Research and Development Program of China(2022YFF1501600).
文摘Spectral imaging extends the concept of traditional color cameras to capture images across multiple spectral channels and has broad ap-plication prospects.Conventional spectral cameras based on scanning methods suffer from the drawbacks of low acquisition speed and large volume.On-chip computational spectral imaging based on metasur-face filters provides a promising scheme for portable applications,but endures long computation time due to point-by-point iterative spec-tral reconstruction and mosaic effect in the reconstructed spectral im-ages.In this study,on-chip rapid spectral imaging was demonstrated,which eliminated the mosaic effect in the spectral image by deep-learning-based spectral data cube reconstruction.The experimental results show that 4 orders of magnitude faster than the iterative spec-tral reconstruction were achieved,and the fidelity of the spectral re-construction for the standard color plate was over 99%for a standard color board.In particular,video-rate spectral imaging was demon-strated for moving objects and outdoor driving scenes with good per-formance for recognizing metamerism,where the concolorous sky and white cars can be distinguished via their spectra,showing great po-tential for autonomous driving and other practical applications in the field of intelligent perception.
文摘AIM: To evaluate whether virtual chromoendoscopy can improve the delineation of small bowel lesions previously detected by conventional white light small bowel capsule endoscopy(SBCE). METHODS: Retrospective single center study. One hundred lesions selected from forty-nine consecutive conventional white light SBCE(SBCE-WL) examinations were included. Lesions were reviewed at three Flexible Spectral Imaging Color Enhancement(FICE) settings and Blue Filter(BF) by two gastroenterologists with ex-perience in SBCE, blinded to each other's findings, whoranked the quality of delineation as better, equivalent or worse than conventional SBCE-WL. Inter-observer percentage of agreement was determined and analyzed with Fleiss Kappa(k) coefficient. Lesions selected for the study included angioectasias(n = 39), ulcers/ero-sions(n = 49) and villous edema/atrophy(n = 12). RESULTS: Overall, the delineation of lesions was im-proved in 77% of cases with FICE 1, 74% with FICE 2, 41% with FICE 3 and 39% with the BF, with a percent-age of agreement between investigators of 89%(k = 0.833), 85%(k = 0.764), 66%(k = 0.486) and 79%(k = 0.593), respectively. FICE 1 improved the delineation of 97.4% of angioectasias, 63.3% of ulcers/erosions and 66.7% of villous edema/atrophy with a percentage of agreement of 97.4%(k = 0.910), 81.6%(k = 0.714) and 91.7%(k = 0.815), respectively. FICE 2 improved the delineation of 97.4% of angioectasias, 57.1% of ulcers/erosions and 66.7% of villous edema/atrophy, with a percentage of agreement of 89.7%(k = 0.802), 79,6%(k = 0.703) and 91.7%(k = 0.815), respectively. FICE 3 improved the delineation of 46.2% of angioecta-sias, 24.5% of ulcers/erosions and none of the cases of villous edema/atrophy, with a percentage of agreement of 53.8% [k = not available(NA)], 75.5%(k = NA) and 66.7%(k = 0.304), respectively. The BF improved the delineation of 15.4% of angioectasias, 61.2% of ulcers/erosions and 25% of villous edema/atrophy, with a per-centage of agreement of 76.9%(k = 0.558), 81.6%(k = 0.570) and 25.0%(k = NA), respectively.CONCLUSION: Virtual chromoendoscopy can improve the delineation of angioectasias, ulcers/erosions and villous edema/atrophy detected by SBCE, with almost perfect interobserver agreement for FICE 1.