It remains unclear whether spinal cord ischemia-reperfusion injury caused by ischemia and other non-mechanical factors can be monitored by somatosensory evoked potentials. Therefore, we monitored spinal cord ischemia-...It remains unclear whether spinal cord ischemia-reperfusion injury caused by ischemia and other non-mechanical factors can be monitored by somatosensory evoked potentials. Therefore, we monitored spinal cord ischemia-reperfusion injury in rabbits using somatosensory evoked potential detection technology. The results showed that the somatosensory evoked potential latency was significantly prolonged and the amplitude significantly reduced until it disappeared during the period of spinal cord ischemia. After reperfusion for 30-180 minutes, the amplitude and latency began to gradually recover; at 360 minutes of reperfusion, the latency showed no significant difference compared with the pre-ischemic value, while the somatosensory evoked potential amplitude in- creased, and severe hindlimb motor dysfunctions were detected. Experimental findings suggest that changes in somatosensory evoked potentia~ ~atency can reflect the degree of spinat cord ischemic injury, while the amplitude variations are indicators of the late spinal cord reperfusion injury, which provide evidence for the assessment of limb motor function and avoid iatrogenic spinal cord injury.展开更多
The spinal cord is at risk of injury during spinal surgery.If intraoperative spinal co rd injury is identified early,irreve rsible impairment or loss of neurological function can be prevented.Different types of spinal...The spinal cord is at risk of injury during spinal surgery.If intraoperative spinal co rd injury is identified early,irreve rsible impairment or loss of neurological function can be prevented.Different types of spinal cord injury result in damage to diffe rent spinal cord regions,which may cause diffe rent somatosensory and motor evoked potential signal res ponses.In this study,we examined electrophysiological and histopathological changes between contusion,distra ction,and dislocation spinal cord injuries in a rat model.We found that contusion led to the most severe dorsal white matter injury and caused considerable attenuation of both somatosensory and motor evoked potentials.Dislocation resulted in loss of myelinated axons in the lateral region of the injured spinal cord along the rostrocaudal axis.The amplitude of attenuation in motor evoked potential responses caused by dislocation was greater than that caused by contusion.After distraction injury,extracellular spaces were slightly but not significantly enlarged;somatosensory evoked potential res ponses slightly decreased and motor evoked potential responses were lost.Correlation analysis showed that histological and electrophysiological findings we re significantly correlated and related to injury type.Intraope rative monitoring of both somatosensory and motor evoked potentials has the potential to identify iatrogenic spinal cord injury type during surgery.展开更多
Existing work indicates that the degree of variation of somatosensory evoked potential (SEP) signals between a healthy spinal pathway and spinal pathway affected by spinal cord injury (SCI) can be used to evaluate the...Existing work indicates that the degree of variation of somatosensory evoked potential (SEP) signals between a healthy spinal pathway and spinal pathway affected by spinal cord injury (SCI) can be used to evaluate the integrity of the spinal pathway. This paper develops a metric that exploits the time-domain features of SEP signals (relative amplitude, time scaling, and time duration) in order to quantify the level of SCI. The proposed method is tested on actual SEP signals collected from rodents afflicted with focal demyelination SCI. Results indicate that the proposed method provides a robust assessment of the different degrees of demyelination in the spinal cord.展开更多
Stem cell transplantation can promote functional restoration following acute spinal cord injury （injury time 〈 3 months）, but the safety and long-term efficacy of this treatment need further exploration. In this st...Stem cell transplantation can promote functional restoration following acute spinal cord injury （injury time 〈 3 months）, but the safety and long-term efficacy of this treatment need further exploration. In this study, 25 patients with traumatic spinal cord injury （injury time 〉 6 months） were treated with human umbilical cord blood stem cells via intravenous and intrathecal injection. The follow-up period was 12 months after transplantation. Results found that autonomic nerve functions were restored and the latent period of somatosensory evoked potentials was reduced. There were no severe adverse reactions in patients following stem cell transplantation. These experimental findings suggest that the transplantation of human umbilical cord blood stem cells is a safe and effective treatment for patients with traumatic spinal cord injury展开更多
Although electrical stimulation is therapeutically applied for neural regeneration in patients, it remains unclear how electrical stimulation exerts its effects at the molecular level on spinal cord injury （SCI）. To...Although electrical stimulation is therapeutically applied for neural regeneration in patients, it remains unclear how electrical stimulation exerts its effects at the molecular level on spinal cord injury （SCI）. To identify the signaling pathway involved in electrical stimulation improving the function of injured spinal cord, 21 female Sprague-Dawley rats were randomly assigned to three groups： control （no surgical intervention, n = 6）, SCI （SCI only, n = 5）, and electrical simulation （ES; SCI induction followed by ES treatment, n = 10）. A complete spinal cord transection was performed at the 10^th thoracic level. Electrical stimulation of the injured spinal cord region was applied for 4 hours per day for 7 days. On days 2 and 7 post SCI, the Touch-Test Sensory Evaluators and the Basso-Beattie-Bresnahan locomotor scale were used to evaluate rat sensory and motor function. Somatosensory-evoked potentials of the tibial nerve of a hind paw of the rat were measured to evaluate the electrophysiological function of injured spinal cord. Western blot analysis was performed to measure p38-RhoA and ERK1/2-Bcl-2 pathways related protein levels in the injured spinal cord. Rat sensory and motor functions were similar between SCI and ES groups. Com- pared with the SCI group, in the ES group, the latencies of the somatosensory-evoked potential of the tibial nerve of rats were significantly shortened, the amplitudes were significantly increased, RhoA protein level was significantly decreased, protein gene product 9.5 expression, ERK1/2, p38, and Bcl-2 protein levels in the spinal cord were significantly increased. These data suggest that ES can promote the recovery of electrophysiological function of the injured spinal cord through regulating p38-RhoA and ERK1/2-Bcl-2 pathway-related protein levels in the injured spinal cord.展开更多
Somatosensory evoked potentials(SEPs)have been widely used to assess neurological function in clinical practice.A good understanding of the association between SEP signals and neurological function is helpful for prec...Somatosensory evoked potentials(SEPs)have been widely used to assess neurological function in clinical practice.A good understanding of the association between SEP signals and neurological function is helpful for precise diagnosis of impairment location.Previous studies on SEPs have been reported in animal models.However,few studies have reported the relationships between SEP waveforms in animals and those in humans.In this study,we collected normal SEP waveforms and decomposed them into specific time–frequency components(TFCs).Our results showed three stable TFC distribution regions in intact goats and rats and in humans.After we induced spinal cord injury in the animal models,a greater number of small TFC distribution regions were observed in the injured goat and rat groups than in the normal group.Moreover,there were significant correlations(P<0.05)and linear relationships between the main SEP TFCs of the human group and those of the goat and rat groups.A stable TFC distribution of SEP components was observed in the human,goat and rat groups,and the TFC distribution modes were similar between the three groups.Results in various animal models in this study could be translated to future clinical studies based on SEP TFC analysis.Human studies were approved by the Institutional Review Board of the University of Hong Kong/Hospital Authority Hong Kong West Cluster(approval No.UM 05-312 T/975)on December 5,2005.Rat experiments were approved by the Committee on the Use of Live Animals in Teaching and Research of Li Ka Shing Faculty of Medicine of the University of Hong Kong(approval No.CULART 2912-12)on January 28,2013.Goat experiments were approved by the Animal Ethics Committee of Affiliated Hospital of Guangdong Medical University(approval No.GDY2002132)on March 5,2018.展开更多
The latencies of motor- and somatosensory-evoked potentials were prolonged to different degrees, and wave amplitude was obviously decreased, after injection of dynorphin into the rat subarachnoid cavity. The wave ampl...The latencies of motor- and somatosensory-evoked potentials were prolonged to different degrees, and wave amplitude was obviously decreased, after injection of dynorphin into the rat subarachnoid cavity. The wave amplitude and latencies of motor- and somatosensory-evoked potentials were significantly recovered at 7 and 14 days after combined injection of dynorphin and either the kappa opioid receptor antagonist nor-binaltorphimine or the N-methyl-D-aspartate receptor antagonist MK-801. The wave amplitude and latency were similar in rats after combined injection of dynorphin and nor-binaltorphimine or MK-801. These results suggest that intrathecal injection of dynorphin causes damage to spinal cord function. Prevention of N-methyl-D-aspartate receptor or kappa receptor activation lessened the injury to spinal cord function induced by dynorphin.展开更多
目的比较不同肌松水平[4个成串刺激(train of four stimulation,TOF)的T1分别为5%~15%基础值和45%~55%基础值水平]对脊柱手术中脊髓神经电生理监测结果的影响,探讨安全有效的电生理监测麻醉方案。方法选择行术中脊髓神经电生理监测的择...目的比较不同肌松水平[4个成串刺激(train of four stimulation,TOF)的T1分别为5%~15%基础值和45%~55%基础值水平]对脊柱手术中脊髓神经电生理监测结果的影响,探讨安全有效的电生理监测麻醉方案。方法选择行术中脊髓神经电生理监测的择期脊柱手术病人23例。采用丙泊酚和瑞芬太尼全凭静脉麻醉,阿曲库铵维持肌松,监测拇内收肌TOF指示肌松水平,监测体感诱发电位(somatosensory evoked potentials,SEP)和运动诱发电位(motor evoked potentials,MEP)评判脊髓功能。分别记录神经肌肉阻滞水平1(neuromuscular blockade level 1,NMB_1)(T1为5%~15%基础值)和NMB_2水平(T1为45%~55%基础值)时SEP和MEP的波幅和潜伏期,同时记录经颅电刺激时病人是否出现剧烈体动和自主呼吸。结果不同肌松水平的SEP波幅和潜伏期之间差异均无统计学意义(P>0.05)。同一监测部位不同肌松水平的MEP潜伏期差异无统计学意义(P>0.05),左上肢和右下肢不同肌松水平的MEP波幅则差异有统计学意义(P<0.05)。NMB2水平时的经颅电刺激时剧烈体动发生率明显高于NMB1水平时(P<0.05)。两个肌松水平经颅电刺激时均无自主呼吸产生。结论肌松剂的使用在行神经电生理监测的脊柱手术中并非完全禁忌,TOF的T1在45%~55%基础值的肌松水平可能是高风险脊髓手术较理想的肌松水平。展开更多
基金supported by the National Natural Science Foundation of China,No.81101370,81101399,81272018the Natural Science Foundation of Jiangsu Province in China,No.BK2011303+2 种基金Jiangsu Province Science and Technology Support Program(Social Development)in China,No.BE2011672University Natural Science Research Foundation of Jiangsu Province for Higher Education,No.12KJB320008College Graduate Research and Innovation Plan of Jiangsu Province in China,No.CXZZ11_0126
文摘It remains unclear whether spinal cord ischemia-reperfusion injury caused by ischemia and other non-mechanical factors can be monitored by somatosensory evoked potentials. Therefore, we monitored spinal cord ischemia-reperfusion injury in rabbits using somatosensory evoked potential detection technology. The results showed that the somatosensory evoked potential latency was significantly prolonged and the amplitude significantly reduced until it disappeared during the period of spinal cord ischemia. After reperfusion for 30-180 minutes, the amplitude and latency began to gradually recover; at 360 minutes of reperfusion, the latency showed no significant difference compared with the pre-ischemic value, while the somatosensory evoked potential amplitude in- creased, and severe hindlimb motor dysfunctions were detected. Experimental findings suggest that changes in somatosensory evoked potentia~ ~atency can reflect the degree of spinat cord ischemic injury, while the amplitude variations are indicators of the late spinal cord reperfusion injury, which provide evidence for the assessment of limb motor function and avoid iatrogenic spinal cord injury.
基金supported by the National Natural Science Foundation of China,No.81871768(to YH)Natural Science Foundation of Tianjin,China,No.18JCYBJC29600(to HYC)High Level-Hospital Program,Health Commission of Guangdong Province,China,No.HKUSZH201902011(to YH)。
文摘The spinal cord is at risk of injury during spinal surgery.If intraoperative spinal co rd injury is identified early,irreve rsible impairment or loss of neurological function can be prevented.Different types of spinal cord injury result in damage to diffe rent spinal cord regions,which may cause diffe rent somatosensory and motor evoked potential signal res ponses.In this study,we examined electrophysiological and histopathological changes between contusion,distra ction,and dislocation spinal cord injuries in a rat model.We found that contusion led to the most severe dorsal white matter injury and caused considerable attenuation of both somatosensory and motor evoked potentials.Dislocation resulted in loss of myelinated axons in the lateral region of the injured spinal cord along the rostrocaudal axis.The amplitude of attenuation in motor evoked potential responses caused by dislocation was greater than that caused by contusion.After distraction injury,extracellular spaces were slightly but not significantly enlarged;somatosensory evoked potential res ponses slightly decreased and motor evoked potential responses were lost.Correlation analysis showed that histological and electrophysiological findings we re significantly correlated and related to injury type.Intraope rative monitoring of both somatosensory and motor evoked potentials has the potential to identify iatrogenic spinal cord injury type during surgery.
文摘Existing work indicates that the degree of variation of somatosensory evoked potential (SEP) signals between a healthy spinal pathway and spinal pathway affected by spinal cord injury (SCI) can be used to evaluate the integrity of the spinal pathway. This paper develops a metric that exploits the time-domain features of SEP signals (relative amplitude, time scaling, and time duration) in order to quantify the level of SCI. The proposed method is tested on actual SEP signals collected from rodents afflicted with focal demyelination SCI. Results indicate that the proposed method provides a robust assessment of the different degrees of demyelination in the spinal cord.
文摘Stem cell transplantation can promote functional restoration following acute spinal cord injury （injury time 〈 3 months）, but the safety and long-term efficacy of this treatment need further exploration. In this study, 25 patients with traumatic spinal cord injury （injury time 〉 6 months） were treated with human umbilical cord blood stem cells via intravenous and intrathecal injection. The follow-up period was 12 months after transplantation. Results found that autonomic nerve functions were restored and the latent period of somatosensory evoked potentials was reduced. There were no severe adverse reactions in patients following stem cell transplantation. These experimental findings suggest that the transplantation of human umbilical cord blood stem cells is a safe and effective treatment for patients with traumatic spinal cord injury
基金supported by a grant from Wonkwang Institute of Clinical Medicine in 2011
文摘Although electrical stimulation is therapeutically applied for neural regeneration in patients, it remains unclear how electrical stimulation exerts its effects at the molecular level on spinal cord injury （SCI）. To identify the signaling pathway involved in electrical stimulation improving the function of injured spinal cord, 21 female Sprague-Dawley rats were randomly assigned to three groups： control （no surgical intervention, n = 6）, SCI （SCI only, n = 5）, and electrical simulation （ES; SCI induction followed by ES treatment, n = 10）. A complete spinal cord transection was performed at the 10^th thoracic level. Electrical stimulation of the injured spinal cord region was applied for 4 hours per day for 7 days. On days 2 and 7 post SCI, the Touch-Test Sensory Evaluators and the Basso-Beattie-Bresnahan locomotor scale were used to evaluate rat sensory and motor function. Somatosensory-evoked potentials of the tibial nerve of a hind paw of the rat were measured to evaluate the electrophysiological function of injured spinal cord. Western blot analysis was performed to measure p38-RhoA and ERK1/2-Bcl-2 pathways related protein levels in the injured spinal cord. Rat sensory and motor functions were similar between SCI and ES groups. Com- pared with the SCI group, in the ES group, the latencies of the somatosensory-evoked potential of the tibial nerve of rats were significantly shortened, the amplitudes were significantly increased, RhoA protein level was significantly decreased, protein gene product 9.5 expression, ERK1/2, p38, and Bcl-2 protein levels in the spinal cord were significantly increased. These data suggest that ES can promote the recovery of electrophysiological function of the injured spinal cord through regulating p38-RhoA and ERK1/2-Bcl-2 pathway-related protein levels in the injured spinal cord.
基金supported by the National Natural Science Foundation of China,No.81871768(to YH)the Natural Science Foundation of Tianjin of China,No.18JCYBJC29600(to HYC)High Level-Hospital Program,Health Commission of Guangdong Province of China,No.HKUSZH201902011(to YH).
文摘Somatosensory evoked potentials(SEPs)have been widely used to assess neurological function in clinical practice.A good understanding of the association between SEP signals and neurological function is helpful for precise diagnosis of impairment location.Previous studies on SEPs have been reported in animal models.However,few studies have reported the relationships between SEP waveforms in animals and those in humans.In this study,we collected normal SEP waveforms and decomposed them into specific time–frequency components(TFCs).Our results showed three stable TFC distribution regions in intact goats and rats and in humans.After we induced spinal cord injury in the animal models,a greater number of small TFC distribution regions were observed in the injured goat and rat groups than in the normal group.Moreover,there were significant correlations(P<0.05)and linear relationships between the main SEP TFCs of the human group and those of the goat and rat groups.A stable TFC distribution of SEP components was observed in the human,goat and rat groups,and the TFC distribution modes were similar between the three groups.Results in various animal models in this study could be translated to future clinical studies based on SEP TFC analysis.Human studies were approved by the Institutional Review Board of the University of Hong Kong/Hospital Authority Hong Kong West Cluster(approval No.UM 05-312 T/975)on December 5,2005.Rat experiments were approved by the Committee on the Use of Live Animals in Teaching and Research of Li Ka Shing Faculty of Medicine of the University of Hong Kong(approval No.CULART 2912-12)on January 28,2013.Goat experiments were approved by the Animal Ethics Committee of Affiliated Hospital of Guangdong Medical University(approval No.GDY2002132)on March 5,2018.
基金Key Science and Technology Research and Development Program of Liaoning Province, China, No. 20112250021, 20112250041.
文摘The latencies of motor- and somatosensory-evoked potentials were prolonged to different degrees, and wave amplitude was obviously decreased, after injection of dynorphin into the rat subarachnoid cavity. The wave amplitude and latencies of motor- and somatosensory-evoked potentials were significantly recovered at 7 and 14 days after combined injection of dynorphin and either the kappa opioid receptor antagonist nor-binaltorphimine or the N-methyl-D-aspartate receptor antagonist MK-801. The wave amplitude and latency were similar in rats after combined injection of dynorphin and nor-binaltorphimine or MK-801. These results suggest that intrathecal injection of dynorphin causes damage to spinal cord function. Prevention of N-methyl-D-aspartate receptor or kappa receptor activation lessened the injury to spinal cord function induced by dynorphin.
