New spinosin derivatives from the seeds of Ziziphus mauritiana

Three new acylated flavonoid C-glycosides,6'''-(-)-phaseoylspinosin(1),6'''-(3'''',4'''',5''''-trimethoxyl)-(E)-cinnamoylspinosin(2),and 6'''-(4''''-O-β-D-gluco-pyranosyl)-benzoylspinosin(3),were isolated from the seeds of Ziziphus mauritiana(Rhamnaceae).A further 19 known compounds including eight spinosin analogues(4-11)were also isolated.Their structures were elucidated by means of spectroscopic analysis and chemical method.Among spinosin derivatives 1,2,4,7,8,and triterpenoid saponin 14,jujuboside A(14)displayed moderate acetylcholinesterase(AchE)inhibitory activity with an inhibition value of 46.2%at a concentration of 1μM.

Inhibitory effects of jujuboside A on EEG and hippocampal glutamate in hyperactive rat

In this study, the inhibitory effect of jujuboside A (JuA) on a penicillin sodium (Na-PCN) induced hyperactivity model was investigated. Cortical EEG (electroencephalogram) and the concentration of hippocampal Glutamate (Glu) were monitored simultaneously in vivo as indicators of rat’s excitatory state. Power spectral density (PSD) and gravity frequency of PSD were calculated. JuA (0.05 g/L and 0.1 g/L) inhibited the EEG excitation effect caused by Na-PCN by increasing the power of δ1 and δ2 bands (P<0.01 vs model) and lowering the gravity frequency of PSD (P<0.01 vs model). JuA also remarkably reduced the Glu elevation induced by Na-PCN (P<0.05 vs model). Diazepam also depressed Glu concentration and lowered the gravity frequency, but it showed a different EEG pattern in increased β2-activity (P<0.01 vs model). EEG excitation caused by Na-PCN correlated with Glu elevation during the first hour. Neurophysiological inhibitory effects of JuA and diazepam were more persistent than their Glu inhibitoty effects.

The effect of Jujuboside A on the evoked field potentials of Granule cells in dentate gyrus

Jujuboside A( JuA) is a main component of Jujubogenin extracted from the seeds of Ziziphus.The authors have not seen report on JuA's direct effect on the neruons of the central nervous system.This study aimed to assess the effect of JuA on paried-pulse responses of dentate gyrus granule cells in urethaneanasesthetized rats,used intracerebroventricular(i.c.v.) JuA to mimic in vitro tath conditions in vivo.Pariedpulse stimuli with 80ms interpulse interval were used to stimulate the perforant pathway.Evoked responses first responses,the slopoes of excitatory postsynaptic potential(EPSP1) and the amplitudes of population spike (PS1) decreased significantly after administration of JuA while the PS1 latencies increased significantly.In the second responses.the EPSP2 slops and PS2 latencies were changed similarly to those of the first ones.but PS2 amplitudes increased.The results showed that JuA may have some inhibitory effect on the granule cell excitability mediated by presynaptic mechanism but may have little effect on the excitability mediated by postsynaptic mechanism since the second evoked N-methyl-D-aspartic mediating paired-pulse facilitation is a postsynaptic mechanism.

酸枣仁主要成分Spinosin催眠作用及其机制研究

目的：探讨酸枣仁主要成分Spinosin的催眠作用以及对戊巴比妥钠诱导的大鼠睡眠时相的影响，进而研究其作用机制。方法：采用戊巴比妥钠诱导的小鼠翻正反射实验和大鼠脑电（EEG）分析技术。结果：Spinosin能够显著缩短催眠剂量戊巴比妥钠（45mg／kg，i．p．）诱导的小鼠睡眠潜伏期，延长睡眠时间，同时可以显著增加阈下剂量（28mg／kg，i．p．）戊巴比妥钠实验中的小鼠入睡率。

Jujuboside a Improved Energy Metabolism in Senescent H9c2 Cells Injured by Ischemia, Hypoxia, and Reperfusion through the CD38/Silent Mating Type Information Regulation 2 Homolog 3 Signaling Pathway

Objective:This study explored the myocardial protection role of Jujuboside A through an ischemia–hypoxia–reperfusion(IHR)model.Materials and Methods:H9c2 cells were induced by D-galactose(D-gal)and IHR to establish an aging and IHR model.There are four groups of experiments:Control,IHR,D-gal+IHR,and D-gal+IHR+Jujuboside A.Cells viability,Adenosine triphosphate(ATP),reactive oxygen species(ROS),nicotinamide adenine dinucleotide(NAD+),nicotinamide adenine dinucleotide hydride(NADH)content,and NAD+/NADH ratio were detected using biochemical methods.Inflammatory cytokines level was detected by enzyme-linked immunosorbent assay.The expression of CD38,Recombinant NLR Family,pyrin domain-containing protein 3(NLRP3),and silent mating type information regulation 2homolog 3(SIRT3)protein was detected by Western blotting.Results:Compared to the IHR group,cell viability,ATP content,NAD+content,NAD+/NADH ratio,and SIRT3 protein expression decreased,ROS level and inflammatory cytokines increased,and CD38 and NLRP3 proteins raised in the D-gal+IHR group.Compared to the D-gal+IHR group,cell viability,ATP content,NAD+content,NAD+/NADH ratio,and expression of SIRT3 protein increased,ROS level and inflammatory cytokines level decreased,and expression of the CD38 and NLRP3proteins decreased in the D-gal+IHR+Jujuboside A group.Conclusions:Jujuboside A inhibited the expression of CD38,improved energy metabolism disorder,and mitochondrial function,and decreased inflammation in D-gal-induced H9c2 cells.

Spinosin protects Neuro-2a/APP695 cells from oxidative stress damage by inactivating p38

OBJECTIVE:To explore the protective mechanism of spinosin(SPI)on Alzheimer's disease(AD)model cells,Neuro-2a/APP695(N2a/APP695),against H_(2)O_(2)-induced oxidative stress damage,to reflect the influence of oxidative stress on the development of AD,and to provide a valuable basis for the research and development of therapeutic drug for AD.METHODS:N2a/APP695 cells were exposed to H2O2 and then treated with spinosin.Firstly,the secretion level of amyloidβ(Aβ)1-42 and the production of malondialdehyde(MDA)and lactate dehydrogenase(LDH)were detected by enzyme linked immunosorbent assay kits.Secondly,the oligomerization degree of Aβ1-42 was performed by Thioflavin T staining.Thirdly,the expression levels of p-Tau(Ser199/202/396),synaptophysin(SYP),postsynaptic density protein 95(PSD95),and mitogen-activated protein kinase(MAPK)family-related proteins were detected by Western blot analysis.In addition,FITC-labeled phalloidin was used in cytoskeleton staining to reflect synaptic function.RESULTS:This study showed that H2O2 stimulated N2a/APP695 cells to produce excessive MDA and LDH and secrete a large amount of Aβ,promoted the aggregation of Aβ,induced Tau protein hyperphosphorylation,and led to synaptic dysfunction.Spinosin reversed these changes caused by H2O2 by inactivating p38,which was verified by treatment with the p38 inhibitor BIRB796.CONCLUSION:Spinosin protects N2a/APP695 cells from oxidative stress damage caused by H2O2 through inactivating p38.

酸枣仁皂苷A对抑郁小鼠行为及突触可塑性相关蛋白的作用

该文探究了酸枣仁皂苷A(Jujuboside A,JuA)对抑郁模型小鼠行为及神经突触可塑性相关蛋白表达的作用。通过悬尾实验、强迫游泳实验、旷场实验、Morris水迷宫实验评价小鼠的抑郁状态,免疫组织化学染色法检测小鼠海马组织中脑源性神经营养因子(Brain Derived Neurotrophic Factor,BDNF)的表达水平,Western blot实验检测小鼠海马组织酪氨酸激酶受体B(Tyrosine Kinase Receptor B,TrkB)、cAMP反应元件结合蛋白(cAMP Responsive Element Binding,CREB)、突触后密度蛋白95(Postsynaptic Density Protein 95,PSD95)、自噬效应蛋白Beclin1(Autophagy Effector Protein Beclin1,Beclin1)的表达水平。结果表明,JuA显著改善抑郁模型小鼠的抑郁状态。同时,JuA作用后海马组织BDNF、TrkB、CREB、PSD95、Beclin1的表达水平较模型组分别上调了190.23%、137.24%、76.29%、169.32%、82.53%。综上所述,JuA对抑郁模型小鼠具有显著的抗抑郁作用,并能明上调BDNF、TrkB、CREB、PSD95、Beclin1等神经突触可塑性相关蛋白的表达。实验结果为JuA在抑郁症临床治疗方面的应用提供理论依据,并为探究抑郁症药物治疗靶点提供参考。

酸枣仁皂苷A通过miR-223-3p/SGK1/NLRP3轴在脓毒症小鼠肺上皮细胞损伤中的保护作用

目的 探讨酸枣仁皂苷A通过miR-223-3p/血清和糖皮质激素诱导激酶1(SGK1)/NOD样受体热蛋白结构域相关蛋白3(NLRP3)轴在脓毒症小鼠肺上皮细胞损伤中的保护作用及潜在机制。方法 通过盲肠结扎和穿刺(CLP)诱导脓毒症动物模型。通过酸枣仁皂苷A治疗实验动物,苏木精和伊红染色分析组织病理学变化。相应试剂盒分析肾损伤指标(血清BUN、SCr和NGAL水平)和血清炎性因子(TNF-α、IL-1β、IL-6)的水平。生信分析miR-223-3p在脓毒症患者中的表达及其与炎症反应因子的相关性,预测miR-223-3p与SGK1的结合位点。双荧光素酶实验验证miR-223-3p与SGK1的结合关系。构建脂多糖(LPS)诱导的HK2细胞的体外模型。荧光实时定量PCR(qPCR)检测miR-223-3p在患者血清或LPS处理的HK-2细胞中的表达。脱氧核苷酸末端转移酶介导的dUTP缺口末端标记(TUNEL)检测细胞凋亡,蛋白质免疫印迹检测中BCL2相关的X(BAX)、B细胞淋巴瘤-2(Bcl-2)和裂解的半胱氨酸蛋白酶(cleaved caspase-3)的表达,酶联免疫吸附试验(ELISA)检测患者血清或细胞上清液中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素6(IL-6)的水平。qPCR和蛋白质免疫印迹法检测不同处理组HK-2细胞中SGK1的表达,蛋白质免疫印迹检测不同处理组HK-2细胞中NLRP3的表达。结果 酸枣仁皂苷A治疗可以降低脓毒症小鼠急性肾小管坏死评分,减轻肾损伤。血清血尿素氮(BUN)、血肌酐(Scr)和中性粒细胞明胶酶相关脂质运载蛋白(NGAL)水平降低,血清TNF-α、IL-1β、IL-6水平亦下降。酸枣仁皂苷A治疗可以降低脓毒症小鼠肾脏组织中miR-223-3p的表达水平。生信分析和临床样本分析显示,miR-223-3p在脓毒症患者中高表达。细胞实验结果显示,miR-223-3p在LPS处理的HK-2细胞中呈高表达。miR-223-3p促进LPS诱导的HK-2细胞凋亡和炎症反应,这种促进作用是由miR-223-3p通过负调控SGK1/NLRP3介导的。结论 酸枣仁皂苷A可能通过miR-223-3p/SGK1/NLRP3轴在脓毒症小鼠肺上皮细胞损伤中发挥保护作用,可能为脓毒症治疗提供新的治疗靶点。

光谱法、量热法以及分子对接研究酸枣仁皂苷A与中心蛋白的作用

目的研究酸枣仁皂苷A(jujuboside A,JuA)与八肋游仆虫中心蛋白(Euplotes octocarinatus centrin,EoCen)的相互作用。方法采用圆二色光谱(circular dichroism spectroscopy,CD)以及三维荧光光谱研究EoCen的构象变化;采用稳态荧光光谱与等温滴定量热法(isothermal titration calorimetry,ITC)研究JuA与EoCen作用的机制;分子对接技术模拟JuA与EoCen的结合位点。结果JuA以摩尔比1:1结合于EoCen的C端(二者反应的结合常数约为10^(5)L/mol),由ITC拟合结果可知,JuA与EoCen复合物的形成是放热的过程,熵变对自由能的贡献较小,主要由焓变驱动。EoCen与JuA结合后,构象发生变化,α-螺旋含量减少,酪氨酸残基的微环境变化,EoCen的C端不再结合蜂毒素。结论JuA主要通过范德华力与EoCen结合,从而改变EoCen的构象,抑制其与靶蛋白的结合。

Jujuboside A ameliorates tubulointerstitial fibrosis in diabetic mice through down-regulating the YY1/TGF-β1 signaling pathway

Diabetic nephropathy(DN)is one of the most common complications of diabetes mellitus,which is characterized in renal tubulointerstitial fibrosis(TIF).The current study was designed to investigate the protective effect of Jujuboside A(Ju A)on TIF in type 2 diabetes(T2DM)mice,and explore its underlying anti-fibrosis mechanism.A mouse T2DM model was established using high fat diet(HFD)feeding combined with intraperitoneal injection of streptozotocin(STZ).Then,diabetic mice were treated with Ju A(10,20 and 40 mg·kg^(−1)·d^(−1),i.g.)for 12 weeks.Results showed that administration of Ju A not only down-regulated fasting blood glucose(FBG)levels,but also improved hyperlipidemia and renal function in diabetic mice.Moreover,the reduced ECM accumulation was observed in the renal cortex of Ju A treated diabetic mice,while the TIF progression was also attenuated by Ju A through blocking the epithelial-to-mesenchymal transition(EMT)of renal tubular epithelial cells(RTECs).Further mechanism studies showed that Ju A treatment effectively down-regulated the protein expression and subsequent nuclear translocation of Yin Yang 1(YY1)in the renal cortex of diabetic mice,and reduced the levels of transforming growth factor-β1(TGF-β1)in the serum and renal cortex of Ju A treated mice.According to in vitro studies,the up-regulated YY1/TGF-β1 signaling pathway was restored by Ju A in high glucose(HG)cultured HK-2 cells.Taken together,these findings demonstrated that Ju A can ameliorate the TIF of DN through down-regulating the YY1/TGF-β1 signaling pathway.

Jujuboside A inhibits oxidative stress damage and enhances immun-omodulatory capacity of human umbilical cord mesenchymal stem cells through up-regulating IDO expression

Impaired immunomodulatory capacity and oxidative stress are the key factors limiting the effectiveness of mesenchymal stem cell transplantation therapy.The present study was aimed to investigate the effects of jujuboside A(JuA)on the protective effect and immunomodulatory capacity of human umbilical cord mesenchymal stem cells(hUC-MSCs).Hydrogen peroxide was used to establish an oxidative damage model of hUC-MSCs,while PBMCs isolated from rats were used to evaluate the effect of JuA pre-treatment on the immunomodulatory capacity of hUC-MSCs.Furthermore,Hoechst 33258 staining,lactate dehydrogenase test,measurement of malondialdehyde,Western blot,high-performance liquid chromatography;and flow cytometry were performed.Our results indicated that JuA(25μmol·L−1)promoted the proliferation of hUC-MSCs,but did not affect the differentiating capability of these cells.JuA pre-treatment inhibited apoptosis,prevented oxidative damage,and up-regulated the protein expression of nuclear factor-erythroid factor 2-related factor 2 and heme oxygenase 1 in hUC-MSCs in which oxidative stress was induced with H2O2.In addition,JuA pre-treatment enhanced the inhibitory effect of hUC-MSCs against abnormally activated PBMCs,which was related to stimulation of the expression and activity of indoleamine 2,3-dioxygenase.In conclusion,our results demonstrate that JuA pre-treatment can enhance the survival and immunomodulatory ability through pathways related to oxidative stress,providing a new option for the improvement of hUC-MSCs in the clinical setting.

Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic

The extract from Semen Ziziphi Spinosae(SZS)contains many secondary metabolites(flavonoids,alkaloids,and terpenoids),widely reported for their high medicinal value.In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS,we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic.A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages(T1,T2,and T3)of SZS.The main flavonoid metabolic components of SZS were catechin,L-epicatechin,(–)-epigallocatechin,(+)-gallocatechin,spinosin and its derivatives.A total of 53 unigenes encoding 15 enzymes were identified as candidate genes involved in flavonoid biosynthesis in SZS.Among,PAL,C4H,4CL,CHS,CHI,FLS,FNS,ANS,ANR,LAR,and UFGT genes were all downregulated in T3 than in T1.The expression levels of these genes influence the accumulation of flavonoids in SZS.Our results should provide valuable information for flavonoid metabolites and candidate genes involved in flavonoid biosynthesis on SZS .

cAMP/Epac/Rap1信号通路调控NG2细胞分泌IL-1β、TNF-α、BDNF及酸枣仁皂苷A的作用研究

目的研究环磷酸腺苷(cAMP)/环磷酸腺苷结合蛋白(Epac)/Ras相关蛋白1(Rap1)信号通路是否参与调控NG2细胞分泌白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、脑源性神经营养因子(BDNF)及酸枣仁皂苷A(JuA)的作用。方法体外培养NG2细胞,分为对照组、百日咳毒素(PTX)组、非环状核苷酸Epac拮抗剂(ESI-09)组、酸枣仁皂苷A(JuA)组、艾司唑仑(阳性药)组。采用CCK-8法检测不同浓度JuA对NG2细胞存活率的影响,RT-PCR法和Western blot法检测各组细胞IL-1β、TNF-α、BDNF、cAMP、Epac、Rap1 mRNA及蛋白的表达情况。结果与对照组比较,PTX组降低IL-1β和TNF-αmRNA和蛋白的表达(P<0.01),增加BDNF和cAMP mRNA和蛋白的表达(P<0.01);ESI-09组增加IL-1β和TNF-αmRNA和蛋白的表达(P<0.05),降低BDNF、Epac和Rap1 mRNA和蛋白的表达(P<0.01);JuA组、阳性药组均增加IL-1β、TNF-α、BDNF、cAMP、Epac、Rap1 mRNA和蛋白的表达(P<0.05)。结论cAMP/Epac/Rap1信号通路可介导NG2细胞分泌IL-1β、TNF-α、BDNF,JuA可能作用于cAMP/Epac/Rap1信号通路影响NG2细胞分泌BDNF。

正交设计优选枣仁安神酒的回流提取工艺

目的筛选回流法制备枣仁安神酒的最佳提取工艺。方法以芒果苷、阿魏酸、甘草苷、斯皮诺素、甘草酸铵5种指标性成分的含量和总固体含量为指标,通过单因素及正交实验考察酒的度数、加酒量及回流时间对提取工艺的影响,从而优选出制备枣仁安神酒的最佳提取工艺。结果确定枣仁安神酒的最佳回流提取工艺为加10倍量的60度白酒,回流提取70 min。3批验证实验结果表明该工艺条件稳定、合理、可靠。结论经筛选出的最佳工艺制备的枣仁安神酒有效成分提取率高、稳定性好,为该制剂的深入开发提供了实验基础。

A study of storage conditions for pre-mashed fried Semen Zizyphi Spinosae using a multi-factor orthogonal design

Background:To facilitate the preparation of traditional Chinese medicines they are pre-mashed,i.e.,mashed in advance.However,storage conditions for pre-mashed traditional Chinese medicines are based on subjective judgments of pharmacists,and the best storage conditions have not generally been determined.Semen Zizyphi Spinosae is a commonly used traditional Chinese medicine,and it is usually used after it is fried.The medicine needs to be mashed in a timely manner to ensure its effectiveness.The Chinese Pharmacopoeia provides a limit for its aflatoxin content.Methods:The orthogonal experimental design method optimizes the best plan for pre-mashed fried Semen Zizyphi Spinosae.Experimental conditions were defined using the L^(9)(3^(4))orthogonal design table.Four factors and three levels were used to study storage conditions.The four factors and three levels are as follows:storage temperature(10°C,20°C,and 30°C),storage humidity(45%,60%,and 75%),storage times(10,20 and 30 days),and particle sizes for the powder(coarsest,coarse and medium powders).The contents of jujuboside A,spinosin,aflatoxin B1,total aflatoxins(aflatoxins B1,B2,G1,and G2),moisture,total ash,acid value,and saponification values were measured.Results:The results demonstrated that the highest jujuboside A and spinosin contents were obtained using a storage temperature of 20°C,a storage humidity of 75%,a storage time of 10 days,and with a coarse powder particle size.Aflatoxin B1 and total aflatoxins(aflatoxins B1,B2,G1 and G2)were not detected under these conditions.Conclusion:There is no requirement for traditional Chinese medicines to be pre-mashed.This study evaluated various storage conditions for pre-mashed fried Semen Zizyphi Spinosae,and considered the influence of four factors on the contents of jujuboside A,spinolin and aflatoxin for quality control to provide a reference for other pre-mashed traditional Chinese medicines.

酸枣仁皂苷提取物的制备与酸枣仁皂苷A和B的含量测定

目的优化酸枣仁总皂苷的大孔吸附树脂纯化工艺;并进一步建立RP-HPLC法同时测定酸枣仁皂苷提取物中酸枣仁皂苷A和B的含量。方法采用单因素方法对纯化工艺条件进行优化,得到酸枣仁皂苷提取物,进而建立RP-HPLC法对酸枣仁提取物中酸枣仁皂苷A和B进行含量测定。结果最佳纯化工艺为:采用AB-8型大孔吸附树脂进行纯化,上样吸附时间为1.5 h,洗脱流速为1.0 mL.min-1,体积分数为70%的乙醇溶液洗脱160 mL。采用此纯化方法得到的酸枣仁皂苷提取物中皂苷的质量分数为52%,测得其酸枣仁皂苷A和B的含量质量分数分别为2.298%和0.789%。酸枣仁皂苷提取物中酸枣仁皂苷A和B的质量浓度分别在14.7～293.0 mg.L-1(r=0.999 7,n=6)、7.2～144.0 mg.L-1(r=0.999 6,n=6)内与峰面积呈良好线性关系;平均回收率(n=9)分别为103.7%、101.9%。结论该工艺稳定可行且提取物中皂苷含量高,具有广泛的应用前景;建立的RP-HPLC法可为酸枣仁皂苷提取物质量标准的建立提供一定依据。

酸枣(仁、叶、肉)与酸枣皂甙A对中枢神经系统作用的实验研究

酸枣仁和酸枣叶对中枢神经系统功能有类似的抑制作用。酸枣肉与戊巴比妥纳或硫喷妥纳在延长睡眠和镇静上有协同作用和降低协调运动的作用。酸枣皂甙A对中枢神经系统功能没有抑制作用,却与苯丙胺有协同作用。

微波辅助提取酸枣仁皂苷A的工艺优化

为了优化微波辅助提取酸枣仁皂苷A的工艺,采用单因素试验和L9(34)正交试验设计,研究了提取条件对酸枣仁皂苷A产率的影响。结果表明酸枣仁皂苷A的最佳提取工艺为:5 g酸枣仁粉末用体积分数70%乙醇提取2次,提取温度60℃,提取时间8 min,液固比16∶1(mL∶g),微波功率800 W,此条件下酸枣仁皂苷A的得率为0.526 mg/g。与其它传统提取方法相比,微波辅助提取在获得较高提取得率的同时,能有效缩短提取时间,节约资源,显示了良好的应用前景。

人体粪便孵育液对酸枣仁皂苷A的代谢转化研究

目的:研究酸枣仁皂苷A在人体粪便孵育液中的代谢转化。方法:人体粪便孵育液与酸枣仁皂苷A在体外37℃厌氧条件下共同培养,通过ELSD对孵育液中酸枣仁皂苷A的含量进行检测。结果:酸枣仁皂苷A与人体粪便孵育液在体外37℃厌氧条件下共同培养24 h之后,19 min处酸枣仁皂苷A峰消失,并于22 min处出现一个新峰,经ELSD检测后鉴定代谢产物为酸枣仁皂苷B。结论:酸枣仁皂苷A在人体粪便孵育液中代谢转化为酸枣仁皂苷B。

UPLC-UV-ELSD测定不同产地炒酸枣仁饮片中酸枣仁皂苷A、酸枣仁皂苷B、斯皮诺素和6′′′-阿魏酰斯皮诺素的含量

目的建立超高效液相色谱(UPLC)-紫外(UV)-蒸发光散射检测法(ELSD)同时测定不同产地炒酸枣仁饮片中酸枣仁皂苷A、酸枣仁皂苷B、斯皮诺素和6′′′-阿魏酰斯皮诺素的含量,对不同产地炒酸枣仁饮片进行质量评价。方法采用Waters Acquity UPLC色谱系统,UV检测器,ELSD检测器,色谱柱为ACQUITY C18柱(150 mm×2.1 mm,1.7μm),流动相为0.1%甲酸水-乙腈(梯度洗脱),流速0.3 mL/min,柱温35℃,UV检测波长335 nm,进样量3μL,喷雾器参数50%,ELSD条件气压172 kPa,漂移管温度60℃。结果在上述色谱条件下测得酸枣仁皂苷A、酸枣仁皂苷B、斯皮诺素和6′′′-阿魏酰斯皮诺素分别在27.20~435.20 mg/mL、12.60~200.80 mg/mL、26.38~422.00 mg/mL、24.38~390.00 mg/mL时与色谱峰面积线性关系良好,平均回收率分别为100.01%、99.99%、100.00%、100.04%,相对标准偏差分别为0.17%、0.32%、0.32%、0.32%。结论UPLC-UV-ELSD法准确、灵敏,可作为不同产地炒酸枣仁饮片的质量控制方法,测定结果表明,河北产炒酸枣仁饮片中4种成分含量相对较高。