Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam...Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam probe, Atto565-Tet, is rationally constructed for spontaneously blinking after biorthogonal labelling and successfully applied to super resolution imaging of mitochondria and lysosomes.展开更多
A series of probes KJ-x(x=1-3)with carbon chains of different lengths based on the matrix of rhodamine B were engineered to detect Ag^(+)in aqueous solution in this work.Among them,KJ-1 is selected as the best option ...A series of probes KJ-x(x=1-3)with carbon chains of different lengths based on the matrix of rhodamine B were engineered to detect Ag^(+)in aqueous solution in this work.Among them,KJ-1 is selected as the best option after in vitro investigation in view of its most sensitive and rapid response to Ag^(+),whose possible sensing mechanism is studied by experimental investigation and theoretical calculation.To identify the practical application of the probe,the detection of Ag^(+)in nonantibiotic fungicide Silver&Health and differentiation between normal hepatocytes and hepatoma cells using confocal imaging was conducted.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21421005, 21576040, 21776037, 22004011)China Postdoctoral Science Foundation (Nos. BX20200073 and 2020M670754)Dalian Science and Technology Innovation Fund (No. 2020JJ25CY014)。
文摘Spontaneously blinking probe, which switches between dark and bright state without UV or external additives, is extremely attractive in super resolution imaging of live cells. Herein, a clickable rhodamine spirolactam probe, Atto565-Tet, is rationally constructed for spontaneously blinking after biorthogonal labelling and successfully applied to super resolution imaging of mitochondria and lysosomes.
基金the financial support from the National Natural Science Foundation of China(No.21705120)the Project of Shandong Province Higher Educational Outstanding Youth Innovation Team(No.2019KJM008)+2 种基金the Natural Science Foundation of Shandong ProvinceChina(No.ZR2017LB016)the Project of Shandong Province Higher Educational Science and Technology Program(No.J17KB074)。
文摘A series of probes KJ-x(x=1-3)with carbon chains of different lengths based on the matrix of rhodamine B were engineered to detect Ag^(+)in aqueous solution in this work.Among them,KJ-1 is selected as the best option after in vitro investigation in view of its most sensitive and rapid response to Ag^(+),whose possible sensing mechanism is studied by experimental investigation and theoretical calculation.To identify the practical application of the probe,the detection of Ag^(+)in nonantibiotic fungicide Silver&Health and differentiation between normal hepatocytes and hepatoma cells using confocal imaging was conducted.
