A new spirostanol saponin presenting strong activity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R. Kunth by bioactivity-guided fractionation. The struc...A new spirostanol saponin presenting strong activity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R. Kunth by bioactivity-guided fractionation. The structure was established as (25S)-spirost-5-en-3 beta, 27-diol-3-O- [alpha -L-rhamnopyranosyl (1 -->2)-beta -D glucopyranosyl (1 -->3)]-beta -D-glucopyranoside on the basis of chemical evidences and spectral analysis, especially by 2D-NMR techniques.展开更多
A new spirostanol sulfonate, spirost-5,25（27）-dien-1β,3β-diol 1-sulfonate（1）, was isolated from the rhizomes of Helleborus thibetanus（Ranunculaceae）, and the structure was identified on the basis of a detailed...A new spirostanol sulfonate, spirost-5,25（27）-dien-1β,3β-diol 1-sulfonate（1）, was isolated from the rhizomes of Helleborus thibetanus（Ranunculaceae）, and the structure was identified on the basis of a detailed spectroscopic analyses, including 2D NMR spectrometry, IR and HRESI-MS.展开更多
A new spirostanol steroidal saponin, named maireioside A （1）, together with three known steroidal saponins, hypoglaucin G （2）, parisaponin I （3）, and diosgenin-3-O-α-L-rhamnopyranosyl （1→4）-[α-L-rhamnopyran...A new spirostanol steroidal saponin, named maireioside A （1）, together with three known steroidal saponins, hypoglaucin G （2）, parisaponin I （3）, and diosgenin-3-O-α-L-rhamnopyranosyl （1→4）-[α-L-rhamnopyranosyl （1→2）]-β-D-glucopyranoside （4）, were isolated from the rhizomes of Paris mairei. The structure elucidation was accomplished by 1D and 2D NMR methods, HR-ESI-MS, and hydrolysis. C 2009 Qiang Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.展开更多
Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compou...Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compounds.Their structures were established mainly on the base of NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.In addition,the cytotoxicity of the two new saponins was tested.Graphical Abstract Two new highly oxygenated spirostanol saponins,paristenosides A(1)and B(2),were isolated from the rhizomes of Paris polyphylla var.stenophylla.Their structures were established mainly based on NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.展开更多
Gross saponins of Tribulus terrestris（GSTT） have exact effect on cardiovascular and cerebrovascular diseases.But as a mixture,the specific efficient component of GSTT is still unknown.Nine monomers of spirostanol sa...Gross saponins of Tribulus terrestris（GSTT） have exact effect on cardiovascular and cerebrovascular diseases.But as a mixture,the specific efficient component of GSTT is still unknown.Nine monomers of spirostanol saponins were isolated and idendified as JA―JI（named transitorily） by means of NMR spectrometry.After bio-activity screening on them,we defined that monomers tigogenin 3-O-β-D-xylopyranosyl（1→2）-[β-D-xylopyranosyl（1→3）]-β-D-glucopyranosyl（1→4）-[α-L-rhamnopyranosyl（1→2）]-β-D-galactopyranoside（compound JB） and hecogenin3-O-β-D-glucopyranosyl（1→4）-β-D-galactopyranoside（compound JG） have cytoprotective bio-activity.Compound JB display effective dose in 10-8 and 10-9 mol/L,and JG in 10-6―10-9 mol/L.Survival rate,lactate dehydrogenase（LDH） and apoptosis also show that JB（at dose 10-8,10-9 and 10-10 mol/L） can protect myocardial injury caused by hypoxia/reoxygenation（H/R）.While morphology change also shows JG has cytoprotective bio-activity.展开更多
Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L. Their structures were elucidated as (23S, 25R)-5a- spirostan-3b, 6a, 11a, 23-tetraol (1) and (23S,...Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L. Their structures were elucidated as (23S, 25R)-5a- spirostan-3b, 6a, 11a, 23-tetraol (1) and (23S, 25S)-5a-spirostan-3b, 23, 27-triol (2) by spectral methods.展开更多
A facile glycosylation for the synthesis of spirostanol glycosides was reported. Using cadmium carbonate as a catalyst in CH3CN, a series of saponins was synthesized efficiently in a satisfactory yield.
Two minor steroidal saponins were isolated from the roots of Smilax menispermoidea.One of them is new and its structure was established as(25S) spirost-5-en-3β,17α,27-triol-3-O-[α-L-rhamnopyranosyl(1-4)]-β-D-gluco...Two minor steroidal saponins were isolated from the roots of Smilax menispermoidea.One of them is new and its structure was established as(25S) spirost-5-en-3β,17α,27-triol-3-O-[α-L-rhamnopyranosyl(1-4)]-β-D-gluco- pyranoside using spectroscopic and chemical methods.展开更多
文摘A new spirostanol saponin presenting strong activity of inducing morphological deformation of Pyricularia oryzae mycelia was isolated from Dioscorea futshauensis R. Kunth by bioactivity-guided fractionation. The structure was established as (25S)-spirost-5-en-3 beta, 27-diol-3-O- [alpha -L-rhamnopyranosyl (1 -->2)-beta -D glucopyranosyl (1 -->3)]-beta -D-glucopyranoside on the basis of chemical evidences and spectral analysis, especially by 2D-NMR techniques.
基金Supported by the Program for New Century Excellent Talents in University, China(No.NCET-09-0589) China Postdoc-toral Science Foundation(No. 20090450775)
文摘A new spirostanol sulfonate, spirost-5,25（27）-dien-1β,3β-diol 1-sulfonate（1）, was isolated from the rhizomes of Helleborus thibetanus（Ranunculaceae）, and the structure was identified on the basis of a detailed spectroscopic analyses, including 2D NMR spectrometry, IR and HRESI-MS.
文摘A new spirostanol steroidal saponin, named maireioside A （1）, together with three known steroidal saponins, hypoglaucin G （2）, parisaponin I （3）, and diosgenin-3-O-α-L-rhamnopyranosyl （1→4）-[α-L-rhamnopyranosyl （1→2）]-β-D-glucopyranoside （4）, were isolated from the rhizomes of Paris mairei. The structure elucidation was accomplished by 1D and 2D NMR methods, HR-ESI-MS, and hydrolysis. C 2009 Qiang Wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
基金This work is funded by the National Natural Science Funding of China(Grand No.31570363)the Key Research Program of the Chinese Academy of Sciences(Grand No.KSZDEW-Z-004-03-4)the Natural Science Foundation of Yunnan Province(Grand No.2015FA031).
文摘Phytochemical investigation of the rhizomes of Paris polyphylla var.stenophylla led to the isolation of two new highly oxygenated spirostanol saponins,named paristenosides A(1)and B(2),together with seven known compounds.Their structures were established mainly on the base of NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.In addition,the cytotoxicity of the two new saponins was tested.Graphical Abstract Two new highly oxygenated spirostanol saponins,paristenosides A(1)and B(2),were isolated from the rhizomes of Paris polyphylla var.stenophylla.Their structures were established mainly based on NMR spectroscopic techniques and mass spectrometry,as well as chemical methods.
基金Supported by the National Natural Science Foundation of China(Nos.30472020 and 30672654)
文摘Gross saponins of Tribulus terrestris（GSTT） have exact effect on cardiovascular and cerebrovascular diseases.But as a mixture,the specific efficient component of GSTT is still unknown.Nine monomers of spirostanol saponins were isolated and idendified as JA―JI（named transitorily） by means of NMR spectrometry.After bio-activity screening on them,we defined that monomers tigogenin 3-O-β-D-xylopyranosyl（1→2）-[β-D-xylopyranosyl（1→3）]-β-D-glucopyranosyl（1→4）-[α-L-rhamnopyranosyl（1→2）]-β-D-galactopyranoside（compound JB） and hecogenin3-O-β-D-glucopyranosyl（1→4）-β-D-galactopyranoside（compound JG） have cytoprotective bio-activity.Compound JB display effective dose in 10-8 and 10-9 mol/L,and JG in 10-6―10-9 mol/L.Survival rate,lactate dehydrogenase（LDH） and apoptosis also show that JB（at dose 10-8,10-9 and 10-10 mol/L） can protect myocardial injury caused by hypoxia/reoxygenation（H/R）.While morphology change also shows JG has cytoprotective bio-activity.
文摘Two new spirostanol sapogenins named agavegenin A and B were isolated from the fermented leaves of Agave americana L. Their structures were elucidated as (23S, 25R)-5a- spirostan-3b, 6a, 11a, 23-tetraol (1) and (23S, 25S)-5a-spirostan-3b, 23, 27-triol (2) by spectral methods.
文摘A facile glycosylation for the synthesis of spirostanol glycosides was reported. Using cadmium carbonate as a catalyst in CH3CN, a series of saponins was synthesized efficiently in a satisfactory yield.
基金Project supported by the National Natural Scicnce Foundation of Chinathe grant for doctoral program from State Education Commission of China
文摘Two minor steroidal saponins were isolated from the roots of Smilax menispermoidea.One of them is new and its structure was established as(25S) spirost-5-en-3β,17α,27-triol-3-O-[α-L-rhamnopyranosyl(1-4)]-β-D-gluco- pyranoside using spectroscopic and chemical methods.
