With the continual growth of the variety and complexity of network crime means, the traditional packet feature matching cannot detect all kinds of intrusion behaviors completely. It is urgent to reassemble network str...With the continual growth of the variety and complexity of network crime means, the traditional packet feature matching cannot detect all kinds of intrusion behaviors completely. It is urgent to reassemble network stream to perform packet processing at a semantic level above the network layer. This paper presents an efficient TCP stream reassembly mechanism for real-time processing of high-speed network traffic. By analyzing the characteristics of network stream in high-speed network and TCP connection establishment process, several polices for designing the reassembly mechanism are built. Then, the reassembly implementation is elaborated in accordance with the policies. Finally, the reassembly mechanism is compared with the traditional reassembly mechanism by the network traffic captured in a typical gigabit gateway. Experiment results illustrate that the reassembly mechanism is efficient and can satisfy the real-time property requirement of traffic analysis system in high-speed network.展开更多
Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a d...Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a dense network of cytoskeletal proteins,cell adhesion molecules,and voltage gated ion channels and is located at the proximal most region of the axon（Koleand Stuart, 2012）.展开更多
Tens of thousands of protein-protein interactions (PPIs) have been found in human cells and many of these macromolecular partnerships could determine the cell growth and death. Thus there is a need to develop the meth...Tens of thousands of protein-protein interactions (PPIs) have been found in human cells and many of these macromolecular partnerships could determine the cell growth and death. Thus there is a need to develop the methods to catalogue these macromolecules by detecting their interactions, modifications, and cellular locations. It will be helpful for scientists to compare the difference between a diseased cellular state and its normal state and to find the potential therapy treatment to intervene this status. One technology called split-protein reassembly or protein fragment complementation has been developed in the last two decades. This technology makes use of appropriate fragmentation of some protein reporters and the refolding of these reports could be detected by their function to confirm the interaction of interest. This system has been set up in cell-free systems, </span><i><span style="font-family:Verdana;">E.</span></i></span><i><span style="font-family:""> </span></i><i><span style="font-family:Verdana;">coli</span></i><span style="font-family:""><span style="font-family:Verdana;">, yeast, mammalian cells, plants and live animals. Herein, I present the development in fluorescence- and bioluminescence-based split-protein biosensors in both binary and ternary systems. In addition, some people developed the split-protein system by combining it with chemical inducer of dimerization strategy (CID). This has been applied for identifying the enzyme inhibitors and regulating the activity of protein kinases and phosphatases. With effort from many laboratories from the world, a variety of split-protein systems have been developed for studying the PPI </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;">, monitoring the biological process, and controlling the activity of the enzyme of interest.展开更多
Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembl...Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembled intoliposome bilayer, and the liposomes were fused into giant liposomes with a diameter more than 10μm by dehydration-rehydration procedure. The giant liposomes were used to study the Cl^- channel activities by patch-clamp technique.Results: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100//pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 ± 8.3) pS,(117.0±5.7) pS and (144.7±4.5) pS, respectively, were detected. Their activities were voltage-dependent and allwere blocked by SITS (4-acetamido-4'-isothiocyanato-stilbene-2', 2'-disulfonic acid) in a concentration-dependentmanner. By constructing the open and close dwell time distribution histograms and then fitting them with exponentialfunction, two time constants were obtained in both the open and the close states. The burst activity and conductancesubstate of the channels were observed. Conclusion; There exist three kinds of Cl^- channels with different conduc-tance in human sperm membrane at least. (Asian J Androl 2001 Sep; 3: 185 - 191)展开更多
战斗部破片群运动参数对弹药毁伤威力评估具有重要的意义。针对破片尺寸较小、背景信息复杂以及破片数据样本少导致的破片检测精度较低的问题,本文提出一种YOLOv5-FD的战斗部破片群目标检测方法。首先,在网络输出端增加微小目标检测层,...战斗部破片群运动参数对弹药毁伤威力评估具有重要的意义。针对破片尺寸较小、背景信息复杂以及破片数据样本少导致的破片检测精度较低的问题,本文提出一种YOLOv5-FD的战斗部破片群目标检测方法。首先,在网络输出端增加微小目标检测层,将原始的三尺度改为四尺度,并在特征融合网络中引入内容感知特征重组(Content Aware ReAssembly of Features,CARAFE)上采样模块替换原有的最近邻插值上采样,减少小目标特征信息损失,提高弱小破片的提取能力。其次,在特征提取网络引入坐标注意力模块(Coordinate Attention,CA),加强对破片特征的提取,弱化背景信息,抑制复杂背景的干扰。最后,在模型训练过程中引入模型不可知元学习方法(Model Agnostic Meta Learning,MAML),达到仅用小样本破片数据集实现较高的检测性能。实验结果表明,YOLOv5-FD破片检测算法在自制破片数据集中,精确率达到了90.5%,召回率达到了85.4%,平均精度mAP_0.5达到了88.2%,与原始YOLOv5s算法相比分别提高了7.1%,7.9%和7.5%,有效提高了破片目标检测准确性。展开更多
隧道已经在互联网中大量部署。隧道通信中两次端到端通信及途中封装及解封装的特点,会导致隧道出口出现重组现象。分析了隧道中重组的具体代价,表明重组极大地影响着隧道转发性能。对现有隧道MTU问题解决方案进行了研究,提出了在隧道入...隧道已经在互联网中大量部署。隧道通信中两次端到端通信及途中封装及解封装的特点,会导致隧道出口出现重组现象。分析了隧道中重组的具体代价,表明重组极大地影响着隧道转发性能。对现有隧道MTU问题解决方案进行了研究,提出了在隧道入口实施的SFCT(SRC Fragment Completely for Tunnel)模型和FATE(Fragment in Advance on Tunnel Entry)模型来规避隧道中的重组,并给出了主要类型隧道中MTU问题的方案选择策略。模拟实验验证了其可用性及较好的性能。展开更多
基金National High-Tech Research and Development Program of China (863 Program) (No.2007AA01Z309)
文摘With the continual growth of the variety and complexity of network crime means, the traditional packet feature matching cannot detect all kinds of intrusion behaviors completely. It is urgent to reassemble network stream to perform packet processing at a semantic level above the network layer. This paper presents an efficient TCP stream reassembly mechanism for real-time processing of high-speed network traffic. By analyzing the characteristics of network stream in high-speed network and TCP connection establishment process, several polices for designing the reassembly mechanism are built. Then, the reassembly implementation is elaborated in accordance with the policies. Finally, the reassembly mechanism is compared with the traditional reassembly mechanism by the network traffic captured in a typical gigabit gateway. Experiment results illustrate that the reassembly mechanism is efficient and can satisfy the real-time property requirement of traffic analysis system in high-speed network.
基金supported by National Institutes of Health Grants NS069688 and NS044916, TIRR Foundationthe Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
文摘Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a dense network of cytoskeletal proteins,cell adhesion molecules,and voltage gated ion channels and is located at the proximal most region of the axon（Koleand Stuart, 2012）.
文摘Tens of thousands of protein-protein interactions (PPIs) have been found in human cells and many of these macromolecular partnerships could determine the cell growth and death. Thus there is a need to develop the methods to catalogue these macromolecules by detecting their interactions, modifications, and cellular locations. It will be helpful for scientists to compare the difference between a diseased cellular state and its normal state and to find the potential therapy treatment to intervene this status. One technology called split-protein reassembly or protein fragment complementation has been developed in the last two decades. This technology makes use of appropriate fragmentation of some protein reporters and the refolding of these reports could be detected by their function to confirm the interaction of interest. This system has been set up in cell-free systems, </span><i><span style="font-family:Verdana;">E.</span></i></span><i><span style="font-family:""> </span></i><i><span style="font-family:Verdana;">coli</span></i><span style="font-family:""><span style="font-family:Verdana;">, yeast, mammalian cells, plants and live animals. Herein, I present the development in fluorescence- and bioluminescence-based split-protein biosensors in both binary and ternary systems. In addition, some people developed the split-protein system by combining it with chemical inducer of dimerization strategy (CID). This has been applied for identifying the enzyme inhibitors and regulating the activity of protein kinases and phosphatases. With effort from many laboratories from the world, a variety of split-protein systems have been developed for studying the PPI </span><i><span style="font-family:Verdana;">in vitro</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">in vivo</span></i><span style="font-family:Verdana;">, monitoring the biological process, and controlling the activity of the enzyme of interest.
文摘Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembled intoliposome bilayer, and the liposomes were fused into giant liposomes with a diameter more than 10μm by dehydration-rehydration procedure. The giant liposomes were used to study the Cl^- channel activities by patch-clamp technique.Results: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100//pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 ± 8.3) pS,(117.0±5.7) pS and (144.7±4.5) pS, respectively, were detected. Their activities were voltage-dependent and allwere blocked by SITS (4-acetamido-4'-isothiocyanato-stilbene-2', 2'-disulfonic acid) in a concentration-dependentmanner. By constructing the open and close dwell time distribution histograms and then fitting them with exponentialfunction, two time constants were obtained in both the open and the close states. The burst activity and conductancesubstate of the channels were observed. Conclusion; There exist three kinds of Cl^- channels with different conduc-tance in human sperm membrane at least. (Asian J Androl 2001 Sep; 3: 185 - 191)
文摘战斗部破片群运动参数对弹药毁伤威力评估具有重要的意义。针对破片尺寸较小、背景信息复杂以及破片数据样本少导致的破片检测精度较低的问题,本文提出一种YOLOv5-FD的战斗部破片群目标检测方法。首先,在网络输出端增加微小目标检测层,将原始的三尺度改为四尺度,并在特征融合网络中引入内容感知特征重组(Content Aware ReAssembly of Features,CARAFE)上采样模块替换原有的最近邻插值上采样,减少小目标特征信息损失,提高弱小破片的提取能力。其次,在特征提取网络引入坐标注意力模块(Coordinate Attention,CA),加强对破片特征的提取,弱化背景信息,抑制复杂背景的干扰。最后,在模型训练过程中引入模型不可知元学习方法(Model Agnostic Meta Learning,MAML),达到仅用小样本破片数据集实现较高的检测性能。实验结果表明,YOLOv5-FD破片检测算法在自制破片数据集中,精确率达到了90.5%,召回率达到了85.4%,平均精度mAP_0.5达到了88.2%,与原始YOLOv5s算法相比分别提高了7.1%,7.9%和7.5%,有效提高了破片目标检测准确性。
文摘隧道已经在互联网中大量部署。隧道通信中两次端到端通信及途中封装及解封装的特点,会导致隧道出口出现重组现象。分析了隧道中重组的具体代价,表明重组极大地影响着隧道转发性能。对现有隧道MTU问题解决方案进行了研究,提出了在隧道入口实施的SFCT(SRC Fragment Completely for Tunnel)模型和FATE(Fragment in Advance on Tunnel Entry)模型来规避隧道中的重组,并给出了主要类型隧道中MTU问题的方案选择策略。模拟实验验证了其可用性及较好的性能。
