Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana indust...Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry. In order to reveal the infection process and pathogenesis of Foc TR4, the young mycelia(66.7 mg/ml) of wild-type strain of Foc TR4(WT-Foc TR4) cultured for 18-20 h were lysed with enzyme mixture for protoplast formation, which consisted of 25 mg/ml driselase, 0.4 mg/ml chitinase, 15 mg/ml lysing enzyme and 1.2 mol/L potassium chloride. The resulted protoplasts of 2×10~7 cells/ml were used to test the efficiency of transformation mediated by polyethylene glycol, and up to 9 transformants per microgram of DNA were obtained. AmCyan, RFP and YFP genes were stably transferred into the WT-Foc TR4, separately, using the protoplast transformation system. The gene FoOCH1 encoding α-1, 6-mannosyltransferase in the WT-Foc TR4 was knocked out using the split-marker recombination technology. The genetic transformation and gene knockout system in this pathogen lays a foundation for the study of functional genomics and plant-pathogen interactions.展开更多
在Government and Binding Theory框架下讨论汉语中表示强调的“是”字句的词性及其所处位置.在树形图的描述上,运用LuigiRizzi关于splitComp假说中的理论,印证了“是”是焦点标记词的观点;同时,根据splitComp假说发展“是”是焦点标记...在Government and Binding Theory框架下讨论汉语中表示强调的“是”字句的词性及其所处位置.在树形图的描述上,运用LuigiRizzi关于splitComp假说中的理论,印证了“是”是焦点标记词的观点;同时,根据splitComp假说发展“是”是焦点标记词的观点,认为“是”是FocusP的中心语,位于“是”之前的成分是从TP生成然后显性移位到TopP位置上,而被强调成分隐性移位到[Spec,FocusP]的位置获得解释.展开更多
基金National Natural Science Foundation of China(30840014)SRF for ROCS,SEM(Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry)Natural Science Foundation of Shandong Province,China(ZR2010CM011)~~
基金Supported by Yunnan Science and Technology Innovation Talent Program(2015HA034)National Natural Science Foundation of China(NSFC31560505)
文摘Fusarium wilt of banana, caused by Fusarium oxysporum f. sp. cubense tropic race 4(Foc TR4), is a typical vascular and soil-borne disease which has significantly threatened the sustainable development of banana industry. In order to reveal the infection process and pathogenesis of Foc TR4, the young mycelia(66.7 mg/ml) of wild-type strain of Foc TR4(WT-Foc TR4) cultured for 18-20 h were lysed with enzyme mixture for protoplast formation, which consisted of 25 mg/ml driselase, 0.4 mg/ml chitinase, 15 mg/ml lysing enzyme and 1.2 mol/L potassium chloride. The resulted protoplasts of 2×10~7 cells/ml were used to test the efficiency of transformation mediated by polyethylene glycol, and up to 9 transformants per microgram of DNA were obtained. AmCyan, RFP and YFP genes were stably transferred into the WT-Foc TR4, separately, using the protoplast transformation system. The gene FoOCH1 encoding α-1, 6-mannosyltransferase in the WT-Foc TR4 was knocked out using the split-marker recombination technology. The genetic transformation and gene knockout system in this pathogen lays a foundation for the study of functional genomics and plant-pathogen interactions.
文摘在Government and Binding Theory框架下讨论汉语中表示强调的“是”字句的词性及其所处位置.在树形图的描述上,运用LuigiRizzi关于splitComp假说中的理论,印证了“是”是焦点标记词的观点;同时,根据splitComp假说发展“是”是焦点标记词的观点,认为“是”是FocusP的中心语,位于“是”之前的成分是从TP生成然后显性移位到TopP位置上,而被强调成分隐性移位到[Spec,FocusP]的位置获得解释.
