ATP-binding cassette transporter C2(ABCC2)is known to be a receptor for Bacillus thuringiensis(Bt)toxins in several lepidopteran insects.Mutations in the ABCC2 gene have been genetically linked to field-evolved resist...ATP-binding cassette transporter C2(ABCC2)is known to be a receptor for Bacillus thuringiensis(Bt)toxins in several lepidopteran insects.Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1 F toxin from Bt in Spodoptera frugiperda.Here we generated a SfABCC2 knockout strain of S.frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1 F.Results from bioassays showed that the SfABCC2 knockout S.frugiperda strain displayed 118-fold resistance to Cry1 F compared with the parental DH19 strain,but no resistance to Vip3 A toxin from Bt.These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1 F.展开更多
Odorant receptor(OR)is crucial for insects to detect and recognize external chemical cues closely related to their survival.The insect OR forms a heteromeric complex composed of a ligand-specific receptor and a ubiqui...Odorant receptor(OR)is crucial for insects to detect and recognize external chemical cues closely related to their survival.The insect OR forms a heteromeric complex composed of a ligand-specific receptor and a ubiquitously odorant receptor coreceptor(Orco).This study used the CRISPR/Cas9 technique to knock out(KO)Orco and reveal its essential role in acting on OR-meditated olfactory behavior in a critical invasive agricultural pest,the fall armyworm(FAW),Spodoptera frugiperda.Electroantennogram(EAG)results suggested that the Orco mutants of both male and female moths severely reduced their electrophysiological responses to the eight tested plant volatiles and two sex pheromones.However,the Orco gene played distinct roles in mating behavior between sexes:the mating behavior was fully disrupted in mutated males but not in mutated females.The oviposition result indicated that the Orco KO females displayed reduced egg laying by 24.1%compared with the mated wild type(WT)females.Overall,these results strongly suggest that Orco is an excellent target for disrupting FAW’s normal behavior and provides a feasible pest control approach.展开更多
基金supported by the Key Project for Breeding Genetic Modified Organisms of China(2016ZX08012004003)。
文摘ATP-binding cassette transporter C2(ABCC2)is known to be a receptor for Bacillus thuringiensis(Bt)toxins in several lepidopteran insects.Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1 F toxin from Bt in Spodoptera frugiperda.Here we generated a SfABCC2 knockout strain of S.frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1 F.Results from bioassays showed that the SfABCC2 knockout S.frugiperda strain displayed 118-fold resistance to Cry1 F compared with the parental DH19 strain,but no resistance to Vip3 A toxin from Bt.These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1 F.
基金the National Natural Science Foundation of China(32130089)the China Postdoctoral Science Foundation(2020M680785)+3 种基金the Shenzhen Science and Technology Program,China(KQTD20180411143628272)the Central Public-interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202108)the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District,China(PT202101-02)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP).
文摘Odorant receptor(OR)is crucial for insects to detect and recognize external chemical cues closely related to their survival.The insect OR forms a heteromeric complex composed of a ligand-specific receptor and a ubiquitously odorant receptor coreceptor(Orco).This study used the CRISPR/Cas9 technique to knock out(KO)Orco and reveal its essential role in acting on OR-meditated olfactory behavior in a critical invasive agricultural pest,the fall armyworm(FAW),Spodoptera frugiperda.Electroantennogram(EAG)results suggested that the Orco mutants of both male and female moths severely reduced their electrophysiological responses to the eight tested plant volatiles and two sex pheromones.However,the Orco gene played distinct roles in mating behavior between sexes:the mating behavior was fully disrupted in mutated males but not in mutated females.The oviposition result indicated that the Orco KO females displayed reduced egg laying by 24.1%compared with the mated wild type(WT)females.Overall,these results strongly suggest that Orco is an excellent target for disrupting FAW’s normal behavior and provides a feasible pest control approach.
