Sporotrichosis is a subcutaneous mycosis caused by the Sporothrix schenckii complex. It has three classic clinical variants: fixed, lymphangitic, and systemic. Treatment in most cases has been itraconazole or potassiu...Sporotrichosis is a subcutaneous mycosis caused by the Sporothrix schenckii complex. It has three classic clinical variants: fixed, lymphangitic, and systemic. Treatment in most cases has been itraconazole or potassium iodide. The aim of this paper is to communicate an unusual relapsing case treated with IK. We report a 73-year-old woman with lymphangitic sporotrichosis, adequate response to treatment with potassium iodide, and recurrence 15 months after. A molecular test was performed through the amplification of a 331 bp fragment of the calmodulin gene. In both infections, the same specie was isolated. The effects of potassium iodide are briefly discussed, and we conclude that the same treatment can be prescribed, if no side effects are observed.展开更多
Sporothrix schenckii （S. schenckii）, a dimorphic fungus, is the etiological agent of sporotrichosis. After entrance of microconidia or mycelial fragments into a mammalian host, the fungus differentiates into the par...Sporothrix schenckii （S. schenckii）, a dimorphic fungus, is the etiological agent of sporotrichosis. After entrance of microconidia or mycelial fragments into a mammalian host, the fungus differentiates into the parasitic yeast form. Meanwhile, several defensive signals would be triggered when the innate immune system was stimulated by S. schenckii invasion and microbe-specific phagocytosis. The success of S. schenckii infection partly depends on its ability to avoid oxidative damage from reactive oxygen species （ROS） released by polymorphonuclear leukocytes and activated macrophages. However, the antioxidant defense mechanisms of S. schenckii remains unknown. Catalases, one of the central enzymes involved in scavenging ROS via converting hydrogen peroxide （H2O2） to water and molecular oxygen, play an essential role in protecting intracellular pathogenic fungi against ROS and regulating growth and development in some fungi. No catalase in S. schenckii has been identified and characterized previously. Recently we have cloned a catalase homologous gene from the yeast form of S. schenckii and designated it Sscat. In this report,展开更多
s To investigate the DNA polymorphism of Sporothrix schenckii ( S schencki i ) and to find the relationship between DNA patterns and geographic areas and clinical manifestations Method The total DNA was ...s To investigate the DNA polymorphism of Sporothrix schenckii ( S schencki i ) and to find the relationship between DNA patterns and geographic areas and clinical manifestations Method The total DNA was extracted with hexadecyltrimethyl ammonium bromide Random A mplification of Polymorphic DNA (RAPD) assay was used to study DNA typing of 24 strains of S schenckii collected from different areas and isolated from di fferent clinical types Results Of seven random primers used, three primers (OPAA11, OPD18 and OPB07) gave good reactions, the sequences of which were 5' ACCCGACCTG 3', 5' GAGAGCCAAC 3', 5 ' GGTGAC^GCAG 3' respectively The RAPD patterns of the 24 isolates were not completely identical, showing certain degrees of hereditary variability Differ ent isolates showed a common conserved DNA band with the same primer Different clinical types showed different genotypes Conclusion RAPD analysis is useful in DNA typing of S schenckii , the DNA band type of which is related to geographic origin and Clinical manifestation展开更多
Molecular phylogeny has revealed that sporotrichosis is caused by several Sporothrix species which differ in clinical behavior.The complex is embedded within Ophiostoma,a genus mainly comprising fungi that live in ass...Molecular phylogeny has revealed that sporotrichosis is caused by several Sporothrix species which differ in clinical behavior.The complex is embedded within Ophiostoma,a genus mainly comprising fungi that live in association with bark beetles,but differs by a high virulence towards humans and other mammals.The different ecology is corroborated by phylogenetic separation.The aim of the present study was to determine the validity of the rDNA internal transcribed spacer(ITS)region as a marker for diagnostics of species in the clinical group,using betatubulin sequences to calibrate species delimitations.The topology of the two gene trees was concordant,and all clinically relevant Sporothrix species could easily be recognized by means of the ITS region.An increased geographic sampling did not affected delimitation success in the clinical clade of the S.schenckii complex.展开更多
文摘Sporotrichosis is a subcutaneous mycosis caused by the Sporothrix schenckii complex. It has three classic clinical variants: fixed, lymphangitic, and systemic. Treatment in most cases has been itraconazole or potassium iodide. The aim of this paper is to communicate an unusual relapsing case treated with IK. We report a 73-year-old woman with lymphangitic sporotrichosis, adequate response to treatment with potassium iodide, and recurrence 15 months after. A molecular test was performed through the amplification of a 331 bp fragment of the calmodulin gene. In both infections, the same specie was isolated. The effects of potassium iodide are briefly discussed, and we conclude that the same treatment can be prescribed, if no side effects are observed.
文摘Sporothrix schenckii （S. schenckii）, a dimorphic fungus, is the etiological agent of sporotrichosis. After entrance of microconidia or mycelial fragments into a mammalian host, the fungus differentiates into the parasitic yeast form. Meanwhile, several defensive signals would be triggered when the innate immune system was stimulated by S. schenckii invasion and microbe-specific phagocytosis. The success of S. schenckii infection partly depends on its ability to avoid oxidative damage from reactive oxygen species （ROS） released by polymorphonuclear leukocytes and activated macrophages. However, the antioxidant defense mechanisms of S. schenckii remains unknown. Catalases, one of the central enzymes involved in scavenging ROS via converting hydrogen peroxide （H2O2） to water and molecular oxygen, play an essential role in protecting intracellular pathogenic fungi against ROS and regulating growth and development in some fungi. No catalase in S. schenckii has been identified and characterized previously. Recently we have cloned a catalase homologous gene from the yeast form of S. schenckii and designated it Sscat. In this report,
基金ThisworkwasfinanciallysupportedbyagrantfromtheEducationCommissionofLiaoningProvince (No 990 2 2 1 0 69)
文摘s To investigate the DNA polymorphism of Sporothrix schenckii ( S schencki i ) and to find the relationship between DNA patterns and geographic areas and clinical manifestations Method The total DNA was extracted with hexadecyltrimethyl ammonium bromide Random A mplification of Polymorphic DNA (RAPD) assay was used to study DNA typing of 24 strains of S schenckii collected from different areas and isolated from di fferent clinical types Results Of seven random primers used, three primers (OPAA11, OPD18 and OPB07) gave good reactions, the sequences of which were 5' ACCCGACCTG 3', 5' GAGAGCCAAC 3', 5 ' GGTGAC^GCAG 3' respectively The RAPD patterns of the 24 isolates were not completely identical, showing certain degrees of hereditary variability Differ ent isolates showed a common conserved DNA band with the same primer Different clinical types showed different genotypes Conclusion RAPD analysis is useful in DNA typing of S schenckii , the DNA band type of which is related to geographic origin and Clinical manifestation
基金Xun Zhou acknowledge financial support from China NSFC 31270062Chongqing Science and Technology Commission cstc2011jjA10089+1 种基金Anderson Rodrigues is a fellow and acknowledges the financial support of the Fundação de AmparoàPesquisa do Estado de São Paulo(FAPESP-2011/07350-1)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(BEX 2325/11-0)。
文摘Molecular phylogeny has revealed that sporotrichosis is caused by several Sporothrix species which differ in clinical behavior.The complex is embedded within Ophiostoma,a genus mainly comprising fungi that live in association with bark beetles,but differs by a high virulence towards humans and other mammals.The different ecology is corroborated by phylogenetic separation.The aim of the present study was to determine the validity of the rDNA internal transcribed spacer(ITS)region as a marker for diagnostics of species in the clinical group,using betatubulin sequences to calibrate species delimitations.The topology of the two gene trees was concordant,and all clinically relevant Sporothrix species could easily be recognized by means of the ITS region.An increased geographic sampling did not affected delimitation success in the clinical clade of the S.schenckii complex.
基金Project supported by the National Natural Science Foundation of China （No. 31270062） and the Chongqing Science and Technology Commission （No. cstc2011jjA10089）, China
