The Effects of Nutritional Media and Initial Cell Density on the Growth and Development of Spirulina platensis

The main objective of the study was to determine the effects of nutritional media and initial cell density on the growth and development of Spirulina platensis.This study was carried out at the Center for Incubation and Technology Transfer,Hue University from May 2015 to August 2016.In the first experiment,S.platensis was cultured in four different nutritional media,including Zarrouk,Spi-RIA3,Nisole and Thuoc medium(Vietnamese).Resulted showed that S.platensis grew significantly in the Zarrouk medium rather than in other nutritional medium at the same time,and gained the maximum density of 2,736.5 mg/L after 13 d inoculums,with specific growth rate of 4.987%per day and the time to doubling population of 0.139 d.In the second experiment,S.platensis was cultured in six different initial cell densities:100,400,700,1,000,1,300 and 1,600 mg/L,and the optimum nutritional medium was Zarrouk medium selected from experiment one.Results showed at the initial culture density of 400 mg/L,the highest biomass was gained with the maximum density of 3,071.2 mg/L after 21 d inoculums,with specific growth rate of 9.15%per day and time to double population of 0.076 d.S.platensis biomass from culture in Zarrouk medium at initial cell density 400 mg/L obtained total protein of 51.98%,total lipid of 1.75%,total carbohydrate of 0.75%,carotene of 0.36%and total minerals of 5.2%,on a basis of dry matter.

6种植物生长调节剂对钝顶螺旋藻生长的影响

研究6种植物生长调节剂对钝顶螺旋藻(SpirulinaplatensisA9)生长的影响。结果表明,0 5mg·L-1的GA、1 0mg·L-1的6 BA、5mg·L-1的NAA和0 5mg·L-1的2,4 D对螺旋藻的生长具有明显的促进作用,特别是在培养的前6d。在交叉试验过程中,生长素NAA和细胞分裂素6 BA结合使用效果相对较好。螺旋藻内源激素的测定结果进一步为激素促进螺旋藻的生长提供了理论依据。

铅污染对钝顶螺旋藻生长及某些生理性状的影响

通过在Ｚａｒｒｏｕｋ培养基中加入不同浓度的Ｐｂ^（２＋）研究水环境污染对钝顶螺旋藻（Ｓｐｉｒｉｕｌｉｎａ ｐｌａｔｅｎｓｉｓ）生长、藻蓝蛋白和光合作用、膜脂过氧化反应及以超氧物歧化酶（ＳＯＤ）为代表的自由基清除系统的影响发现当 Ｐｂ^（２＋）浓度高于 ２０ｍｇ·Ｌ^（－１）时，钝顶螺旋藻生长受抑制，藻细胞随着浓度的升高和时间的延长而黄化死亡．细胞可溶性蛋白含量、藻蓝蛋白含量、超氧物歧化酶（ＳＯＤ）活性均随着Ｐｂ^（２＋）浓度增加而逐渐下降。是极显著的负相关．低浓度Ｐｂ^（２＋）使光合速率大幅度下降但完整细胞的吸收光谱在高浓度时才发生急剧变化．Ｐｂ^（２＋）处理使超氧阴离子^（Ｏ２－）和丙二醛（ ＭＤＡ）含量上升，其峰值分别出现在 ３０ｍｇ· Ｌ^（－１）和 ４０ｍｇ· Ｌ^（－１）处理组． ＳＯＤ活性在 ２０ｍｇ· Ｌ^（－１）以上显著下降，但其比活性在２０－５０ｍｇ·Ｌ^（－１）范围内仍维持较高水平，且相对稳定．

钝顶螺旋藻处理氨氮废水的研究

螺旋藻是一种营养丰富的新型保健食品,利用含氮废水为培养基,研究螺旋藻对NH4-N的处理效率,着重探讨利用生活污水培养螺旋藻的可行性,及其对生活污水的处理效果。研究证明,在NH4-N浓度小于40.0mg/L时,其对NH4-N的去除率最大,可达93％,左右。在体积百分比生活污水为80％时,添加NaHCO38mg/L,对NH4-N的去除率达91.8％。同时对螺旋藻化学组分及含铁、锌、锰、铜、硒进行分析,生活污水培养螺旋藻,其所含有机物和氮源,可供螺旋藻生长繁殖,使废水资源化,并减少环境污染;藻粉可作为饲料添加剂,是使废水变为蛋白源的有效途径。

钝顶螺旋藻不同形态藻丝体的蛋白质表达和生理差异的比较研究

无论是在生产还是在实验室培养条件下,螺旋藻多形性变异现象普遍存在.本研究从钝顶螺旋藻(Spirulina platensis)中分离纯化获得了4种不同形态的藻丝体,分别是直线形藻丝体(spL)、低度螺旋形藻丝体(sps-L)、中度螺旋形藻丝体(sps-M)和高度螺旋形藻丝体(sps-H).对这4种不同形态螺旋藻的生长速率、光合作用、叶绿素a(Chl a)含量分别进行研究,发现这4种不同形态藻丝体在生长和光合生理上存在明显的差异.而总蛋白的SDS-PAGE分析也表明,不同形态藻丝体在蛋白的表达上也有差异,其中34 ku的蛋白只在直线形藻丝体spL中大量表达.

正交实验法优选钝顶螺旋藻的培养条件

研究了钝顶螺旋藻的培养条件,通过正交实验考察了pH值、光照度、温度和振荡速度对螺旋藻生长的影响.结果表明:pH值 9～10、光照度6000 lx、温度 33℃、转速 130 r·min-1、维生素B6加入量 5×10-5g·L-1时,钝顶螺旋藻生长良好,培养7 d,干重为0.6112 g·L-1.

螺旋藻品系Z中不同形态藻丝体的生长速率和光合色素比较

The line-shaped filaments Sp-Z(L) were isolated and cultured from Spirulina platensis Sp-Z. The growth rate of Sp-Z(L) was only 64% as much as that of SP-Z when the light intensity was 4000 Lux. The contentS (×10-3 g / g dry weight) of chlorophylls, carotenoids and phycobilins of Sp-Z(L) and Sp-Z were 20.6, 0.343, 5.00 and 24.1 0.297, 4.46, respectively. Moreover, as to the absorption spectra of the three photopigments of SP-Z(L), red shifts were observed. Therefore, after the spiral Sp-Z breeded or changed into Sp-Z(L), The contentS of carotenoids and phycobilins raised 23% and 12%, respectively, but the contents of chlorophylls dropped 14.5%, and the growth rate dropped 36% too. The results probably showed that the decrease of chlorophylls was one of the main reasons that caused the remarkable decrease of the growth rate of SP-Z(L).

初始氨氮浓度对钝顶螺旋藻生长及其去除率的影响

螺旋藻是一种经济价值很高的微藻,研究氨氮对钝顶螺旋藻生长及其去除水中氨氮效率的影响,探讨其在废水处理中的可行性具有重要意义。结果表明,以硫酸铵作为氮源时,钝顶螺旋藻的对数期一般在4～6 d,随着氨氮浓度的升高对数期略有提前。钝顶螺旋藻对硫酸氨的耐受浓度为0.5 g/L,相应的氨氮浓度为106 mg/L。培养4～6 d时氨氮浓度下降幅度最大,培养结束时氨氮去除率为67.7%～82.5%,当硫酸铵投加量为0.4 g/L时,氨氮去除率最高,达82.6%。因此,螺旋藻可用于去除废水中的氨氮,具有很好的应用前景。

光照度和温度对螺旋藻生长速率及品质的影响

为寻求优质、高产螺旋藻的生态条件,以 spirulina platensis 为藻种,以 zarrouk 为培养基,通过通气培养,探讨了温度、光照度和接种密度对螺旋藻生长速率及品质的影响,从而找到温度和光照度的最佳组合,并确定出合适的接种密度。在夏季,接种密度应提高到0.4～0.6,冬季应降低到0.2左右。