To establish the rapid and accurate methods for detecting cancer cells from the sputum of patients with lung cancer. Methods: Sputum sediment section examination and sputum smear examination were performed. For 142 ca...To establish the rapid and accurate methods for detecting cancer cells from the sputum of patients with lung cancer. Methods: Sputum sediment section examination and sputum smear examination were performed. For 142 cases of lung cancer patients diagnosed by bronchia fibrous lens and confirmed by pathologic diagnosis. Results: (a) The positive diagnosis rate of lung cancer cell was 71.83% (102/142) when using sputum sediment examination while that was 32.39% (46/142) when using sputum smear examination, when using sputum sediment examination jointly with sputum smear examination, the positive diagnosis rate of lung cancer was 91.55% (130/142). According to the positive diagnosis rate of lung cancer, the sputum sediment examination was significantly higher than sputum smear examination (P<0.001), joint examination of both of them was significantly higher than the single sputum sediment examination (P<0.001); (b) As to the histopathological types of lung cancers, 66 cases could be identified among 102 cases whom were confirmed by sputum sediment examination, the identification rate was 64.71%; and 8 cases could be identified among 46 cases whom were confirmed by sputum smear examination, with an identification rate of 17.39%, it was significantly lower than that by sputum sediment examination (P<0.001); (c) Immunohistochemistry technique could be used in the sections of sputum sediment to identify some histopathological types which could not be identified by routine HE stain, with an identification rate of 94.44% (34/36). Conclusion: the sputum sediment examination has some advantages compared to the sputum smear examination such as the specimen may include more materials, the higher cancer cell identification positive rate and immunohistochemistry method can be used to identify the histopathological type.展开更多
文摘To establish the rapid and accurate methods for detecting cancer cells from the sputum of patients with lung cancer. Methods: Sputum sediment section examination and sputum smear examination were performed. For 142 cases of lung cancer patients diagnosed by bronchia fibrous lens and confirmed by pathologic diagnosis. Results: (a) The positive diagnosis rate of lung cancer cell was 71.83% (102/142) when using sputum sediment examination while that was 32.39% (46/142) when using sputum smear examination, when using sputum sediment examination jointly with sputum smear examination, the positive diagnosis rate of lung cancer was 91.55% (130/142). According to the positive diagnosis rate of lung cancer, the sputum sediment examination was significantly higher than sputum smear examination (P<0.001), joint examination of both of them was significantly higher than the single sputum sediment examination (P<0.001); (b) As to the histopathological types of lung cancers, 66 cases could be identified among 102 cases whom were confirmed by sputum sediment examination, the identification rate was 64.71%; and 8 cases could be identified among 46 cases whom were confirmed by sputum smear examination, with an identification rate of 17.39%, it was significantly lower than that by sputum sediment examination (P<0.001); (c) Immunohistochemistry technique could be used in the sections of sputum sediment to identify some histopathological types which could not be identified by routine HE stain, with an identification rate of 94.44% (34/36). Conclusion: the sputum sediment examination has some advantages compared to the sputum smear examination such as the specimen may include more materials, the higher cancer cell identification positive rate and immunohistochemistry method can be used to identify the histopathological type.
