Influence of several uropathogenic microorganisms on human sperm motility parameters in vitro

Aim: The effects of certain uropathogenic microorganisms (Neisseria gonorrhoeae, Staphylococcus aureus, Staphylococcus epidermidis and Mycobacterium tuberculosis) on human sperm motility characteristics were studied in vitro. Methods: In 10 healthy fertile men, ejaculates were aseptically obtained by masturbation and With a swim-up technique, a sperm suspension of high motility and purity was obtained. Several uropathogenic bacteria were obtained from outpatients with genitourinary tract infections. The sperm suspension was incubated with the pathogens at a bacteria: sperm ratio of 50:1 at 37℃. The sperm mobility parameters were estimated with a computerassisted sperm analyzer (CASA) provided with a multiple-exposure photography system (Madi Corp., Zhejiang, China). Measurements were carried out at 0, 2 and 4 hours of incubation. Results: Staphylococcus aureus significantly decreased the sperm motility and viability, but Staphylococcus epidermidis, Mycobacterium tuberculosis and Neisseria gonorrhoeae did not. Conclusion: Staphylococcus aureus has an inhibitory effect on human sperm motility in vitro.

金黄色葡萄球菌和表皮葡萄球菌体外对人精子运动功能的影响

目的 :研究金黄色葡萄球菌 (SA)和表皮葡萄球菌 (SE)体外对人精子的运动功能有无直接影响。 方法 :将泌尿生殖系感染病人中分离培养的SA和SE制成活菌悬液 ,体外与 10例健康成年男性手淫获得并经上游优化处理的精子孵育。按细菌 /精子比 5 0∶1分别孵育 0、2、4h后 ,采用计算机辅助的精子分析系统检测人精子活率、运动参数 (前向性运动百分率、直线速度、曲线速度、平均路径速度 )和精子形态及凝集情况。 结果 :SA与精子体外孵育 2、4h后 ,精子活率和运动参数明显降低 (P <0 .0 5 )。SE体外对精子的活率和运动功能则无明显影响。 结论 :SA与精子的比例为 5 0∶1,体外孵育 2h后 ,能显著降低精子活率和抑制精子运动功能。SE对精子运动功能无直接影响。

不同致病菌体外对人精子运动参数的影响

目的 为了研究金黄色葡萄球菌、表皮葡萄球菌和结核杆菌体外对人精子的运动功能有无直接影响。方法 将从临床泌尿生殖系感染病人中分离培养出的金黄色葡萄球菌、表皮葡萄球菌和结核杆菌制成活菌悬液 ,然后将其在体外与 10例健康成年男性手淫获得并经上游优化处理的精子按菌 精比 5 0 :1一起孵化 0、2和 4小时后 ,采用计算机辅助的精子分析系统检测人精子活率、运动参数 (前向性运动百分率、直线速度VCL、曲线速度VSL、平均路径速度VAP)和精子形态及凝集情况。结果 金黄色葡萄球菌与精子比例为 5 0 :1体外孵化 0、2和 4小时后 ,人精子活率和运动参数明显降低 (P <0 .0 5 )。表皮葡萄球菌和结核杆菌体外对人精子的活率和运动功能则无明显影响。结论 金黄色葡萄球菌与精子的比例为 5 0 :1体外孵化 4小时内 ,能显著降低人精子活率和抑制精子运动功能。