Biofilms are a constant concern in the food industry;understanding the effect of environmental conditions on biofilm formation is essential to develop effective control strategies.Therefore,this study was conducted to...Biofilms are a constant concern in the food industry;understanding the effect of environmental conditions on biofilm formation is essential to develop effective control strategies.Therefore,this study was conducted to investigate biofilms formation by Staphylococcus epidermidis under various conditions.Biofilms were cultured in nutrient broth containing different carbon source concentrations(0–10 mg/mL)on polystyrene surfaces for 32 h of incubation at 37℃or 55℃,with quantification and enumeration at 8,16,24 and 32 h.S.epidermidis developed biofilms under all tested conditions;achieved the highest yield of biofilm biomass at 2.5 mg/mL for all carbon sources at 37℃.The highest efficiency of extracellular polymeric substance(EPS)molecule production occurred under glucose availability in the growth environment,with a higher yield of biomass and a significantly smaller number of metabolically active cells than under other tested conditions.A condensed ball-shaped structure was observed under the lactose condition.Meanwhile,biofilms in the presence of maltose showed mainly opaque thick rich colonies,while a compact multilayered-shaped structure was exhibited under both glucose and sucrose conditions.These results contribute to a better understanding of the biofilm formation by S.epidermidis in order to reduce contamination and recontamination in the food industry.展开更多
Bacterial biofilms have been implicated with breast implant complications including capsular contracture, double-capsule formation, and breast implant-associated anaplastic large cell lymphoma. However, the relationsh...Bacterial biofilms have been implicated with breast implant complications including capsular contracture, double-capsule formation, and breast implant-associated anaplastic large cell lymphoma. However, the relationship between implant surface texture and microbial biofilm formation is insufficiently evaluated. In the present study, we examined the antimicrobial activities of different types of silicone breast implant. The growth of bacterial including <em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>, and <em>Pseudomonas aeruginosa</em> was compared using implants with various surface textures, including Hans Smooth, Hans SmoothFine, Allergan Smooth, Eurosilicone Smooth, Eurosilicone Texture, Sebbin Smooth, Sebbin Micro, Sebbin Texture, and Motiva Smooth. Microbial investigation revealed the increased growth of <em>S. aureus</em> on breast implants after 48 h, except Eurosilicone Smooth, Eurosilicone Texture, Hans SmoothFine and Sebbin Smooth material. At 48 hours, there was no major difference between the <em>S. aureus</em> attachment on smooth and textured implants. The results of <em>S. epidermis</em> attachment on the implant after 48 h showed that their growth decreased on surfaces of Motiva Smooth, Sebbin Smooth, and Eurosilicone Smooth. These results indicated that <em>S. epidermis</em> was unable to survive on these breast implants. Eventually, <em>P. aeruginosa</em> count had showed decrease of bacterial count after 48 hours compared to 24 hours in most of the implants except for Eurosilicone Texture, Sebbin Smooth and Sebbin Micro, where the count of <em>P. aeruginosa</em> slightly increased. This indicated that <em>P. aeruginosa</em> was unable to exist on the smooth surfaces. Our results show that the in vitro assay revealed no significant difference between smooth and textured surfaces and showed variable interactions and needed further molecular analysis to assess their adherence nature.展开更多
基金This work was financially supported by the National Key Research and Development Program of China(2016YFD0400301)the Key Research and Development Program of Zhejiang Province(2017C02015).
文摘Biofilms are a constant concern in the food industry;understanding the effect of environmental conditions on biofilm formation is essential to develop effective control strategies.Therefore,this study was conducted to investigate biofilms formation by Staphylococcus epidermidis under various conditions.Biofilms were cultured in nutrient broth containing different carbon source concentrations(0–10 mg/mL)on polystyrene surfaces for 32 h of incubation at 37℃or 55℃,with quantification and enumeration at 8,16,24 and 32 h.S.epidermidis developed biofilms under all tested conditions;achieved the highest yield of biofilm biomass at 2.5 mg/mL for all carbon sources at 37℃.The highest efficiency of extracellular polymeric substance(EPS)molecule production occurred under glucose availability in the growth environment,with a higher yield of biomass and a significantly smaller number of metabolically active cells than under other tested conditions.A condensed ball-shaped structure was observed under the lactose condition.Meanwhile,biofilms in the presence of maltose showed mainly opaque thick rich colonies,while a compact multilayered-shaped structure was exhibited under both glucose and sucrose conditions.These results contribute to a better understanding of the biofilm formation by S.epidermidis in order to reduce contamination and recontamination in the food industry.
文摘Bacterial biofilms have been implicated with breast implant complications including capsular contracture, double-capsule formation, and breast implant-associated anaplastic large cell lymphoma. However, the relationship between implant surface texture and microbial biofilm formation is insufficiently evaluated. In the present study, we examined the antimicrobial activities of different types of silicone breast implant. The growth of bacterial including <em>Staphylococcus aureus</em>, <em>Staphylococcus epidermidis</em>, and <em>Pseudomonas aeruginosa</em> was compared using implants with various surface textures, including Hans Smooth, Hans SmoothFine, Allergan Smooth, Eurosilicone Smooth, Eurosilicone Texture, Sebbin Smooth, Sebbin Micro, Sebbin Texture, and Motiva Smooth. Microbial investigation revealed the increased growth of <em>S. aureus</em> on breast implants after 48 h, except Eurosilicone Smooth, Eurosilicone Texture, Hans SmoothFine and Sebbin Smooth material. At 48 hours, there was no major difference between the <em>S. aureus</em> attachment on smooth and textured implants. The results of <em>S. epidermis</em> attachment on the implant after 48 h showed that their growth decreased on surfaces of Motiva Smooth, Sebbin Smooth, and Eurosilicone Smooth. These results indicated that <em>S. epidermis</em> was unable to survive on these breast implants. Eventually, <em>P. aeruginosa</em> count had showed decrease of bacterial count after 48 hours compared to 24 hours in most of the implants except for Eurosilicone Texture, Sebbin Smooth and Sebbin Micro, where the count of <em>P. aeruginosa</em> slightly increased. This indicated that <em>P. aeruginosa</em> was unable to exist on the smooth surfaces. Our results show that the in vitro assay revealed no significant difference between smooth and textured surfaces and showed variable interactions and needed further molecular analysis to assess their adherence nature.
