We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis （Fabricius）. A leaf-sandwich method was applied to test the bioactivity of the secretion. Co...We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis （Fabricius）. A leaf-sandwich method was applied to test the bioactivity of the secretion. Coomassie brilliant blue, gas chromatography （GC） and other methods were used to analyze and carry out protein assays, amino acid analyses, fatty acid and cholesterol detection of the secretion. Results show that the secretion had a significant antifeeding effect on 4th instar larvae of the armyworm （Pseudaletia separata Walker）. Dissolved in 0.27 mol.L^-1 HCl, 0.1 mol.L^-1 NaOH and 0.05 mol.L^-1 Na2HPO4 separately, the secretions had antifeeding rates ranging from 22.7% to 49.6%. Meanwhile, the weights of the secretion-treated insects were significantly lower than those of the control. Biochemical detection showed that the proteins contained in the secretion were composed of 16 amino acids. The secretion also contained small amounts of cholesterol and fatty acids, which included lauric acid, myristic acid, myristoleic acid, seventeen carbonic acids and oleic acid.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 30872032).
文摘We analyzed the composition and studied the bioactivity of secretion from the poplar sawfly, Stauronematus compressicornis （Fabricius）. A leaf-sandwich method was applied to test the bioactivity of the secretion. Coomassie brilliant blue, gas chromatography （GC） and other methods were used to analyze and carry out protein assays, amino acid analyses, fatty acid and cholesterol detection of the secretion. Results show that the secretion had a significant antifeeding effect on 4th instar larvae of the armyworm （Pseudaletia separata Walker）. Dissolved in 0.27 mol.L^-1 HCl, 0.1 mol.L^-1 NaOH and 0.05 mol.L^-1 Na2HPO4 separately, the secretions had antifeeding rates ranging from 22.7% to 49.6%. Meanwhile, the weights of the secretion-treated insects were significantly lower than those of the control. Biochemical detection showed that the proteins contained in the secretion were composed of 16 amino acids. The secretion also contained small amounts of cholesterol and fatty acids, which included lauric acid, myristic acid, myristoleic acid, seventeen carbonic acids and oleic acid.
