A new biflavonoid, stelleranol, was isolated from the roots of Stellera chamaejasme L.. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.
Methanol extract of Stellera chamaejasme L wasassessed for antitumor activity by an antitumor activebioassay against murine leukemia P388 in vivo. Thebioassay-directed separation of the extract furnishedseven diterpen...Methanol extract of Stellera chamaejasme L wasassessed for antitumor activity by an antitumor activebioassay against murine leukemia P388 in vivo. Thebioassay-directed separation of the extract furnishedseven diterpene compounds (stellerarin, stelleramacrin,gnidimacrin, pimelea factor P2, subtoxin, huratoxin,simplexin ) and two biflavanone compounds (neochemae-jasmin A and B). Among them, gnidimacrin, stellerarinand stelleramacrin (a novel compound) were found tohave high antitumor and cytotoxic activities against P388,L1210 and K562 in vivo and in vitro. The results suggestedthat the diterpene compounds were the potent anti-tumor principles of Stellera chamaejame L展开更多
Steller chamaejasme L. （S. chamaejasme for short） is one of the most noxious unpalatable weeds in China, which has been frequently reported its negative interaction （i.e. competition and allelopathy） with other he...Steller chamaejasme L. （S. chamaejasme for short） is one of the most noxious unpalatable weeds in China, which has been frequently reported its negative interaction （i.e. competition and allelopathy） with other herbaceous species in grasslands. This study compared species diversity, biomass and sexual reproduction of herbaceous plants in meadows with S. chamaejasme and in open meadows without S. ehamaejasme in overgrazing meadows on the Tibetan Plateau in China to determine whether positive facilitation exist between S. chamaejasme and other herbaceous species under livestock＇s overgrazing. The results showed that there are more herbaceous species in meadows with S. chamaejasme than those in open meadows （35s and30s, respectively）. Diversity index and above-ground biomass were also significantly higher in meadows with S. charnaejasme. There were 39% （11/28） of all species with sexual reproduction found in meadows with S. charnaejasme, which was 7 times more than those in open meadows. Our study showed that S. charnaejasme could provide biotic refuge for neighboring plants and preserve plant diversity from livestock＇s overgrazing in alpine meadows on the Tibetan Plateau. It also suggested that inter-specific facilitation between S. charnaejasme and other herbaceous species may play a key role in overgrazing alpine meadows.展开更多
Gnidimacrin, a diterpene compound, isolated from the methanol extract of Stellera, chamaejasme L., showed significant antitumor activities against mouse leukemia P-388 and L-1210 in vivo, such as Lewis lung carcinoma,...Gnidimacrin, a diterpene compound, isolated from the methanol extract of Stellera, chamaejasme L., showed significant antitumor activities against mouse leukemia P-388 and L-1210 in vivo, such as Lewis lung carcinoma, B-16 melanoma and Colon cancer 26. It showed ILSs of 40%.49% and 41% at the dosages of 0.01-0.02 mg/kg ip,respectively. Gnidimacrin strongly inhibited cell Proliferation of human cancer cell lines such as leukemia K562, stomach cancers Kato-III, MKN-28, MKN-45, and mouse leukemia L-1210 by the MTT assay and colony forming assay in vitro. The IC50 of gnidimacrin was 0.007-0.00012 μg/ml. It is concluded that gnidimacrin is the principal antitumor element in Stellera chemaejasme L. with strong antitumor activities.展开更多
A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots o...A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots of Stellera chamaejasme L. together with known chamaechromone. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.展开更多
目的研究瑞香狼毒水提取物(S tellera chamaejasme L inn,SCLA)对人膀胱癌Biu-87细胞(Biu-87 human bladder cancer cell,Biu-87)的细胞毒性作用及对B淋巴细胞瘤/白血病-2(B-celllymphoma/leukemia-2,Bcl-2)蛋白表达的影响,进而讨论瑞...目的研究瑞香狼毒水提取物(S tellera chamaejasme L inn,SCLA)对人膀胱癌Biu-87细胞(Biu-87 human bladder cancer cell,Biu-87)的细胞毒性作用及对B淋巴细胞瘤/白血病-2(B-celllymphoma/leukemia-2,Bcl-2)蛋白表达的影响,进而讨论瑞香狼毒水提取物对人膀胱癌细胞增殖的抑制机制。方法用瑞香狼毒水提取物作用于人膀胱癌Biu-87细胞,观察不同药物浓度,不同作用时间的细胞增殖情况。以WST-1细胞增殖及细胞毒性检测试剂盒(WST-1 Cell Proliferation and Cytotoxicity Assay Kit,WST-1)检测瑞香狼毒水提取物对膀胱癌细胞生长的抑制率;流式细胞术检测人膀胱癌Biu-87细胞凋亡情况;蛋白质印迹法(Western blot,WB)测定不同浓度瑞香狼毒水提取物作用后的人膀胱癌Biu-87细胞中Bcl-2蛋白的表达情况。结果经瑞香狼毒水提取物作用后人膀胱癌Biu-87细胞的增殖受到抑制;该水提取物促进人膀胱癌Biu-87细胞凋亡;Bcl-2蛋白在人膀胱癌Biu-87细胞的表达随药物浓度的增加而减少。结论瑞香狼毒水提取物可抑制人膀胱癌Biu-87细胞增殖,通过减少Bcl-2的表达从而抑制该细胞株增殖,促进其凋亡。随着药物浓度的增加、作用时间的延长,对人膀胱癌Biu-87细胞的抑制作用更加明显。展开更多
文摘A new biflavonoid, stelleranol, was isolated from the roots of Stellera chamaejasme L.. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.
文摘Methanol extract of Stellera chamaejasme L wasassessed for antitumor activity by an antitumor activebioassay against murine leukemia P388 in vivo. Thebioassay-directed separation of the extract furnishedseven diterpene compounds (stellerarin, stelleramacrin,gnidimacrin, pimelea factor P2, subtoxin, huratoxin,simplexin ) and two biflavanone compounds (neochemae-jasmin A and B). Among them, gnidimacrin, stellerarinand stelleramacrin (a novel compound) were found tohave high antitumor and cytotoxic activities against P388,L1210 and K562 in vivo and in vitro. The results suggestedthat the diterpene compounds were the potent anti-tumor principles of Stellera chamaejame L
基金funded by the National Natural Science Foundation of China (Grant Nos. 31100360, 31200378, 31000233)
文摘Steller chamaejasme L. （S. chamaejasme for short） is one of the most noxious unpalatable weeds in China, which has been frequently reported its negative interaction （i.e. competition and allelopathy） with other herbaceous species in grasslands. This study compared species diversity, biomass and sexual reproduction of herbaceous plants in meadows with S. chamaejasme and in open meadows without S. ehamaejasme in overgrazing meadows on the Tibetan Plateau in China to determine whether positive facilitation exist between S. chamaejasme and other herbaceous species under livestock＇s overgrazing. The results showed that there are more herbaceous species in meadows with S. chamaejasme than those in open meadows （35s and30s, respectively）. Diversity index and above-ground biomass were also significantly higher in meadows with S. charnaejasme. There were 39% （11/28） of all species with sexual reproduction found in meadows with S. charnaejasme, which was 7 times more than those in open meadows. Our study showed that S. charnaejasme could provide biotic refuge for neighboring plants and preserve plant diversity from livestock＇s overgrazing in alpine meadows on the Tibetan Plateau. It also suggested that inter-specific facilitation between S. charnaejasme and other herbaceous species may play a key role in overgrazing alpine meadows.
文摘Gnidimacrin, a diterpene compound, isolated from the methanol extract of Stellera, chamaejasme L., showed significant antitumor activities against mouse leukemia P-388 and L-1210 in vivo, such as Lewis lung carcinoma, B-16 melanoma and Colon cancer 26. It showed ILSs of 40%.49% and 41% at the dosages of 0.01-0.02 mg/kg ip,respectively. Gnidimacrin strongly inhibited cell Proliferation of human cancer cell lines such as leukemia K562, stomach cancers Kato-III, MKN-28, MKN-45, and mouse leukemia L-1210 by the MTT assay and colony forming assay in vitro. The IC50 of gnidimacrin was 0.007-0.00012 μg/ml. It is concluded that gnidimacrin is the principal antitumor element in Stellera chemaejasme L. with strong antitumor activities.
文摘A new chromone derivative, 3-[1- (2, 4, 6-trihydroxyphenyl) 3-di-(4-hydroxyphenyl) 1-propanone-2-yl] 5,7-dihydroxy-8-di(4-hydroxyphenyl)methyl-4H-1-benzopyran-4-one, named as isomohsenone was isolated from the roots of Stellera chamaejasme L. together with known chamaechromone. Its structure was determined by the analysis of MS and NMR data, especially 2D NMR spectra.
文摘目的研究瑞香狼毒水提取物(S tellera chamaejasme L inn,SCLA)对人膀胱癌Biu-87细胞(Biu-87 human bladder cancer cell,Biu-87)的细胞毒性作用及对B淋巴细胞瘤/白血病-2(B-celllymphoma/leukemia-2,Bcl-2)蛋白表达的影响,进而讨论瑞香狼毒水提取物对人膀胱癌细胞增殖的抑制机制。方法用瑞香狼毒水提取物作用于人膀胱癌Biu-87细胞,观察不同药物浓度,不同作用时间的细胞增殖情况。以WST-1细胞增殖及细胞毒性检测试剂盒(WST-1 Cell Proliferation and Cytotoxicity Assay Kit,WST-1)检测瑞香狼毒水提取物对膀胱癌细胞生长的抑制率;流式细胞术检测人膀胱癌Biu-87细胞凋亡情况;蛋白质印迹法(Western blot,WB)测定不同浓度瑞香狼毒水提取物作用后的人膀胱癌Biu-87细胞中Bcl-2蛋白的表达情况。结果经瑞香狼毒水提取物作用后人膀胱癌Biu-87细胞的增殖受到抑制;该水提取物促进人膀胱癌Biu-87细胞凋亡;Bcl-2蛋白在人膀胱癌Biu-87细胞的表达随药物浓度的增加而减少。结论瑞香狼毒水提取物可抑制人膀胱癌Biu-87细胞增殖,通过减少Bcl-2的表达从而抑制该细胞株增殖,促进其凋亡。随着药物浓度的增加、作用时间的延长,对人膀胱癌Biu-87细胞的抑制作用更加明显。
