Rice bacterial leaf streak,caused by Xanthomonas oryzae pv.oryzicola,is an important bacterial disease in rice-planting areas in South China and Southeast Asian countries.It occurs every year in local areas.This paper...Rice bacterial leaf streak,caused by Xanthomonas oryzae pv.oryzicola,is an important bacterial disease in rice-planting areas in South China and Southeast Asian countries.It occurs every year in local areas.This paper reviews the research advance in the occurrence and harms of rice bacterial leaf streak in China,classification of Xanthomonas oryzae and infection characteristics,pathogenic factors and pathogenicity of rice bacterial leaf streak and breeding of bacterial leaf streakresistance rice cultivars.Some issues involved in occurrence and control of rice bacterial leaf streak were presented,and the areas on which the future studies might be focused were discussed.展开更多
建立液相色谱-串联质谱技术测定牛排产品中大豆蛋白含量的方法。首先利用高分辨质谱结合Unitprot数据库,对大豆特异性多肽进行鉴别及筛选,获得可用于大豆准确定性的多肽序列信息,并利用skyline软件将多肽序列转换为离子对信息;其次利用...建立液相色谱-串联质谱技术测定牛排产品中大豆蛋白含量的方法。首先利用高分辨质谱结合Unitprot数据库,对大豆特异性多肽进行鉴别及筛选,获得可用于大豆准确定性的多肽序列信息,并利用skyline软件将多肽序列转换为离子对信息;其次利用液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)对大豆蛋白特异性多肽进行确证,构建大豆蛋白LC-MS/MS定性判别方法;进一步开展用于定量多肽的筛选研究,最终筛选到线性和回收率较好的定量多肽共3条,分别为GSDLVNVR、VSDDEFNNYK、LVFCPQQAEDDK,线性相关系数均达到0.99以上,加标回收率为81.7%~115.8%,精密度小于13%,检出限为0.15%,定量限为0.5%;同时对市售28个牛排进行大豆蛋白含量的测定,构建LC-MS/MS方法测定牛排中大豆蛋白含量的方法。结果表明,该方法特异性好,准确度高,同时可推广应用至其他肉制品。展开更多
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201303015201103002-3)+2 种基金Natural Science Foundation of Jiangsu Province(BK20130713)Jiangsu Agricultural Science and Technology Innovation Fund(CX(12)3058)National High Technology Research and Development Program of China(863Program)(2011AA10A201)~~
文摘Rice bacterial leaf streak,caused by Xanthomonas oryzae pv.oryzicola,is an important bacterial disease in rice-planting areas in South China and Southeast Asian countries.It occurs every year in local areas.This paper reviews the research advance in the occurrence and harms of rice bacterial leaf streak in China,classification of Xanthomonas oryzae and infection characteristics,pathogenic factors and pathogenicity of rice bacterial leaf streak and breeding of bacterial leaf streakresistance rice cultivars.Some issues involved in occurrence and control of rice bacterial leaf streak were presented,and the areas on which the future studies might be focused were discussed.
文摘建立液相色谱-串联质谱技术测定牛排产品中大豆蛋白含量的方法。首先利用高分辨质谱结合Unitprot数据库,对大豆特异性多肽进行鉴别及筛选,获得可用于大豆准确定性的多肽序列信息,并利用skyline软件将多肽序列转换为离子对信息;其次利用液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)对大豆蛋白特异性多肽进行确证,构建大豆蛋白LC-MS/MS定性判别方法;进一步开展用于定量多肽的筛选研究,最终筛选到线性和回收率较好的定量多肽共3条,分别为GSDLVNVR、VSDDEFNNYK、LVFCPQQAEDDK,线性相关系数均达到0.99以上,加标回收率为81.7%~115.8%,精密度小于13%,检出限为0.15%,定量限为0.5%;同时对市售28个牛排进行大豆蛋白含量的测定,构建LC-MS/MS方法测定牛排中大豆蛋白含量的方法。结果表明,该方法特异性好,准确度高,同时可推广应用至其他肉制品。